WO2014058172A1 - Device for selectively injecting liquid into reaction vessel and biosensor including same - Google Patents

Abstract

The device for selectively injecting a liquid into a reaction vessel, according to the present invention, comprises: a housing which is connected to the reaction vessel and which has a plurality of inflow passages, through which the liquid is injected, and a plurality of flow paths, which correspond to and communicate with the plurality of inflow passages, respectively; a connection passage which is connected to the plurality of flow paths and which is formed through the inner portion of the housing along the lengthwise direction from the central axis which is separated by a predetermined distance from the plurality of inflow passages; and a flow path selection means including a connection groove, which is incorporated inside the connection passage and connected to the housing in a rotatable manner, and which communicates with one of the plurality of flow paths selected in response to a rotation with respect to the central axis, and an outlet which communicates with the connection groove and the reaction vessel and which discharges the liquid flowing in through the selected flow path and the connection groove into the reaction vessel.

Description

Apparatus for selectively injecting liquid into the reaction vessel and biosensor comprising the same

The present invention relates to a device for selectively injecting a liquid into a reaction vessel and to a biosensor comprising the same, and more particularly, to selectively and several liquids to be reacted to a reaction vessel in which a biological, biochemical or chemical reaction is carried out. An apparatus for sequentially injecting and a biosensor comprising the same.

Specifically, the present invention is to selectively and sequentially a plurality of liquids, for example, deionized water, reaction solution and the liquid sample to be tested in the reaction vessel in which the biochip embedded in the biosensor, such as DNA analysis device is equipped An apparatus for injecting and a biosensor comprising the same.

Biosensors are devices that can analyze gene information and protein information in large quantities and automatically, or can analyze the presence and function of bioactive substances relatively simply and quickly. Such biosensors are actively applied in various fields such as gene and protein research, medicine, agriculture, food, environment and chemical industry.

Such biosensors include a microfluidics chip, a so-called biochip, which checks for the presence and / or reaction of a bioactive substance in a sample to be tested, and includes a substance to be tested (eg, DNA, RNA, peptide). It is a device for analyzing the reaction pattern of the various bioactive substances in the biochip while flowing a liquid sample containing a (), protein, etc.) to the reaction vessel containing the biochip. Such a biosensor detects a change in electrical characteristics according to a reaction of a bioactive substance in a reaction vessel and detects the presence and / or reaction of the bioactive substance by sensing an electrode installed in the biochip.

The new technology is being improved in connection with the biosensor as described above, and in the biosensor field to which the present invention belongs, as in other technical fields, continuous improvement of the technology is required. For example, there is also a demand for improvement of a technique for efficiently injecting a reaction solution, a test target liquid sample, and the like into a reaction vessel having a biochip embedded therein according to a reaction sequence.

In this regard, Korean Patent Laid-Open Publication No. 10-2011-0075448 discloses a sample inlet for injecting a liquid sample into a reaction space formed between an upper substrate and a lower substrate, and a sample charging portion filled with a liquid sample injected through the sample inlet. And a sample outlet for discharging the reaction solution after completion of the reaction. In addition, Korean Patent Laid-Open No. 10-2012-0056442 discloses a sample inlet, a sample reaction part, a sample discharge part, a first microfluidic channel connecting a sample inlet part and a sample reaction part into which a biological sample is injected, and a sample reaction part. A reaction chamber comprising a second microfluidic channel connecting a sample outlet is disclosed.

However, the prior art as described above is only configured to inject a liquid sample into the reaction chamber containing the biochip through the microchannel and to discharge the reaction solution from the reaction chamber containing the biochip through the microchannel after completion of the reaction. have. Therefore, in the prior art, when it is necessary to inject various reaction solutions and the liquid sample to be tested into the reaction chamber in which the biochip is built according to the reaction sequence, the reaction solution and the liquid sample are exchanged before and after the reaction and manually injected into the reaction chamber. There was a inconvenience that the reaction solution and the liquid sample could not be efficiently injected in accordance with the reaction sequence.

In particular, the technique of the applicant's Republic of Korea Patent Publication No. 10-2009-0101764 is to fill the fluid with a large dielectric constant in the reaction chamber before the reaction, during the reaction to remove the fluid having a large dielectric constant in the reaction chamber and the reaction solution and The process of filling the sample to be detected and the process of removing the reaction solution and refilling the fluid having a high dielectric constant after the completion of the reaction must be performed. There is an urgent need for the development of a device capable of injecting efficiently.

