WO2008074023A3 - Systems and methods for multiplex analysis of pcr in real time - Google Patents

Systems and methods for multiplex analysis of pcr in real time Download PDF

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Publication number
WO2008074023A3
WO2008074023A3 PCT/US2007/087492 US2007087492W WO2008074023A3 WO 2008074023 A3 WO2008074023 A3 WO 2008074023A3 US 2007087492 W US2007087492 W US 2007087492W WO 2008074023 A3 WO2008074023 A3 WO 2008074023A3
Authority
WO
WIPO (PCT)
Prior art keywords
systems
pcr
methods
real time
multiplex analysis
Prior art date
Application number
PCT/US2007/087492
Other languages
French (fr)
Other versions
WO2008074023A2 (en
Inventor
Douglas F Whitman
Charles J Collins
Original Assignee
Luminex Corp
Douglas F Whitman
Charles J Collins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Luminex Corp, Douglas F Whitman, Charles J Collins filed Critical Luminex Corp
Priority to BRPI0721095A priority Critical patent/BRPI0721095B1/en
Priority to JP2009541602A priority patent/JP5479910B2/en
Priority to EP16183121.9A priority patent/EP3118333B1/en
Priority to KR1020097013763A priority patent/KR101433208B1/en
Priority to CN2007800512972A priority patent/CN101657548B/en
Priority to AU2007333040A priority patent/AU2007333040B2/en
Priority to CA2672034A priority patent/CA2672034C/en
Priority to EP07869243.1A priority patent/EP2099930B1/en
Publication of WO2008074023A2 publication Critical patent/WO2008074023A2/en
Publication of WO2008074023A3 publication Critical patent/WO2008074023A3/en
Priority to HK10101623.1A priority patent/HK1134525A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143—Magnetism, e.g. magnetic label

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The present invention provides methods and systems for real-time measurements of PCR with multiplexing capability. Certain embodiments relate to methods and systems that use fluorescently encoded superparamagnetic microspheres for the immobilization of amplification products during the PCR process, and an imaging chamber of a measurement device that is also capable of controllable thermal cycling for assisting the PCR process.
PCT/US2007/087492 2006-12-13 2007-12-13 Systems and methods for multiplex analysis of pcr in real time WO2008074023A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
BRPI0721095A BRPI0721095B1 (en) 2006-12-13 2007-12-13 SYSTEMS AND METHODS FOR MULTIPLEX REAL-TIME PCR ANALYSIS
JP2009541602A JP5479910B2 (en) 2006-12-13 2007-12-13 System and method for real-time multiplex analysis of PCR
EP16183121.9A EP3118333B1 (en) 2006-12-13 2007-12-13 Systems and methods for multiplex analysis of pcr in real time
KR1020097013763A KR101433208B1 (en) 2006-12-13 2007-12-13 Systems and methods for multiplex analysis of pcr in real time
CN2007800512972A CN101657548B (en) 2006-12-13 2007-12-13 Systems and methods for multiplex analysis of PCR in real time
AU2007333040A AU2007333040B2 (en) 2006-12-13 2007-12-13 Systems and methods for multiplex analysis of PCR in real time
CA2672034A CA2672034C (en) 2006-12-13 2007-12-13 Systems and methods for multiplex analysis of pcr in real time
EP07869243.1A EP2099930B1 (en) 2006-12-13 2007-12-13 Systems and methods for multiplex analysis of pcr in real time
HK10101623.1A HK1134525A1 (en) 2006-12-13 2010-02-12 Systems and methods for multiplex analysis of pcr in real time pcr

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US86974206P 2006-12-13 2006-12-13
US60/869,742 2006-12-13

Publications (2)

Publication Number Publication Date
WO2008074023A2 WO2008074023A2 (en) 2008-06-19
WO2008074023A3 true WO2008074023A3 (en) 2008-08-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/087492 WO2008074023A2 (en) 2006-12-13 2007-12-13 Systems and methods for multiplex analysis of pcr in real time

Country Status (10)

