WO2007035684A3 - Method for quantitative detection of short rna molecules - Google Patents

Method for quantitative detection of short rna molecules Download PDF

Info

Publication number
WO2007035684A3
WO2007035684A3 PCT/US2006/036380 US2006036380W WO2007035684A3 WO 2007035684 A3 WO2007035684 A3 WO 2007035684A3 US 2006036380 W US2006036380 W US 2006036380W WO 2007035684 A3 WO2007035684 A3 WO 2007035684A3
Authority
WO
WIPO (PCT)
Prior art keywords
short rna
rna molecules
quantitative detection
detection
quantitation
Prior art date
Application number
PCT/US2006/036380
Other languages
French (fr)
Other versions
WO2007035684A2 (en
Inventor
Vladimir I Slepnev
Original Assignee
Primera Biosystems Inc
Vladimir I Slepnev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Primera Biosystems Inc, Vladimir I Slepnev filed Critical Primera Biosystems Inc
Publication of WO2007035684A2 publication Critical patent/WO2007035684A2/en
Publication of WO2007035684A3 publication Critical patent/WO2007035684A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Abstract

Described herein are approaches to the detection and quantitation of short RNAs in a biological sample. The methods permit the detection and quantitation of individual species of short RNA in a nucleic acid sample, both singly and in a multiplex format that permits the determination of expression levels for two or more target short RNAs in a single reaction.
PCT/US2006/036380 2005-09-16 2006-09-18 Method for quantitative detection of short rna molecules WO2007035684A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71763805P 2005-09-16 2005-09-16
US60/717,638 2005-09-16

Publications (2)

Publication Number Publication Date
WO2007035684A2 WO2007035684A2 (en) 2007-03-29
WO2007035684A3 true WO2007035684A3 (en) 2007-07-12

Family

ID=37889444

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/036380 WO2007035684A2 (en) 2005-09-16 2006-09-18 Method for quantitative detection of short rna molecules

Country Status (2)

Country Link
US (1) US20070077582A1 (en)
WO (1) WO2007035684A2 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1772522A1 (en) * 2005-10-04 2007-04-11 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Control of preservation by biomarkers
CA2657576C (en) 2006-07-14 2023-10-31 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
US20090023151A1 (en) * 2006-08-30 2009-01-22 Dawson Elliott P Method For The Labeling And Detection Of Small Polynucleotides
ATE544869T1 (en) * 2006-08-30 2012-02-15 Bioventures Inc METHOD AND SUBSTANCES FOR ISOLATION AND DETECTION OF SMALL POLYNUCLEOTIDES
US8415096B2 (en) 2007-05-23 2013-04-09 University Of South Florida Micro-RNAs modulating immunity and inflammation
EP2187896B1 (en) 2007-07-31 2016-01-13 The Board of Regents of The University of Texas System Inhibitors of mir-499 and mir-208b for use in the treatment of pathologic cardiac hypertrophy, heart failure or myocardial infarction
US20110027798A1 (en) * 2008-03-31 2011-02-03 The Ohio State University Research Foundation Hybridization Quantitation Method for Modified Micro-RNA and -DNA Based Oligonucleotides
CA2744004A1 (en) 2008-11-25 2010-06-17 Gen-Probe Incorporated Compositions and methods for detecting small rnas, and uses thereof
WO2010075659A1 (en) * 2009-01-05 2010-07-08 Wang Xiaolong Method for amplifying oligonucleotide and small rna by using polymerase-endonuclease chain reaction
NZ594365A (en) 2009-02-04 2013-03-28 Univ Texas Dual targeting of miR-208a or miR-208b and miR-499 in the treatment of cardiac disorders or musculoskeletal disorders
FR2950360B1 (en) * 2009-09-22 2011-11-25 Centre Nat Rech Scient CELL IMAGING METHOD FOR VISUALIZATION OF MICROARN BIOGENESIS IN CELLS
US20120003639A1 (en) 2010-04-27 2012-01-05 Prelude, Inc. Cancer biomarkers and methods of use thereof
US9428799B2 (en) 2011-07-25 2016-08-30 Bioinventors & Entrepreneurs Network Llc Method for determining an allele profile of nucleic acid
US8932989B2 (en) 2011-07-25 2015-01-13 Bioinventors & Entrepreneurs Network, Llc Sieving of nucleic acid samples
US9133567B2 (en) 2011-07-25 2015-09-15 Bioinventors & Entrepreneurs Network Llc Method for determining an attribute profile of biological samples
US10351899B2 (en) * 2013-09-25 2019-07-16 Bio-ID Diagnostics Inc. Methods for detecting nucleic acid fragments
WO2015085194A1 (en) * 2013-12-06 2015-06-11 The Broad Institute, Inc. Enhanced methods of ribonucleic acid hybridization
WO2015195949A2 (en) * 2014-06-18 2015-12-23 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
CN104120184B (en) * 2014-07-28 2016-04-13 成都诺恩生物科技有限公司 A kind of method utilizing amplification of DNA fragments length polymorphism to measure Short interfering RNA
EP3183364B1 (en) * 2014-08-19 2018-11-28 Bio-Rad Laboratories, Inc. Rna amplification methods
EP3227687A4 (en) 2014-12-05 2018-10-24 Prelude, Inc. Dcis recurrence and invasive breast cancer
GB201501930D0 (en) 2015-02-05 2015-03-25 Univ London Queen Mary Biomarkers for pancreatic cancer
GB201517028D0 (en) * 2015-09-25 2015-11-11 Univ London Queen Mary Novel biomarkers for pancreatic cancer
US11401558B2 (en) 2015-12-18 2022-08-02 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
JP2022500504A (en) 2018-09-14 2022-01-04 プレリュード コーポレーションPrelude Corporation How to choose treatment for subjects at risk of invasive breast cancer
KR102141312B1 (en) * 2019-04-19 2020-08-04 주식회사 제노헬릭스 Small RNA-primed Xenosensor module amplification mediated small RNA detection method
US20210198725A1 (en) * 2019-12-31 2021-07-01 Xenohelix Co., Ltd Method of detecting small rna

