WO2008139421A3 - Electrical transduction method and device for the detection of biorecognition events in biomolecular interaction processes for genome/proteome analysis - Google Patents

Electrical transduction method and device for the detection of biorecognition events in biomolecular interaction processes for genome/proteome analysis Download PDF

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Publication number
WO2008139421A3
WO2008139421A3 PCT/IB2008/051900 IB2008051900W WO2008139421A3 WO 2008139421 A3 WO2008139421 A3 WO 2008139421A3 IB 2008051900 W IB2008051900 W IB 2008051900W WO 2008139421 A3 WO2008139421 A3 WO 2008139421A3
Authority
WO
WIPO (PCT)
Prior art keywords
biorecognition
electrodes
conductive
nanojunction
interact
Prior art date
Application number
PCT/IB2008/051900
Other languages
French (fr)
Other versions
WO2008139421A2 (en
Inventor
Giuseppe Maruccio
Elisabetta Primiceri
Pasquale Marzo
Valentina Arima
Roman Krahne
Teresa Pellegrino
Torre Antonio Della
Franco Calabi
Roberto Cingolani
Rosaria Rinaldi
Original Assignee
Consiglio Nazionale Ricerche
Fond Istituto Italiano Di Tecn
Giuseppe Maruccio
Elisabetta Primiceri
Pasquale Marzo
Valentina Arima
Roman Krahne
Teresa Pellegrino
Torre Antonio Della
Franco Calabi
Roberto Cingolani
Rosaria Rinaldi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consiglio Nazionale Ricerche, Fond Istituto Italiano Di Tecn, Giuseppe Maruccio, Elisabetta Primiceri, Pasquale Marzo, Valentina Arima, Roman Krahne, Teresa Pellegrino, Torre Antonio Della, Franco Calabi, Roberto Cingolani, Rosaria Rinaldi filed Critical Consiglio Nazionale Ricerche
Publication of WO2008139421A2 publication Critical patent/WO2008139421A2/en
Publication of WO2008139421A3 publication Critical patent/WO2008139421A3/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
    • H10K10/00—Organic devices specially adapted for rectifying, amplifying, oscillating or switching; Organic capacitors or resistors having a potential-jump barrier or a surface barrier
    • H10K10/701—Organic molecular electronic devices
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
    • H10K85/00—Organic materials used in the body or electrodes of devices covered by this subclass
    • H10K85/761—Biomolecules or bio-macromolecules, e.g. proteins, chlorophyl, lipids or enzymes

Abstract

A method is described for detecting biorecognition events in biomolecular interaction processes, including the provision, on at least one biorecognition site, of molecular probes (P) adapted to interact with target molecular species (T) which are coupled to conductive nanoparticles (NP), in which the probes (P) are associated with a nanojunction transducer device (12) comprising a pair of conductive electrodes (22, 24) separated by a nanometric gap of the same order of magnitude as the dimensions of said conductive nanoparticles (NP), adapted to detect a condition of coupling between at least one molecular probe (P) and a specimen of a target species (T) which interact with each other. A biorecognition event includes the coupling of a probe (P) to at least one specimen of the target species (T) and results in the positioning of the conductive nanoparticle (NP) coupled to the target species (T) (by being directly conjugated with the latter or with a signal molecule which can interact with it) between the electrodes (22, 24) of the nanojunction device (12), with the consequent creation of a conductive path between the electrodes. The occurrence of biorecognition events is evaluated according to the intensity of the current which is made to flow between the electrodes (22, 24) of the nanojunction device (12) by the application of a potential difference.
PCT/IB2008/051900 2007-05-15 2008-05-14 Electrical transduction method and device for the detection of biorecognition events in biomolecular interaction processes for genome/proteome analysis WO2008139421A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITTO2007A000341 2007-05-15
IT000341A ITTO20070341A1 (en) 2007-05-15 2007-05-15 PROCEDURE AND DEVICE FOR ELECTRIC TRANSDUCTION FOR THE DETECTION OF BIO-RECOGNITION EVENTS IN BIOMOLECULAR INTERACTION PROCESSES FOR GENOMIC / PROTEOMIC ANALYSIS