Country Link
US (7) US7955802B2 (en)
EP (3) EP2857526B1 (en)
JP (1) JP5479910B2 (en)
KR (1) KR101433208B1 (en)
CN (2) CN102851369B (en)
AU (1) AU2007333040B2 (en)
BR (1) BRPI0721095B1 (en)
CA (1) CA2672034C (en)
HK (2) HK1134525A1 (en)
WO (1) WO2008074023A2 (en)

Families Citing this family (137)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101146595B (en) 2005-01-28 2012-07-04 杜克大学 Apparatuses and methods for manipulating droplets on a printed circuit board
US7932037B2 (en) * 2007-12-05 2011-04-26 Perkinelmer Health Sciences, Inc. DNA assays using amplicon probes on encoded particles
AU2006331607B2 (en) * 2005-12-23 2012-11-29 Perkinelmer Health Sciences, Inc. Comparative genomic hybridization on encoded multiplex particles
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US7727723B2 (en) 2006-04-18 2010-06-01 Advanced Liquid Logic, Inc. Droplet-based pyrosequencing
WO2007123908A2 (en) 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Droplet-based multiwell operations
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
WO2009111769A2 (en) 2008-03-07 2009-09-11 Advanced Liquid Logic, Inc. Reagent and sample preparation and loading on a fluidic device
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US7947487B2 (en) 2006-10-05 2011-05-24 Massachusetts Institute Of Technology Multifunctional encoded particles for high-throughput analysis
EP2857526B1 (en) 2006-12-13 2016-08-17 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
BRPI0806831B8 (en) 2007-02-09 2021-07-27 Advanced Liquid Logic Inc droplet actuating methods employing magnetic spheres
US8872527B2 (en) 2007-02-15 2014-10-28 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
EP2126038B1 (en) 2007-03-22 2015-01-07 Advanced Liquid Logic, Inc. Enzymatic assays for a droplet actuator
US8951732B2 (en) 2007-06-22 2015-02-10 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
WO2009029561A2 (en) * 2007-08-24 2009-03-05 Advanced Liquid Logic, Inc. Bead manipulations on a droplet actuator
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
WO2009052095A1 (en) * 2007-10-17 2009-04-23 Advanced Liquid Logic, Inc. Reagent storage and reconstitution for a droplet actuator
WO2009076414A2 (en) * 2007-12-10 2009-06-18 Advanced Liquid Logic, Inc. Droplet actuator configurations and methods
KR20100100974A (en) 2007-12-23 2010-09-15 어드밴스드 리퀴드 로직, 아이엔씨. Droplet actuator configurations and methods of conducting droplet operations
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US20110097763A1 (en) * 2008-05-13 2011-04-28 Advanced Liquid Logic, Inc. Thermal Cycling Method
EP2286228B1 (en) 2008-05-16 2019-04-03 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for manipulating beads
WO2010054385A2 (en) 2008-11-10 2010-05-14 Luminex Corporation Method and system for manufacture and use of macroporous beads in a multiplex assay
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
WO2010077205A1 (en) * 2009-01-05 2010-07-08 Ge Healthcare Bio-Sciences Corp A system and method for simultaneously obtaining a plurality of images in an imaging system
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
EP2221606A3 (en) * 2009-02-11 2012-06-06 Samsung Electronics Co., Ltd. Integrated bio-chip and method of fabricating the integrated bio-chip
WO2010111776A1 (en) * 2009-04-01 2010-10-07 Funglyn Biotech, Inc. Method and system for multiple nucleic acid amplification and quantitation
JP5785942B2 (en) 2009-06-29 2015-09-30 ルミネックス コーポレーション Chimeric primer with hairpin conformation and use thereof
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US20110111403A1 (en) * 2009-10-30 2011-05-12 Life Technologies Corporation Multi-primer assay for mycoplasma detection
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
AU2011221243B2 (en) 2010-02-25 2016-06-02 Advanced Liquid Logic, Inc. Method of making nucleic acid libraries
JP5808349B2 (en) 2010-03-01 2015-11-10 カリス ライフ サイエンシズ スウィッツァーランド ホールディングスゲーエムベーハー Biomarkers for theranosis
WO2011126892A2 (en) 2010-03-30 2011-10-13 Advanced Liquid Logic, Inc. Droplet operations platform
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
BR112012025593A2 (en) 2010-04-06 2019-06-25 Caris Life Sciences Luxembourg Holdings circulating biomarkers for disease
JP2011250714A (en) * 2010-05-31 2011-12-15 Sanyo Electric Co Ltd Amplification apparatus and detecting device
WO2011156434A2 (en) 2010-06-07 2011-12-15 Firefly Bioworks, Inc. Nucleic acid detection and quantification by post-hybridization labeling and universal encoding
WO2012012090A2 (en) 2010-06-30 2012-01-26 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
