WO2010054385A3 - Method and system for manufacture and use of macroporous beads in a multiplex assay - Google Patents

Method and system for manufacture and use of macroporous beads in a multiplex assay Download PDF

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Publication number
WO2010054385A3
WO2010054385A3 PCT/US2009/063899 US2009063899W WO2010054385A3 WO 2010054385 A3 WO2010054385 A3 WO 2010054385A3 US 2009063899 W US2009063899 W US 2009063899W WO 2010054385 A3 WO2010054385 A3 WO 2010054385A3
Authority
WO
WIPO (PCT)
Prior art keywords
beads
macroporous
multiplex assay
manufacture
macroporous beads
Prior art date
Application number
PCT/US2009/063899
Other languages
French (fr)
Other versions
WO2010054385A2 (en
Inventor
Jason Bedre
Don Chandler
Ben Mize
Original Assignee
Luminex Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Luminex Corporation filed Critical Luminex Corporation
Priority to EP09825598.7A priority Critical patent/EP2350651B1/en
Priority to CN200980144541.9A priority patent/CN102265155B/en
Publication of WO2010054385A2 publication Critical patent/WO2010054385A2/en
Publication of WO2010054385A3 publication Critical patent/WO2010054385A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
    • C08J9/26—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof by elimination of a solid phase from a macromolecular composition or article, e.g. leaching out
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
    • C09B67/006—Preparation of organic pigments
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
    • H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2325/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring; Derivatives of such polymers
    • C08J2325/02—Homopolymers or copolymers of hydrocarbons
    • C08J2325/04—Homopolymers or copolymers of styrene
    • C08J2325/06—Polystyrene

Abstract

Macroporous beads and a method of manufacturing and using such macroporous beads wherein the beads are distinguishable for use in a multiplex assay. Preferably, the beads are distinguishable by two or more unique fluorochromes, and at least some of the beads are magnetically responsive. In a preferred form, some of the macroporous beads have interior pores with a different moiety from the exterior surface, allowing beads with different attached functional groups.
PCT/US2009/063899 2008-11-10 2009-11-10 Method and system for manufacture and use of macroporous beads in a multiplex assay WO2010054385A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP09825598.7A EP2350651B1 (en) 2008-11-10 2009-11-10 Method for manufacture of macroporous beads
CN200980144541.9A CN102265155B (en) 2008-11-10 2009-11-10 Method and system for manufacture and use of macroporous beads in a multiplex assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11302708P 2008-11-10 2008-11-10
US61/113,027 2008-11-10

Publications (2)

Publication Number Publication Date
WO2010054385A2 WO2010054385A2 (en) 2010-05-14
WO2010054385A3 true WO2010054385A3 (en) 2010-09-10

Family

ID=42153651

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/063899 WO2010054385A2 (en) 2008-11-10 2009-11-10 Method and system for manufacture and use of macroporous beads in a multiplex assay

Country Status (4)

Country Link
US (2) US8877511B2 (en)
EP (1) EP2350651B1 (en)
CN (1) CN102265155B (en)
WO (1) WO2010054385A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8877511B2 (en) 2008-11-10 2014-11-04 Luminex Corporation Method and system for manufacture and use of macroporous beads in a multiplex assay
US20120021944A1 (en) * 2010-07-23 2012-01-26 Harold Baker Co-Coupling To Control Reactivity Of Reagents In Immunoassays
EP2989463B1 (en) 2013-04-26 2018-11-21 Bio-Rad Laboratories, Inc. Multiplex hepatitis b assay
US11320437B2 (en) * 2017-02-08 2022-05-03 Becton, Dickinson And Company Dried dye reagent devices and methods for making and using the same
US10401253B2 (en) * 2017-04-11 2019-09-03 Spectronics Corporation Fluorescent dye formulation and leak detection method
US11740240B2 (en) * 2020-07-20 2023-08-29 Bio-Rad Laboratories, Inc. Immunoassay for SARS-CoV-2 neutralizing antibodies and materials therefor

