WO2008121342A3 - Emulsions and techniques for formation - Google Patents

Emulsions and techniques for formation Download PDF

Info

Publication number
WO2008121342A3
WO2008121342A3 PCT/US2008/004097 US2008004097W WO2008121342A3 WO 2008121342 A3 WO2008121342 A3 WO 2008121342A3 US 2008004097 W US2008004097 W US 2008004097W WO 2008121342 A3 WO2008121342 A3 WO 2008121342A3
Authority
WO
WIPO (PCT)
Prior art keywords
emulsions
droplets
techniques
contain
formation
Prior art date
Application number
PCT/US2008/004097
Other languages
French (fr)
Other versions
WO2008121342A2 (en
Inventor
Liang-Yin Chu
Ho Cheung Shum
Alberto Fernandez-Nieves
Andrew S. Utada
Enric Santanach Carreras
David A. Weitz
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Priority to EP08742358A priority Critical patent/EP2136786B8/en
Priority to CN2008800178454A priority patent/CN102014871A/en
Publication of WO2008121342A2 publication Critical patent/WO2008121342A2/en
Publication of WO2008121342A3 publication Critical patent/WO2008121342A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/113—Multiple emulsions, e.g. oil-in-water-in-oil
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/30—Injector mixers
    • B01F25/31—Injector mixers in conduits or tubes through which the main component flows
    • B01F25/313—Injector mixers in conduits or tubes through which the main component flows wherein additional components are introduced in the centre of the conduit
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
    • B01F33/3011—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/80—Mixing plants; Combinations of mixers
    • B01F33/81—Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/80—Mixing plants; Combinations of mixers
    • B01F33/81—Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles
    • B01F33/811—Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles in two or more consecutive, i.e. successive, mixing receptacles or being consecutively arranged
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/06—Making microcapsules or microballoons by phase separation
    • B01J13/14—Polymerisation; cross-linking
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K23/00—Use of substances as emulsifying, wetting, dispersing, or foam-producing agents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/5089—Processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F2025/91—Direction of flow or arrangement of feed and discharge openings
    • B01F2025/915—Reverse flow, i.e. flow changing substantially 180° in direction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991—Coated
    • Y10T428/2998—Coated including synthetic resin or polymer

Abstract

The present invention generally relates to emulsions such as multiple emulsions, and to methods and apparatuses for making emulsions, and techniques for using the same. A multiple emulsion generally describes larger droplets that contain one or more smaller droplets therein which, in some cases, can contain even smaller droplets therein, etc. Emulsions, including multiple emulsions can be formed in certain embodiments with generally precise repeatability, and can be tailored to include any number of inner droplets, in any desired nesting arrangement, within a single outer droplet. In addition, in some aspects of the invention, one or more droplets may be controllably released from a surrounding droplet.
PCT/US2008/004097 2007-03-28 2008-03-28 Emulsions and techniques for formation WO2008121342A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP08742358A EP2136786B8 (en) 2007-03-28 2008-03-28 Apparatus for forming droplets
CN2008800178454A CN102014871A (en) 2007-03-28 2008-03-28 Emulsions and techniques for formation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92057407P 2007-03-28 2007-03-28
US60/920,574 2007-03-28

Publications (2)

Publication Number Publication Date
WO2008121342A2 WO2008121342A2 (en) 2008-10-09
WO2008121342A3 true WO2008121342A3 (en) 2009-10-08

Family

ID=39720479

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/004097 WO2008121342A2 (en) 2007-03-28 2008-03-28 Emulsions and techniques for formation

Country Status (4)

Country Link
US (1) US7776927B2 (en)
EP (1) EP2136786B8 (en)
CN (1) CN102014871A (en)
WO (1) WO2008121342A2 (en)

