WO2009129524A3 - High-throughput plasmid dna preparation - Google Patents

High-throughput plasmid dna preparation Download PDF

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Publication number
WO2009129524A3
WO2009129524A3 PCT/US2009/041087 US2009041087W WO2009129524A3 WO 2009129524 A3 WO2009129524 A3 WO 2009129524A3 US 2009041087 W US2009041087 W US 2009041087W WO 2009129524 A3 WO2009129524 A3 WO 2009129524A3
Authority
WO
WIPO (PCT)
Prior art keywords
plasmid dna
dna preparation
throughput
throughput plasmid
provides
Prior art date
Application number
PCT/US2009/041087
Other languages
French (fr)
Other versions
WO2009129524A2 (en
Inventor
Frederick M. Boyce
Original Assignee
The General Hospital Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The General Hospital Corporation filed Critical The General Hospital Corporation
Publication of WO2009129524A2 publication Critical patent/WO2009129524A2/en
Publication of WO2009129524A3 publication Critical patent/WO2009129524A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Abstract

The present invention provides for compositions and methods of high-throughput plasmid DNA purification from E. coli bacteria. More specifically, an acidic, zwitterionic detergent solution provides for superior removal of contaminating endotoxins and improves transfectability of plasmid DNA.
PCT/US2009/041087 2008-04-18 2009-04-20 High-throughput plasmid dna preparation WO2009129524A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4618608P 2008-04-18 2008-04-18
US61/046,186 2008-04-18

Publications (2)

Publication Number Publication Date
WO2009129524A2 WO2009129524A2 (en) 2009-10-22
WO2009129524A3 true WO2009129524A3 (en) 2009-12-17

Family

ID=41199786

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/041087 WO2009129524A2 (en) 2008-04-18 2009-04-20 High-throughput plasmid dna preparation

Country Status (1)

Country Link
WO (1) WO2009129524A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112322612A (en) * 2020-10-29 2021-02-05 江苏凯基生物技术股份有限公司 Plasmid extraction kit and extraction method
CN117751186A (en) 2021-08-05 2024-03-22 默克专利股份公司 Methods for reducing endotoxin levels for nucleic acid purification
WO2023170028A1 (en) 2022-03-10 2023-09-14 Merck Patent Gmbh Method for reducing endotoxin levels in nucleic acid purification

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
US6313285B1 (en) * 1999-07-23 2001-11-06 Genentech, Inc. Purification of plasmid DNA
US20040180445A1 (en) * 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
US20070015169A1 (en) * 2005-07-16 2007-01-18 Xiyu Jia Isolation of nucleic acid using colored buffers

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
US6313285B1 (en) * 1999-07-23 2001-11-06 Genentech, Inc. Purification of plasmid DNA
US20040180445A1 (en) * 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
US20070015169A1 (en) * 2005-07-16 2007-01-18 Xiyu Jia Isolation of nucleic acid using colored buffers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
E.H. CHOWDHURY ET AL., JOURNAL OF BIOTECHNOLOGY, vol. 119, 2005, pages 343 - 347 *

Also Published As

Publication number Publication date
WO2009129524A2 (en) 2009-10-22

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