US20120088691A1 (en) * 2010-10-08 2012-04-12 Gao Chen Highly multiplexed real-time pcr using encoded microbeads
EP2641097A4 (en) 2010-11-17 2016-09-07 Capacitance detection in a droplet actuator
WO2012073053A1 (en) 2010-11-30 2012-06-07 Diagon Kft. Procedure for nucleic acid-based molecular diagnostic determination of bacterial germ counts and kit for this purpose
WO2012083235A1 (en) 2010-12-16 2012-06-21 Bio-Rad Laboratories, Inc. Universal reference dye for quantitative amplification
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012250917B2 (en) 2011-05-02 2015-09-17 Advanced Liquid Logic, Inc. Molecular diagnostics platform
EP2711079B1 (en) 2011-05-09 2018-12-19 Advanced Liquid Logic, Inc. Microfluidic Feedback Using Impedance Detection
US9140635B2 (en) 2011-05-10 2015-09-22 Advanced Liquid Logic, Inc. Assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity
AU2012279420A1 (en) 2011-07-06 2014-01-30 Advanced Liquid Logic Inc Reagent storage on a droplet actuator
US8901043B2 (en) 2011-07-06 2014-12-02 Advanced Liquid Logic, Inc. Systems for and methods of hybrid pyrosequencing
WO2013009927A2 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based assays
WO2013016413A2 (en) 2011-07-25 2013-01-31 Advanced Liquid Logic Inc Droplet actuator apparatus and system
WO2013049613A1 (en) * 2011-09-29 2013-04-04 Luminex Corporation Hydrolysis probes
US20130103368A1 (en) * 2011-10-25 2013-04-25 John Lyn Farmer Automated Experimental Design For Polymerase Chain Reaction
US10731199B2 (en) 2011-11-21 2020-08-04 Advanced Liquid Logic, Inc. Glucose-6-phosphate dehydrogenase assays
US10385392B2 (en) * 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
EP4361606A2 (en) 2012-02-03 2024-05-01 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
WO2013123525A1 (en) * 2012-02-19 2013-08-22 Nvigen, Inc. Uses of ided nanostructures in nucleic acid technology
ITTO20120302A1 (en) 2012-04-05 2013-10-06 St Microelectronics Srl INTEGRATED DEVICE AND METHOD FOR CHAIN REACTION OF REAL-TIME QUANTITATIVE POLYMERASIS
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
EP2867645B1 (en) 2012-06-27 2019-06-05 Advanced Liquid Logic, Inc. Techniques and droplet actuator designs for reducing bubble formation
EP3901243A1 (en) * 2012-08-03 2021-10-27 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
CN105102630A (en) * 2012-08-16 2015-11-25 Nvs技术股份有限公司 Assay methods and systems
EP3591052B1 (en) 2012-09-24 2023-08-30 Yissum Research Development Company of The Hebrew University of Jerusalem Ltd. Restoration of the cftr function by splicing modulation
US9133510B2 (en) 2012-10-15 2015-09-15 Life Technologies Corporation Compositions, methods, systems and kits for target nucleic acid enrichment
US9863913B2 (en) 2012-10-15 2018-01-09 Advanced Liquid Logic, Inc. Digital microfluidics cartridge and system for operating a flow cell
ES2912033T3 (en) 2012-10-23 2022-05-24 Caris Science Inc Aptamers and uses thereof
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
EP2935628B1 (en) 2012-12-19 2018-03-21 Caris Life Sciences Switzerland Holdings GmbH Compositions and methods for aptamer screening
CN103881902B (en) * 2012-12-21 2016-08-10 中国科学院上海生命科学研究院 Multistage PCR response system and application thereof
EP3957743A1 (en) 2013-06-19 2022-02-23 Luminex Corporation Real-time multiplexed hydrolysis probe assay
CN111662960B (en) 2013-06-25 2024-04-12 普罗格诺西斯生物科学公司 Spatially encoded bioanalytical analysis using microfluidic devices
CN111349692B (en) * 2013-06-25 2023-10-13 C.威特沃 Method for performing polymerase chain reaction and related applications
US20160244816A1 (en) * 2013-07-15 2016-08-25 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
EP3461913B1 (en) 2013-08-09 2020-06-24 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
EP3033599A4 (en) 2013-08-13 2017-03-22 Advanced Liquid Logic, Inc. Methods of improving accuracy and precision of droplet metering using an on-actuator reservoir as the fluid input
KR20160080815A (en) 2013-08-28 2016-07-08 카리스 라이프 사이언스 스위스 홀딩스 게엠베하 Oligonucleotide probes and uses thereof
CA2920390A1 (en) 2013-08-30 2015-03-05 Illumina, Inc. Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
KR101670232B1 (en) 2013-10-28 2016-10-31 한국과학기술연구원 Porous matrix and method for producing thereof
CN106459967A (en) 2014-04-29 2017-02-22 Illumina公司 Multiplexed single cell gene expression analysis using template switch and tagmentation
EP3967771B1 (en) 2014-08-11 2023-10-04 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
DE102014221734A1 (en) * 2014-10-24 2016-04-28 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Measuring device and system for melting curve analysis of a DNA microarray, and use of a fluorescence detector array for analysis