Families Citing this family (247)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507921A (en) * 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
US20060078893A1 (en) * 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
EP2266687A3 (en) * 2003-04-10 2011-06-29 The President and Fellows of Harvard College Formation and control of fluidic species
JP4630870B2 (en) 2003-08-27 2011-02-09 プレジデント アンド フェロウズ オブ ハーバード カレッジ Electronic control of fluid species
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
US9039273B2 (en) 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
WO2007081386A2 (en) * 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use
US20070195127A1 (en) * 2006-01-27 2007-08-23 President And Fellows Of Harvard College Fluidic droplet coalescence
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US20080014589A1 (en) 2006-05-11 2008-01-17 Link Darren R Microfluidic devices and methods of use thereof
GB0614835D0 (en) 2006-07-26 2006-09-06 Isis Innovation Formation of bilayers of amphipathic molecules
EP2077912B1 (en) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US7776927B2 (en) 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation
US8592221B2 (en) * 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20090068170A1 (en) * 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
WO2009020633A2 (en) * 2007-08-07 2009-02-12 President And Fellows Of Harvard College Metal oxide coating on surfaces
WO2009048532A2 (en) * 2007-10-05 2009-04-16 President And Fellows Of Harvard College Formation of particles for ultrasound application, drug release, and other uses, and microfluidic methods of preparation
JP5738597B2 (en) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ Systems and methods for nucleic acid sequencing
US8802027B2 (en) * 2008-03-28 2014-08-12 President And Fellows Of Harvard College Surfaces, including microfluidic channels, with controlled wetting properties
WO2009149257A1 (en) * 2008-06-04 2009-12-10 The University Of Chicago The chemistrode: a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution
FR2934050B1 (en) 2008-07-15 2016-01-29 Univ Paris Curie METHOD AND DEVICE FOR READING EMULSION
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
WO2010033200A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
WO2011120006A1 (en) 2010-03-25 2011-09-29 Auantalife, Inc. A Delaware Corporation Detection system for droplet-based assays
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9598725B2 (en) * 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US20110045095A1 (en) * 2008-10-08 2011-02-24 The Regents Of The University Of California Polymer-phospholipid shelled microbubbles
EP2373812B1 (en) 2008-12-19 2016-11-09 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
US9307787B2 (en) * 2008-12-19 2016-04-12 U.S. Smokeless Tobacco Company Llc Tobacco granules and method of producing tobacco granules
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
KR101793744B1 (en) 2009-03-13 2017-11-03 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Scale-up of flow-focusing microfluidic devices
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US20110129903A1 (en) * 2009-04-02 2011-06-02 Manuel Marquez Method of making an artificial micro-gland using taxis
WO2011017524A1 (en) * 2009-08-05 2011-02-10 The Trustees Of The University Of Pennsylvania Microfluidic-based generation of functionalized microbubbles for ultrasound imaging and therapy
ES2533498T3 (en) * 2009-08-28 2015-04-10 Georgia Tech Research Corporation Method and electro-fluidic device to produce emulsions and suspension of particles
KR20120089662A (en) * 2009-09-02 2012-08-13 바스프 에스이 Multiple emulsions created using junctions
US20120211084A1 (en) * 2009-09-02 2012-08-23 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
JP6155418B2 (en) 2009-09-02 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド System for mixing fluids by combining multiple emulsions
EP2486409A1 (en) 2009-10-09 2012-08-15 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
JP5791621B2 (en) 2009-10-27 2015-10-07 プレジデント アンド フェローズ オブ ハーバード カレッジ Droplet generation technology
WO2011056390A2 (en) * 2009-11-03 2011-05-12 Manuel Marquez Method of making an artificial micro-gland that is anisotropic
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US9151646B2 (en) 2011-12-21 2015-10-06 Deka Products Limited Partnership System, method, and apparatus for monitoring, regulating, or controlling fluid flow
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
AR080405A1 (en) * 2010-03-17 2012-04-04 Basf Se EMULSIFICATION TO FOUND
CA2767114A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Droplet transport system for detection
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
US8531517B2 (en) * 2010-07-15 2013-09-10 Kai Tao IV monitoring by video and image processing
CN103153466B (en) 2010-07-22 2016-04-13 基因细胞生物系统有限公司 Composite fluid pond
WO2012021838A1 (en) * 2010-08-13 2012-02-16 University Of Connecticut Co-flow microfluidic device for polymersome formation
GB2482911A (en) * 2010-08-20 2012-02-22 Sphere Fluidics Ltd Microdroplet emulsion system
