Methods and compositions for poly-

(54) METHODS AND COMPOSITIONS FOR
POLY-p-l-4-N-ACETYLGLUCOSAMINE
CELL THERAPY SYSTEM

(75) Inventors: John N. Vournakis, Hanover, NH
(US); Sergio Finkielstein, Chestnut
Hill, MA (US); Ernest R. Pariser,
Belmont, MA (US); Mike Helton,
Memphis, TN (US)

(73) Assignee: Marine Polymer Technologies, Inc.,

Danvers, MA (US)

( * ) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 0 days.

(21) Appl. No.: 10/005,130

(22) Filed: Dec. 5, 2001

(65) Prior Publication Data

US 2002/0106792 Al Aug. 8, 2002

Related U.S. Application Data

(63) Continuation of application No. 09/866,827, filed on May 29, 2001, which is a continuation of application No. 09/227, 840, filed on Jan. 11, 1999, now abandoned, which is a division of application No. 08/471,290, filed on Jun. 6,1995, now Pat. No. 5,858,350, which is a continuation-in-part of application No. 08/347,911, filed on Dec. 1, 1994, now Pat. No. 5,623,064, which is a continuation-in-part of application No. 08/160,569, filed on Dec. 1, 1993, now Pat. No. 5,622, 834.

(51) Int. CI.7 A61K 31/715; A61K 31/722

(52) U.S. CI 514/55; 514/54; 514/62

(58) Field of Search 514/54, 55, 62

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Primary Examiner—Kathleen K. Fonda

(74) Attorney, Agent, or Firm—Pennie & Edmonds LLP

(57) ABSTRACT

The present invention relates to a purified, easily produced poly-(3-l—»4-N-acetylglucosamine (p-GlcNAc) polysaccharide species. The p-GlcNAc of the invention is a polymer of high molecular weight whose constituent monosaccharide sugars are attached in a (3-1—»4 conformation, and which is free of proteins, and substantially free of single amino acids, and other organic and inorganic contaminants. In addition, derivatives and reformulations of p-GlcNAc are described. The present invention further relates to methods for the purification of the p-GlcNAc of the invention from microalgae, preferably diatom, starting sources. Still further, the invention relates to methods for the derivatization and reformulation of the p-GlcNAc. Additionally, the present invention relates to the uses of pure p-GlcNAc, its derivatives, and/or its reformulations.

4 Claims, 57 Drawing Sheets

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