WO2010137884A2

WO2010137884A2 - Centrifugal separation method, centrifugal separation apparatus, and bag for centrifugal separation

Info

Publication number: WO2010137884A2
Application number: PCT/KR2010/003352
Authority: WO
Inventors: 정광회; 이광형; 최행석; 신준호; 고정재
Original assignee: (주)차바이오메드
Priority date: 2009-05-27
Filing date: 2010-05-27
Publication date: 2010-12-02
Also published as: WO2010137884A3

Abstract

The invention relates to a centrifugal separation method for separating a target substance from a sample in a separation bag, using said separation bag which has a centrifugal separation space for accommodating the sample and an outlet formed at the bottom of the centrifugal separation space to discharge the sample to the outside, the method comprising: a step of performing a centrifugal separation on the sample; a step of dividing the centrifugal separation space; and a step of discharging the target substance from the divided space adjacent to the outlet, while maintaining the divided state of the divided space.

Description

Centrifugal Separation Methods, Centrifugal Separators, and Centrifugal Bags

The present invention relates to a centrifugation method, a centrifugal separator, and a centrifugal bag for separating a target substance from a target material for centrifugation using centrifugation.

Centrifugation can be used to collect specific target materials for use from samples taken from the body of an animal or human.

In particular, the blood can be used for various medical purposes, such as transfusion of surgical patients, clinical trials of patients, and as a raw material for various medical drugs. The blood may be used as it is, but specific target substances may be collected and used from the blood. For example, platelets (blood platelet) is a type of blood cell that is a tangible component of the blood, is involved in blood coagulation or hemostatic action, and is easily used during surgery.

On the other hand, in order to collect the desired target substance from the blood, a centrifugation method is generally used. For centrifugation, the blood is centrifuged in a centrifugal separator in a state in which a container for centrifugation is placed. The blood may be a test tube or petri dish having a certain shape, but may be contained in a flexible centrifuge bag and centrifuged.

Centrifugal bags generally include an inlet formed at the top and an outlet formed at the bottom. However, when blood is accommodated in a centrifuge bag made of a flexible material and centrifuged, when a high pressure is applied to the centrifuge bag, a substance may leak out of the centrifuge bag through the outlet.

In addition, the swing-out centrifugal separator includes a rotating bucket coupled to a rotating shaft rotating at a high speed, and may include an adapter inserted into the rotating bucket while the centrifugal bag is fixed. have. However, the centrifugal bag made of a flexible material may be crushed in the rotating bucket during centrifugation to cause the material inside the centrifuge bag to be mixed during centrifugation.

In addition, in the conventional centrifugal separator, after centrifugation, the centrifugal bag must be separated from the adapter to discharge the target material collected at the bottom of the centrifugal bag. The centrifugal bag is separated in the process of separating the centrifugal bag from the adapter. It may happen that the materials inside are mixed.

The present invention is a centrifugal separation method, a centrifugal separator, which can prevent the surrounding of the discharge port of the centrifuge bag from being damaged by high pressure or leak the target material through the discharge port in the process of obtaining the target material from the target material through centrifugation. Provided is a centrifuge bag.

The present invention provides a centrifugal separation method, a centrifugal separation device, and a centrifugal separation bag which can prevent the centrifugal bag from being distorted during the centrifugation process to prevent the material inside the centrifugal bag from being mixed.

The present invention provides a centrifugal separation method, a centrifugal separation device, and a centrifugal separation bag capable of immediately discharging a desired substance without the process of separating the centrifugal bag from the adapter after centrifugation. In particular, the present invention provides a centrifugal separation method, a centrifugal separator, and a centrifugal bag capable of preventing the material inside the centrifugal bag from being mixed in the process of separating the centrifugal bag from the adapter after centrifugation.

According to an exemplary embodiment of the present invention for achieving the above object of the present invention, there is provided a separation bag provided with a centrifugation space for receiving the target material and an outlet for discharging the target material to the bottom of the centrifuge space. The centrifugation method for separating the target material from the target material in the separation bag includes the steps of: centrifuging the target material, dividing the centrifugation space, and maintaining the divided state. Draining the desired material from the space.

In the present specification, the target material may include a mixture of reactant reacting with the cell tissue as well as pure cell tissue extracted from the organism, and the reactant may be a desired object from the cell tissue described above. May contain enzymes or chemicals to isolate or extract cells.

In addition, the centrifugal separation method according to the present invention after the step of centrifuging the target material, by dividing the centrifugation space, maintaining the divided state, to discharge the target material from the divided space adjacent to the outlet side do. Thus, in a state in which the centrifugation space is divided, the target substance can be discharged from the outlet, and only the target substance can be easily collected through the outlet.

The division of the centrifugal space may be implemented by externally pressing the front and rear portions of the separation bag, and the discharge of the target substance may be performed in a state in which the centrifugal space and the outlet are physically separated. Similarly, the process of physically separating the centrifugal space and the discharge port may be implemented by externally pressing the front and rear portions of the separation bag. By centrifuging the target material in a state where the centrifugal space and the outlet are physically separated, it is possible to prevent the surrounding of the outlet of the centrifugal bag containing the target material from being damaged by high pressure or leaking of the target material through the outlet.

More specifically, when the adapter body corresponding to the rotating bucket for centrifugation is used, the centrifugal space and the outlet are physically separated using the first pressing member mounted to the adapter body, while the rotating bucket rotates. The target material can be prevented from leaking from the outlet, the second pressurizing member mounted on the adapter body can be used to divide the centrifuge space, and the mixing with other materials can be prevented during the discharge of the target material after centrifugation. have.

In some cases, the centrifugal space may be physically separated from the outlet by using the first pressing member, and after the centrifugal separation, the centrifugal space may be divided by moving the first pressing member. In this case, the first pressing member may naturally release the state in which the centrifugal space and the outlet are physically separated. For reference, the first pressing member may be provided to slide and fix up and down along the adapter body.

According to another exemplary embodiment of the present invention for achieving the above object of the present invention, there is provided a centrifugation space for accommodating the target material of the centrifugation and an outlet for discharging the target material to the outside at the bottom of the centrifugation space. A centrifugal separator for separating a target substance from a target material in the separating bag, using a separating bag, provides a bag receiving space therein to receive the separating bag, and is equipped with an adapter body for fixing the separating bag, and an adapter body. It includes a rotating bucket, it is possible to centrifuge the target material by rotating the rotating bucket.

In addition, it may further include a pressing member for physically separating the centrifugal space. The pressurizing member includes a first pressurizing member that pressurizes the front and rear portions of the separating bag from the outside to physically separate the centrifugal space and the outlet, and a second pressurizing member which pressurizes the front and back portion of the separating bag from the outside to divide the centrifugal space. can do.

In addition, the pressure member may pressurize the region between the centrifuge space and the outlet to physically separate the centrifuge space and the outlet, and in some cases, the pressure member selectively separates the region between the target material and the separation material after centrifugation. You can also

The pressing member can be fixed to the adapter body in a different position up and down, is fixed in a position to prevent leakage from the discharge port while the rotating bucket is rotated, and prevents mixing with other materials while discharging the target substance after centrifugation It can be fixed in position to physically separate the separation bag. In such a case, the pressing member may be provided to be able to release, slide, and fix the fixed state with respect to the adapter body.

The adapter body may specifically include an upper adapter body member and a lower adapter body member that overlap each other to form a bag receiving space therein, wherein the upper adapter body member may selectively expose the lower adapter body member to expose the bag receiving space. For example, the upper adapter body member may be hinged laterally with the lower adapter body member.