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
CA3024250C (en) * 2010-11-01 2022-01-04 Bio-Rad Laboratories, Inc. System for forming emulsions
WO2012087350A2 (en) 2010-12-21 2012-06-28 President And Fellows Of Harvard College Spray drying techniques
US10258941B2 (en) 2010-12-23 2019-04-16 Evonik Corporation Apparatus and methods for preparing an emulsion
FR2969907B1 (en) * 2010-12-31 2014-03-07 Capsum SERIES OF CAPSULES COMPRISING AT LEAST ONE INTERNAL PHASE DROP IN AN INTERMEDIATE PHASE DROP AND METHOD OF MANUFACTURING THE SAME
WO2012109138A1 (en) 2011-02-07 2012-08-16 President And Fellows Of Harvard College Systems and methods for splitting droplets
WO2012109374A2 (en) 2011-02-08 2012-08-16 Lawrence Livermore National Security, Llc Tethered catalysts for the hydration of carbon dioxide
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
US8834605B2 (en) 2011-02-18 2014-09-16 Lawrence Livermore National Security, Llc. Separation of a target substance from a fluid or mixture using encapsulated sorbents
CN103534360A (en) 2011-03-18 2014-01-22 伯乐生命医学产品有限公司 Multiplexed digital assays with combinatorial use of signals
CN102205227B (en) * 2011-03-20 2013-07-10 四川大学 Micro-fluidic method for preparing monodisperse multicomponent multiple emulsion, and apparatus thereof
JP6472998B2 (en) 2011-03-30 2019-02-20 バイオ−ラッド ラボラトリーズ インコーポレイテッド Multiple volume injection into or from a droplet
EP3789498A1 (en) 2011-04-25 2021-03-10 Bio-rad Laboratories, Inc. Methods for nucleic acid analysis
WO2012162296A2 (en) 2011-05-23 2012-11-29 President And Fellows Of Harvard College Control of emulsions, including multiple emulsions
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
CN103764265A (en) * 2011-07-06 2014-04-30 哈佛学院院长等 Multiple emulsions and techniques for the formation of multiple emulsions
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
CN102389730A (en) * 2011-08-23 2012-03-28 东南大学 Double-emulsion preparation chip
CN103764272A (en) 2011-08-30 2014-04-30 哈佛学院院长等 Systems and methods for shell encapsulation
WO2013095737A2 (en) 2011-09-28 2013-06-27 President And Fellows Of Harvard College Systems and methods for droplet production and/or fluidic manipulation
GB201119032D0 (en) 2011-11-03 2011-12-14 Isis Innovation Multisomes: encapsulated droplet networks
US9746093B2 (en) 2011-12-21 2017-08-29 Deka Products Limited Partnership Flow meter and related system and apparatus
US10488848B2 (en) 2011-12-21 2019-11-26 Deka Products Limited Partnership System, method, and apparatus for monitoring, regulating, or controlling fluid flow
US9724467B2 (en) 2011-12-21 2017-08-08 Deka Products Limited Partnership Flow meter
US9435455B2 (en) 2011-12-21 2016-09-06 Deka Products Limited Partnership System, method, and apparatus for monitoring, regulating, or controlling fluid flow
US9746094B2 (en) 2011-12-21 2017-08-29 Deka Products Limited Partnership Flow meter having a background pattern with first and second portions
US10228683B2 (en) 2011-12-21 2019-03-12 Deka Products Limited Partnership System, method, and apparatus for monitoring, regulating, or controlling fluid flow
US9372486B2 (en) 2011-12-21 2016-06-21 Deka Products Limited Partnership System, method, and apparatus for monitoring, regulating, or controlling fluid flow
EP2807255B1 (en) 2012-01-25 2017-08-02 Gencell Biosystems Limited Biomolecule isolation
BR112014019323A8 (en) * 2012-02-08 2017-07-11 Harvard College DROPLET FORMATION USING FLUID DECOMPOSITION
US10080997B2 (en) * 2012-03-16 2018-09-25 Versitech Limited System and method for generation of emulsions with low interfacial tension and measuring frequency vibrations in the system
US9782733B2 (en) * 2012-03-22 2017-10-10 Universiteit Twente Apparatus and method for mass producing a monodisperse microbubble agent
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US9597648B2 (en) 2012-10-17 2017-03-21 The Procter & Gamble Company Non-spherical droplet
GB201219201D0 (en) 2012-10-25 2012-12-12 Isis Innovation Hydrogel network
GB201219196D0 (en) 2012-10-25 2012-12-12 Isis Innovation Droplet assembly method
CN104812492A (en) 2012-11-27 2015-07-29 基因细胞生物系统有限公司 Handling liquid samples
WO2014087175A2 (en) * 2012-12-07 2014-06-12 Isis Innovation Limited Droplet assembly by 3d printing
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9759343B2 (en) 2012-12-21 2017-09-12 Deka Products Limited Partnership Flow meter using a dynamic background image
EP3862435A1 (en) * 2013-02-08 2021-08-11 10X Genomics, Inc. Polynucleotide barcode generation
US9279321B2 (en) 2013-03-06 2016-03-08 Lawrence Livermore National Security, Llc Encapsulated microsensors for reservoir interrogation
US20150375915A1 (en) * 2013-04-18 2015-12-31 Empire Technology Development Llc Thermostatic packaging materials
CN103240043B (en) * 2013-05-09 2014-08-13 四川大学 Preparation method of monodisperse multicomponent multiple emulsion
US20140335172A1 (en) * 2013-05-10 2014-11-13 The University Of Hong Kong Ophthalmological rinsing agent and methods therefof
US9867763B2 (en) * 2013-05-10 2018-01-16 Noxell Corporation Modular emulsion-based product differentiation
