WO2007137119B1 - Chip-based flow cytometer type systems for analyzing fluorescently tagged particles - Google Patents

Chip-based flow cytometer type systems for analyzing fluorescently tagged particles

Info

Publication number
WO2007137119B1
WO2007137119B1 PCT/US2007/069147 US2007069147W WO2007137119B1 WO 2007137119 B1 WO2007137119 B1 WO 2007137119B1 US 2007069147 W US2007069147 W US 2007069147W WO 2007137119 B1 WO2007137119 B1 WO 2007137119B1
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
channel
flow
sample
chip
Prior art date
Application number
PCT/US2007/069147
Other languages
French (fr)
Other versions
WO2007137119A2 (en
WO2007137119A3 (en
Inventor
Adam Richard Schilffarth
William R Deicher
John C Carrano
Jesse C Phillips
Original Assignee
Luminex Corp
Adam Richard Schilffarth
William R Deicher
John C Carrano
Jesse C Phillips
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Luminex Corp, Adam Richard Schilffarth, William R Deicher, John C Carrano, Jesse C Phillips filed Critical Luminex Corp
Priority to EP07797550A priority Critical patent/EP2021766A2/en
Priority to CA002657970A priority patent/CA2657970A1/en
Priority to JP2009511233A priority patent/JP2009537826A/en
Priority to CN2007800174923A priority patent/CN101454653B/en
Publication of WO2007137119A2 publication Critical patent/WO2007137119A2/en
Publication of WO2007137119A3 publication Critical patent/WO2007137119A3/en
Publication of WO2007137119B1 publication Critical patent/WO2007137119B1/en
Priority to HK09111287.0A priority patent/HK1133298A1/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484Electro-optical investigation, e.g. flow cytometers microstructural devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Abstract

Portable systems for processing and analyzing biological or environmental samples as well as different configurations of chip-based flow cytometers are provided. The portable systems include an automated assay preparation module configured to process a sample into a fluid assay with fluorescently tagged particles and a microfluidic analysis module coupled to the fluid assay module, wherein the microfluidic analysis module includes a chip-based flow cytometer.