In addition, the lower adapter body member is formed with a body binding portion, the pressing member may include a pressing bar provided in the form of a bar, and the pressing member binding portion that is bound to the body binding portion to bind the pressing bar to the lower adapter body member.

In addition, the upper adapter body member may include an exposure ball for exposing the bag receiving space, in this case, the pressure bar may be bindable to the lower adapter body through the exposure ball.

In addition, the lower adapter body member may include a support bar that protrudes from the inner surface of the lower adapter body and supports the separation bag pressed by the pressure bar at the bottom.

In addition, the bottom of the adapter body may be formed to be recessed around the outlet of the separating bag.

In addition, the upper fixing part and the lower fixing part may be formed inside the upper adapter body member and the lower adapter body member to be coupled to each other to respectively press the edges of the separating bag to fix the separating bag.

In addition, the adapter body has a plurality of vertical incision body each comprising a horizontal movement preventing portion in contact with the inner surface of the rotating bucket, and a vertical support extending in the longitudinal direction of the rotating bucket integrally with the horizontal movement preventing portion, vertical incision When the bodies are engaged with each other, the horizontal movement preventing portion at least partially touches the inner surface of the rotating bucket so as to prevent horizontal movement inside the rotating bucket, and the vertical support portion closely adheres to both sides of the separating bag so that the rotating bucket can be rotated during the centrifugation process. The separation bag can be fixed in place.

Here, the vertical incision body may be formed in a frame of a frame shape symmetrical with each other, the horizontal movement preventing portion, corresponding to the horizontal frame in which both ends contact the inner surface of the rotating bucket at a predetermined interval in the longitudinal direction of the rotating bucket The vertical support may correspond to a vertical frame extending in the longitudinal direction of the rotating bucket so as to connect the horizontal frames.

For reference, the vertical incision body may include an upper adapter body member and a lower adapter body member as the adapter body.

According to another exemplary embodiment of the present invention for achieving the above object of the present invention, the centrifugal bag used in the centrifugal separator is formed by fusing at least one synthetic resin film formed by using a synthetic resin, the top A bag body provided at an inlet, an upper separation space in communication with the inlet, a lower separation space having a narrower width than the upper separation space, and a discharge opening for discharging the target material collected in the lower separation space; A fusion formed around the body of the bag by fusion of the synthetic resin film and formed along the shape of the adapter body to be fixed by the adapter body; And a pressing member mounting hole formed on both sides of the lower separation space to allow the pressing member to be mounted.

The fusion portion is fixed to the adapter body so that it is not easily distorted at the time of centrifugation, and the bottom of the upper separation space and the sides of the lower separation space may have a narrow structure toward the outlet. Through this structure, a jaw does not occur between the upper separation space and the lower separation space, and the accumulation of the target material on the jaw can be effectively prevented.

In addition, the side of the lower separation space may have a structure that narrows and forms an inclined angle that is relatively sharper than the bottom of the upper separation space.

The bottom of the upper separation space is preferably formed to form an inclination angle of 15 to 85 degrees with respect to the horizontal.

In addition, one or two or more of the pressing member mounting holes may be symmetrically formed on both sides of the lower separation space. When forming two or more pressing member mounting holes, it is possible to form a bridge fusion portion which is connected to the outermost fusion portion along the edge of the fusion portion to the side of the lower separation space, and the bridge fusion portion formed between the mounting holes is lower in the separation bag body. It can serve as a support structure for supporting the sides of the separation space.

The fusion portion of the pressure member mounting hole adjacent to the discharge port can be formed in a relatively wider width than the periphery of other pressure member mounting holes, and in this case, when fixed by the upper and lower adapter body members, it can be stably fixed.

For reference, the centrifugal bag is preferably formed using a synthetic resin so that its shape can be flexibly changed when the target material is introduced into the centrifuge space therein. For example, synthetic resins may use polyvinyl chloride or polymers of dimethylsiloxane and polycarbonate. In particular, synthetic resins, such as polyvinyl chloride, have no absorption but elasticity and resistance to chemicals. Other materials may be used.

The bag body may be formed by fusing at least one sheet of synthetic resin film formed using the above-described synthetic resin, and sealing the edges of the separating bag except the inlet and the outlet by welding.

By using a relatively low cost synthetic resin, it is possible to reduce the manufacturing cost of the centrifugal bag, and thus it is possible to use the centrifugal bag for single use. In addition, it is possible to extract purer target cells using the centrifuge bag only once.

For reference, a partition member for separating the upper and lower separation space may be formed on the body.

The centrifugal separation method, the centrifugal separator, and the centrifugal bag of the present invention physically separate the centrifuge space and the outlet by using a pressure member, thereby accommodating the target material in the process of obtaining the target cells from the target material through centrifugation. It is possible to prevent the surroundings of the outlet of one centrifugal bag from being damaged by high pressure or to leak the target material through the outlet.

The centrifugal separation method, the centrifugal separator, and the centrifugal bag of the present invention are fixed to the centrifugal bag by the adapter body to prevent the centrifugal bag from crushing during the centrifugal separation process, and thus the material inside the centrifugal bag It can prevent mixing.

The centrifugal separation method, the centrifugal separator, and the centrifugal bag of the present invention provide a pressure member directly to the adapter body without installing the centrifugal bag, and immediately after centrifugation without separating the centrifugal bag from the adapter. Allow collection of the desired material. Thus, in the process of separating the centrifuge bag from the adapter after centrifugation, it is possible to prevent the material inside the centrifuge bag from being mixed.

The centrifugal bag of the present invention uses a relatively inexpensive synthetic resin, and furthermore, by fusion welding the synthetic resin film simply, the manufacturing cost of the centrifugal bag is reduced.

1 is an exploded perspective view of a centrifugal separator and a centrifugal bag according to the present invention.

FIG. 2 is a combined perspective view of FIG. 1.

3 is a perspective view of the centrifugal bag shown in FIG.

4 is a perspective view of an adapter body to be inserted into a rotating bucket and a centrifugal bag mounted therein;

FIG. 5 is a perspective view of an adapter body equipped with a centrifugal bag and a rotating bucket to which the adapter body is mounted as shown in FIG. 4.

6 to 11 are front views for explaining the centrifugal separation method of the present invention.

6 is a front view of the adapter body with the bag receiving space exposed.

7 is a front view with the centrifuge bag mounted on the lower adapter body member.

8 is a front view of the first pressing member coupled to the lower adapter body member.

9 is a front view combining the upper adapter body member and the lower adapter body member.

10 is a front view of the second pressing member coupled to the adapter body after centrifugation.

11 is a front view of the first pressing member separated to discharge the target substance.

12 is a perspective view for explaining the centrifugal separator according to another embodiment of the present invention.

Figure 13 is a perspective view for explaining the centrifugal apparatus according to another embodiment of the present invention.

14 is an exploded perspective view of a centrifugal separator according to another embodiment of the present invention.

15 is a perspective view of fixing the centrifugal bag using the centrifugal separator shown in FIG.

16 is an exploded perspective view of a centrifugal separator according to another embodiment of the present invention.

FIG. 17 is a cross-sectional view of the centrifugal bag fixed using the centrifugal separator shown in FIG. 16 and inserted into the rotating bucket.

18 is a view showing a separator for centrifugation according to another embodiment of the present invention.