WO2014194272A2 (en) * 2013-05-31 2014-12-04 University Of Washington Through Its Center For Commercialization Droplet-mass spectrometer interface
US9534964B2 (en) 2013-06-26 2017-01-03 Temptime Corporation Three-phase emulsions used in a temperature condition indicator
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9233859B2 (en) 2013-09-30 2016-01-12 Uchicago Argonne, Llc. Microfluidic process monitor for industrial solvent extraction system
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
FR3012050B1 (en) * 2013-10-17 2016-01-01 Capsum METHOD OF FORMING A DISPERSION COMPRISING DROPS, AND APPARATUS THEREFOR
US20150174549A1 (en) * 2013-10-25 2015-06-25 The Brigham And Women's Hospital Corporation High-throughput synthesis of nanoparticles
USD749206S1 (en) 2013-11-06 2016-02-09 Deka Products Limited Partnership Apparatus to control fluid flow through a tube
USD751689S1 (en) 2013-11-06 2016-03-15 Deka Products Limited Partnership Apparatus to control fluid flow through a tube
USD745661S1 (en) 2013-11-06 2015-12-15 Deka Products Limited Partnership Apparatus to control fluid flow through a tube
USD751690S1 (en) 2013-11-06 2016-03-15 Deka Products Limited Partnership Apparatus to control fluid flow through a tube
USD752209S1 (en) 2013-11-06 2016-03-22 Deka Products Limited Partnership Apparatus to control fluid flow through a tube
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
GB201321433D0 (en) * 2013-12-04 2014-01-15 Isis Innovation Controling fluid micro compartments
WO2015085899A1 (en) * 2013-12-09 2015-06-18 The University Of Hong Kong Stabilized all-aqueous emulsions and methods of making and using thereof
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP3090063B1 (en) 2013-12-31 2019-11-06 Bio-Rad Laboratories, Inc. Method for detection of latent retrovirus
JP2017505920A (en) * 2014-01-14 2017-02-23 センター ナショナル デ ラ リシェルシェ サイエンティフィック（シーエヌアールエス） Microfluidic device for analysis of flowing contaminants
FR3016441B1 (en) * 2014-01-14 2017-06-16 Centre Nat De La Rech Scient (Cnrs) MICROFLUIDIC DEVICE FOR THE ANALYSIS OF FLOW POLLUTANTS
WO2015120398A1 (en) 2014-02-10 2015-08-13 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
WO2015160919A1 (en) 2014-04-16 2015-10-22 President And Fellows Of Harvard College Systems and methods for producing droplet emulsions with relatively thin shells
US10232373B2 (en) 2014-06-16 2019-03-19 Bio-Rad Laboratories, Inc. Size alternating injection into drops to facilitate sorting
KR20170023979A (en) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. Processes and systems for nucleic acid sequence assembly
CN106795553B (en) 2014-06-26 2021-06-04 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
US9981237B2 (en) * 2014-08-19 2018-05-29 New York University Higher order multiple emulsions
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
JP2017537776A (en) * 2014-11-24 2017-12-21 ザ プロクター アンド ギャンブル カンパニー System for encapsulation of active substances in droplets and other compartments
EP3224419A4 (en) * 2014-11-24 2018-06-27 President and Fellows of Harvard College Multiple emulsions comprising rigidified portions
CN105727857A (en) * 2014-12-10 2016-07-06 黑龙江鑫达企业集团有限公司 Microfluidic apparatus produced by 3D printing
EP3244992B1 (en) 2015-01-12 2023-03-08 10X Genomics, Inc. Processes for barcoding nucleic acids
JP2018508852A (en) 2015-01-13 2018-03-29 １０エックス ゲノミクス，インコーポレイテッド System and method for visualizing structural variation and phase information
MX2017010142A (en) 2015-02-09 2017-12-11 10X Genomics Inc Systems and methods for determining structural variation and phasing using variant call data.
US9714897B2 (en) 2015-02-09 2017-07-25 Slingshot Biosciences, Inc. Hydrogel particles with tunable optical properties and methods for using the same
BR112017018054A2 (en) 2015-02-24 2018-07-24 10X Genomics Inc Methods for Covering Targeted Nucleic Acid Sequences
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
EP3341508A4 (en) 2015-08-25 2019-05-15 Bio-Rad Laboratories, Inc. Digital immunoassay
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US10807117B2 (en) * 2015-10-05 2020-10-20 Tokyo Electron Limited Dispense nozzle with a dynamic liquid plug
WO2017066231A1 (en) * 2015-10-13 2017-04-20 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
US11542531B2 (en) * 2016-01-21 2023-01-03 Lawrence Livermore National Security, Llc Biocatalytic microcapsules for catalyzing gas conversion
USD905848S1 (en) 2016-01-28 2020-12-22 Deka Products Limited Partnership Apparatus to control fluid flow through a tube
CN108697845B (en) 2016-01-28 2021-09-17 德卡产品有限公司 Apparatus for monitoring, regulating or controlling fluid flow
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
CN105641743B (en) * 2016-03-16 2019-05-17 宁波瑞柏思生物材料科技有限公司 A kind of micro fluidic device and the method for preparing microgel using the device
CN105833813B (en) * 2016-03-24 2018-04-10 北京科技大学 Room temperature drop microreactor and the method for preparing zirconium dioxide gel micro-ball
WO2017165620A1 (en) * 2016-03-24 2017-09-28 Bio-Rad Laboratories, Inc. Use of gel beads to control droplet dispersion