Therefore, there is a problem that the conventional method and the biosensor for injecting the reaction solution and liquid sample into the reaction vessel has to be improved.

Accordingly, the present invention is an object of the present invention to solve the problems of the conventional biosensor, specifically, the method of injecting the reaction solution and the liquid sample, the reaction target in the reaction vessel in which the biological, biochemical or chemical reaction is performed It is an object of the present invention to provide a device for selectively and sequentially injecting various liquids and a biosensor including the same.

Specifically, the present invention is to selectively and sequentially a plurality of liquids, for example, deionized water, reaction solution and the liquid sample to be tested in the reaction vessel in which the biochip embedded in the biosensor, such as DNA analysis device is equipped An object of the present invention is to provide a device for injecting and a biosensor including the same.

An apparatus for selectively injecting a liquid into the reaction vessel of an embodiment of the present invention for achieving the above object,

A plurality of inflow passages through which liquid is injected, a plurality of flow passages corresponding to the plurality of inflow passages, respectively, and connected to the reaction vessel;

A connecting passage formed through the inside of the housing along a longitudinal direction in a central axis spaced apart from the plurality of inflow passages by a predetermined distance and connected to the plurality of flow passages;

A connection groove inserted into the connection passage and rotatably coupled to the housing, the connection groove selecting and communicating with one of the plurality of flow paths in accordance with rotation about the central axis, and communicating with the connection groove and the reaction vessel. And a flow path selecting means having a discharge port for discharging the liquid introduced through the selected one flow path and the connection groove into the reaction vessel.

The apparatus for selectively injecting liquid into the reaction vessel of an embodiment of the present invention may further include a plurality of syringes each inserted into the plurality of inflow passages and containing the liquid. The liquid in the syringe flows through an injection hole formed in front of the syringe as the piston moves forward, and the injection hole communicates with the flow path formed in the housing. In addition, the plurality of inflow passages may be formed radially around the connection passage. For example, five inflow passages may be formed radially, and each inflow passage may form an angle of 72 ° with respect to an adjacent inflow passage. However, the present invention is not limited thereto, and various numbers of inflow passages may be applicable, and the angle may also be modified.

In the apparatus for selectively injecting liquid into the reaction vessel of an embodiment of the present invention, the flow path selection means is a cylindrical body portion of the rear open, the coupling groove is formed in the periphery while being coupled to the front of the body portion And the discharge port includes a head portion formed at the tip.

The device for selectively injecting liquid into the reaction vessel of an embodiment of the present invention further comprises a horizontal reciprocating motion device and a rotary motion device, the horizontal reciprocating motion device according to the operation of the horizontal reciprocating motion device and the rotary motion device And the rotating shaft and the piston shaft connected to the rotary motion device repeats the horizontal movement and rotation operation.

By the horizontal movement of the horizontal reciprocating motion device is inserted into the open rear of the body portion of the flow path selection means and the head portion of the flow path selection means that is interlocked with the rotation shaft by the rotation of the rotary motion device The connection groove formed in the connection groove is rotated by a predetermined angle to select one of the plurality of flow paths so that the connection groove and the selected flow path communicate with each other.

Meanwhile, the piston shaft is positioned in a syringe inserted into an inflow passage communicating with the selected flow path by the rotation of the predetermined angle, and the piston shaft moves the piston of the syringe by an additional horizontal movement of the horizontal reciprocating device. The pushing operation is performed to flow the liquid in the syringe through the inlet, and the flowing liquid is discharged into the reaction vessel from the outlet of the flow path selecting means through the selected flow path and the connecting groove.

Accordingly, the present invention can selectively and sequentially inject various liquids to be reacted into a reaction vessel in which a biological, biochemical or chemical reaction is performed by repeating the horizontal movement and rotation operations as described above. Will be.

In the apparatus for selectively injecting liquid into the reaction vessel of an embodiment of the present invention, the plurality of flow passages are preferably formed in a radially branched form from the connecting passage. The plurality of radially branched flow passages are configured to communicate with each of the plurality of inflow passages so that the liquid introduced from each inflow passage flows in. For example, five flow paths can be formed radially branched, and each flow path can form an angle of 72 ° with respect to an adjacent flow path. However, the present invention is not limited thereto, and various numbers of flow paths are applicable, and the angle thereof may also be modified.