19 is a plan view of a separator for centrifugation according to another embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings, but the present invention is not limited or limited by the embodiments. For reference, in the present description, the same numbers refer to substantially the same elements, and may be described by referring to the contents described in the other drawings under these rules, and the contents determined to be obvious to those skilled in the art or repeated may be omitted.

Figure 1 is an exploded perspective view of the centrifugal apparatus and centrifugal bag according to the present invention, Figure 2 is a perspective view of the combination of Figure 1, Figure 3 is a perspective view of the centrifuge bag shown in Figure 1, Figure 4 is inside the rotating bucket A perspective view of an adapter body to be inserted into and a centrifugal bag mounted therein, FIG. 5 is a perspective view of an adapter body equipped with a centrifugal bag and a rotating bucket to which the adapter body is mounted as shown in FIG. 4.

1 to 5, the centrifuge bag 100 for separating the target material from the target material using centrifugation is a bag body 110, a fusion unit 120, and a pressure member mounting hole 130. ).

The bag body 110 is formed by fusion of a synthetic resin film formed using a synthetic resin of a flexible material, the edge of the bag body 110 is fused to form a centrifugal separation space 101 to be sealed with an external space therein. However, the entire edge is not fused, but the centrifugation space after the centrifugation in the inlet 104 and the lower portion of the bag body 110 for injecting the target material into the centrifuge space 101 on the upper portion of the bag body 110 It is fused except for the outlet 106 for discharging the target substance in 101. For reference, in the present embodiment, two injection holes 104 are provided, one on the left side and the other on the right side of the bag body 110, but in some cases, the number of injection holes may be changed.

In addition, each of the inlet 104 and the outlet 106 may be provided with a valve member 140 to selectively open and close them, each of the valve member may be used tongs, valves, zippers, etc., as the embodiment It may also be provided in the form of a stopper inserted and inserted into the 104 and the outlet 106. The valve member may be provided to be easily inserted into and removed from the inlet 104 and the outlet 106 using cork or synthetic resin, and may not be easily separated from the inlet 104 and the outlet 106, and the cannula Sharp and thin tubes, such as cannulas and needles, may be used to inject or discharge the desired material. In addition, as in the present embodiment, the valve member 140 in the form of a stopper is mounted to the outlet 106 so as to move toward the inside of the centrifuge space 101 from the outlet 106 by an external force, and the valve member 140. When is pushed inward, the target material can be discharged through a space formed between the outer surface of the valve member 140 of the stopper shape and the inner surface of the outlet 106.

In addition, the bag body 110 is formed by using a synthetic resin so that the shape can be flexibly changed when the target material is introduced into the centrifuge space 101 therein, for example, polyvinyl chloride or , Polymers of dimethylsiloxane and polycarbonate can be used. In particular, the polyvinyl chloride (polyvinyl chloride) may be suitable as a material of the bag body 110 because it is absorptive but elastic and resistant to chemicals. In addition, the centrifugal bag 100 according to the present invention is used for the purpose of storing the target material extracted from the target material for a certain period of time, as well as centrifugation, in particular, in the case of culturing the target material in addition to the above-described characteristics In this case, a material that can permeate is preferable, and in this case, the material of the bag body 110 is made of dimethylsiloxane and a polymer of polycarbonate, so that permeation of liquid is prevented and permeable to a specific gas such as oxygen. It can also manufacture.

In the manufacturing process of the centrifugal bag 100, the edge portion, that is, the synthetic resin film around the bag body is fused along the shape of the adapter body 210, the fusion portion 120 is fixed by the adapter body 210 Form.

In addition, a portion of the fusion portion 120 is cut or provided in a state in which holes are formed in the synthetic resin film itself before fusion, and thus, a pressing member mounting hole (not shown) enables mounting of the pressing member to both sides of the lower separation space 103 ( 130, which will be described later in detail.

In addition, the target material may be centrifuged so that the target material collected in the lower separation space 103 of the bag body 110 is concentrated around the outlet 106. For example, in order to facilitate the acquisition of the target substance through the outlet 106, the bag body 110 may have a width in the direction of the outlet 106 from the upper separation space 102 located above the lower separation space 103. It may be formed to narrow gradually, specifically, the lower side and the lower side of the lower separation space 103 of the upper separation space 102 connected to the lower separation space 103 may be formed to have a different inclination angle. In general, the lower separation space 103 may be fused in an inclined U-shape, or may be fused in a more urgently inclined V-shape. If the receiving space inside the body 110 becomes too small, on the contrary, if the bottom of the upper separating space 102 is formed to be horizontal or too slanted, material movement from the upper separating space 102 to the lower separating space 103 is smooth. You may not. Therefore, the bottom of the upper separation space 102 is preferably manufactured to have an inclined angle θ of 15 degrees to 85 degrees.

For reference, the valve member 140 may be inserted into the non-fused inlet 104 or the outlet 106 after the two synthetic resin films are fused, and may be inserted between the films before the two synthetic resin films are fused. It may be arranged first, and the film may be fused to be disposed at the inlet 104 or the outlet 106.

Centrifugal bag 100 of the present embodiment can be manufactured through a simple manufacturing process such as fusion using a flexible material such as plastic, it is possible to reduce the manufacturing cost, thereby burdening the centrifuge bag 100 It is possible to use disposable without. In addition, since no reuse is necessary, a purer target material can be extracted.

Hereinafter, the centrifugal separator which is equipped with the above-mentioned centrifugal bag and which can centrifugate the target substance contained in the centrifugal bag is demonstrated in detail.

Referring back to Figures 1 to 5, the centrifugal bag 100 described above is accommodated in the adapter body 210, it can be fixed therein by the adapter body 210, the adapter body 210 is The rotating bucket 205 is again mounted on the rotating bucket 205, the rotating bucket 205 is mounted on the rotary rotor of the centrifugal separator may be rotated at a specific speed to centrifuge the object contained in the centrifuge bag 100.

The centrifugal separator for centrifuging the object by rotating the rotary bucket 205 includes an adapter body 210, a rotary bucket 205, and a pressure member 260, and in addition, rotates the rotary bucket 205. It may further include a control unit for adjusting the rotational speed of the motor unit and the motor, etc., which is a configuration of a centrifugal separator for driving in a general swing-out method can be omitted herein.

The adapter body 210 may provide a bag accommodation space 212 therein to accommodate the centrifuge bag 100, and may fix the centrifuge bag 100 therein. Specifically, in the present embodiment, the adapter body 210 includes an upper adapter body member 220 and a lower adapter body member 240 that overlap each other to form a bag receiving space 212 therein. In addition, the shape of the adapter body 210 is determined so as to be tightly fixed inside the rotating bucket 205, the bottom of the adapter body 210 to expose the outlet 106 of the centrifuge bag 100 It is formed to be recessed around the outlet 106. For reference, an upper magnet 229 and a lower magnet 249 may be disposed at corner portions of the upper adapter body member 220 and the lower adapter body member 240 to be coupled magnetically.

The upper adapter body member 220 is coupled to the lower adapter body member 240 to selectively expose the bag receiving space 212. In the present embodiment, the upper adapter body member 220 is connected to the lower adapter body member ( 240 is hinged to the side, and may rotate from the upper adapter body member 220 to selectively expose the bag receiving space (212).

In addition, the upper adapter body member 220 and the lower adapter body member 240 are coupled to each other, by pressing the fusion unit 120 of the centrifuge bag 100 disposed in the bag receiving space 212 from both sides to centrifuge. Separation bag 100 may be prevented from flowing inside the bag receiving space 212, or the shape of the centrifuge bag 100 is distorted during centrifugation.