Citations (1)

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US20040126904A1 (en) * 1997-11-18 2004-07-01 Bio-Rad Laboratories, Inc. Multiplex flow assays preferably with magnetic particles as solid phase

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AU530410B2 (en) 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
NO155316C (en) 1982-04-23 1987-03-11 Sintef PROCEDURE FOR MAKING MAGNETIC POLYMER PARTICLES.
US5130343A (en) * 1991-03-13 1992-07-14 Cornell Research Foundation, Inc. Process for producing uniform macroporous polymer beads
US5168104A (en) * 1991-09-13 1992-12-01 Chembiomed, Ltd. Macroporous particles as biocompatible chromatographic supports
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
WO1997014028A2 (en) 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
JP4357112B2 (en) 1997-10-14 2009-11-04 ルミネックス コーポレイション Precise fluorescent dyed particles and methods for making and using the same
JP3468750B2 (en) 1998-01-22 2003-11-17 ルミネックス コーポレイション Microparticles with multiple fluorescent signals
JP4215397B2 (en) 1998-05-14 2009-01-28 ルミネックス コーポレイション Multiple analyte diagnostic system
DE60027576T2 (en) 1999-08-17 2007-05-03 Luminex Corp., Austin SECULATION OF FLUORESCENT PARTICLES
US6649414B1 (en) 1999-08-17 2003-11-18 Luminex Corporation Microparticles with multiple fluorescent signals and methods of using same
CA2387628C (en) 1999-10-18 2007-03-27 The Dial Corporation Apparatus and method for forming casingless sausages
KR100792021B1 (en) * 2000-03-30 2008-01-04 가부시키가이샤 에바라 세이사꾸쇼 Reactive probe chip and method for fabrication thereof
US6773812B2 (en) 2000-04-06 2004-08-10 Luminex Corporation Magnetically-responsive microspheres
DE10121903A1 (en) * 2001-04-27 2002-10-31 Nimbus Biotechnologie Gmbh System for membrane permeation measurement
US7241883B2 (en) 2001-11-14 2007-07-10 Luminex Corporation Functionalized compositions for improved immobilization
GB0216197D0 (en) * 2002-07-12 2002-08-21 Univ Strathclyde Serrs active particles
CN1219303C (en) * 2003-05-15 2005-09-14 中国科学院化学研究所 Method for preparing magnetic nano microparticles with biological compatibility
KR20070086095A (en) 2004-11-17 2007-08-27 루미넥스 코포레이션 Device and method for managing data from a flow analyzer
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EP3118333B1 (en) * 2006-12-13 2019-04-03 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
US8877511B2 (en) 2008-11-10 2014-11-04 Luminex Corporation Method and system for manufacture and use of macroporous beads in a multiplex assay

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040126904A1 (en) * 1997-11-18 2004-07-01 Bio-Rad Laboratories, Inc. Multiplex flow assays preferably with magnetic particles as solid phase
US20060269962A1 (en) * 1997-11-18 2006-11-30 Bio-Rad Laboratories, Inc. Multiplex flow assays preferably with magnetic particles as solid phase
US20060275820A1 (en) * 1997-11-18 2006-12-07 Bio-Rad Laboratories, Inc. Multiplex flow assays preferably with magnetic particles as solid phase

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KIM J.-W. ET AL.: "Monodisperse micron-sized macroporous poly(styrene-co-div inylbenzene) particles by seeded polymerization", COLLOID POLYM SCI, vol. 279, 2001, pages 146 - 152, XP008147702 *

Also Published As

Publication number Publication date
CN102265155A (en) 2011-11-30
WO2010054385A2 (en) 2010-05-14
EP2350651B1 (en) 2015-01-14
CN102265155B (en) 2014-08-13
US9745438B2 (en) 2017-08-29
US20150041703A1 (en) 2015-02-12
EP2350651A2 (en) 2011-08-03
EP2350651A4 (en) 2012-06-06
US20100120165A1 (en) 2010-05-13
US8877511B2 (en) 2014-11-04

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