In the device for selectively injecting liquid into the reaction vessel of an embodiment of the present invention, the housing can be bonded to the reaction vessel by ultrasonic welding to maintain the airtight therebetween. In addition, a coupling plate having a through hole through which liquid flows may be inserted between the housing and the reaction vessel.

In the device for selectively injecting liquid into the reaction vessel of an embodiment of the present invention, the reaction vessel is formed with an inlet for the liquid inlet and an outlet for the liquid outlet, the valve means for opening and closing the inlet and the outlet Can be provided. The valve means includes a protruding valve corresponding to the inlet and the outlet and formed of an elastic material. The protruding valve is pressed by pressure when the blocking shaft connected to the vertical reciprocating device descends to close the inlet and the outlet while being pressed by pressure, and when the blocking shaft is raised to release the pressure, the valve is in the original position by the elastic restoring force. Return to to open the inlet and the outlet.

In addition, the biosensor of the present invention is characterized by having a device for selectively injecting a liquid into the reaction vessel as described above.

According to the present invention having the above-described configuration, the present invention can selectively and sequentially inject several liquids to be reacted into a reaction vessel in which a biological, biochemical or chemical reaction is performed. Specifically, the present invention is to selectively and sequentially a plurality of liquids, for example, deionized water, reaction solution and the liquid sample to be tested in the reaction vessel in which the biochip embedded in the biosensor, such as DNA analysis device is equipped Can be injected by

Therefore, in the present invention, when it is necessary to inject various reaction solutions and the liquid sample to be tested into the reaction chamber in which the biochip is embedded according to the reaction sequence, the reaction solution and the liquid sample are exchanged before and after the reaction and manually injected into the reaction chamber. There is an advantage that can be efficiently injected in accordance with the reaction sequence to remove the inconvenience and must be in accordance with the reaction sequence.

1 is a schematic diagram of a biosensor equipped with a device for selectively injecting liquid into a reaction vessel according to one embodiment of the present invention.

2 is an exploded perspective view of an apparatus for selectively injecting a liquid into a reaction vessel according to an embodiment of the present invention, and a reaction vessel.

3 is a cross-sectional view in which a device for selectively injecting a liquid into a reaction vessel according to an embodiment of the present invention is combined with the reaction vessel.

4 to 10 is a state diagram of the use of the device for selectively injecting liquid into the reaction vessel according to an embodiment of the present invention.

11 is a view showing a device for selectively injecting a liquid into the reaction vessel according to an embodiment of the present invention in the direction A of FIG. 2, with a syringe and a flow path selecting means inserted therein.

12 is a view showing a device for selectively injecting a liquid into the reaction vessel according to an embodiment of the present invention in the direction B of Figure 2, a perspective view before the syringe and the flow path selection means is installed.

Hereinafter, exemplary embodiments of the present invention will be described with reference to the accompanying drawings. The following examples of the present invention are not intended to limit or limit the scope of the present invention only to embody the present invention. From the detailed description and examples of the present invention, those skilled in the art to which the present invention pertains can easily be interpreted as belonging to the scope of the present invention. References cited in the present invention are incorporated herein by reference.

As shown in FIG. 1, the biosensor 100 includes an apparatus 10 for selectively injecting a liquid into the reaction vessel of the present invention, and a reaction vessel 20 coupled to one side of the apparatus 10 of the present invention. The rotary motion device 30 and the horizontal reciprocating motion device 40 are included as main components. The rotary motion device 30 is driven by the rotary motor 36 to perform the rotary motion, and the horizontal reciprocating motion device 40 is driven by the linear motor 44 to perform horizontal reciprocating motion (ie, forward movement and backward movement). Guided by this horizontal reciprocating motion guide 42. According to the operation of the horizontal reciprocating motion device 40 and the rotary motion device 30 as described above, the rotary shaft 32 and the piston shaft 34 connected to the horizontal reciprocation motion device 40 and the rotary motion device 30 are connected. By repeating the horizontal movement and rotation operation together, various liquids M to be reacted to the reaction vessel 20 can be selectively and sequentially injected in the reaction order as will be described later.

Meanwhile, hereinafter, detailed descriptions of components that are known in the art constituting the biosensor 100 will be omitted for convenience of description. For example, the rotation direction, the rotation angle, etc. of the rotary motor 36 and the rotary motion device 30 may be controlled by a known sensor device such as a position sensor and a limit sensor and a controller connected thereto, and the linear motor 44 may be connected with the linear motor 44. The first forward movement, additional forward movement, backward movement, horizontal movement distance, etc. of the horizontal reciprocating motion device 40 is also controllable by a known sensor device such as a position sensor and a limit sensor and a control unit connected thereto. Those skilled in the art will readily understand.