EP3034617A1 (en) 2014-12-16 2016-06-22 Marcos Isamat Riviere Method for the simultaneous identification of multiple same-genome fragments or multiple fragments from different genomes using a PCR-technique
WO2016099999A1 (en) * 2014-12-18 2016-06-23 Ge Healthcare Uk Limited Analyte detection on a solid support by nucleic acid amplification coupled to an immunoassay
CA2979361A1 (en) 2015-03-09 2016-09-15 Caris Science, Inc. Method of preparing oligonucleotide libraries
US10576471B2 (en) 2015-03-20 2020-03-03 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
DK3901281T3 (en) 2015-04-10 2023-01-23 Spatial Transcriptomics Ab SPATIALLY SEPARATE, MULTIPLEX NUCLEIC ACID ANALYSIS OF BIOLOGICAL SAMPLES
EP3079003A1 (en) * 2015-04-10 2016-10-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Imaging method using magnetic elements and device for this purpose
WO2017004243A1 (en) 2015-06-29 2017-01-05 Caris Science, Inc. Therapeutic oligonucleotides
WO2017015133A1 (en) * 2015-07-17 2017-01-26 Life Technologies Corporation Tool for visualizing pcr results
WO2017019918A1 (en) 2015-07-28 2017-02-02 Caris Science, Inc. Targeted oligonucleotides
JP6743150B2 (en) 2015-08-28 2020-08-19 イルミナ インコーポレイテッド Single cell nucleic acid sequence analysis
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
CN105717033B (en) * 2016-01-25 2019-05-14 王博 A kind of method of flow cytometer quantitative detection of protein concentration
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
EP4339288A2 (en) 2016-03-18 2024-03-20 Caris Science, Inc. Oligonucleotide probes and uses thereof
WO2017165800A2 (en) * 2016-03-25 2017-09-28 The Board Of Regents Of The University Of Texas System Nanosensors and methods for detection of biological markers
AU2017246899B2 (en) 2016-04-07 2020-04-09 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
JP6933858B2 (en) 2016-04-07 2021-09-08 ラトガース ザ ステイト ユニバーシティー オブ ニュージャージー Multiple Nucleic Acid Assays and Reagents That Can Detect Related Alleles
KR101872803B1 (en) 2016-05-03 2018-06-29 한국과학기술연구원 Porous matrix comprising nucleic acid primer-carbon material composites and PCR using the same
AU2017271579B2 (en) 2016-05-25 2023-10-19 Caris Science, Inc. Oligonucleotide probes and uses thereof
ES2702432B2 (en) 2017-08-31 2019-08-05 Venegas Pedro Manuel Medina Method and device for nucleic acid analysis
JP7432502B2 (en) * 2017-09-22 2024-02-16 エフ． ホフマン－ラ ロシュ アーゲー Multivalent mono- or bispecific recombinant antibodies for analytical purposes
CN107796864B (en) * 2017-10-17 2020-08-14 中国科学院上海硅酸盐研究所 Application of nanoparticle coupling probe system in ctDNA high-sensitivity detection
WO2019079125A2 (en) * 2017-10-19 2019-04-25 Bio-Rad Laboratories, Inc. Digital amplification assays with unconventional and/or inverse changes in photoluminescence
WO2019089996A1 (en) * 2017-11-03 2019-05-09 University Of Washington Digital nucleic acid amplification using encoded particles
KR102653725B1 (en) 2018-01-29 2024-04-01 세인트 쥬드 칠드런즈 리써치 호스피탈, 인코포레이티드 Methods for Nucleic Acid Amplification
US20210071242A1 (en) * 2018-01-29 2021-03-11 Gen-Probe Incorporated Analytical systems and methods
WO2020014296A1 (en) * 2018-07-12 2020-01-16 Luminex Corporation Systems and methods for performing variable sample preparation and analysis processes
AU2019351130A1 (en) 2018-09-27 2021-04-08 Grail, Llc Methylation markers and targeted methylation probe panel
US11512356B2 (en) * 2018-11-08 2022-11-29 Tokitae Llc Systems and methods for particle multiplexing in droplets
CN111378645B (en) * 2018-12-27 2020-12-01 江苏金斯瑞生物科技有限公司 Gene synthesis method
EP3937780A4 (en) 2019-03-14 2022-12-07 InSilixa, Inc. Methods and systems for time-gated fluorescent-based detection
CN115210556A (en) * 2020-03-16 2022-10-18 美国西门子医学诊断股份有限公司 Optical discrimination apparatus and method adapted to monitor a reaction
IL296604A (en) 2020-03-30 2022-11-01 Illumina Inc Methods and compositions for preparing nucleic acid libraries
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
TWI747304B (en) * 2020-05-29 2021-11-21 思創影像科技股份有限公司 Microfluidic device and biological detection method using the microfluidic device
KR102365010B1 (en) * 2020-07-30 2022-02-21 주식회사 진시스템 Multiplex pcr chip and multiplex-pcr detection method using the same
WO2022177558A1 (en) * 2021-02-17 2022-08-25 Hewlett-Packard Development Company, L.P. Microfluidic nucleic acid amplification
KR20230068624A (en) 2021-11-11 2023-05-18 경북대학교 산학협력단 Apparatus for polymerase chain reaction
WO2024062237A1 (en) * 2022-09-20 2024-03-28 Ttp Plc Spatially resolved cellular profiling

