WO2009086525A4 - Luminescent reporter modality for analyzing an assay - Google Patents
Luminescent reporter modality for analyzing an assay Download PDFInfo
- Publication number
- WO2009086525A4 WO2009086525A4 PCT/US2008/088465 US2008088465W WO2009086525A4 WO 2009086525 A4 WO2009086525 A4 WO 2009086525A4 US 2008088465 W US2008088465 W US 2008088465W WO 2009086525 A4 WO2009086525 A4 WO 2009086525A4
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- luminescent
- optical analysis
- assay
- light
- particles
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0346—Capillary cells; Microcells
Abstract
An optical analysis flow system and a method of assay analysis includes a means for facilitating activation of a luminescent material coupled to particles entrained within a fluid assay, wherein the means is arranged such that the activation of the luminescent material is conducted at a site along a flow path of the fluid assay prior to an examination zone of the optical analysis flow system. The method further includes measuring luminescent light emitting from the particles as they flow through the examination zone. Another method of assay analysis includes respectively measuring different types of luminescent light emission from a first set and a second set of particles comprising a fluid assay. An optical analysis system includes at least two distinct means for respectively facilitating the activation of at least two different luminescent materials coupled to particles of a fluid assay.
Claims
AMENDED CLAIMS received by the International Bureau on 30 September 2009 (30.09.2009)
WHAT IS CLAIMED IS:
1. A method for analyzing an assay within an optical analysis flow system, comprising: injecting a fluid assay into an optical analysis flow system, wherein the fluid assay comprises a first subset of particles coupled with a non-fluorescent luminescent compound and a second subset of particles coupled with a fluorescent compound; activating the non- fluorescent luminescent compound on at Jeast some of the particles within the optical analysis flow system at a site along a flow path of the fluid assay prior to an examination zone of the optical analysis flow system such that the particles coupled with the activated luminescent compound emit luminescent light wilhτn the examination zone; measuring the luminescent light emitting from the particles coupled with the activated luminescent compound as they flow through the examination zone; identifying and quantifying a first analytc of interest based on the measured luminescent light; illuminating the examination zone with a light source; measuring fluorescent light emitting from the particles coupled with the fluorescent compound as they flow through the examination zone; and identifying and quantifying a second analyte of interest based on the measured fluorescent light.
2. The method of claim I, wherein the non-fluorescent luminescent compound is a cliemi luminescent compound, wherein the step of activating the cheniiluminescent compound comprises introducing an activation reagent into the flow path of the fluid assay, and wherein the activation reagent is configured to react with the chcmiluminescent compound to generate luminescent light.
3. The method of claim 2, wherein the cheniiluminesccnt compound is an acridiπium compound.
4. The method of claim I, wherein lhc step of measuring the luminescent light comprises measuring luminescent light emitting from a given particle less than approximately 500 milliseconds after the luminescent compound on the given particle is activated.
6. The method of claim 1 , further comprising selectively blocking or turning off the light source during the step of measuring the luminescent light emitted from the particles coupled wilh the non-fluorescent luminescent compound.
7. An optical analysis flow system, comprising: an interrogation flow cell; . a fluid handling system comprising: a sheath Ωuidic line for supplying a sheath fluid into tlic interrogation flow cell; and an assay fluidic line extending into lhc interrogation flow cell for introducing a fluid assay into a flow of the sheath fluid within the interrogation flow cell; and a means for facilitating activation of a luminescent material coupled to particles entrained, within the fluid assay, wherein the means for facilitating activation of the luminescent material is arranged such that the aclivalion of the luminescent material is conducted ai a site along a flow path of the fluid assay prior to an examination zone of the interrogation flow cell; an illumination system configured to illuminate the examination zone of the interrogation flow cell; and a control system comprising a processor and program instructions executable by the processor wherein the control system further comprises program instructions executable by the processor for selectively blocking or turning off a light source of the illumination system in conjunction with selectively employing the means for facilitating activation of the luminescent material.
8. The optical analysis flow system of claim 7, wherein the means for facilitating activation of the luminescent material is arranged such thai the site of activation is less than approximately 1 .0 inch from Lhc examination zone of the interrogation flow cell.
9. The optical analysis flow system of claim 7, wherein the means for facilitating activation of the luminescent material comprises a reagent fluidic line arranged relative to the assay fluidic line for adding a reagent to the fluid assay prior to the examination zone, wherein the reagent is configured to react with the luminescent material to generate luminescent light.
10. The optical analysis flow system of claim 9, wherein the means for facilitating activation of the luminescent material further includes a three-way valve comprising: an outlet port coupled to iho assay fluidic line; an assay inlet port coupled to an assay inlet line for introducing the fluid assay into the assay fluidic line; and a reagent inlet port coupled Io the reagent fluidic line for introducing the reagent into the assay fluidic line.
