WO2009054870A3 - Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples - Google Patents

Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples Download PDF

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Publication number
WO2009054870A3
WO2009054870A3 PCT/US2008/008640 US2008008640W WO2009054870A3 WO 2009054870 A3 WO2009054870 A3 WO 2009054870A3 US 2008008640 W US2008008640 W US 2008008640W WO 2009054870 A3 WO2009054870 A3 WO 2009054870A3
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WO
WIPO (PCT)
Prior art keywords
biological samples
nucleic acid
acid extraction
diagnostic testing
multiple biological
Prior art date
Application number
PCT/US2008/008640
Other languages
French (fr)
Other versions
WO2009054870A2 (en
Inventor
Jeff Williams
Kerry Wilson
Kalyan Handique
Original Assignee
Handylab Inc
Jeff Williams
Kerry Wilson
Kalyan Handique
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40580274&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2009054870(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EP16191773.7A priority Critical patent/EP3178558B1/en
Priority to PL16191787T priority patent/PL3178559T3/en
Priority to EP16191787.7A priority patent/EP3178559B1/en
Priority to CN201810170491.2A priority patent/CN108165462B/en
Priority to ES08843060.8T priority patent/ES2610128T3/en
Priority to EP22164041.0A priority patent/EP4101537A3/en
Priority to AU2008317492A priority patent/AU2008317492B2/en
Application filed by Handylab Inc, Jeff Williams, Kerry Wilson, Kalyan Handique filed Critical Handylab Inc
Priority to CA2698253A priority patent/CA2698253C/en
Priority to EP08843060.8A priority patent/EP2179025B1/en
Priority to CN2008801067603A priority patent/CN101802164B/en
Priority to EP16191793.5A priority patent/EP3144065B1/en
Priority to JP2010517005A priority patent/JP5261485B2/en
Priority to DK08843060.8T priority patent/DK2179025T3/en
Publication of WO2009054870A2 publication Critical patent/WO2009054870A2/en
Publication of WO2009054870A3 publication Critical patent/WO2009054870A3/en

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    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
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    • B01L3/502738Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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    • C12Q1/6844Nucleic acid amplification reactions
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    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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    • G01N2021/6441Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
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Abstract

The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.
PCT/US2008/008640 2007-07-13 2008-07-14 Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples WO2009054870A2 (en)

Priority Applications (13)

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DK08843060.8T DK2179025T3 (en) 2007-07-13 2008-07-14 Integrated apparatus for performing nucleic acid extraction and diagnostic tests of multiple biological samples
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