WO2009019292A3 - Apparatus for cytofluorimetric analyses and methods for optimizing the conditioning chain - Google Patents

Apparatus for cytofluorimetric analyses and methods for optimizing the conditioning chain Download PDF

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Publication number
WO2009019292A3
WO2009019292A3 PCT/EP2008/060357 EP2008060357W WO2009019292A3 WO 2009019292 A3 WO2009019292 A3 WO 2009019292A3 EP 2008060357 W EP2008060357 W EP 2008060357W WO 2009019292 A3 WO2009019292 A3 WO 2009019292A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
optimizing
analyses
conditioning chain
cytofluorimetric
Prior art date
Application number
PCT/EP2008/060357
Other languages
French (fr)
Other versions
WO2009019292A2 (en
Inventor
Giuseppe Gigli
Marco Esposito
Original Assignee
Consiglio Nazionale Ricerche
Giuseppe Gigli
Marco Esposito
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consiglio Nazionale Ricerche, Giuseppe Gigli, Marco Esposito filed Critical Consiglio Nazionale Ricerche
Publication of WO2009019292A2 publication Critical patent/WO2009019292A2/en
Publication of WO2009019292A3 publication Critical patent/WO2009019292A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1429—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths

Abstract

There is disclosed an apparatus for cytofluorimetric analyses including an optical system, based on at least one multi-channel photomultiplier allowing to condition the signals exclusively in the digital domain by means of a conditioning chain formed by routine software, the specific optimal operation parameters of which are obtained by means of methods which are also an object of the present invention, thus obtaining an efficiency which is so high that it allows more reliable and detailed analyses than in the past.
PCT/EP2008/060357 2007-08-06 2008-08-06 Apparatus for cytofluorimetric analyses and methods for optimizing the conditioning chain WO2009019292A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITMI20071632 ITMI20071632A1 (en) 2007-08-06 2007-08-06 APPARATUS FOR AFTERFLOWING INVESTIGATIONS AND METHODS OF OPTIMIZATION OF THE CONDITIONING CHAIN
ITMI2007A001632 2007-08-06

Publications (2)

Publication Number Publication Date
WO2009019292A2 WO2009019292A2 (en) 2009-02-12
WO2009019292A3 true WO2009019292A3 (en) 2009-05-22

Family

ID=40042876

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/060357 WO2009019292A2 (en) 2007-08-06 2008-08-06 Apparatus for cytofluorimetric analyses and methods for optimizing the conditioning chain

Country Status (2)

Country Link
IT (1) ITMI20071632A1 (en)
WO (1) WO2009019292A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105606381B (en) * 2016-01-28 2017-12-26 同济大学 A kind of Distributed filtering network system and design method

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5150313A (en) * 1990-04-12 1992-09-22 Regents Of The University Of California Parallel pulse processing and data acquisition for high speed, low error flow cytometry
WO1996034270A2 (en) * 1995-04-25 1996-10-31 Systemix Fluorescence spectral overlap compensation for high speed flow cytometry systems
US5690105A (en) * 1995-07-04 1997-11-25 Toa Medical Electronics Co., Ltd. Method and apparatus for determining a particle criterion and particle analyzer using the criterion
US6221671B1 (en) * 1997-12-12 2001-04-24 Chemunex S.A. Digital flow cytometer and method
US20030098422A1 (en) * 2001-11-07 2003-05-29 Silcott David B. System and method for detecting and classifying biological particles
US20050057749A1 (en) * 1998-08-21 2005-03-17 Surromed, Inc. Novel optical architectures for microvolume laser-scanning cytometers
US20050104008A1 (en) * 2001-08-28 2005-05-19 Oostman Clifford A.Jr. Fluorescence detection instrument with reflective transfer legs for color decimation
US20050275839A1 (en) * 2004-04-08 2005-12-15 Robinson Joseph P Multi-spectral detector and analysis system

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5150313A (en) * 1990-04-12 1992-09-22 Regents Of The University Of California Parallel pulse processing and data acquisition for high speed, low error flow cytometry
WO1996034270A2 (en) * 1995-04-25 1996-10-31 Systemix Fluorescence spectral overlap compensation for high speed flow cytometry systems
US5690105A (en) * 1995-07-04 1997-11-25 Toa Medical Electronics Co., Ltd. Method and apparatus for determining a particle criterion and particle analyzer using the criterion
US6221671B1 (en) * 1997-12-12 2001-04-24 Chemunex S.A. Digital flow cytometer and method
US20050057749A1 (en) * 1998-08-21 2005-03-17 Surromed, Inc. Novel optical architectures for microvolume laser-scanning cytometers
US20050104008A1 (en) * 2001-08-28 2005-05-19 Oostman Clifford A.Jr. Fluorescence detection instrument with reflective transfer legs for color decimation
US20030098422A1 (en) * 2001-11-07 2003-05-29 Silcott David B. System and method for detecting and classifying biological particles
US20050275839A1 (en) * 2004-04-08 2005-12-15 Robinson Joseph P Multi-spectral detector and analysis system

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CYTOMETRY, vol. 57A, no. 2, February 2004 (2004-02-01), pages 63 - 69, ISSN: 0196-4763 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; February 2004 (2004-02-01), SNOW CHRISTOPHER "KIT": "Flow cytometer electronics.", XP002519272, Database accession no. PREV200400143479 *
MURTHI S ET AL: "Improved data acquisition system for digital flow cytometry", ISCAS 2002. PROCEEDINGS OF THE 2002 IEEE INTERNATIONAL SYMPOSIUM ON CIRCUITS AND SYSTEMS. SCOTTSDALE, AZ, MAY 26 - 29, 2002; [IEEE INTERNATIONAL SYMPOSIUM ON CIRCUITS AND SYSTEMS], NEW YORK, NY : IEEE, US, vol. 1, 26 May 2002 (2002-05-26), pages I/669 - I/672, XP002507222, ISBN: 978-0-7803-7448-5 *
MURTHI SHIVA ET AL: "Performance analysis of a dual-buffer architecture for digital flow cytometry", CYTOMETRY, vol. 66A, no. 2, August 2005 (2005-08-01), pages 109 - 118, XP002519271, ISSN: 1552-4922(print) 1552-4930(ele *
STARLING D ET AL: "COMPENSATION OF FLUORESCENCE CROSSTALK BY DIGITAL SIGNAL PROCESSING", CYTOMETRY, ALAN LISS, NEW YORK, US, no. SUPPL. 09, 1 January 1998 (1998-01-01), pages 119, XP009026463, ISSN: 0196-4763 *

Also Published As

Publication number Publication date
ITMI20071632A1 (en) 2009-02-07
WO2009019292A2 (en) 2009-02-12

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