2, 3, 11 and 12, the apparatus 10 for selectively injecting liquid into the reaction vessel of one embodiment of the present invention is largely coupled to one side of the reaction vessel 20 ( 11) and a flow path selecting means 12 inserted into the connecting passage 14 formed through the housing 11 along the longitudinal direction and rotatably coupled to the housing 11.

The housing 11 includes a plurality of inflow passages 15a, 15b, 15c, 15d, and 15e into which liquid is injected, and a plurality of inflow passages 15a, 15b, 15c, 15d, and 15e, respectively. The flow paths 146a, 146b, 146c, 146d, and 146e are provided (refer FIG. 11 and FIG. 12). In addition, a connection passage 14 is formed through the inside of the housing 11, and the connection passage 14 is a center spaced apart from a plurality of inflow passages 15a, 15b, 15c, 15d, and 15e by a predetermined distance. It is formed through the inside of the housing 11 along the longitudinal direction in the axis and is connected to the plurality of flow paths 146a, 146b, 146c, 146d, 146e (see FIGS. 2, 11 and 12).

The flow path selecting means 12 is inserted into the connection path 14 and rotatably coupled to the housing 11. Flow path selecting means 12 is one of a plurality of flow paths (146a, 146b, 146c, 146d, 146e) according to the rotation about the central axis of the housing 11 (see, for example, 146a: FIGS. 3 and 11). The liquid flowed through the connection groove 124 and the connection groove 124 and the connection groove 124 and the reaction vessel 20 and selectively flows through the selected flow path (146a) and the connection groove 124 to selectively communicate with the reaction vessel ( 20) an outlet 122 for discharging into the chamber is formed (see FIG. 3). In addition, the flow path selection means 12 is composed of a cylindrical body portion 126 of the rear open, and a head portion 120 coupled to the front of the body portion 126. At this time, the connection groove 124 is formed in the peripheral portion of the head 120 and the discharge port 122 is formed at the front end (see Fig. 2). In addition, an outlet packing member 128 is provided between the outlet 122 and the reaction vessel 20 to prevent the liquid from leaking when the liquid discharged from the outlet 122 enters the reaction vessel 20.

In addition, the device 10 for selectively injecting the liquid into the reaction vessel of one embodiment of the present invention is a plurality of syringes 16 are respectively inserted into the plurality of inflow passages (15a, 15b, 15c, 15d, 15e) and accommodated in the liquid ) (See FIGS. 2, 11 and 12). The plurality of syringes 16 is composed of a body portion (164a, 164b, 164c, 164d, 164e), injection holes (166a, 166b, 166c, 166d, 166e) and the locking jaw (168a, 168b, 168c, 168d, 168e) When the plurality of syringes 16 are inserted and installed corresponding to the plurality of inflow passages 15a, 15b, 15c, 15d, and 15e, respectively, the locking jaws 168a, 168b, 168c, 168d, and 168e of each syringe Engaged in the stepped portion of the inflow passage (15a, 15b, 15c, 15d, 15e) to prevent the syringe 16 from being separated from the housing 11 (see Figs. 2 and 3). In addition, packing members 162a, 162b, 162c, 162d, and 162e are inserted between the injection holes 166a, 166b, 166c, 166d, and 166e and the corresponding flow paths 146a, 146b, 146c, 146d, and 146e. It is possible to reliably prevent leakage of liquid flowing out of the inlet and flowing into the flow path.

The liquid in each syringe 16 flows through each inlet 166a, 166b, 166c, 166d, 166e formed in front of each syringe in accordance with the advancement of each piston 17 installed behind each syringe, Each inlet 166a, 166b, 166c, 166d, 166e is in communication with each corresponding flow path 146a, 146b, 146c, 146d, 146e formed in the housing 11 (see FIG. 11). The piston 17 is hermetically inserted into the syringe 16 to reciprocate. The piston body 172 is guided and moved by the inner surface of the syringe 16 and is hermetically inserted into the piston body 172. Consisting of a finish 174 to be joined (see FIGS. 2 and 3). The piston shaft 34, which will be described later, comes into contact with the finishing portion 174 of the piston 17 so that the piston 17 moves forward.

