WO2008061058B1 - Flow cytometer and fluidic line assembly with multiple injection needles - Google Patents
Flow cytometer and fluidic line assembly with multiple injection needlesInfo
- Publication number
- WO2008061058B1 WO2008061058B1 PCT/US2007/084417 US2007084417W WO2008061058B1 WO 2008061058 B1 WO2008061058 B1 WO 2008061058B1 US 2007084417 W US2007084417 W US 2007084417W WO 2008061058 B1 WO2008061058 B1 WO 2008061058B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- assay
- flow
- interrogation
- injecting
- flow cell
- Prior art date
Links
- 238000002347 injection Methods 0.000 title 1
- 239000007924 injection Substances 0.000 title 1
- 239000012530 fluid Substances 0.000 claims abstract 62
- 238000003556 assay Methods 0.000 claims abstract 48
- 238000000034 method Methods 0.000 claims abstract 14
- 230000037452 priming Effects 0.000 claims abstract 3
- 230000008878 coupling Effects 0.000 claims abstract 2
- 238000010168 coupling process Methods 0.000 claims abstract 2
- 238000005859 coupling reaction Methods 0.000 claims abstract 2
- 239000002245 particle Substances 0.000 claims 5
- 238000006243 chemical reaction Methods 0.000 claims 3
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims 1
- 235000011941 Tilia x europaea Nutrition 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000005286 illumination Methods 0.000 claims 1
- 239000004571 lime Substances 0.000 claims 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
-
- G01N15/1409—
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/0318—Processes
- Y10T137/0324—With control of flow by a condition or characteristic of a fluid
- Y10T137/0329—Mixing of plural fluids of diverse characteristics or conditions
- Y10T137/0352—Controlled by pressure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/8593—Systems
- Y10T137/85978—With pump
- Y10T137/85986—Pumped fluid control
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Landscapes
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.
Claims
AMENDED CLAIMS received by the International Bureau on 28 July 2008 (28.07.2008)
1. A flow cytometer, comprising: nn interrogation flow cell; and a fluid handling system, comprising: a sheath flυidic line for supplying a sheath fluid to the interrogation flow cell; and a plurality of assay fluidic lines extending into the interrogation flow cell for introducing one or wore assay fluids into a flow of the sheath fluid within the interrogation flow cell, wherein distal ends of the plurality of assay fluidic lines are beveled.
2. The flow cytometer of claim 1 , wherein the plurality of assay flυidic lines are dimensionally configured such that when one or more assay fluids are dispensed from one or more of the plurality of assay fluidic lines at a given pressure differential with respect to the flow of sheath fluid the one or more assay fluids are substantially centrally aligned within die interrogation flow cell.
3. The flow cylometer of claim 1 , wherein the interrogation flow cell comprises a focusing section and a capillary section, and wherein the plurality of assay fluidic lines extend into the focusing section at a spaced distance of less than approximately 1.0 inch from the capillary section.
4. The flow cytometer of claim 1, further comprising: an illumination system configured to illuminate an examination zone of the interrogation flow cell; and a detection system configured to collect light emitted and/or scattered from particles passing through the examination zone,
5. The flow cytometer of claim 1, further comprising a photodetector for measuring chemiluminescence resulting from a reaction between two or more assay fluids introduced into the interrogation flow cell.
6. The flow cytometer of claim 1, wherein the fluid handling system comprises distinct pumps respectively coupled to the plurality of assay fluidic lines for respectively injecting the one or more assay fluids into the interrogation flow cell.
7. The flow cytometer of claim 1 , wherein the fluid handling system comprises a pump coupled to more than one of the plurality of assay fluidic lines for injecting at least some of the one or more assay fluids into the interrogation flow cell.
8. The flow cytometer of claim 1, wherein the fluid handling system comprises multiple pumps coupled to one of the plurality of assay fluidic lines for injecting at least some of the one or more assay fluids into the interrogation flow cell.
9. A fluidic line assembly, comprising: a base section configured for coupling to an interrogation flow cell assembly of a flow cytomctor; and a plurality of capillary lubes coupled to the base section, wherein distal ends of the plurality of capillary tubes are beveled.
11. The fluidic line assembly of claim 9, wherein the beveled surfaces of the plurality of capillary tubes face each other.
12. The fluidic lino assembly of claim 9, wherein one or more of the distal ends are beveled at angles between approximately 50 degrees and approximately 80 degrees relative to a surface of the base section to which the respective capillary tubes are coupled.