CN107349880B (en) * 2016-05-09 2020-10-09 香港大学深圳医院 Method for preparing core-shell type microspheres and coaxial microfluid device applied to method
CN105771825A (en) * 2016-05-11 2016-07-20 中国工程物理研究院激光聚变研究中心 Emulsion particle generator for continuous production
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
USD854145S1 (en) 2016-05-25 2019-07-16 Deka Products Limited Partnership Apparatus to control fluid flow through a tube
CN106047485B (en) * 2016-07-04 2020-04-21 云南芯韵科技开发有限公司 Water capsule for cigarettes and preparation method thereof
CN106140037B (en) * 2016-07-06 2019-05-31 东南大学 A kind of preparation method and applications of the microcapsules of wellability guidance
US10406336B2 (en) 2016-08-03 2019-09-10 Neil S. Davey Adjustable rate drug delivery implantable device
US10654040B2 (en) 2016-08-18 2020-05-19 Northeastern University Platform for liquid droplet formation and isolation
CN106540638A (en) * 2016-11-25 2017-03-29 复旦大学 It is a kind of based on drop it is micro-/the milli fluidics method for preparing capsule
CN106492716B (en) * 2016-12-20 2024-01-30 中国工程物理研究院激光聚变研究中心 Integrated double-emulsion particle generating device and processing method thereof
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3554990A4 (en) * 2017-02-15 2019-12-25 Hewlett-Packard Development Company, L.P. Microfluidic valve
EP3625353B1 (en) 2017-05-18 2022-11-30 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Microfluidic channel networks for partitioning
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
CN112236218B (en) * 2018-04-02 2022-04-26 滴管公司 System and method for continuous flow emulsion processing
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
JP7065357B2 (en) * 2018-07-10 2022-05-12 パナソニックＩｐマネジメント株式会社 Mist generator
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN109091705B (en) * 2018-10-23 2019-08-06 吕洋 A kind of three-dimensional porous rack and its preparation method and application
US10731012B2 (en) * 2018-11-06 2020-08-04 President And Fellows Of Harvard College Anti-clogging microfluidic multichannel device
GB201818936D0 (en) 2018-11-21 2019-01-02 Univ Loughborough Microfluid device
CN109856300A (en) * 2018-11-22 2019-06-07 天津大学 A kind of preparation method of silica inverse opal hydrogel photonic crystal microballoon
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
US20220233413A1 (en) * 2019-05-07 2022-07-28 The Regents Of The University Of California Monodisperse emulsions templated by three-dimensional structured microparticles and methods of making the same
USD964563S1 (en) 2019-07-26 2022-09-20 Deka Products Limited Partnership Medical flow clamp
US11839741B2 (en) 2019-07-26 2023-12-12 Deka Products Limited Partneship Apparatus for monitoring, regulating, or controlling fluid flow
CN112439540B (en) * 2019-08-30 2022-08-30 中国石油天然气股份有限公司 Light and heavy mineral separation device and method
CN115004009A (en) 2020-01-24 2022-09-02 弹弓生物科学公司 Compositions and methods for cell-like calibration particles
US20210291129A1 (en) * 2020-03-23 2021-09-23 Mark A. Gray Capillary tube droplet generation systems and methods
JP2023520063A (en) * 2020-04-01 2023-05-15 メルク パテント ゲーエムベーハー Emulsifying equipment
EP4147049A1 (en) 2020-05-04 2023-03-15 Slingshot Biosciences, Inc. Compositions and methods for passive optical barcoding for multiplexed assays
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN112452251B (en) * 2020-10-27 2021-12-14 山东大学 Crescent-shaped and deformed ceramic microparticle, preparation method, application and preparation device thereof
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN113019348B (en) * 2021-03-15 2022-04-26 厦门大学 Method for preparing chromatographic packing based on capillary droplet microfluidics
CA3235582A1 (en) 2021-10-29 2023-05-04 Anh Tuan Nguyen Hydrogel particles as feeder cells and as synthetic antigen presenting cells
WO2023117364A1 (en) 2021-12-01 2023-06-29 Vilnius University A microcapsule and methods of making and using same
WO2023099661A1 (en) 2021-12-01 2023-06-08 Vilnius University Microcapsules comprising biological samples, and methods for use of same
GB2616677A (en) * 2022-03-18 2023-09-20 Sphere Fluidics Ltd Droplet formation system and method
WO2023215886A1 (en) 2022-05-05 2023-11-09 Slingshot Biosciences, Inc. Engineered particles as red blood cell mimics and compositions containing same for hematology
CN115445461A (en) * 2022-08-17 2022-12-09 广东省科学院生物与医学工程研究所 Double-layer micro-droplet generation device and double-layer micro-droplet generation method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH563807A5 (en) * 1973-02-14 1975-07-15 Battelle Memorial Institute Fine granules and microcapsules mfrd. from liquid droplets - partic. of high viscosity requiring forced sepn. of droplets
WO2006096571A2 (en) * 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20060263888A1 (en) * 2000-06-02 2006-11-23 Honeywell International Inc. Differential white blood count on a disposable card
GB2433448A (en) * 2005-12-20 2007-06-27 Q Chip Ltd Device and method for the control of chemical processes