On the other hand, the plurality of inflow passage (15a, 15b, 15c, 15d, 15e) may be formed radially around the connection passage 14, preferably may be formed on a concentric circle. For example, as illustrated in FIG. 12, five inflow passages 15a, 15b, 15c, 15d, and 15e may be formed radially, and each inflow passage may have an angle of 72 ° with respect to an adjacent inflow passage. Can be formed. However, the present invention is not limited thereto, and various numbers of inflow passages may be applicable, and the angle may also be modified.

In addition, the plurality of flow paths (146a, 146b, 146c, 146d, 146e) is also preferably formed in a radially branched form from the connecting passage (14). Each of these radially branched flow paths 146a, 146b, 146c, 146d, 146e is in communication with each corresponding inflow path 15a, 15b, 15c, 15d, 15e to each inflow path 15a, 15b. , 15c, 15d, 15e are configured to flow in (see FIGS. 11 and 12). For example, as shown in FIG. 11, five flow paths 146a, 146b, 146c, 146d, 146e can be formed radially branched, with each flow path being 72 ° relative to an adjacent flow path. It can form the angle of. However, the present invention is not limited thereto, and various numbers of flow paths are applicable, and the angle thereof may also be modified.

As shown in Figures 2 and 3, the housing 11 can be bonded to one side of the reaction vessel 20 by ultrasonic welding to maintain the airtight therebetween. In addition, the coupling plate 25 having the through hole 252 is inserted and installed between the housing 11 and the reaction vessel 20 to facilitate the bonding of the housing 11 and the reaction vessel 20.

As shown in FIG. 1, the rotary motion device 30 is driven by the rotary motor 36 to perform the rotary motion, and the horizontal reciprocating motion device 40 is driven by the linear motor 44 to perform the horizontal reciprocating motion ( That is, forward movement and backward movement) are guided by the horizontal reciprocating motion guide 42. According to the operation of the horizontal reciprocating motion device 40 and the rotary motion device 30 as described above, the rotary shaft 32 and the piston shaft 34 connected to the horizontal reciprocation motion device 40 and the rotary motion device 30 are connected. The horizontal movement and rotation movements are repeated together.

Specifically, after the rotary shaft 32 is inserted into the open rear of the trunk portion 126 of the flow path selecting means 12 by the horizontal movement of the horizontal reciprocating motion device 40 driven by the linear motor 44. The connection groove 124 formed in the head portion 120 of the flow path selecting means 12 is rotated by a predetermined angle of the rotary motion device 30 driven by the rotation motor 36. For example, the connection groove 124 and the selected flow path 146a may be rotated by 72 ° to select one of the plurality of flow paths 146a, 146b, 146c, 146d, and 146e, for example, 146a. Communicating. As such, a state in which the connecting groove and one selected flow path communicate with each other is illustrated in FIG. 11. It can be seen that the connection groove 124 and the selected flow path 146a communicate with each other at position ① of FIG. 11. On the other hand, the piston shaft 32 connected to the rotary motion device 30 by a predetermined angle (for example, 72 °) of the rotary motion device 30 as described above is connected to the selected flow path (146a) and The piston shaft 34 is positioned on the rear side of the syringe body portion 164a inserted into the inflow passage 15a communicating therewith and by the horizontal movement of the horizontal reciprocating motion device 40 driven by the linear motor 44. Pushing the finish portion 174 of the piston 17, which is inserted into the back of the syringe barrel 164a, flows the liquid in the syringe through the inlet 166a, and the spilled liquid is selected flow path 146a. And into the reaction vessel 20 from the outlet 122 of the flow path selection means 12 through the coupling groove 124. The flow path of the liquid flowing in this way is indicated by the symbol "F" in FIG. 3.

Accordingly, the present invention can selectively and sequentially inject various liquids to be reacted into the reaction vessel 20 in the reaction order as the horizontal movement and rotation operations as described above are repeated. For example, when the rotary motion device 30 is rotated in the clockwise direction by the driving of the rotary motor 36, the rotating shaft 32 and the linked shaft 32 in the order of ① → ② → ③ → ④ → ⑤ of FIG. The flow path selecting means 12 is rotated so that the connecting groove 124 sequentially selects the flow path (146a → 146b → 146c → 146d → 146e), and the piston shaft 34 is also interlocked and rotates clockwise. After that, it is possible to sequentially inject several liquids to be reacted by horizontal reciprocating movement. On the other hand, when the rotary motion device 30 is rotated in the counterclockwise direction by the drive of the rotary motor 36, the rotating shaft 32 and the interlocking movement with the rotary shaft 32 in the order of ① → ⑤ → ④ → ③ → ② of FIG. The flow path selecting means 12 is rotated so that the connecting groove 124 selects the flow path in the reverse order (146a → 146e → 146d → 146c → 146b), and the piston shaft 34 is also interlocked in the counterclockwise direction. After rotation, the horizontal reciprocating movement allows the various liquids to be reacted to be injected in the reverse order.