Publications (2)

Publication Number Publication Date
WO2008139421A2 WO2008139421A2 (en) 2008-11-20
WO2008139421A3 true WO2008139421A3 (en) 2009-03-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/051900 WO2008139421A2 (en) 2007-05-15 2008-05-14 Electrical transduction method and device for the detection of biorecognition events in biomolecular interaction processes for genome/proteome analysis

Country Status (2)

Country Link
IT (1) ITTO20070341A1 (en)
WO (1) WO2008139421A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7174065B2 (en) * 2018-04-17 2022-11-17 コリア リサーチ インスティテュート オブ ケミカル テクノロジー Multiwell electrode-based biosensor

Citations (8)

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Publication number Priority date Publication date Assignee Title
US5191784A (en) * 1989-12-28 1993-03-09 Siemens Corporate Research, Inc. Opto-electronic gas sensor
WO2001044501A2 (en) * 1999-12-13 2001-06-21 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Method and device for detecting and quantifying biomolecules
WO2001073123A2 (en) * 2000-03-28 2001-10-04 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
EP1231214A2 (en) * 2001-02-01 2002-08-14 Hitachi Software Engineering Co., Ltd. Biopolymer detector
WO2004036217A1 (en) * 2002-10-12 2004-04-29 Fujitsu Limited Semiconductor base structure for molecular electronics and molecular electronic-based biosensor devices and a method for producing such a semiconductor base structure
WO2005008450A2 (en) * 2003-03-28 2005-01-27 The Regents Of The University Of California Method and apparatus for nanogap device and array
WO2005108612A2 (en) * 2003-11-28 2005-11-17 Genorx, Inc. Nanoscale biosensor device, system and technique
WO2006067276A1 (en) * 2004-12-23 2006-06-29 Nanolab Systems Oy Utilization of nanowires in chemical and biological analysis

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5191784A (en) * 1989-12-28 1993-03-09 Siemens Corporate Research, Inc. Opto-electronic gas sensor
WO2001044501A2 (en) * 1999-12-13 2001-06-21 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Method and device for detecting and quantifying biomolecules
WO2001073123A2 (en) * 2000-03-28 2001-10-04 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
EP1231214A2 (en) * 2001-02-01 2002-08-14 Hitachi Software Engineering Co., Ltd. Biopolymer detector
WO2004036217A1 (en) * 2002-10-12 2004-04-29 Fujitsu Limited Semiconductor base structure for molecular electronics and molecular electronic-based biosensor devices and a method for producing such a semiconductor base structure
WO2005008450A2 (en) * 2003-03-28 2005-01-27 The Regents Of The University Of California Method and apparatus for nanogap device and array
WO2005108612A2 (en) * 2003-11-28 2005-11-17 Genorx, Inc. Nanoscale biosensor device, system and technique
WO2006067276A1 (en) * 2004-12-23 2006-06-29 Nanolab Systems Oy Utilization of nanowires in chemical and biological analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SEBASTIAN M LUBER ET AL: "Nanometre spaced electrodes on a cleaved AlGaAs surface; Nanometre spaced electrodes on a cleaved AlGaAs surface", NANOTECHNOLOGY, IOP, BRISTOL, GB, vol. 16, no. 8, 1 August 2005 (2005-08-01), pages 1182 - 1185, XP020091195, ISSN: 0957-4484 *
TREMBLAY F ET AL: "DESIGN NOTE; Hopping in a low-mobility GaAs-AlGaAs heterojunction in the limit of low electronic concentrations", JOURNAL OF PHYSICS: CONDENSED MATTER, INSTITUTE OF PHYSICS PUBLISHING, BRISTOL, GB, vol. 2, no. 35, 3 September 1990 (1990-09-03), pages 7367 - 7371, XP020058664, ISSN: 0953-8984 *

Also Published As

Publication number Publication date
WO2008139421A2 (en) 2008-11-20
ITTO20070341A1 (en) 2008-11-16

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