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Publication numberWO2009083648 A3
Publication typeApplication
Application numberPCT/FI2008/050781
Publication date3 Sep 2009
Filing date22 Dec 2008
Priority date27 Dec 2007
Also published asDE112008003552T5, DE112008003552T9, WO2009083648A2
Publication numberPCT/2008/50781, PCT/FI/2008/050781, PCT/FI/2008/50781, PCT/FI/8/050781, PCT/FI/8/50781, PCT/FI2008/050781, PCT/FI2008/50781, PCT/FI2008050781, PCT/FI200850781, PCT/FI8/050781, PCT/FI8/50781, PCT/FI8050781, PCT/FI850781, WO 2009/083648 A3, WO 2009083648 A3, WO 2009083648A3, WO-A3-2009083648, WO2009/083648A3, WO2009083648 A3, WO2009083648A3
InventorsDavid A. Cohen, Michael J. Mortillaro, Charles Patrick André
ApplicantFinnzymes Instruments Oy
Export CitationBiBTeX, EndNote, RefMan
External Links: Patentscope, Espacenet
Thermal cycling instrument and methods for carrying out pcr
WO 2009083648 A3
Abstract
The invention relates to a polymerase chain reaction (PCR) process and a thermal cycler. In the process, biological samples are held in a sample carrier (10) having a plurality of sample spaces (12) each having upper and lower ends and the samples are sequentially heated and cooled. The thermal cycler according to the invention comprises heat transfer means for automatic heating and cooling of the samples in the sample carrier, heatable closure means (18) above the upper ends of the samples spaces (12) for preventing condensation of sample vapor during the process, and means for adjusting one process parameter, preferably the temperature of the heatable closure means, depending on at least one other of said process parameters. The invention helps to decrease the number of failed PCR experiments in particular due to changes in sample volume.
Non-Patent Citations
Reference
1 *BIO-RAD LABORATORIES, INC.: "MJ Mini Gradient Thermal Cycler. Operations Manual"[Online] 2005, pages 1-96, XP002530917 Retrieved from the Internet: URL:http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_10968.pdf> [retrieved on 2009-06-14]
2 *FINNZYMES INSTRUMENT: "Piko Thermal Cycler. User Manual."[Online] October 2007 (2007-10), pages 1-14, XP002530918 Retrieved from the Internet: URL:http://www.finnzymes.com/pdf/piko_thermal_cycler_usermanual.pdf> [retrieved on 2009-06-14]
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US825258122 Jan 200828 Aug 2012Wafergen, Inc.Apparatus for high throughput chemical reactions
US913242724 Aug 201215 Sep 2015Wafergen, Inc.Apparatus for high throughput chemical reactions
US922893323 Nov 20095 Jan 2016Wafergen, Inc.Apparatus and method for multiplex analysis
Classifications
International ClassificationB01L7/00
Cooperative ClassificationB01L2200/142, B01L3/50853, B01L7/52, B01L3/50851, B01L2300/046, B01L2300/0829
European ClassificationB01L3/50851, B01L3/50853, B01L7/52
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