Next, as shown in FIG. 2, the reaction vessel 20 into which the liquid is injected is largely configured by combining the upper case 22, the valve means 24, the case body 26, and the lower case 21. . The chip cover support plate 272 is inserted and coupled between the case body 26 and the periphery of the lower case 21, and the chip cover 271 formed to be stepped downward from the chip cover support plate 272 is provided with a biochip 28. It is positioned on the biochip 28 at a minute spacing S (see FIG. 4).

The case body 26 of the reaction vessel 20 has an inlet 26a through which liquid flows in and an outlet 26b through which liquid flows out. Correspondingly, a chip cover inlet 27a and a chip also exist in the chip cover 271. A lid outlet 27b is formed. The liquid introduced into the reaction vessel 20 passes through the inlet 26a and the chip cover inlet 27a to penetrate into the microcavity between the chip cover 271 and the biochip 28.

When liquid enters the biochip 28, it is necessary to block the inlet 26a and the outlet 26b so that biological, biochemical or chemical reactions on the biochip 28 can be stably performed. For this purpose, a valve means 24 for opening and closing the inlet 26a and the outlet 26b is provided on the case body 26. The valve means 24 includes protruding valves 24a and 24b which correspond to the inlet 26a and the outlet 26b and are made of an elastic material, for example, silicon. The protruding valves 24a and 24b close the inlet 26a and the outlet 26b while being pressed by the pressure when the blocking shafts 52 and 56 connected to the vertical reciprocating device 50 descend and apply pressure. When the blocking shafts 52 and 56 rise and the pressure is released, the inlet 26a and the outlet 26b are opened by the elastic restoring force (see FIGS. 2 and 4 to 10).

On the other hand, the components constituting the device 10 for selectively injecting liquid into the reaction vessel of the present invention is preferably composed of a synthetic resin, for example, may be composed of polyethylene resin. However, the present invention is not limited thereto, and various materials known in the art may be applied.

The operation of the apparatus 10 for selectively injecting liquid into the reaction vessel of the embodiment of the present invention configured as described above will be described with reference to FIGS. 4 to 10. In FIG. 5 to FIG. 10, a repetitive description of reference numerals is omitted, and some of the reference numerals are omitted for convenience of illustration. Reference numerals omitted in FIGS. 5 to 10 refer to FIG. 4.

(A) Preparation step before selecting the flow path: see FIGS. 4 and 5

In FIG. 4, the rotation shaft 32 and the piston shaft 34 are just before the horizontal movement and rotation operation are performed, and the vertical reciprocating motion device 50 is a reaction vessel 20 for vertical movement of a short vertical movement distance. It is moving near and waiting. The shock absorbing springs 54 and 58 of the vertical reciprocating device 50 absorb vibrations or minute shocks applied to the vertical reciprocating device 50 to prevent unwanted movement of the blocking shafts 52 and 56. At this time, the connecting groove 124 formed in the head portion 120 of the flow path selecting means 12 is directed to the position ⑤ of FIG. Therefore, the flow path 146a is not in communication with the connecting groove 124 and is blocked by the head portion 120 of the flow path selecting means 12.

In FIG. 5, the rotation shaft 32 is hermetically inserted into the open rear of the trunk portion 126 of the flow path selecting means 12 by the horizontal movement of the horizontal reciprocating motion device 40 driven by the linear motor 44. And the piston shaft 34 moves forward in conjunction with the horizontal movement of the horizontal reciprocating motion device 40, but is located close to the rear of the syringe barrel portion 164e installed in the inflow passage 15e. (Corresponding to position ⑤ in FIG. 11) The axial direction is inconsistent with the syringe body portion 164a installed in the inflow passage 15a.

In the next operation, the rotary shaft 32 is rotated by a clockwise rotation (indicated by "C" in the drawing) of a predetermined angle (for example, 72 °) of the rotary motion device 30 by driving the rotary motor 36. And the piston shaft 34 rotate together, and the flow path selecting means 12 in which the rotary shaft 32 is hermetically inserted also rotates along the rotary shaft 32.

