WO2010129283A3 - Microfluidic analyte capture using a thermoflowable material - Google Patents

Microfluidic analyte capture using a thermoflowable material Download PDF

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Publication number
WO2010129283A3
WO2010129283A3 PCT/US2010/032585 US2010032585W WO2010129283A3 WO 2010129283 A3 WO2010129283 A3 WO 2010129283A3 US 2010032585 W US2010032585 W US 2010032585W WO 2010129283 A3 WO2010129283 A3 WO 2010129283A3
Authority
WO
WIPO (PCT)
Prior art keywords
thermoflowable
channel
analyte
liquid
phase
Prior art date
Application number
PCT/US2010/032585
Other languages
French (fr)
Other versions
WO2010129283A2 (en
Inventor
Amit Gupta
Daniel Irimia
Mehmet Toner
Kenneth T. Kotz
Original Assignee
The General Hospital Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The General Hospital Corporation filed Critical The General Hospital Corporation
Publication of WO2010129283A2 publication Critical patent/WO2010129283A2/en
Publication of WO2010129283A3 publication Critical patent/WO2010129283A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The disclosure relates to the specific capture of an analyte such as a cell from a multi-component sample, such as whole blood. Microfluidic devices contain a channel with a solid phase thermoflowable material with a binding moiety to selectively bind the analyte from the sample. After removing unbound sample components from the channel, the bound analyte can be removed from the channel by converting the thermoflowable material to a liquid phase. The analyte can be detected in the liquid thermoflowable phase, or can be separated from the liquid thermoflowable material outside the channel.
PCT/US2010/032585 2009-04-27 2010-04-27 Microfluidic analyte capture using a thermoflowable material WO2010129283A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17294309P 2009-04-27 2009-04-27
US61/172,943 2009-04-27

Publications (2)

Publication Number Publication Date
WO2010129283A2 WO2010129283A2 (en) 2010-11-11
WO2010129283A3 true WO2010129283A3 (en) 2011-03-03

Family

ID=43050730

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/032585 WO2010129283A2 (en) 2009-04-27 2010-04-27 Microfluidic analyte capture using a thermoflowable material

Country Status (1)

Country Link
WO (1) WO2010129283A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10551376B2 (en) 2013-02-01 2020-02-04 The General Hospital Corporation Capture and release of particles from liquid samples

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5073341A (en) * 1985-08-21 1991-12-17 Biotope, Inc. Devices for conducting specific binding assays
US5637469A (en) * 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US20050244843A1 (en) * 2001-11-16 2005-11-03 Wen-Tien Chen Blood test prototypes and methods for the detection of circulating tumor and endothelial cells
US20070134713A1 (en) * 2002-04-25 2007-06-14 Bo Cao Methods and kits for detecting a target cell

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5073341A (en) * 1985-08-21 1991-12-17 Biotope, Inc. Devices for conducting specific binding assays
US5637469A (en) * 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US20050244843A1 (en) * 2001-11-16 2005-11-03 Wen-Tien Chen Blood test prototypes and methods for the detection of circulating tumor and endothelial cells
US20070134713A1 (en) * 2002-04-25 2007-06-14 Bo Cao Methods and kits for detecting a target cell

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ADAMS ET AL.: "Highly efficient circulating tumor cell isolating from whole blood and label free enumeration using polymer-based microfluidics with an integrated conductivity sensor", J AM CHEM SOC., vol. 130, 28 August 2008 (2008-08-28), pages 8633 - 8641, XP055097074, DOI: doi:10.1021/ja8015022 *

Also Published As

Publication number Publication date
WO2010129283A2 (en) 2010-11-11

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