12. The optical analysis flow system of chum 7, further comprising: a detection system configured to collect light emitted and/or scattered from particles passing through the examination zone and further configured to generate signals representative of a degree of light gathered; and an examination system for analyzing the generated signals, wherein the examination system comprises program instructions executable by a processor for associating a generated signal to a.particular analyte of interest based on whether the means for facilitating activation of the luminescent material is employed.
13. The optical analysis flow system of claim 7, further comprising: n detection system configured to collect light emitted and/or scattered from particles passing through the examination zone and further configured to generate signals representative of a degree of light gathered; and tin examination system for analyzing the generated signals, wherein the examination system comprises program instructions executable by a processor for associating a generated signal Io a particular analyte of interest based on whether the light source is blocked and/or turned off when the light corresponding to the generated signals is collected.
14. The optical analysis flow system of claim 7, further comprising: a detection system comprising distinct detectors spaced along the examination zone for respectively collecting different types of luminescent light emissions, wherein each of the distinct detectors is configured to generate signals representative of a degree of light gathered; and an examination system for analyzing the generated signals, wherein the examination systcm comprises program instructions executable by a processor for associating a generated signal to a particular analytc of interest based on which of the distinct detectors generated the signal.
15. Λ method for analyzing a fluid assay, comprising: introducing a fluid assay into an optical analysis system; measuring a first type of luminescent light emission from a first set of particles comprising the fluid assay; identifying and quantifying a first analytc of interest based on the first type of luminescent light; measuring a second distinct type of luminescent light emission from a second set of particles comprising the fluid assay; and identifying and quantifying a second analyte of interest based on the second distinct type of luminescent light.
16. The method of claim 15, wherein the steps of measuring the first and second types of luminescent light emissions are performed sequentially,
17. The method of claim 15, wherein the steps of measuring the first and second types of luminescent light emissions arc performed concurrently.
18. The method of claim 15, wherein the steps of measuring the first and second types of luminescent light emissions are alternately performed.
19. The method of claim 15, wherein the optical analysis system is an optical analysis flow system.
20. The method of claim 15, wherein the optical analysis system is a static imaging oplical analysis system,
21. The method of claim 15, wherein the first aαd second types ofliimincsccnt light emissions arc selected from the group consisting of phololiiniincsccnce, fluorescence, phosphorescence, chemi luminescence, biohiminescence, crysiallolumincsccnce, electroluminescence, cathodolummescencc, mechanolumncscence, iriboluminescence, fractoluminescence, piezoluminiscence , radioluminescence, sonoluminescence, and thermoluminescence,
22. The method of claim 15, wherein the step of measuring the first type of luminescent light emission comprises measuring fluorescent light emission, and wherein the step of measuring the second distinct type of luminescent light emission comprises measuring chemiluminescent light emission.
23. An optical analysis system, comprising: a particle examination chamber; an assay fluidic line distinct from the particle examination chamber, but operably coupled to the particle examination chamber such that particles entrained within a fluid assay flowing in the assay fluidic line are routed to the particle examination chamber; and at least two distinct means for respectively facilitating the activation of at least two different luminescent materials coupled to the particles.
24. Tlic optical analysis system of claim 23, further comprising a control system comprising a processor and program instructions executable by the processor for selectively employing lhc distinct means for facilitating activation of the different luminescent materials.
25. The oplical analysis system of claim 23, wherein at least one means for facilitating activation of one of the luminescent materials comprises a reagent fluidic line for adding a reagent to the particles, wherein the reagent is configured to react with the luminescent material to generate luminescent light,
26. The optical analysis system of claim 23, wherein at least one means for facilitaling activation of the luminescent materials comprises an illumination system.
27. The optical analysis system of claim 23, wherein the optical analysis system is an optical analysis flow system.
28. The optical analysis system of claim 27, wherein al least one of the means for facilitating, the activation of one ofthc luminescent materials is configured such that the activation of the one luminescent material is conducted at a site along a flow path of the fluid assay prior to the particle examination chamber.