Family Cites Families (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3980541A (en) 1967-06-05 1976-09-14 Aine Harry E Electrode structures for electric treatment of fluids and filters using same
CH564966A5 (en) 1974-02-25 1975-08-15 Sauter Fr Ag Fabrik Elektrisch
US4279345A (en) 1979-08-03 1981-07-21 Allred John C High speed particle sorter using a field emission electrode
JPS6057907B2 (en) 1981-06-18 1985-12-17 工業技術院長 Liquid mixing and atomization method
US4865444A (en) 1984-04-05 1989-09-12 Mobil Oil Corporation Apparatus and method for determining luminosity of hydrocarbon fuels
US4916070A (en) 1986-04-14 1990-04-10 The General Hospital Corporation Fibrin-specific antibodies and method of screening for the antibodies
US5204112A (en) 1986-06-16 1993-04-20 The Liposome Company, Inc. Induction of asymmetry in vesicles
US4931225A (en) 1987-12-30 1990-06-05 Union Carbide Industrial Gases Technology Corporation Method and apparatus for dispersing a gas into a liquid
US5093602A (en) 1989-11-17 1992-03-03 Charged Injection Corporation Methods and apparatus for dispersing a fluent material utilizing an electron beam
US6149789A (en) 1990-10-31 2000-11-21 Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Process for manipulating microscopic, dielectric particles and a device therefor
SE500071C2 (en) 1992-06-25 1994-04-11 Vattenfall Utveckling Ab Device for mixing two fluids, in particular liquids of different temperature
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
DE69519197T2 (en) 1994-06-13 2001-05-17 Praxair Technology Inc Atomizer for the combustion of liquid fuel with a small spray angle
US5935331A (en) 1994-09-09 1999-08-10 Matsushita Electric Industrial Co., Ltd. Apparatus and method for forming films
US5762775A (en) 1994-09-21 1998-06-09 Lockheed Martin Energy Systems, Inc. Method for electrically producing dispersions of a nonconductive fluid in a conductive medium
EP0812434B1 (en) 1995-03-01 2013-09-18 President and Fellows of Harvard College Microcontact printing on surfaces and derivative articles
US5681600A (en) 1995-12-18 1997-10-28 Abbott Laboratories Stabilization of liquid nutritional products and method of making
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US6189803B1 (en) 1996-05-13 2001-02-20 University Of Seville Fuel injection nozzle and method of use
US6248378B1 (en) 1998-12-16 2001-06-19 Universidad De Sevilla Enhanced food products
US6196525B1 (en) 1996-05-13 2001-03-06 Universidad De Sevilla Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber
US6299145B1 (en) 1996-05-13 2001-10-09 Universidad De Sevilla Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber
US6197835B1 (en) 1996-05-13 2001-03-06 Universidad De Sevilla Device and method for creating spherical particles of uniform size
US6386463B1 (en) 1996-05-13 2002-05-14 Universidad De Sevilla Fuel injection nozzle and method of use
ES2140998B1 (en) 1996-05-13 2000-10-16 Univ Sevilla LIQUID ATOMIZATION PROCEDURE.
US6405936B1 (en) 1996-05-13 2002-06-18 Universidad De Sevilla Stabilized capillary microjet and devices and methods for producing same
US6187214B1 (en) 1996-05-13 2001-02-13 Universidad De Seville Method and device for production of components for microfabrication
NZ333346A (en) 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
DE69709377T2 (en) 1996-09-04 2002-08-14 Scandinavian Micro Biodevices MICROFLOWING SYSTEM FOR PARTICLE ANALYSIS AND SEPARATION
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6120666A (en) 1996-09-26 2000-09-19 Ut-Battelle, Llc Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same
DK1019496T3 (en) 1997-07-07 2005-01-10 Medical Res Council In vitro sorting method
US5980936A (en) 1997-08-07 1999-11-09 Alliance Pharmaceutical Corp. Multiple emulsions comprising a hydrophobic continuous phase
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US6614598B1 (en) 1998-11-12 2003-09-02 Institute Of Technology, California Microlensing particles and applications
US6450189B1 (en) 1998-11-13 2002-09-17 Universidad De Sevilla Method and device for production of components for microfabrication
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
EP1179087B1 (en) 1999-05-17 2019-03-27 Caliper Life Sciences, Inc. Focusing of microparticles in microfluidic systems
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
ES2424713T4 (en) 1999-06-11 2014-01-23 Aradigm Corporation Method of producing a spray
US7601270B1 (en) 1999-06-28 2009-10-13 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US6890487B1 (en) 1999-09-30 2005-05-10 Science & Technology Corporation ©UNM Flow cytometry for high throughput screening
DE19961257C2 (en) 1999-12-18 2002-12-19 Inst Mikrotechnik Mainz Gmbh micromixer
AU2001232805A1 (en) 2000-01-12 2001-07-24 Ut-Battelle, Llc A microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
CN1429181A (en) 2000-03-10 2003-07-09 流体聚焦公司 Methods for producing optical fiber by focusing high viscosity liquid
US7485454B1 (en) 2000-03-10 2009-02-03 Bioprocessors Corp. Microreactor
DE10015109A1 (en) 2000-03-28 2001-10-04 Peter Walzel Processes and devices for producing drops of equal size
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US6686184B1 (en) 2000-05-25 2004-02-03 President And Fellows Of Harvard College Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks
US6777450B1 (en) 2000-05-26 2004-08-17 Color Access, Inc. Water-thin emulsions with low emulsifier levels
