A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a chromatographic zone on which is disposed a plurality of microporous particles. The chromatographic zone can effectively reduce the “hook effect” in a simple,...http://www.google.ca/patents/US7662643?utm_source=gb-gplus-sharePatent US7662643 - Reduction of the hook effect in membrane-based assay devices