WO2012123712A1 - Cell-based screening assay - Google Patents
Cell-based screening assay Download PDFInfo
- Publication number
- WO2012123712A1 WO2012123712A1 PCT/GB2012/050463 GB2012050463W WO2012123712A1 WO 2012123712 A1 WO2012123712 A1 WO 2012123712A1 GB 2012050463 W GB2012050463 W GB 2012050463W WO 2012123712 A1 WO2012123712 A1 WO 2012123712A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cell
- cell culture
- epithelial cells
- lung
- hepatocytes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5067—Liver cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/27—Lung cells, respiratory tract cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/04—Screening or testing on artificial tissues
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Analytical Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1316076.7A GB2502491A (en) | 2011-03-17 | 2012-03-01 | Cell-based screening assay |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1104511.9 | 2011-03-17 | ||
GB201104511A GB201104511D0 (en) | 2011-03-17 | 2011-03-17 | Cell based screening assay |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2012123712A1 true WO2012123712A1 (en) | 2012-09-20 |
Family
ID=44012726
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2012/050463 WO2012123712A1 (en) | 2011-03-17 | 2012-03-01 | Cell-based screening assay |
Country Status (2)
Country | Link |
---|---|
GB (2) | GB201104511D0 (en) |
WO (1) | WO2012123712A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014062505A1 (en) * | 2012-10-18 | 2014-04-24 | The University Of Kansas | Improved reliability of assays using a multi-divot platform and multi-source, multi-cell type clusters |
WO2014151456A2 (en) * | 2013-03-15 | 2014-09-25 | The Jackson Laboratory | Treatment of inflammatory diseases |
CN107121553A (en) * | 2017-03-16 | 2017-09-01 | 国家烟草质量监督检验中心 | A kind of gas-liquid interface exposure system combines high intension technology and quantitatively detects the method that 1,3 butadiene cause DNA Damage |
EP3287521A1 (en) * | 2016-08-25 | 2018-02-28 | Philip Morris Products S.A. | Cell culture |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3127198A (en) | 1964-03-31 | figure | ||
US5989835A (en) | 1997-02-27 | 1999-11-23 | Cellomics, Inc. | System for cell-based screening |
WO2002048318A1 (en) | 2000-12-15 | 2002-06-20 | The University Of Nottingham | Co-culture of hepatocytes and stellate cells |
WO2009024748A1 (en) | 2007-08-17 | 2009-02-26 | Reinnervate Limited | Stem cell derived neurotrophic factors |
WO2010042669A2 (en) | 2008-10-07 | 2010-04-15 | Ipierian, Inc. | Co-culture compositions and methods |
WO2010118352A1 (en) | 2009-04-09 | 2010-10-14 | Arizona Board Of Reagents On Behalf Of The University Of Arizona | Cellular seeding and co-culture of a three dimensional fibroblast construct |
-
2011
- 2011-03-17 GB GB201104511A patent/GB201104511D0/en not_active Ceased
-
2012
- 2012-03-01 WO PCT/GB2012/050463 patent/WO2012123712A1/en active Application Filing
- 2012-03-01 GB GB1316076.7A patent/GB2502491A/en not_active Withdrawn
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3127198A (en) | 1964-03-31 | figure | ||
US5989835A (en) | 1997-02-27 | 1999-11-23 | Cellomics, Inc. | System for cell-based screening |
WO2002048318A1 (en) | 2000-12-15 | 2002-06-20 | The University Of Nottingham | Co-culture of hepatocytes and stellate cells |
WO2009024748A1 (en) | 2007-08-17 | 2009-02-26 | Reinnervate Limited | Stem cell derived neurotrophic factors |
WO2010042669A2 (en) | 2008-10-07 | 2010-04-15 | Ipierian, Inc. | Co-culture compositions and methods |
WO2010118352A1 (en) | 2009-04-09 | 2010-10-14 | Arizona Board Of Reagents On Behalf Of The University Of Arizona | Cellular seeding and co-culture of a three dimensional fibroblast construct |
Non-Patent Citations (9)
Title |
---|
BERUBE K ET AL: "Human primary bronchial lung cell constructs: The new respiratory models", 30 December 2010, TOXICOLOGY, LIMERICK, IR, PAGE(S) 311 - 318, ISSN: 0300-483X, XP027539132 * |
BÉRUBÉ KELLY ET AL: "Filter-well technology for advanced three-dimensional cell culture: perspectives for respiratory research.", ALTERNATIVES TO LABORATORY ANIMALS : ATLA DEC 2010 LNKD- PUBMED:21275484, vol. 38 Suppl 1, December 2010 (2010-12-01), pages 49 - 65, XP002677013, ISSN: 0261-1929 * |