Specifically, the upper fixing part 221 and the lower fixing part 241 are provided inside the upper adapter main body member 220 and the lower adapter main body member 240, respectively. The upper fixing part 221 and the lower fixing part 241 may press the edges of the centrifugal bag 100 at both sides when the upper adapter body member 220 and the lower adapter body member 240 are combined. To this end, the centrifuge bag 100 may be fixed inside the adapter body 210.

In this embodiment, the lower fixing part 241 is a lower surface fixing part 242 protruding from the bottom of the lower adapter body member 240 to support a part of the edge of the fusion portion 120, and the lower adapter body member The lower point fixing part 243 protrudes from the bottom so as to locally support any one point of the fusion part 120.

On the other hand, the upper fixing portion 221 and the upper surface fixing portion 222 protruding from the bottom surface of the upper adapter body member 220 to be in close contact with the lower surface fixing portion 242 and the lower point fixing portion 243 and And an upper point fixing part 223 protruding from the bottom of the upper adapter body member 220 to be in close contact.

In the present embodiment, the lower surface fixing part 242 is formed to correspond to a partial region of the fusion part 120 formed around the inlet 104 and the outlet 106 of the centrifugal bag 100, and the upper part corresponding thereto. The surface fixing part 222 is formed. In addition, the lower point fixing unit 243 is formed to correspond to a point of the fusion unit 120 on both sides with the outlet 106 therebetween, and the upper point fixing unit 223 is formed correspondingly.

When the upper adapter body member 220 and the lower adapter body member 240 are stacked and combined, the centrifugal bag 100 is closely adhered by the upper fixing portion 221 and the lower fixing portion 241 on both sides of the adapter body. It may be fixed not to flow in the bag receiving space (212) provided inside (210).

Of course, the shape of the upper fixing portion 221 and the lower fixing portion 241 may be changed, specifically, the upper fixing portion 221 and the lower fixing portion 241 is the fusion portion of the centrifugal bag 100 ( The size or position of the area supporting the 120 may be changed, and the number thereof may also be changed.

In addition, a bottom surface of the lower adapter body member 240 is provided with a body binding portion 244 for binding the pressing member 260 to be described later. In the present embodiment, the main body binding unit 244 includes an upper main body binding unit 245 provided in pairs at the top and a lower main body binding unit 246 provided in pairs under the upper main body binding unit 245.

The pressing member 260 may physically separate the centrifugal space 101 provided in the centrifugal bag 100, and in this embodiment, the pressing member 260 may include the first pressing member 261 and the second pressing member. And a pressing member 265.

The first pressing member 261 may press the front and rear portions of the centrifugal bag 100 from the outside to physically separate the centrifugal space 101 and the outlet 106, and the second pressing member 265 may The front and rear portions of the centrifugal bag 100 may be pressurized from the outside to divide the centrifugal space 101.

The first pressing member 261 binds to the lower body binding portion 246 to bind the first pressing bar 262 provided in the form of a bar, and the first pressing bar 262 to the lower adapter body member 240. And a first pressing member binding unit 263 to be used. In this case, the first pressing member binding unit 263 is provided in the form of a screw that is attached to the female thread formed in the lower body binding unit 246 through holes formed at both sides of the first pressing bar 262.

The second pressing member 265 is also coupled to the second pressing bar 266 provided in the form of a bar, and the upper body binding portion 245 to bind the second pressing bar 266 to the lower adapter body member 240. The second pressing member engagement portion 267 is included. In this case, the second pressing member binding unit 267 may be provided in the form of a screw that is attached to the female screw unit formed in the upper body binding unit 245 through holes formed at both sides of the second pressing bar 266.

Of course, the first pressing member 261 and the second pressing member 265, as described above, in addition to the screw coupling method in the form of a clip or hook coupling method, etc., the lower body binding portion 246 and the upper body binding portion 245 It may be provided to be combined with.

For reference, the pressing member mounting holes 130 formed in the fusion unit 120 in the centrifugal bag 100 may be mounted to each other so that the first pressing member 261 or the second pressing member 265 may be mounted. The first pressing member mounting hole 132 and the second pressing member mounting hole 134 are closed.

Meanwhile, the upper adapter body member 220 includes an exposure hole 224 exposing the bag receiving space 212. Accordingly, even when the upper adapter body member 220 and the lower adapter body member 240 are bound, that is, in the closed state, the upper body binding part 245 and the lower body binding part 246 are exposed to the outside through the exposure hole 224. Can be. Accordingly, the first and second pressure bars 266 may be directly coupled to the upper body binding portion 245 and the lower body binding portion 246 through the exposure hole 224.

For reference, the lower adapter body member 240 has a centrifugal bag 100 that protrudes from an inner surface of the lower adapter body 210 and is pressurized by the first pressure bar 262 and the second pressure bar 266. The first support bar 251 and the second support bar 252 are supported at the bottom.

Hereinafter, the centrifugal separation method using the centrifugal bag and centrifugal separator described above will be described in detail.

First, as shown in FIG. 6, the upper adapter body member 220 is rotated from the lower adapter body member 240 to expose the bag accommodation space 212 provided on the lower adapter body member 240.

Thereafter, as shown in FIG. 7, the centrifuge bag 100 is placed in the corresponding bag receiving space 212 on the lower adapter body member 240.

Thereafter, as shown in FIG. 8, the centrifugal space 101 and the outlet 106 of the centrifugal bag 100 are physically separated using the first pressing member 261. Specifically, the first pressing bar 262 is disposed such that holes provided at both ends of the first pressing bar 262 correspond to the lower main body binding portion 246, and the first pressing member binding portion 263 is used. The first pressure bar 262 is fixed to the lower body binding portion 246.

Thereafter, as shown in FIG. 9, the upper adapter body member 220 is rotated to engage the lower adapter body member 240. At this time, the centrifugal bag 100 is fixed in the bag receiving space 212 by the upper fixing portion 221 and the lower fixing portion 241 as described above.

For reference, since the lower body binding unit 246 is exposed to the outside through the exposure hole 224 formed in the upper adapter body member 220, the lower adapter body member 240 is rotated by rotating the upper adapter body member 220. After binding with, the centrifugal space 101 and the outlet 106 of the centrifugal bag 100 may be physically separated using the first pressing member 261.

Thereafter, the adapter body 210 may be mounted on the rotating bucket 205 to undergo a centrifugation process. At this time, the centrifugal space 101 and the discharge port 106 are centrifuged in a state of being physically separated by the first pressing member 261.

Thereafter, as shown in FIG. 10, after removing the adapter body 210 from the rotating bucket 205, the centrifugal space 101 can be divided using the second pressing member 265. In this case, the second pressing member 265 may selectively separate a region between materials in addition to the target material.

Thereafter, as shown in FIG. 11, in order to release the state in which the centrifugal space 101 and the outlet 106 are physically separated, the first pressing member 261 is separated and the second pressing member 265 is removed. In the state of), only the target substance can be obtained through the outlet 106.

12 is a front view showing another type of centrifugal separator according to the present invention. Referring to FIG. 12, the centrifugal separator according to the present embodiment is substantially the same as the centrifugal separator described in the above embodiment. Therefore, the description of the centrifugal separator in the present embodiment can refer to the description and the drawings of the centrifugal separator of the previous embodiment, the repeated content can be omitted. However, the shape of the pressing member in the centrifugal separator of this embodiment is slightly different from the previous embodiment, and will be described in detail with reference to this. In addition, in the centrifugal bag, the same number can be given.