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050244850A1 (en) * 2000-06-21 2005-11-03 Hiu Huang Assembly of arrays on chips segmented from wafers
WO2006028987A2 (en) * 2004-09-02 2006-03-16 Bioarray Solutions Ltd. Nucleic acid amplification with integrated multiplex detection

Family Cites Families (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5656493A (en) 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4942124A (en) 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
GB8920097D0 (en) 1989-09-06 1989-10-18 Ici Plc Amplification processes
US6787338B2 (en) 1990-06-04 2004-09-07 The University Of Utah Method for rapid thermal cycling of biological samples
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6140496A (en) 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US6703236B2 (en) 1990-11-29 2004-03-09 Applera Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
KR100236506B1 (en) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 Apparatus for polymerase chain reaction
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5719262A (en) 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
DK51092D0 (en) 1991-05-24 1992-04-15 Ole Buchardt OLIGONUCLEOTIDE ANALOGUE DESCRIBED BY PEN, MONOMERIC SYNTHONES AND PROCEDURES FOR PREPARING THEREOF, AND APPLICATIONS THEREOF
US5766855A (en) 1991-05-24 1998-06-16 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity and sequence specificity
US5714331A (en) 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5665539A (en) 1991-07-12 1997-09-09 The Regents Of The University Of California Immuno-polymerase chain reaction system for antigen detection
DE69231853T2 (en) 1991-11-07 2001-09-13 Nanotronics Inc HYBRIDIZING POLYNUCLEOTIDS CONJUGED WITH CHROMOPHORES AND FLUOROPHORS TO GENERATE A DONOR-TO-DONOR ENERGY TRANSFER SYSTEM
DE4234086A1 (en) 1992-02-05 1993-08-12 Diagen Inst Molekularbio METHOD FOR DETERMINING NUCLEIC ACID SEQUENCES AMPLIFIED IN VITRO
AU3728093A (en) 1992-02-19 1993-09-13 Public Health Research Institute Of The City Of New York, Inc., The Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
GB9211979D0 (en) 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
AU7398996A (en) 1995-10-11 1997-04-30 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
AU735440B2 (en) 1996-02-09 2001-07-05 Cornell Research Foundation Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US7235406B1 (en) 1996-04-03 2007-06-26 Applera Corporation Nucleic acid analysis device
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
GB9609441D0 (en) 1996-05-04 1996-07-10 Zeneca Ltd Process
ATE428801T1 (en) 1996-06-04 2009-05-15 Univ Utah Res Found MONITORING HYBRIDIZATION DURING PCR
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5908845A (en) 1996-10-30 1999-06-01 Segev; David Polyether nucleic acids
US6139800A (en) 1997-06-23 2000-10-31 Luminex Corporation Interlaced lasers for multiple fluorescence measurement
KR20010031140A (en) 1997-10-14 2001-04-16 루미넥스 코포레이션 Precision fluorescently dyed particles and methods of making and using same
DE69907630T2 (en) 1998-01-22 2004-02-26 Luminex Corp., Austin MICROPARTICLES WITH MULTIPLE FLUORESCENCE SIGNALS
DE19811729C2 (en) * 1998-03-18 2000-05-18 November Ag Molekulare Medizin Method and device for detecting a nucleotide sequence
JP3946444B2 (en) 1998-05-14 2007-07-18 ルミネックス コーポレイション Configuration and method for zero dead time of flow cytometer
AU3996299A (en) 1998-05-14 1999-11-29 Luminex Corporation Diode laser based measurement apparatus
WO1999058955A1 (en) 1998-05-14 1999-11-18 Luminex Corporation Multi-analyte diagnostic system and computer implemented process for same
US6818437B1 (en) 1998-05-16 2004-11-16 Applera Corporation Instrument for monitoring polymerase chain reaction of DNA
DE19824900B4 (en) 1998-06-04 2006-05-04 Roche Diagnostics Gmbh DNA detection via a strand-annealing complex
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6140054A (en) 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