Claims

29
AMENDED CLAIMS received by the International Bureau on 28 April 2008 (28.04.2008)
1. Λ portable system for processing and analyzing biological or environmental samples, comprising: an automated assay preparation module configured to process a sample into a fluid assay with fluorcsccntly tagged particles; and a røicrofluidic analysis module coupled to the automated assay preparation module, wherein the πiiorofluidio analysis module comprises a chip-based flow cytomctcr having; a noπ- removable substrate upon which a channel is arranged for routing a fluid stream comprising the fluid assay through the flow cytometcr; and an illumination subsystem comprising a light source system and an optical system collectively configured to direct light toward an interrogation region of the channel.
2. The portable system of claim 1, wherein the chip-based flow cytomctcr comprises: a first input conduit for receiving the fluid assay; a second distinct conduit for receiving a sheath fluid; and a fluid flow chamber coupled to the first and second input conduits, wherein the fluid flow chamber is configured to generate the fluid stream by confining the fluid assay within the sheath fluid.
3. The poi table system of claim 2, wherein the fluid flow chamber is configured to generate the fluid stream with the fluid assay having a first dimension of up to approximately SO microns in a vertical direction perpendicular to the flow of the fluid slruam and a second dimension of up to approximately 25 microns in a horizontal direction perpendicular to the flow of the fluid stream.
4. The portable system of claim 2, wherein the chip-based flow cylomcter further comprises a measurement subsystem comprising: a collection system configured to gather fluorescence emitted from the fluorcsccntly tagged particles; and an examination system coupled for analyzing the collected fluorescence. 30
5. Tlic portable system of claim 4, wherein the first conduit, second conduit, fluid flow chain be. , channel, raid at least soma optics of the collection system arc fixedly arranged upon the MOii -removable substrate.
6. The portable system of claim 5, wherein the illumination subsystem and the measurement subsystem arc fixedly arranged relative to each other and the non-removable substrate.
7. The portable system o f claim 4, wherein the collection system comprises an aspherical mirror to direct light emitted from the fluorcscently tagged particles to a detector of the collection system.
S. The portable system of claim 1, wherein the assay preparation module is configured to process the sample into a fluid assay with fluorescenily tagged magnetic particles, and wherein the flow cylometcr comprises a means for inducing a magnetic field along at least a portion of the channel such that the fluoresccntly tagged magnetic particles flow within a predetermined region of the fluid stream.
9, The portable system of claim 1, wherein the channel, light source system, and optical system ore arranged perpendicular to each other.
10. The portable system of claim 4, wherein the light source system is configured such that individual light sources within the light source system direct light at different spots within the i nloi rogation region, and wherein the collection system is configured such that the fluorescent light emitted from the fluoresccntly tagged particles at each of the different spots is collected by a different detector of the collection system,
1 1. TUo portable system of claim 10, wherein at least one of the individual light sources emits light within a different wavelength range than another of the individual light sources.
12, The portable system of claim 103 wherein the collection system comprises a different set of collodion optics to direct tlio light emitted at each of the different spots to a different detector of the collection system.
13. Λ system for analyzing a fluid sample, comprising: si channel for routing a fluid sample comprising magnetic parttcjes through the system; ,i means for inducing a magnetic field along at least a portion of the channel such that the magnetic particles flow within a predetermined region of the fluid sample; an illumination subsystem comprising a light source system and an optical system collectively configured to direct light toward an interrogation region of the channel; and a measurement subsystem comprising a collection system configured Io gather fluorescence emitted from the magnetic particles and further comprising an examination system for analyzing the collected fluorescence.
14. The system of claim 13, wherein the means for inducing the magnetic field comprises a solenoid coil wrapped around the fluid channel.
15. The system of claim 13, fiuthcr comprising a first input conduit for receiving the fluid sample; a second di&lϊπct conduit for receiving a sheath, fluid; and a fluid flow chamber coupled to the first and second input conduits, wherein the fluid flow chamber is configured to confine the sample fluid within the sheath fluid, and wherein the fluid flow chamber is further coupled to the channel such that the channel receives the amalgamated fluids.
16. 7 lie system of claim 15, wherein the means for inducing the magnetic field is configured to induce the magnetic field along a portion of the channel extending between the fluid flow chamber and through the interrogation region.
17. ThO system of claim 15, wherein the fluid flow chamber is configured to narrow the diameter of the sample fluid from the input conduit by less than a factor of 10.