In this embodiment, the urging member 361 includes a urging bar 362 and a urging member engagement portion 363. In the previous embodiment, the centrifugal space 101 and the outlet 106 are physically separated from each other by using the first and

second pressure members

261 and 265 having pressure bars of different lengths, and the centrifugal space 101 ), But in this embodiment, the pressure member 361 physically separates the centrifugal space 101 and the outlet 106 by using a single pressure bar 362, and the centrifugal space 101 Also used to split).

Specifically, the pressure bar 362 is the lower body binding portion so that the pressure bar 362 physically separates the centrifuge space 101 and the outlet 106 of the centrifuge bag 100 before centrifugation. The pressure member binding unit 363 may be coupled to the lower main body binding unit 346. Thereafter, after centrifuging the target material in a state in which the centrifugal space 101 and the outlet 106 of the centrifugal bag 100 are physically separated, the pressure member binding part 361 is connected to the lower body binding part. Removed from 346, the pressure bar 362 is positioned on the upper body binding portion 345, the pressure member binding portion 363 is coupled to the upper body binding portion 345, the pressure bar 362 By the centrifugation space 101 to be physically divided. In this case, the centrifugal space 101 and the outlet 106 are physically separated from each other.

Figure 13 is a front view showing another type of centrifugal separator according to the present invention, referring to Figure 13, the centrifugal separator according to the present embodiment is substantially the same as the centrifugal separator described in the previous embodiment. Therefore, the description of the centrifugal separator in the present embodiment can refer to the description and the drawings of the centrifugal separator of the previous embodiment, the repeated content can be omitted. However, the shape of the pressing member in the centrifugal separator of this embodiment is slightly different from the previous embodiment, and will be described in detail with reference to this. In addition, in the centrifugal bag, the same number can be given, and the centrifugal bag of this embodiment is different from the centrifugal bag described above, and the pressing member mounting hole formed in the fusion part for sliding movement of the pressure bar is pressed. It can be formed integrally so as not to interfere with the sliding movement of the bar.

In the present embodiment, the pressing member 461 includes a pressing bar 462 and the pressing member engagement portion 463. The pressure member 461 is used to physically separate the centrifugal space 101 and the discharge port 106 by using a single pressure bar 462, and to divide the centrifugal space 101.

Specifically, the pressure bar 462 to the discharge port 106 so that the pressure bar 462 physically separates the centrifugation space 101 and the discharge port 106 of the centrifuge bag 100 before centrifugation. The pressure member engagement portion 463 may be coupled to a hole formed in the support bar 447 at a height adjacent to the support bar 447. Thereafter, after centrifuging the target material in a state in which the centrifugal space 101 and the outlet 106 of the centrifugal bag 100 are physically separated, the pressure member binding part 463 is supported by the support bar 447. And the pressure bar 462 is formed on the support bar 447 again after the pressure bar 462 is disposed at the height to divide the separation bag space. The pressure member binding part 463 is coupled to the hole so that the centrifugal space 101 is physically divided by the pressure bar 462. In this case, the centrifugal space 101 and the outlet 106 are physically separated from each other.

On the other hand, the support bar 447 which can move while corresponding to the position of the pressure bar 462 supports both ends of the pressure bar 462 inside the lower adapter body member 440, and grooves 449 into which both ends are inserted. The guide rail 446 is formed.

Accordingly, the support bar 447 may slide up and down along the lower adapter body member 440 in accordance with the movement position of the pressure bar 462.

Figure 14 is an exploded perspective view of the centrifugal separator according to another embodiment of the present invention, Figure 15 is a perspective view of fixing the centrifugal bag using the centrifugal separator shown in FIG.

14 and 15, the centrifugal adapter 500 for fixing the centrifugal bag 20 made of a flexible material in the rotating bucket 10 of the centrifugal separator may be formed to be symmetrical with each other. Two

vertical incisions

510, 520.

Each of the first and second

vertical incision bodies

510 and 520 is provided as a frame having a frame shape formed along the circumference of the centrifugal bag 20.

Specifically, the first vertical incision body 510 is a rotary bucket 10 integrally with the first horizontal movement preventing portion 511 and the first horizontal movement preventing portion 511 whose both ends contact the inner surface of the rotating bucket 10. It includes a first vertical support 512 extending in the longitudinal direction of the.

In this case, the first horizontal movement preventing part 511 corresponds to a horizontal frame in which both ends contact the inner surface of the rotating bucket 10 at predetermined intervals in the longitudinal direction of the rotating bucket 10, and includes a first vertical support part ( 512 corresponds to a longitudinal frame extending in the longitudinal direction of the rotating bucket 10 to connect between the horizontal frame.

Similarly, the second vertical incision body 520 integrally with the second horizontal movement preventing part 521 and the second horizontal movement preventing part 521 which contacts the inner surface of the rotating bucket 10 in the longitudinal direction of the rotating bucket 10. It includes a second vertical support 522 extending to.

In this case, the second horizontal movement preventing part 521 corresponds to a horizontal frame in which both ends contact the inner surface of the rotating bucket 10 at predetermined intervals in the longitudinal direction of the rotating bucket 10, and the second vertical support part ( 522 corresponds to a longitudinal frame extending in the longitudinal direction of the rotating bucket 10 to connect between the horizontal frame.

As described above, the first and second vertical incision bodies (510, 520) are coupled to each other with the centrifuge bag (20) in between, each of the first and second horizontal movement preventing portion (511, 521) Both ends may contact the inner surface of the rotary bucket 10 to prevent the combined first and second

vertical incision bodies

510 and 520 from moving horizontally inside the rotary bucket 10.

In addition, the first and second

vertical supports

512 and 522 are in close contact with both sides of the centrifugal bag 20 so that the centrifugal force of the centrifugal bag 20 in the rotating bucket 10 during the centrifugal separation process is increased. Compression deformation in the acting direction can be prevented.

Specifically, a pair of first and second

vertical support portions

512 and 522 facing each other of the first vertical incision body 510 and the second vertical incision body 520 may be a pair of centrifugal bags 20. One side is fixed and the other pair is fixing the other side of the centrifuge bag 20.

On the other hand, the rotary bucket 10 of the present embodiment is formed with insertion grooves 12 into which both side portions of the combined first and second

vertical incision bodies

510 and 520 are inserted, and the combined first and second vertical Both sides of the cutting

bodies

510 and 520 may be inserted into the rotary bucket 10 through the insertion groove 12, and the first and second vertical cutting

bodies

510 and 520 coupled thereto may be inserted into the rotary bucket 10. 10) movement in the horizontal direction is prevented at the same time and rotation along the inner surface of the rotary bucket 10 is prevented. At this time, by forming the width of the insertion groove 12 is substantially the same as the width of the combined first and second vertical incision body (510, 520) and the entire centrifuge bag 20 interposed therebetween, The first and second vertical incision bodies (510, 520) may be more tightly adhered to the centrifuge bag (20).

As shown in FIG. 15, the insertion groove 12 may be formed by digging a groove extending from the open inlet of the rotary bucket 10 to the bottom surface, and in some cases, the groove may be extended to the predetermined depth from the inlet. Parsers may be formed, and both side portions of the first and second

vertical cutout bodies

510 and 520 coupled to each other may be formed to have different widths so as to have a step corresponding to the groove.