29. The optical analysis system of claim 23, wherein the optical analysis system is static imaging analysis system.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1687907P | 2007-12-27 | 2007-12-27 | |
| US61/016,879 | 2007-12-27 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO2009086525A2 WO2009086525A2 (en) | 2009-07-09 |
| WO2009086525A3 WO2009086525A3 (en) | 2009-09-24 |
| WO2009086525A4 true WO2009086525A4 (en) | 2009-11-19 |
Family
ID=40798940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2008/088465 WO2009086525A2 (en) | 2007-12-27 | 2008-12-29 | Luminescent reporter modality for analyzing an assay |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US20090170214A1 (en) |
| WO (1) | WO2009086525A2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102010054879B4 (en) * | 2010-12-17 | 2013-07-18 | Institut für Bioprozess- und Analysenmesstechnik e.V. | Arrangement and method for conditioning fluid compartments |
| US9395308B2 (en) * | 2013-10-10 | 2016-07-19 | University Of Akron | Apparatus for quantitative measurements of stress distributions from mechanoluminescene materials |
| US10585028B2 (en) | 2017-10-20 | 2020-03-10 | Charted Scientific, Inc. | Method and apparatus for optical analysis |
| US11041756B2 (en) | 2017-10-20 | 2021-06-22 | Charted Scientific Inc. | Method and apparatus of filtering light using a spectrometer enhanced with additional spectral filters with optical analysis of fluorescence and scattered light from particles suspended in a liquid medium using confocal and non confocal illumination and imaging |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DE2943116C2 (en) * | 1979-10-25 | 1986-06-19 | Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München | Device for flow cytometric reaction and / or diffusion measurement |
| US4573796A (en) * | 1984-01-06 | 1986-03-04 | The United States Of America As Represented By The United States Department Of Energy | Apparatus for eliminating background interference in fluorescence measurements |
| US5032381A (en) * | 1988-12-20 | 1991-07-16 | Tropix, Inc. | Chemiluminescence-based static and flow cytometry |
| US5981180A (en) * | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| WO1997014028A2 (en) * | 1995-10-11 | 1997-04-17 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| US5736330A (en) * | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
| US6449562B1 (en) * | 1996-10-10 | 2002-09-10 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| EP1021566A4 (en) * | 1997-06-09 | 2002-06-19 | Caliper Techn Corp | Apparatus and methods for correcting for variable velocity in microfluidic systems |
| HUP0003986A3 (en) * | 1997-10-14 | 2001-04-28 | Luminex Corp Austin | Precision fluorescently dyed particles and methods of making and using same |
| ATE239801T1 (en) * | 1998-01-22 | 2003-05-15 | Luminex Corp | MICROPARTICLES WITH MULTIPLE FLUORESCENCE SIGNALS |
| CA2331896C (en) * | 1998-05-14 | 2009-09-15 | Luminex Corporation | Diode laser based measurement apparatus |
| WO1999058955A1 (en) * | 1998-05-14 | 1999-11-18 | Luminex Corporation | Multi-analyte diagnostic system and computer implemented process for same |
| JP3946444B2 (en) * | 1998-05-14 | 2007-07-18 | ルミネックス コーポレイション | Configuration and method for zero dead time of flow cytometer |
| US6315952B1 (en) * | 1998-10-05 | 2001-11-13 | The University Of New Mexico | Plug flow cytometry for high throughput screening and drug discovery |
| DE60040603D1 (en) * | 1999-08-17 | 2008-12-04 | Luminex Corp | METHOD FOR ANALYZING A MULTIPLE OF SAMPLES OF VARIOUS ORIGIN ON AN ANALYTE |
| EP1208382B1 (en) * | 1999-08-17 | 2006-04-26 | Luminex Corporation | Encapsulation of fluorescent particles |
| US6949377B2 (en) * | 2001-03-05 | 2005-09-27 | Ho Winston Z | Chemiluminescence-based microfluidic biochip |
| US20030113714A1 (en) * | 2001-09-28 | 2003-06-19 | Belcher Angela M. | Biological control of nanoparticles |
| CN101072992A (en) * | 2004-11-12 | 2007-11-14 | 卢米尼克斯股份有限公司 | Methods and systems for positioning microspheres for imaging |
| US20060228720A1 (en) * | 2005-04-12 | 2006-10-12 | Eastman Kodak Company | Method for imaging an array of microspheres |
| CA2984777C (en) * | 2005-09-21 | 2018-04-03 | Luminex Corporation | Methods and systems for image data processing |
| KR101452295B1 (en) * | 2006-06-02 | 2014-10-21 | 루미넥스 코포레이션 | Systems and methods for performing measurements of one or more analytes comprising using magnetic particles and applying a magnetic field |
| WO2008061058A2 (en) * | 2006-11-10 | 2008-05-22 | Luminex Corporation | Flow cytometer and fluidic line assembly with multiple injection needles |
-
2008
- 2008-12-29 WO PCT/US2008/088465 patent/WO2009086525A2/en active Application Filing
- 2008-12-29 US US12/345,285 patent/US20090170214A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009086525A2 (en) | 2009-07-09 |
| WO2009086525A3 (en) | 2009-09-24 |
| US20090170214A1 (en) | 2009-07-02 |
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