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6301055B1 (en) 2000-08-16 2001-10-09 California Institute Of Technology Solid immersion lens structures and methods for producing solid immersion lens structures
DE10041823C2 (en) 2000-08-25 2002-12-19 Inst Mikrotechnik Mainz Gmbh Method and static micromixer for mixing at least two fluids
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
US6508988B1 (en) 2000-10-03 2003-01-21 California Institute Of Technology Combinatorial synthesis system
US6778724B2 (en) 2000-11-28 2004-08-17 The Regents Of The University Of California Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices
EP1385488A2 (en) 2000-12-07 2004-02-04 President And Fellows Of Harvard College Methods and compositions for encapsulating active agents
US20040096515A1 (en) 2001-12-07 2004-05-20 Bausch Andreas R. Methods and compositions for encapsulating active agents
EP1741482B1 (en) 2001-02-23 2008-10-15 Japan Science and Technology Agency Process and apparatus for producing microcapsules
JP3746766B2 (en) 2001-02-23 2006-02-15 独立行政法人科学技術振興機構 Emulsion production method and apparatus
US6752922B2 (en) 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
EP1399580B1 (en) 2001-05-26 2008-10-08 One Cell Systems, Inc. Secretion of proteins by encapsulated cells
GB0114854D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
US20030015425A1 (en) 2001-06-20 2003-01-23 Coventor Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
AU2002319668A1 (en) 2001-07-27 2003-02-17 President And Fellows Of Harvard College Laminar mixing apparatus and methods
US6976590B2 (en) 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
JP4855680B2 (en) 2002-05-09 2012-01-18 ザ・ユニバーシティ・オブ・シカゴ Apparatus and method for transport and reaction by pressure driven plug
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
GB2395196B (en) 2002-11-14 2006-12-27 Univ Cardiff Microfluidic device and methods for construction and application
WO2004071638A2 (en) 2003-02-11 2004-08-26 Regents Of The University Of California, The Microfluidic devices and method for controlled viscous shearing and formation of amphiphilic vesicles
US7045040B2 (en) 2003-03-20 2006-05-16 Asm Nutool, Inc. Process and system for eliminating gas bubbles during electrochemical processing
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
EP2266687A3 (en) 2003-04-10 2011-06-29 The President and Fellows of Harvard College Formation and control of fluidic species
US7115230B2 (en) 2003-06-26 2006-10-03 Intel Corporation Hydrodynamic focusing devices
GB0315438D0 (en) 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
US20050032238A1 (en) 2003-08-07 2005-02-10 Nanostream, Inc. Vented microfluidic separation devices and methods
JP4630870B2 (en) 2003-08-27 2011-02-09 プレジデント アンド フェロウズ オブ ハーバード カレッジ Electronic control of fluid species
CA2536360C (en) 2003-08-28 2013-08-06 Celula, Inc. Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev Compositions and methods for in vitro sorting of molecular and cellular libraries
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
WO2005103106A1 (en) 2004-04-23 2005-11-03 Eugenia Kumacheva Method of producing polymeric particles with selected size, shape, morphology and composition
WO2006002641A1 (en) 2004-07-02 2006-01-12 Versamatrix A/S Spherical radiofrequency-encoded beads
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7759111B2 (en) 2004-08-27 2010-07-20 The Regents Of The University Of California Cell encapsulation microfluidic device
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006078841A1 (en) 2005-01-21 2006-07-27 President And Fellows Of Harvard College Systems and methods for forming fluidic droplets encapsulated in particles such as colloidal particles
US20070054119A1 (en) 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
US8734003B2 (en) 2005-09-15 2014-05-27 Alcatel Lucent Micro-chemical mixing
WO2007081386A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use
US20070195127A1 (en) 2006-01-27 2007-08-23 President And Fellows Of Harvard College Fluidic droplet coalescence
US20080014589A1 (en) 2006-05-11 2008-01-17 Link Darren R Microfluidic devices and methods of use thereof
US7776927B2 (en) 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH563807A5 (en) * 1973-02-14 1975-07-15 Battelle Memorial Institute Fine granules and microcapsules mfrd. from liquid droplets - partic. of high viscosity requiring forced sepn. of droplets
US20060263888A1 (en) * 2000-06-02 2006-11-23 Honeywell International Inc. Differential white blood count on a disposable card
WO2006096571A2 (en) * 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
GB2433448A (en) * 2005-12-20 2007-06-27 Q Chip Ltd Device and method for the control of chemical processes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EXPERIMENTAL SOFT CONDENSED MATTER GROUP, HARVARD UNIVERSITY: "Cool Picture of the Moment", INTERNET ARTICLE, 16 January 2007 (2007-01-16), XP002514708, Retrieved from the Internet <URL:http://www.seas.harvard.edu/projects/weitzlab/coolpic16012007.html> [retrieved on 20090211] *
OKUSHIMA SHINGO ET AL: "Controlled production of monodisperse double emulsions by two-step droplet breakup in microfluidic devices", LANGMUIR, ACS, WASHINGTON, DC, US, vol. 20, no. 23, 9 November 2004 (2004-11-09), pages 9905 - 9908, XP002376054, ISSN: 0743-7463 *