13. The fluidic line assembly of claim 9, wherein outer diameters of the capillary tubes are less than approximately 0.1 inches.
14. The fluidic line assembly of claim 9, wherein inner diameters of the capillary tubes are less than approximately 0.02 inches.
15. The fluidic line assembly of claim 9, wherein outer diameters of the capillary tubes are approximately 0,018 inches, and wherein inner diameters of the capillary tubes are approximately 0.01 inches.
16. A method, comprising: priming an interrogation flow cell of a flow cytometer with a sheath fluid; and injecting different assay fluids into a flow of the sheath fluid within the interrogation flow coll through separate fluidic lines which have beveled distal ends.
17. The method of claim 16, wherein the step of injecting the different assay fluids comprises injecting the different assay fluids intermittently while maintaining a flow of the sheath fluid within the interrogation flow cell.
18. The method of claim 16, wherein the step of injecting the different assay fluids comprises injecting the different assay fluids into the flow of the sheath fluid concurrently.
19. Tho method of claim 16, wherein the step of injecting the different assay fluids comprises injecting the different assay fluids into the flow of the sheath fluid one at a time.
20. The method of claim 19, further comprising aspirating the different assay fluids into sample loops of the flow cytometer prior to injecting the different assay fluids into the interrogation flow coll, whcroin the step of injecting the different assay fluids comprises injecting a first assay fluid from a first sample loop, wherein the step of aspirating the different assay fluids comprises aspirating a second assay fluid into a second sample loop, and wherein the steps of injecting the first assay fluid and aspirating the second assay fluid are performed at substantially the same time.
21. Tlic method of claim 16, further comprising: illuminating an examination zone of the interrogation flow cell; and measuring fluorescence of light emitted from particles passing through the examination zone, wherein the particles comprise one or more of the different assay fluids.
22, The method of claim 16, further comprising measuring chemiluminescence within the interrogation flow cell resulting from a reaction between two or more of the different assay fluids injected into the interrogation flow cell at the same time.
23, Tho fluidic line assembly of claim 9, wherein the plurality of capillary tubes are spaced apart from each other by less than approximately 0.005 inches.
24. The fluidic line assembly of claim 9, wherein the plurality of capillary tubes comprise substantial Iy equal dimensions.
25. A method, comprising: priming an interrogation flow cell of a flow cytometer with a sheath fluid; and concurrently injecting different assay fluids into a flow of the sheath fluid within the interrogation flow cell through separate non-nested fluidic lines,
26. The method of claim 25, wherein the stop of concurrently injecting the different assay fluids comprises injecting the different assay fluids intermittently while maintaining a flow of tho sheath fluid within the interrogation flow cell.
27. The method of claim 25, further comprising sequentially injecting one or more assay fluids into the flow of the sheath fluid through one or more of the separate non-nested fluidic lines cither subsequent or prior to concurrently injecting the different assay fluids into the flow of the shcalh fluid.
28. The method of claim 27, further comprising aspirating a plurality of assay fluids into sample loops of the flow cytometer, wherein the step of sequentially injecting the one or more assay fluids into the flow the sheaih fluid comprises injecting a first assay fluid from a first sample loop, wherein the step of aspirating the plurality of assay fluids comprises aspirating a second assay fluid into a second sample loop, and wherein the steps of injecting the first assay fluid and aspirating the second assay fluid are performed at substantially the same lime.
29. The method of claim 25, further comprising: illuminating an examination zone of the interrogation flow cell; and measuring fluorescence o flight emitted from particles passing through the examination zone, wherein the particles comprise one or more of the different assay fluids.