Meanwhile, the first and second

vertical incision bodies

510 and 520 coupled to each other so that the first and second

vertical incision bodies

510 and 520 coupled with the centrifugal bag 20 therebetween are more firmly coupled to each other. A coupling member for tightly fixing the liver is provided.

The coupling member includes a bolt 531 penetrating the first and second

vertical incision bodies

510 and 520 and a nut 532 tightened from the opposite side with the first and second

vertical incision bodies

510 and 520 interposed therebetween. And a hole through which the bolt 531 penetrates the first and second

vertical cut bodies

510 and 520. The bolt 531 may penetrate only the first and second

vertical incision bodies

510 and 520, but may also penetrate the centrifugal bag 20 interposed therebetween.

Thus, the centrifugal bag 20 in close contact between the first and second

vertical incision bodies

510 and 520 may be more effectively prevented from being deformed in the centrifugal force direction during centrifugation.

For reference, the first and second vertical incision body (510, 520) may be integrally connected so that any one of the first and second vertical incision body (510, 520) is rotatable from the other, specifically the same material It is also possible to connect the two incision body by injection using a separate, it can be coupled to each other using a hinge, such as a separate folding.

In addition, the first and second vertical incision body (510, 520) four injection hoses connected to the centrifuge bag (20) at the top of the centrifuge bag (20) and the lower part of the centrifuge bag (20) Receiving grooves corresponding to the one discharge hose is connected to the centrifugal bag 20 in the formed in the first, when the first and second vertical incision body (510, 520) is coupled, the first and A gap may occur between the second

vertical cut bodies

510 and 520, or the hoses may be prevented from being compressed.

When the first and second

vertical incision bodies

510 and 520 are formed in a frame as in the present embodiment, the first and second

vertical incision bodies

510 and 520 are combined to be inserted into the rotating bucket 10. At this time, an empty space is formed relatively wide between the rotary bucket 10 and the centrifugal adapter 500.

This can be inserted into the centrifugal adapter 500 as it is, and fixed in the rotary bucket 10 without removing the incident injection hose and the discharge hose connected to the centrifugal bag 20.

By not separating the centrifugal bag 20 and the additional hoses connected thereto, the plurality of centrifugal bags 20 are connected to the inside of the bag at the same time with the injection hose and the discharge hose described above. Filling and inserting them into different rotary buckets 10, respectively, it can be carried out a centrifugation process at a time.

This has the advantage of obtaining a desired material efficiently by injecting a large amount of material into a plurality of centrifugal bags 20 at a time, and also because it does not separate into each centrifugal bag 20, it is for centrifugal separation. The material filled in the bag 20 may be minimized to expose the bacteria or foreign substances to the outside.

For reference, the first and second

vertical incision bodies

510 and 520 provided as a frame in the form of a frame of the present embodiment are generally within a range in which their shape is not deformed in the rotating bucket 10 which rotates at a speed of more than several thousand rpm. It can be produced using a variety of materials, such as metal and synthetic resin, the present invention is not limited by the material.

Hereinafter, the centrifugal adapter according to another embodiment is substantially the same as the centrifugal separator described in the previous embodiment. Therefore, in the present embodiment, the description of the I centrifugal separator may refer to the description and the drawings of the centrifugal separator of the previous embodiment, and the repeated content may be omitted.

However, this embodiment will be described in more detail with respect to the anti-vibration portion and the coupling member that is different from the components of the previous embodiment.

FIG. 16 is an exploded perspective view of a centrifugal separator according to another embodiment of the present invention, and FIG. 17 is a cross-sectional view of the centrifugal separator shown in FIG.

16 and 17, the centrifugal adapter 600 for fixing the centrifugal bag 20 made of a flexible material in the rotary bucket 10 of the centrifugal separator is formed to be symmetrical with each other. Two

vertical incisions

610 and 620.

In this embodiment, the first and second vertical incision body (610, 620) is integrally connected so that any one of the first and second vertical incision body (610, 620) is rotatable from the other one, One side of each of the first and second

vertical incision bodies

610 and 620 is connected to form an integrated body. Of course, it may be combined integrally using a hinge, such as folding.

Each of the first and second

vertical incision bodies

610 and 620 is provided as a frame having a frame shape formed along the circumference of the centrifugal bag 20.

Specifically, the first vertical incision body 610 is a rotary bucket 10 integrally with the first horizontal movement preventing portion 611 and the first horizontal movement preventing portion 611 whose both ends contact the inner surface of the rotating bucket (10). A first vertical support 612 extending in the longitudinal direction thereof.

Similarly, the second vertical incision body 620 is integrally formed with the second horizontal movement preventing part 621 and the second horizontal movement preventing part 621 which contact the inner surface of the rotating bucket 10 in the longitudinal direction of the rotating bucket 10. It includes a second vertical support 622 extending to.

As described above, the first and second vertical incision body (610, 620) are coupled to each other with the centrifuge bag (20) in between, each of the first and second horizontal movement preventing portion (611, 621) Both ends may contact the inner surface of the rotary bucket 10 to prevent the combined first and second

vertical incision bodies

610 and 620 from moving horizontally inside the rotary bucket 10.

In addition, the first and second

vertical support portions

612 and 622 are in close contact with both sides of the centrifugal bag 20 so that the centrifugal force of the centrifugal bag 20 in the rotating bucket 10 during the centrifugal separation process is increased. Compression deformation in the acting direction can be prevented.

On the other hand, in the present embodiment, like the above-described embodiment, the insertion groove is not formed in the rotary bucket 10, and the first and second anti-tilts on the first and second

vertical incision bodies

610 and 620, respectively. 613 and 623 are formed.

The length of the rotary bucket 10 from the first and second horizontal

movement prevention parts

611 and 621 is located at the bottom and close to the bottom surface of the rotary bucket 10, respectively. It extends perpendicular to the direction. Accordingly, the first and second

vertical incision bodies

610 and 620 are combined to prevent the first and second

vertical incision bodies

613 and 623 from being inclined inside the rotary bucket 10.

Meanwhile, the first and second

vertical incision bodies

610 and 620 coupled to each other so that the first and second

vertical incision bodies

610 and 620 coupled with the centrifugal bag 20 therebetween are more firmly coupled to each other. A coupling member for tightly fixing the liver is provided.

The coupling member is provided on the other side opposite to the connected side of the first and second

vertical incision bodies

610 and 620, and the coupling member is a clip for closely contacting the first and second

vertical incision bodies

610 and 620 on both sides. 633 is provided. Accordingly, the first and second

vertical incision bodies

610 and 620 closely adhere to the sides of the centrifugal bag 20, thereby preventing the compressive deformation in the centrifugal force direction more effectively.

For reference, grooves accommodating the coupling member are formed in the first and second

vertical incision bodies

610 and 620 to which the coupling member is coupled. Accordingly, the coupling member prevents the first and second horizontal

movement preventing parts

611 and 621 from being spaced apart from the inner surface of the rotating bucket 10 by touching the inner surface of the rotating bucket 10.

In addition, the first and second vertical incision body (610, 620) in the upper portion of the centrifuge bag (20) and the four injection hoses connected to the centrifuge bag (20) and the bottom of the centrifuge bag (20) Receiving grooves corresponding to the one discharge hose is connected to the centrifuge bag 20 is formed in the first, and when the first and second vertical incision body (610, 620) is coupled, the first and second by the hose A gap may occur between the second

vertical cut bodies

610 and 620, or the hoses may be prevented from being compressed.

