WO2008030996A8 - Sirna and methods of manufacture - Google Patents

Sirna and methods of manufacture Download PDF

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Publication number
WO2008030996A8
WO2008030996A8 PCT/US2007/077810 US2007077810W WO2008030996A8 WO 2008030996 A8 WO2008030996 A8 WO 2008030996A8 US 2007077810 W US2007077810 W US 2007077810W WO 2008030996 A8 WO2008030996 A8 WO 2008030996A8
Authority
WO
WIPO (PCT)
Prior art keywords
methods
manufacture
double
sirna
stranded rna
Prior art date
Application number
PCT/US2007/077810
Other languages
French (fr)
Other versions
WO2008030996A2 (en
WO2008030996A3 (en
Inventor
N Nicole Endejann
Samuel Jotham Reich
Original Assignee
Opko Ophthalmics Llc
N Nicole Endejann
Samuel Jotham Reich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Opko Ophthalmics Llc, N Nicole Endejann, Samuel Jotham Reich filed Critical Opko Ophthalmics Llc
Priority to EP07842013A priority Critical patent/EP2076598B1/en
Priority to MX2009002462A priority patent/MX2009002462A/en
Priority to AU2007292229A priority patent/AU2007292229A1/en
Priority to JP2009527573A priority patent/JP2010502230A/en
Priority to CA002662959A priority patent/CA2662959A1/en
Publication of WO2008030996A2 publication Critical patent/WO2008030996A2/en
Publication of WO2008030996A3 publication Critical patent/WO2008030996A3/en
Publication of WO2008030996A8 publication Critical patent/WO2008030996A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)

Abstract

Double-stranded RNA of about 19 to about 25 nucleotides in length capable of regulating gene expression by RNA interference is provided. Such double-stranded RNA are particularly useful for treating disease or conditions associated with a target mRNA or gene. Methods of manufacture and methods of use of the double-stranded RNA are also provided.
PCT/US2007/077810 2006-09-08 2007-09-07 Sirna and methods of manufacture WO2008030996A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP07842013A EP2076598B1 (en) 2006-09-08 2007-09-07 Sirna and methods of manufacture
MX2009002462A MX2009002462A (en) 2006-09-08 2007-09-07 Sirna and methods of manufacture.
AU2007292229A AU2007292229A1 (en) 2006-09-08 2007-09-07 SiRNA and methods of manufacture
JP2009527573A JP2010502230A (en) 2006-09-08 2007-09-07 SIRNA and production method
CA002662959A CA2662959A1 (en) 2006-09-08 2007-09-07 Sirna and methods of manufacture

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US82495306P 2006-09-08 2006-09-08
US60/824,953 2006-09-08
US11/851,145 2007-09-06
US11/851,145 US7872118B2 (en) 2006-09-08 2007-09-06 siRNA and methods of manufacture

Publications (3)

Publication Number Publication Date
WO2008030996A2 WO2008030996A2 (en) 2008-03-13
WO2008030996A3 WO2008030996A3 (en) 2008-05-02
WO2008030996A8 true WO2008030996A8 (en) 2009-07-30

Family

ID=38983407

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/077810 WO2008030996A2 (en) 2006-09-08 2007-09-07 Sirna and methods of manufacture

Country Status (7)

Country Link
US (2) US7872118B2 (en)
EP (1) EP2076598B1 (en)
JP (1) JP2010502230A (en)
AU (1) AU2007292229A1 (en)
CA (1) CA2662959A1 (en)
MX (1) MX2009002462A (en)
WO (1) WO2008030996A2 (en)

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US7148342B2 (en) 2002-07-24 2006-12-12 The Trustees Of The University Of Pennyslvania Compositions and methods for sirna inhibition of angiogenesis
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CA2833870C (en) 2011-04-22 2020-03-10 The Regents Of The University Of California Adeno-associated virus virions with variant capsid and methods of use thereof
GB201403684D0 (en) 2014-03-03 2014-04-16 King S College London Vector
CN107073294A (en) 2014-09-05 2017-08-18 阿克赛医药公司 Use the method for targeting TYR or MMP1 exonuclease treatment aging and skin disorder
ES2908467T3 (en) 2016-04-29 2022-04-29 Adverum Biotechnologies Inc Evasion of neutralizing antibodies by a recombinant adeno-associated virus
EP3827812A1 (en) 2016-07-29 2021-06-02 The Regents of the University of California Adeno-associated virus virions with variant capsid and methods of use thereof
AU2017345470B2 (en) 2016-10-19 2023-08-03 Adverum Biotechnologies, Inc. Modified AAV capsids and uses thereof
US20230190956A1 (en) 2020-04-24 2023-06-22 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for preventing induction of immune responses to the transduced cells expressing a transgene product after ocular gene therapy
KR20240014477A (en) 2021-05-28 2024-02-01 상하이 레제네리드 테라피즈 컴퍼니 리미티드 Recombinant adeno-associated virus with variant capsid and applications thereof

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Also Published As

Publication number Publication date
US20090061487A1 (en) 2009-03-05
US7872118B2 (en) 2011-01-18
US20110143400A1 (en) 2011-06-16
WO2008030996A2 (en) 2008-03-13
CA2662959A1 (en) 2008-03-13
WO2008030996A3 (en) 2008-05-02
JP2010502230A (en) 2010-01-28
EP2076598B1 (en) 2012-05-23
MX2009002462A (en) 2009-07-31
AU2007292229A1 (en) 2008-03-13
EP2076598A2 (en) 2009-07-08

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