CO5040034A1 (en) 1998-12-22 2001-05-29 Mcneil Ppc Inc DECORATED DEVICE FOR PERSONAL CLEANING AND METHOD AND APPARATUS TO PREPARE IT
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
AU6779200A (en) 1999-08-17 2001-03-13 Luminex Corporation Encapsulation of fluorescent particles
US7205105B2 (en) 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
JP2001257650A (en) 2000-03-14 2001-09-21 Sony Corp Information distribution system and its information distributor and receiver, and information distribution method
US9709559B2 (en) * 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
JP5213297B2 (en) 2000-08-11 2013-06-19 ユニバーシティ オブ ユタ リサーチ ファウンデーション Single label oligonucleotide probe
BRPI0206746B8 (en) 2001-01-25 2021-07-27 Luminex Molecular Diagnostics Inc composition comprising sets of molecules for use as labels or label complements, kit for classifying and identifying polynucleotides, method of analyzing a biological sample, and method for determining the presence of a target
US7226737B2 (en) 2001-01-25 2007-06-05 Luminex Molecular Diagnostics, Inc. Polynucleotides for use as tags and tag complements, manufacture and use thereof
CN1147591C (en) * 2001-02-21 2004-04-28 朱平 Method for producing polyase chain reaction gene chip
US20020177157A1 (en) * 2001-05-24 2002-11-28 Yuling Luo Pairs of nucleic acid probes with interactive signaling moieties and nucleic acid probes with enhanced hybridization efficiency and specificity
US6593091B2 (en) * 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US20070264641A1 (en) * 2001-10-15 2007-11-15 Li Alice X Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
JP2003159057A (en) * 2001-11-22 2003-06-03 Yokogawa Electric Corp Method for accelerating hybridization and apparatus for assaying biological polymer using the same method
JP3805292B2 (en) 2002-08-26 2006-08-02 日立化成工業株式会社 Electrophoresis member, method for producing the same, and capillary electrophoresis apparatus
US7157228B2 (en) * 2002-09-09 2007-01-02 Bioarray Solutions Ltd. Genetic analysis and authentication
ES2527068T3 (en) 2002-10-23 2015-01-19 University Of Utah Research Foundation Fusion analysis of amplicons with saturation dyes
ATE386141T1 (en) * 2002-12-20 2008-03-15 Stratagene Inc COMPOSITIONS AND METHODS FOR THE DETECTION OF POLYNUCLEOTIDES
US20040175704A1 (en) 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
US20050079520A1 (en) * 2003-07-21 2005-04-14 Jie Wu Multiplexed analyte detection
US7387887B2 (en) 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
WO2006007569A2 (en) * 2004-07-01 2006-01-19 Somagenics, Inc. Methods of preparation of gene-specific oligonucleotide libraries and uses thereof
JP4183256B2 (en) * 2004-08-04 2008-11-19 キヤノン株式会社 Nucleic acid amplification reaction product strand separation method, nucleic acid amplification reaction product detection method
EP1817433B1 (en) * 2004-11-10 2016-04-20 Attagene, Inc. Populations of reporter sequences and methods of their use
EP1820001A1 (en) 2004-11-12 2007-08-22 Luminex Corporation Methods and systems for positioning microspheres for imaging
TWI303927B (en) 2004-11-15 2008-12-01 Via Tech Inc Phase lock loop and control method thereof
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7456281B2 (en) 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
CN102621053B (en) 2005-09-21 2015-05-06 卢米尼克斯股份有限公司 Methods and systems for image data processing
EP2857526B1 (en) 2006-12-13 2016-08-17 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
KR20120042100A (en) 2010-10-22 2012-05-03 주식회사 씨젠 Detection of target nucleic acid sequences using dual-labeled immobilized probes on solid phase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050244850A1 (en) * 2000-06-21 2005-11-03 Hiu Huang Assembly of arrays on chips segmented from wafers
WO2006028987A2 (en) * 2004-09-02 2006-03-16 Bioarray Solutions Ltd. Nucleic acid amplification with integrated multiplex detection