BÉRUBÉ KELLY ET AL: "In vitro models of inhalation toxicity and disease. The report of a FRAME workshop.", ALTERNATIVES TO LABORATORY ANIMALS : ATLA FEB 2009 LNKD- PUBMED:19292579, vol. 37, no. 1, February 2009 (2009-02-01), pages 89 - 141, XP002677010, ISSN: 0261-1929 * |
CHOI SUE ET AL: "Feasibility of a simple double-layered coculture system incorporating metabolic processes of the intestine and liver tissue: application to the analysis of benzo[a]pyrene toxicity.", TOXICOLOGY IN VITRO : AN INTERNATIONAL JOURNAL PUBLISHED IN ASSOCIATION WITH BIBRA JUN 2004 LNKD- PUBMED:15046787, vol. 18, no. 3, June 2004 (2004-06-01), pages 393 - 402, XP002677009, ISSN: 0887-2333 * |
KELLX BERUBE: "trends in integrated testing strategies for in vitro extrapolation in chemical and drug safety for inhalation toxicology", BIOTECH INTERNATIONAL- BTI, vol. 22, September 2011 (2011-09-01), pages 10 - 14, XP002677014 * |
LI ALBERT P: "The use of the Integrated Discrete Multiple Organ Co-culture (IdMOC) system for the evaluation of multiple organ toxicity.", ALTERNATIVES TO LABORATORY ANIMALS : ATLA SEP 2009 LNKD- PUBMED:19807210, vol. 37, no. 4, September 2009 (2009-09-01), pages 377 - 385, XP002677012, ISSN: 0261-1929 * |
SANDRA SZAMEIT, LETIZIA FARMER, CHRISTINE HÖPFNER, MADHURI SINGAL, LEAH JONES, LADD SMITH, ELISABETH WEBER: "Development of a human lung model system for the identification of chemical respiratory allergens in vitro", 1 September 2009 (2009-09-01), XP002677011, Retrieved from the Internet <URL:http://www.frame.org.uk/dynamic_files/inhalation_toxicity_workshop_final.pdf> [retrieved on 20120601] * |
SEBASTIAN G KLEIN ET AL: "Potential of coculturemodels to study inflammatory and sensitizing effects of particles on the lung", TOXICOLOGY IN VITRO, ELSEVIER SCIENCE, GB, vol. 25, no. 8, 6 September 2011 (2011-09-06), pages 1516 - 1534, XP028110711, ISSN: 0887-2333, [retrieved on 20110919], DOI: 10.1016/J.TIV.2011.09.006 * |
TAYLOR ET AL., AM. SCIENTIST, vol. 80, 1992, pages 322 - 335 |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9567570B2 (en) | 2012-10-18 | 2017-02-14 | The University Of Kansas | Assays using a multi-divot platform and multi-source, multi-cell type clusters |
WO2014062505A1 (en) * | 2012-10-18 | 2014-04-24 | The University Of Kansas | Improved reliability of assays using a multi-divot platform and multi-source, multi-cell type clusters |
US10155928B2 (en) | 2012-10-18 | 2018-12-18 | The University Of Kansas | Assays using a multi-divot platform and multi-source, multi-cell type clusters |
US9964535B2 (en) | 2013-03-15 | 2018-05-08 | The Jackson Laboratory | Treatment of inflammatory diseases |
WO2014151456A2 (en) * | 2013-03-15 | 2014-09-25 | The Jackson Laboratory | Treatment of inflammatory diseases |
WO2014151456A3 (en) * | 2013-03-15 | 2014-12-18 | The Jackson Laboratory | Treatment of inflammatory diseases |
JP2021048865A (en) * | 2016-08-25 | 2021-04-01 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | Cell culture |
WO2018036910A1 (en) * | 2016-08-25 | 2018-03-01 | Philip Morris Products S.A. | Cell culture |
CN108138135A (en) * | 2016-08-25 | 2018-06-08 | 菲利普莫里斯生产公司 | Cell culture |
EP3287521A1 (en) * | 2016-08-25 | 2018-02-28 | Philip Morris Products S.A. | Cell culture |
JP2019506134A (en) * | 2016-08-25 | 2019-03-07 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | Cell culture |
US11041145B2 (en) | 2016-08-25 | 2021-06-22 | Philip Morris Products S.A. | Cell culture |
EP3940059A1 (en) * | 2016-08-25 | 2022-01-19 | Philip Morris Products S.A. | Cell culture |
JP7097942B2 (en) | 2016-08-25 | 2022-07-08 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | Cell culture |
CN107121553B (en) * | 2017-03-16 | 2019-08-27 | 国家烟草质量监督检验中心 | A kind of liquid-vapor interface exposure system combines the method for high intension technology quantitative detection 1,3- butadiene cause DNA Damage |
CN107121553A (en) * | 2017-03-16 | 2017-09-01 | 国家烟草质量监督检验中心 | A kind of gas-liquid interface exposure system combines high intension technology and quantitatively detects the method that 1,3 butadiene cause DNA Damage |
Also Published As
Publication number | Publication date |
---|---|
GB2502491A (en) | 2013-11-27 |
GB201316076D0 (en) | 2013-10-23 |
GB201104511D0 (en) | 2011-05-04 |
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