(B) Step ① flow path 146a selection step: see FIG.

As described above, the connecting groove 124 formed in the head portion 120 of the flow path selecting means 12 also rotates according to the clockwise rotation of the flow path selecting means 12. Is rotated by. At this time, the connecting groove 124 is directed to position ① of FIG. Therefore, the connecting groove 124 is selectively in communication with the flow path 146a and is also in communication with the inlet 166a of the syringe.

And, by the clockwise rotation as described above, the piston shaft 32 is located close to the rear of the syringe body portion 164a installed in the inlet passage 15a communicating with the selected flow passage 146a, and the syringe barrel The portion 164a is in a state where the axial direction coincides.

In the next operation, the rotary shaft 32 and the piston shaft 34 are further advanced in the D direction by the additional horizontal movement of the horizontal reciprocating motion device 40 driven by the linear motor 44.

(C) liquid injection into the reaction vessel: see FIGS. 7 and 8

In FIG. 7, the rotating shaft 32 advances to an extra space in the trunk portion 126 of the flow path selecting means 12 in accordance with the additional forward movement of the rotating shaft 32 and the piston shaft 34 as described above. It is a state fully inserted in the longitudinal direction of 126. In addition, the piston shaft 34 pushes the finish portion 174 of the piston 17, which is inserted into the rear surface of the syringe barrel portion 164a, to push the liquid M in the syringe through the inlet 166a. The discharged liquid M is discharged through the flow passage 146a selected in step (B) and the connection groove 124 communicating with the selected flow passage 146a. ) Into the reaction vessel 20.

The liquid M introduced into the reaction vessel 20 through the flow path of the liquid denoted by "F" in FIG. 7 passes through the inlet 26a and the chip cover inlet 27a, and the chip cover 271 and the biochip. It penetrates into the microcavity between 28. The liquid M is filled in the space 28b between the electrode forming portions 28a of the biochip 28 and is ready to perform a biological, biochemical or chemical reaction on the biochip 28.

Then, the rotary shaft 32 and the piston shaft 34 retreat in the D 'direction by the horizontal movement in the reverse direction of the horizontal reciprocating motion device 40 driven by the linear motor 44. At this time, the retreat distance in the D 'direction is the same as the advance distance in the D direction. In FIG. 8, the rotary shaft 32 and the piston shaft 34 are retracted, so that the piston shaft 34 exits from the syringe body portion 164a and is located close to the rear surface of the syringe body portion 164a, and the rotary shaft 32 is only partially. The state inserted in the trunk portion 126 of this flow passage selecting means 12 is shown.

In the next operation, the blocking shafts 52 and 56 connected to the vertical reciprocating motion device 50 descend in the E direction.

(D) Inlet and outlet blocking steps of the reaction vessel: see FIG. 9

In FIG. 9, the valve means 24 blocks the inlet 26a and the outlet 26b. That is, as described above, when the blocking shafts 52 and 56 connected to the vertical reciprocating motion device 50 descend and apply pressure to the protruding valves 24a and 24b made of an elastic material, the protruding valves 24a and 24b It is pressed downward by the applied pressure and closes the inlet 26a and the outlet 26b while contacting the inlet 26a and the outlet 26b.

As the inlet 26a and the outlet 26b of the reaction vessel 20 are closed, foreign matter other than the liquid M introduced onto the biochip 28 cannot be introduced into the reaction vessel 20, so that the biochip Biological, biochemical or chemical reactions on (28) can be carried out stably.

When the first reaction in the reaction vessel 20 is completed, as the next operation, the blocking shafts 52 and 56 connected to the vertical reciprocating motion device 50 rise in the E 'direction. At this time, the rising distance in the E 'direction is the same as the falling distance in the E direction. Then, the rotary shaft 32 and the clockwise rotation (indicated by "C" in the figure) of the predetermined angle (for example, 72 °) of the rotary motion device 30 by the drive of the additional rotary motor 36 and The flow path selection means 12 in which the piston shaft 34 rotates together and the rotary shaft 32 is hermetically inserted also rotates along the rotary shaft 32.

(E) Selecting step 2 flow path 146b step: see FIG.

In FIG. 10, the connecting groove 124 faces the position ② of FIG. 11. Therefore, the connection groove 124 is selectively in communication with the flow path 146b and also in communication with the inlet 166b of the syringe. Further, by the additional clockwise rotation as described above, the piston shaft 32 is positioned in close proximity to the rear of the syringe body portion 164b installed in the inflow passage 15b communicating with the selected flow passage 146b. The trunk portion 164b is in a state where the axial direction coincides.