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BORTOLIN SUSAN ET AL: "Analytical validation of the tag-it high-throughput microsphere-based universal array genotyping platform: Application to the multiplex detection of a panel of thrombophilia-associated single-nucleotide polymorphisms", CLINICAL CHEMISTRY,, vol. 50, no. 11, 1 November 2004 (2004-11-01), pages 2028 - 2036, XP002481140 *
FLAGELLA ET AL: "A multiplex branched DNA assay for parallel quantitative gene expression profiling", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC. NEW YORK, vol. 352, no. 1, 1 May 2006 (2006-05-01), pages 50 - 60, XP005469676, ISSN: 0003-2697 *
MARRAS ET AL: "Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes", CLINICA CHIMICA ACTA, AMSTERDAM, NL, vol. 363, no. 1-2, 1 January 2006 (2006-01-01), pages 48 - 60, XP005194900, ISSN: 0009-8981 *
MOSER ET AL: "Microsphere sedimentation arrays for multiplexed bioanalytics", ANALYTICA CHIMICA ACTA, ELSEVIER, AMSTERDAM, NL, vol. 558, no. 1-2, 3 February 2006 (2006-02-03), pages 102 - 109, XP005257909, ISSN: 0003-2670 *
STRAUB ET AL: "Automated methods for multiplexed pathogen detection", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM, NL, vol. 62, no. 3, 1 September 2005 (2005-09-01), pages 303 - 316, XP005023715, ISSN: 0167-7012 *
WILSON W J ET AL: "A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents", MOLECULAR AND CELLULAR PROBES, ACADEMIC PRESS, LONDON, GB, vol. 19, no. 2, 1 April 2005 (2005-04-01), pages 137 - 144, XP004725276, ISSN: 0890-8508 *

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