Referring to FIG. 18, a centrifugal separator 700 for separating target cells from tissues extracted from living organisms using centrifugation may include an upper separation bag 710, a lower separation bag 720, and a partition member ( 730).

The upper separation bag 710 forms an upper separation space 711 using a flexible material, and separates the treatment solution from the tissue injection port 712 and the tissue injection port 712 for injecting tissue into the upper separation space 711. A first processing solution inlet 713 for injecting, a second processing solution inlet 714, and a solution outlet 715 for discharging the residual material in the upper separation space 701 after centrifugation. For reference, two

treatment solution inlets

713 and 714 for injecting treatment solution are provided in this embodiment, but may be further formed by the amount of treatment solution desired by the user.

In some cases, a plurality of treatment solutions may be injected through one treatment solution inlet, but the pre-injected treatment solution remains in the treatment solution inlet and then injected into the upper separation space 701 together with the treatment solution to be injected. It is preferable that the number is determined according to the number of types of processing solution to be used so as to prevent it.

The lower separating bag 720 is also formed integrally with the upper separating bag 710 by using a flexible material, and forms a lower separating space 721 connected to the upper separating space 711, from the lower separating space 721. And a target outlet 722 for discharging the target cell.

The partition member 730 is positioned between the upper separation space 711 and the lower separation space 721 to selectively open and close a passage connecting the upper separation space 711 and the lower separation space 721.

The upper separation bag 710 is formed in a funnel shape in which the width of the upper separation bag 710 gradually decreases toward the lower separation bag 720, so that the target cells are easily separated in the lower separation bag 720 during centrifugation. To be introduced toward the), the width of the lower separation bag 720 is formed to be smaller than the width of the upper separation bag 710, but to have the same width in the longitudinal direction.

In addition, the upper separation bag 710 is a valve member 701 for selectively opening and closing the tissue inlet 712, the first treatment solution inlet 713, the second treatment solution inlet 714, and the solution outlet 715 In addition, the lower separation bag 720 also includes a valve member 701 for selectively opening and closing the target outlet 722.

In the present embodiment, the valve member 701 may be provided separately with tongs, valves, and zippers. However, since the upper and

lower separating bags

710 and 720 of the present embodiment are formed of a plastic material having flexibility, virtually, The inlet 712, the first treatment solution inlet 713, the second treatment solution inlet 714, the solution outlet 715, and the target outlet 722 are all provided in a blocked state, and a cannula, and a needle. Sharp and thin tubes can be used to inject or discharge the desired material. That is, when the valve member 701 is made of a synthetic film, the valve member 70 may be integrally formed with the upper and

lower separation bags

710 and 720, and the centrifugal separation system may be immediately implemented by simply connecting the pipes. It is easy to manufacture.

In addition, the upper and lower separating bags (710, 720) has a sealing property with the outside, using a plastic material having an elasticity, such as polyvinyl chloride (polyvinyl chloride) is not absorbent but elastic and has a high resistance to chemicals Can be used.

However, when the centrifugal separator 700 according to the present invention is used for the purpose of culturing the target cells extracted from the tissue as well as centrifugal separation, it is also possible to use a material that can penetrate a specific gas in addition to the above characteristics. For example, a liquid may not permeate, but an oxygen enrichment membrane prepared using a polymer of dimethylsiloxane and polycarbonate to permeate only oxygen may be used.

Thus, the centrifugal separator 700 of the present invention is the injection of the necessary tissue in the process of obtaining the target cells from the tissue, the injection of the treatment solution, the discharge of residual substances generated after the reaction of the treatment solution and the tissue, and the desired purpose The discharge of the cells through the different passages can be prevented from mixing with each other in detail, by using the valve member 70, or by using a sharp tool such as cannula, external dust or viruses, It is thoroughly blocked from contaminants such as bacteria.

In addition, the lower separating bag 720 may be separated from the upper separating bag 710 in a sealed state using the partition member 730, and the partition member 730 may use a forceps or a valve.

Therefore, after centrifuging the tissue using the apparatus 700, the target cells may be stored separately in the state of being stored in the lower separation bag 720.

The centrifugal separator according to the present embodiment is substantially the same as the centrifugal separator described in the previous embodiment. Therefore, the description of the centrifugal separator in the present embodiment can refer to the description and drawings of the centrifugal separator of the previous embodiment, the repeated content can be omitted. In the present embodiment, for the convenience of description, the same components will be described using the same reference numerals.

However, this embodiment will be described in more detail with respect to the manufacturing method of the centrifugal separator.

In order to manufacture a centrifugal separator 800 for separating target cells from tissues extracted from living organisms using centrifugation, first, the resin films using two sheets of synthetic resin are disposed to face each other. Two resin films formed using a flexible synthetic resin may be fused through pressure, heating, high frequency, and chemical reaction to form a film separation bag 20.

In addition, the above-mentioned resin film has an airtightness with the outside, using a plastic material having elasticity, there is no water absorption, such as polyvinyl chloride (polyvinyl chloride) can be used a material having a high elasticity and resistance to chemicals.

However, when the centrifugal separator 800 according to the present invention is used for the purpose of culturing the target cells extracted from the tissue as well as centrifugal separation, it is also possible to use a material that can penetrate a specific gas in addition to the above characteristics. .

Referring to FIG. 2, the inside of the film separation bag 20 provides an upper separation space 811 and a lower separation space 821, and a tissue injection hole 812 for injecting tissue into the upper separation space 811. ), First and second treatment solution inlets 813 and 214 for injecting treatment solution separately from the tissue inlet 812, and a solution outlet 815 for discharging residual material in the upper separation space 811 after centrifugation. ) Is provided, and the lower portion of the lower separation space 821 is formed with a target discharge port 822 for discharging the target cells collected in the lower separation space 821, the upper separation space 811 and the lower separation space 821 The partition member 830 selectively opens and closes a passage connecting the upper separation space 811 and the lower separation space 821.

The arrangement of the partition member 830 is fused around the space into which the partition member 830 is to be inserted when the two films 10 described above are fused, and the portions are separated, thereby separating the upper separation space 811 and the lower separation. The space 821 may be sealed to the outside, and the partition member 830 may be inserted.

Further, when the film separation bag 20 is fused through the method described above, the tissue inlet 812, the solution outlet 815, the first and second treatment solution inlets 813 and 214, and the target outlet port ( The edge of the film separation bag 20 except for 822 is sealed by the fusion portion A formed by fusion, and in particular, the width of the upper separation space 811 toward the lower separation space 821 is fused to gradually decrease. It may be sealed, while the width of the lower separation space 821 may be fused to remain the same, and in some cases, the material collected in the lower separation space 821 may be concentrated around the target outlet 822. Thus, to facilitate the acquisition of the material through the target outlet 822, the lower separation space 821 is also fused in a U-shaped inclined gradually smoothly in the direction opposite the upper separation space 811, Fusion in the form of a V-shaped slanted more sharply Can.

Thereafter, as shown in FIG. 2, a valve for selectively opening and closing the tissue inlet 812, the first and second treatment solution inlets 813 and 214, the solution outlet 815, and the target outlet 822. Valve members 801 such as tongs may be disposed.

However, since the film separation bag 20 according to the present invention is formed of a plastic material having a predetermined elasticity, in fact, the tissue inlet 812, the first treatment solution inlet 813, the second treatment solution inlet 814, Both the solution outlet 815 and the target outlet 822 may be fused and provided in a clogged state, and a pointed thin tube such as a cannula and a needle may be used to inject or discharge a desired substance.