Then, the steps (C) and (D) are repeated. Through this repeating process, the rotating shaft 32 and the flow path selecting means 12 linked thereto rotate in the order of ② → ③ → ④ → ⑤ of FIG. 11 so that the connecting groove 124 sequentially selects the flow path. (146b → 146c → 146d → 146e), the piston shaft 34 also rotates in conjunction with the horizontal reciprocating movement to be able to inject several liquids to be reacted sequentially.

Although the present invention has been described with reference to the above embodiments, the present invention is not limited thereto. Those skilled in the art can make modifications and changes without departing from the spirit and scope of the present invention, and it will be appreciated that such modifications and changes also belong to the present invention.

Claims (10)

1. An apparatus for selectively injecting liquid into a reaction vessel,

   A plurality of inflow passages through which liquid is injected, a plurality of flow passages corresponding to the plurality of inflow passages, respectively, and connected to the reaction vessel;

   A connecting passage formed through the inside of the housing along a longitudinal direction in a central axis spaced apart from the plurality of inflow passages by a predetermined distance and connected to the plurality of flow passages;

   A connection groove inserted into the connection passage to be rotatably coupled to the housing, the connection groove being selected to communicate with one of the plurality of flow paths in accordance with rotation about the central axis, and communicating with the connection groove and the reaction vessel. And a flow path selection means having a discharge port configured to discharge the liquid introduced through the selected one flow path and the connecting groove into the reaction vessel.
2. The method of claim 1,

   And a plurality of syringes each inserted into the plurality of inflow passages and containing a liquid therein, wherein the liquid is selectively injected into the reaction vessel.
3. The method of claim 2,

   The liquid in the syringe flows through an injection hole formed in front of the syringe in accordance with the advancement of the piston installed behind the syringe, the injection hole is a device for selectively injecting liquid into the reaction vessel, characterized in that in communication with the flow path formed in the housing .
4. The method according to any one of claims 1 to 3,

   The plurality of inflow passages are formed radially around the connection passage, and the plurality of flow passages are formed radially branched from the connection passages, and the plurality of radially branched flow passages are the plurality of inflow passages. A device for selectively injecting liquid into the reaction vessel, each in communication with the passage.
5. The method of claim 3,

   The flow path selecting means includes a body portion of a cylindrical shape with a rear opening, and a head portion coupled to the front of the body portion, the connecting groove being formed at the periphery portion and the outlet portion being at the tip portion. Injecting device.
6. The method of claim 5,

   Further comprising a horizontal reciprocating motion device and a rotary motion device, the horizontal reciprocating motion device and the rotary shaft and the piston shaft connected to the horizontal reciprocating motion device and the rotary motion device in accordance with the operation of the horizontal reciprocating motion device and the rotational motion device together A device for selectively injecting liquid into the reaction vessel, characterized in that performing.
7. The method of claim 6,

   By the horizontal movement of the horizontal reciprocating motion device is inserted into the open rear of the body portion of the flow path selection means, the rotating shaft and the head portion of the flow path selection means interlocked with the rotation motion device by the rotation And the connection groove is in communication with the selected flow path by selecting one of the plurality of flow paths by rotating the connection groove formed by a predetermined angle.
8. The method of claim 7, wherein

   The piston shaft is positioned in a syringe inserted into an inflow passage communicating with the selected flow path by the rotation of the predetermined angle, and the piston shaft pushes the piston of the syringe by an additional horizontal movement of the horizontal reciprocating device. Liquid into the reaction vessel by flowing the liquid in the syringe through the inlet and discharged from the outlet of the flow path selection means to the reaction vessel through the selected flow path and the connecting groove. Device for selectively injecting.
9. The method according to any one of claims 1 to 3,

   The reaction vessel includes an inlet through which liquid flows in and an outlet through which liquid flows out, and includes a protruding valve corresponding to the inlet and the outlet and formed of an elastic material. When the valve is pressed by the pressure to close the inlet and the outlet, when the pressure applied to the protruding valve is released to return to the original position by the elastic restoring force of the protruding valve to open the inlet and the outlet A device for selectively injecting liquid into a reaction vessel characterized in that.
10. A biosensor comprising a device for selectively injecting liquid into a reaction vessel as defined in claim 1.

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