30. The method of claim 25, further comprising measuring chemiluminescence within the interrogation flow cell resulting from a reaction between two or more of the different assay fluids injected into the interrogation flow cell at the same time.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US86527706P | 2006-11-10 | 2006-11-10 | |
| US60/865,277 | 2006-11-10 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO2008061058A2 WO2008061058A2 (en) | 2008-05-22 |
| WO2008061058A3 WO2008061058A3 (en) | 2008-07-03 |
| WO2008061058B1 true WO2008061058B1 (en) | 2008-09-12 |
Family
ID=39290702
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2007/084417 WO2008061058A2 (en) | 2006-11-10 | 2007-11-12 | Flow cytometer and fluidic line assembly with multiple injection needles |
Country Status (2)
| Country | Link |
|---|---|
| US (2) | US8062609B2 (en) |
| WO (1) | WO2008061058A2 (en) |
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| US11243494B2 (en) | 2002-07-31 | 2022-02-08 | Abs Global, Inc. | Multiple laminar flow-based particle and cellular separation with laser steering |
| US20090170214A1 (en) * | 2007-12-27 | 2009-07-02 | Luminex Corporation | Luminescent Reporter Modality for Analyzing an Assay |
| US10908066B2 (en) | 2010-11-16 | 2021-02-02 | 1087 Systems, Inc. | Use of vibrational spectroscopy for microfluidic liquid measurement |
| US11668640B2 (en) | 2015-03-06 | 2023-06-06 | Inguran, Llc | Nozzle assembly for a flow cytometry system and methods of manufacture |
| CA2885234C (en) | 2012-09-19 | 2019-08-06 | Inguran, Llc | Flow cytometer nozzle tip |
| BR112015006172B1 (en) | 2012-09-19 | 2021-09-08 | Inguran, Llc | NOZZLE ASSEMBLY FOR A FLOW CYTOMETER SYSTEM |
| DE102013102438B3 (en) | 2013-03-12 | 2014-03-20 | Dionex Softron Gmbh | Flow cell for measuring e.g. permittivity of sample fluid in HPLC application, has capillary tubes arranged within protective tube to obviate unwanted mechanical application on side of capillary tubes surrounded along longitudinal direction |
| DE102013102440B3 (en) * | 2013-03-12 | 2014-05-15 | Dionex Softron Gmbh | Positioning unit for flow cell for optical detection in HPLC field, has front end facing away from flow cell formed by outer surface of flange of cell, and terminal piece whose front end is connected with light conductor and sample supply |
| DE102013102439B4 (en) | 2013-03-12 | 2021-09-02 | Dionex Softron Gmbh | Process for the production of a fluidic connection component for chromatography |
| US8961904B2 (en) | 2013-07-16 | 2015-02-24 | Premium Genetics (Uk) Ltd. | Microfluidic chip |
| US11796449B2 (en) | 2013-10-30 | 2023-10-24 | Abs Global, Inc. | Microfluidic system and method with focused energy apparatus |
| AU2014343391B2 (en) | 2013-10-30 | 2019-01-24 | Abs Global, Inc. | Microfluidic system and method with focused energy apparatus |
| US20150160246A1 (en) | 2013-12-11 | 2015-06-11 | Analiza, Inc. | Devices and methods for determining and/or isolating cells such as circulating cancer or fetal cells |
| US9709556B2 (en) | 2013-12-11 | 2017-07-18 | Analiza, Inc. | Devices and methods for determining and/or isolating circulating cancer cells |
| CN103837462B (en) * | 2014-03-03 | 2016-10-05 | 中国科学院苏州生物医学工程技术研究所 | A kind of small-sized flow cytometer liquid-way system |
| SG11201706777QA (en) | 2015-02-19 | 2017-09-28 | Premium Genetics (Uk) Ltd | Scanning infrared measurement system |
| USD868991S1 (en) | 2017-03-28 | 2019-12-03 | Becton, Dickinson And Company | Register block |
| USD869676S1 (en) | 2017-03-28 | 2019-12-10 | Becton, Dickinson And Company | Particle sorting module |
| USD864415S1 (en) | 2018-01-30 | 2019-10-22 | Becton, Dickinson And Company | Particle sorting system |
| USD876668S1 (en) | 2018-01-30 | 2020-02-25 | Becton, Dickinson And Company | Particle sorting module mount |
| USD872296S1 (en) | 2018-01-30 | 2020-01-07 | Becton, Dickinson And Company | Particle sorting module |
| USD882817S1 (en) | 2018-01-30 | 2020-04-28 | Becton, Dickinson And Company | Sample container |
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| EP3955735A4 (en) | 2019-04-18 | 2023-01-25 | ABS Global, Inc. | System and process for continuous addition of cryoprotectant |
| US11628439B2 (en) | 2020-01-13 | 2023-04-18 | Abs Global, Inc. | Single-sheath microfluidic chip |
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-
2007
- 2007-11-12 WO PCT/US2007/084417 patent/WO2008061058A2/en active Application Filing
- 2007-11-12 US US11/938,457 patent/US8062609B2/en active Active
-
2011
- 2011-10-12 US US13/271,569 patent/US8394326B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US8062609B2 (en) | 2011-11-22 |
| US20120028366A1 (en) | 2012-02-02 |
| WO2008061058A3 (en) | 2008-07-03 |
| WO2008061058A2 (en) | 2008-05-22 |
| US20080113447A1 (en) | 2008-05-15 |
| US8394326B2 (en) | 2013-03-12 |
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