Also Published As

Publication number Publication date
US20090012187A1 (en) 2009-01-08
CN102014871A (en) 2011-04-13
EP2136786B8 (en) 2012-11-14
EP2136786A2 (en) 2009-12-30
EP2136786B1 (en) 2012-10-10
US7776927B2 (en) 2010-08-17
WO2008121342A2 (en) 2008-10-09

Similar Documents

Publication Publication Date Title
WO2008121342A3 (en) Emulsions and techniques for formation
WO2009048532A3 (en) Formation of particles for ultrasound application, drug release, and other uses, and microfluidic methods of preparation
WO2006096571A3 (en) Method and apparatus for forming multiple emulsions
WO2011079176A3 (en) Microfluidic systems and methods for reducing the exchange of molecules between droplets
BR112013005118A2 (en) drink cartridge
MX292905B (en) Emulsions and microcapsules with substances having low interfacial tension, methods of making and using thereof.
WO2012116246A3 (en) Pesticidal compostions and processes related thereto
CL2013001836A1 (en) Pesticide mixture comprising at least one pyrazole compound; pesticidal or parasiticidal composition; method to control arachnoid insects or nematodes, and harmful phytopathogenic fungi.
EP2594604A3 (en) Azeotrope-like compositions comprising 1-chloro-3,3,3-trifluoropropene
EP2447274A3 (en) Oligomeric compounds and methods
WO2009125401A3 (en) Compositions and methods for enhancing oil content in plants
WO2008014291A3 (en) Compounds and compositions as hedgehog signaling pathway modulators
EP2287349A4 (en) Heat-resistant austenitic alloy, heat-resistant pressure-resistant member comprising the alloy, and process for producing the same
HK1141713A1 (en) Oil-in-water emulsion composition and method for producing the same
WO2013162716A3 (en) Pesticidal compositions and processes related thereto
WO2012162296A3 (en) Control of emulsions, including multiple emulsions
WO2008003907A3 (en) Method and apparatus for injecting a jet of fluid with a variable direction and/or opening
EP2060187A4 (en) Edible oil or fat, process for producing the same, and chocolate containing oil or fat composition
CL2014000366A1 (en) Pollution-resistant composition comprising an elastomer and a component selected from acylated lanolin, alkoxylated lanolin or lanolin oil; method; use of the composition; and substrate.
AP2011005592A0 (en) Microencapsulate and process for the manufacture thereof.
WO2008110745A3 (en) Depilatory composition
WO2008003677A3 (en) Anti-dandruff hair conditioning compositions
WO2007139872A3 (en) Spinosyn fumigants
WO2008112659A3 (en) Regulation of osteopontin
BRPI0812871A2 (en) &#34;CONTAINER, AND METHOD FOR FORMING THE SAME&#34;.

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200880017845.4

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08742358

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008742358

Country of ref document: EP