Plasmid system for multigene expression

FIG. 1

ft {■*■} artist

"i

pULLS

1 tjl D r )

Col E1 ortgm

i

T7 promoter/priming site

Kappa
Beta globtn pA signal
ft7d(lG094)
AWI (10077)
flrfBI(9857).

TK/Hyg
Afcml(8403)

Beta Globin PolyA signal
&/I(8144)

IGG4-CH3
IGG4-CH2

S/fI(11913)
SjrAI (11919)
Hpal (12075)

SV40 POLYA
SV40 t Ag Intron
DHFR cONA

MMW-LTR
ArII(2763)
fi»RV(2975)
£co47III(3022)
pBR ORi
pBR322

APr >tecl(5045) CMV

T7 promoter/priming site

IGG4-CH1