18. Λ chip-based flow cylometer, comprising: a first input conduit for receiving a fluid sample comprising fluorescently tagged particles; a second distinct conduit for receiving a sheath fluid; 32 a fluid (low chamber coupled to the first and second input conduits, wherein the fluid flow chamber is configured to generate a fluid stream with the sample fluid confined within the sheath fluid, and wherein the confined sample fluid comprises a diameter a first dimension of up to approximately SO microns in a vertical direction perpendicular to the flow of the fluid stream and a second dimension of πp to approximately 25 microns in a horizontal direction perpendicular to tlie flow of the fluid stream; a channel coupled to the fluid flow chamber for routing the fluid stream through the flow cytometer; an illumination subsystem comprising a light source system and an optical system collectively configured to direct light toward an mtcrrogaticm region of the channel; and a measurement subsystem comprising a collection system configured to gather fluorescence emitted from the fluorescently tagged particles and further comprising an examination system for analyzing the collected fluorescence; and whciein the first conduit, second conduit, fluid flow chamber, channel, and at least some optics of the collection system are fixedly arranged upon a non-removable subsliate included within the chip-based flow cytomoter.
20. The chip-based flow cytometer of claim 18, wherein the illumination subsystem and the measurement subsystem are fixedly arranged lelative to each other and the substrate,
21. A system for analyzing a fluid sample, comprising: a channel for routing a fluid sample comprising fluorescently tagged particles through the system; flu illumination subsystem comprising a light source system and an optical system collectively configured to direct light toward an interrogation region of the channel; and a measurement subsystem comprising an aspherical mirror configured to gather fluorescence emitted from the fluorescently tagged particles and further comprising an examination system for analyzing the collected fluorescence. 33
22. The system of claim 21, wherein the aspherical mirror is coated with an anti-re flεclivo coaling.
2S1 . The system of claim 22, wherein the anti-reflective coating is configured to reflect fluorescent emissions and not scattered light.
24. The system of claim 22, wherein the anti-reflective coating is configured to reflect sen tiered light and fluorescent emissions,
25. Λ chip-based flow cytometer, comprising: a channel for routing a fluid sample comprising fluoresceπtly tagged particles through the flow cytometer; an illumination subsystem comprising a light source system and an optical system collectively configured such that individual light sources within the light source system direct light toward different spots within the interrogation region; and
Ά measurement subsystem comprising a collection system configured such that the fluorescent light emitted from the fluorescenlly tagged particles at each of the different spots is collected by a different detector of the collection system and further comprising an examination system for analyzing the collected fluorescence,
26. 'flic chip-based flow cytometer of claim 25, wherein light source system comprises more limn 3 different light sources.
27. The portable system of claim 25, wherein at least one of the individual light sources emits light within a different wavelength range than another of the individual light sources.
28. The portable system of claim 25, wherein the collection system comprises a different set of collection optics Io direct the light emitted at each of the different spots to a different detector of lhc collection system.
PCT/US2007/069147 2006-05-17 2007-05-17 Chip-based flow cytometer type systems for analyzing fluorescently tagged particles WO2007137119A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP07797550A EP2021766A2 (en) 2006-05-17 2007-05-17 Chip-based flow cytometer type systems for analyzing fluorescently tagged particles
CA002657970A CA2657970A1 (en) 2006-05-17 2007-05-17 Chip-based flow cytometer type systems for analyzing fluorescently tagged particles
JP2009511233A JP2009537826A (en) 2006-05-17 2007-05-17 Chip-based flow cytometer-type system for analyzing fluorescently labeled particles
CN2007800174923A CN101454653B (en) 2006-05-17 2007-05-17 Chip-based flow cytometer type systems for analyzing fluorescently tagged particles
HK09111287.0A HK1133298A1 (en) 2006-05-17 2009-12-02 Chip-based flow cytometer type systems for analyzing fluorescently tagged particles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74748306P 2006-05-17 2006-05-17
US60/747,483 2006-05-17

Publications (3)

Publication Number Publication Date
WO2007137119A2 WO2007137119A2 (en) 2007-11-29
WO2007137119A3 WO2007137119A3 (en) 2008-05-08
WO2007137119B1 true WO2007137119B1 (en) 2008-07-10

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/069147 WO2007137119A2 (en) 2006-05-17 2007-05-17 Chip-based flow cytometer type systems for analyzing fluorescently tagged particles

Country Status (8)

Country Link
US (2) US9810707B2 (en)
EP (1) EP2021766A2 (en)
JP (1) JP2009537826A (en)
KR (1) KR20090027643A (en)
CN (1) CN101454653B (en)
CA (1) CA2657970A1 (en)
HK (1) HK1133298A1 (en)
WO (1) WO2007137119A2 (en)

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US20070269345A1 (en) 2007-11-22
JP2009537826A (en) 2009-10-29
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KR20090027643A (en) 2009-03-17
US9810707B2 (en) 2017-11-07
CN101454653B (en) 2013-01-16
HK1133298A1 (en) 2010-03-19
CA2657970A1 (en) 2007-11-29
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CN101454653A (en) 2009-06-10
US20180100872A1 (en) 2018-04-12

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