In addition, the incision line (L) is formed in the film separation bag 20 along the periphery of the lower separation space 821, and after centrifuging the tissue using the centrifugal separator 800 of the present embodiment, The lower separation space 821 may be cut out from the film separation bag 20 in a sealed state. In particular, the boundary between the upper separation space 811 and the lower separation space 821 may be separated from the film separation bag 20 in a blocked state using the partition member 830.

The centrifugal separator 800 according to the present embodiment provides a centrifugal separator 800 which can be manufactured through a simple manufacturing process such as fusion using a flexible material such as plastic, thereby reducing manufacturing costs. To this end, it is possible to use the centrifugal separator 800 for single use without burden. In addition, since it is not necessary to reuse, a more pure target cell can be extracted.

As described above, although described with reference to the preferred embodiment of the present invention, those skilled in the art various modifications and variations of the present invention without departing from the spirit and scope of the invention described in the claims below I can understand that you can.

The method, apparatus, and bag for centrifugation according to the present invention can be used for centrifugation to separate the target material from the subject material of the centrifugation.

Claims

A centrifugal separation bag having a centrifugal space for receiving a target material and an outlet for discharging the target material to the outside at the bottom of the centrifugal space, the centrifugal separation for separating the target material from the target material in the separation bag In the method,

Centrifuging the target material;

Dividing the centrifuge space; And

Maintaining the divided state and discharging a target substance from the divided space adjacent to the outlet side;

Centrifugation method comprising a.
The method of claim 1,

In the step of dividing the centrifuge space,

Centrifugal separation method characterized in that to separate the centrifugation space by pressing a portion before and after the separation bag from the outside.
The method of claim 2,

Before the centrifugation step, the centrifugation space and the outlet are physically separated from the separation bag,

In the step of discharging the target material, the centrifugation method characterized in that to release the state in which the centrifugation space and the discharge port physically separated.
The method of claim 3,

It provides an adapter body corresponding to the rotary bucket for centrifugation,

Physically separating the centrifugal space from the outlet by using a first pressing member mounted to the adapter body,

Centrifugal separation method characterized in that for dividing the centrifuge space by using a second pressing member mounted to the adapter body.
The method of claim 3,

An adapter body corresponding to a rotating bucket for centrifugation is provided, and the centrifugal space and the outlet are physically separated by using a first pressing member mounted on the adapter body, and the first pressing member is removed after centrifugation. Centrifugation method characterized in that for moving to divide the centrifuge space.
The method of claim 5,

The first pressing member is a centrifugal separation method characterized in that the slide can be moved and fixed up and down along the adapter body.
The method of claim 1,

A partition member for separating the centrifuge space up and down is formed in the separation bag,

And in the step of dividing the centrifuge space after centrifuging the target material, dividing the centrifuge space into the partition members.
Separating the target material from the target material in the separation bag using a separation bag including a centrifugation space for receiving the target material of the centrifugation and an outlet for discharging the target material to the outside at the bottom of the centrifuge space In the centrifugal apparatus for,

An adapter body providing a bag accommodation space therein to accommodate the separation bag and fixing the separation bag; And

A rotating bucket to which the adapter body is mounted;

And a centrifugal separator for centrifuging the target material by rotating the rotary bucket.
The method of claim 8,

Centrifugal apparatus further comprises a pressing member for physically separating the centrifugal space.
The method of claim 9,

And the pressure member selectively separates a region between the centrifuge space and the outlet or a region between the target substance and a substance other than the target substance after centrifugation.
The method of claim 9,

The pressing member,

A first pressurizing member which pressurizes a part of the front and rear parts of the separating bag from the outside to physically separate the centrifugal space and the discharge port, and prevents leakage from the discharge port while the rotary bucket rotates;

And a second pressurizing member which presses a part before and after the separating bag from the outside to divide the centrifuge space and prevents mixing with other substances during discharge of the target substance after centrifugation.
The method of claim 9,

The pressing member is fixed to the adapter body in a different position up and down,

The rotary bucket is fixed in a position to prevent leakage from the outlet during rotation,

A centrifugal separator characterized in that the separation bag is physically separated by being fixed at a position which prevents mixing with other substances while discharging the target substance after centrifugation.
The method of claim 12,

The pressing member is a centrifugal separator characterized in that the release, slide movement, and fixing with respect to the adapter body.
The method of claim 9,

The adapter body includes an upper adapter body member and a lower adapter body member overlapping each other to form the bag receiving space therein,

And the upper adapter body member is coupled to the lower adapter body member to selectively expose the bag receiving space.
The method of claim 14,

The lower adapter body member is provided with a body binding portion,

The pressurizing member includes a pressurizing bar provided in the form of a bar, and a pressurizing member binding part that is attached to the main body binding part to bind the pressure bar to the lower adapter body member.
The method of claim 15,

The upper adapter body member includes an exposure hole for exposing the bag receiving space,

The pressure bar is centrifugal apparatus, characterized in that the binding to the lower adapter body through the exposure hole.
The method of claim 15,

The lower adapter body member protrudes from an inner surface of the lower adapter body,

Centrifugal separator characterized in that the support bar for supporting the separating bag pressurized by the pressure bar is formed at the bottom.
The method of claim 8,

The adapter body,

A plurality of vertical incision bodies each including a horizontal movement preventing portion in contact with the inner surface of the rotating bucket, and a vertical support portion extending in the longitudinal direction of the rotating bucket integrally with the horizontal movement preventing portion,

When the vertical incision body is coupled to each other, the horizontal movement preventing portion at least a part of the inner surface of the rotating bucket to prevent the horizontal movement in the rotating bucket, the vertical support portion of both sides of the separation bag And close the fixing bag in the rotary bucket during the centrifugation process.
The method of claim 18,

The vertical incision body is formed of a frame in the form of a frame symmetric with each other,

The horizontal movement preventing unit corresponds to a horizontal frame in which both ends contact the inner surface of the rotating bucket at predetermined intervals in the longitudinal direction of the rotating bucket,

The vertical support portion, the centrifugal separator, characterized in that the vertical frame extending in the longitudinal direction of the rotating bucket to connect between the horizontal frame.
In the centrifuge bag used in the centrifugal separator,

It is formed by fusing at least one sheet of synthetic resin film formed using a synthetic resin, the injection hole located in the upper portion, the upper separation space in communication with the injection hole, the lower separation space for collecting the target material having a narrower width than the upper separation space, And a bag body providing an outlet for discharging the target substance collected in the lower separation space.

A fusion portion formed by fusion of the synthetic resin film around the bag body and formed along the shape of the adapter body to be fixed by the adapter body; And

A pressing member mounting hole formed at both sides of the lower separation space to enable the pressing member to be mounted;

Centrifuge bag comprising a.
The method of claim 20,

The bottom portion of the upper separation space and the side portion of the lower separation space has a structure narrowing toward the discharge port.
The method of claim 21,

The side portion of the lower separation space is a centrifugal bag, characterized in that the narrower than the bottom portion of the upper separation space to form a narrower angle.
The method of claim 20,

Centrifugal bag, characterized in that one or two or more of the pressing member mounting hole is formed symmetrically on both sides of the lower separation space.
The method of claim 21,

The fusion portion of the pressing member mounting hole adjacent to the outlet is formed in a relatively wider width than the surrounding of the other pressing member mounting hole.
The method of claim 1,

Centrifuge bag, characterized in that for forming a partition member for separating the upper and lower separation space in the bag body.