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040110191A1 (en) * 2001-01-31 2004-06-10 Winkler Matthew M. Comparative analysis of nucleic acids using population tagging
US20040259128A1 (en) * 2003-03-24 2004-12-23 Glenn Kawasaki Methods and compositions for detecting the presence of target nucleic acids in a sample

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5710628A (en) * 1994-12-12 1998-01-20 Visible Genetics Inc. Automated electrophoresis and fluorescence detection apparatus and method
JP4000605B2 (en) * 1996-07-24 2007-10-31 株式会社日立製作所 DNA sample preparation device and electrophoretic analyzer using the same
US6207031B1 (en) * 1997-09-15 2001-03-27 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
US7115365B2 (en) * 1998-12-07 2006-10-03 Olympus Optical Co., Ltd. Method of analyzing a target nucleic acid
US6618679B2 (en) * 2000-01-28 2003-09-09 Althea Technologies, Inc. Methods for analysis of gene expression
US20020006617A1 (en) * 2000-02-07 2002-01-17 Jian-Bing Fan Nucleic acid detection methods using universal priming
US20050037362A1 (en) * 2003-08-11 2005-02-17 Eppendorf Array Technologies, S.A. Detection and quantification of siRNA on microarrays
US20050260648A1 (en) * 2004-04-06 2005-11-24 Huffel Christophe V Method for the determination of cellular transcriptional
US8192937B2 (en) * 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040110191A1 (en) * 2001-01-31 2004-06-10 Winkler Matthew M. Comparative analysis of nucleic acids using population tagging
US20040259128A1 (en) * 2003-03-24 2004-12-23 Glenn Kawasaki Methods and compositions for detecting the presence of target nucleic acids in a sample

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SCHMITTGEN T.D. ET AL.: "A high-throughput method to monitor the expression of microRNA precursors", NUCLEIC ACIDS RESEARCH, vol. 32, no. 4, E43, 2004, pages 1 - 10, XP002364907 *

Also Published As

Publication number Publication date
WO2007035684A2 (en) 2007-03-29
US20070077582A1 (en) 2007-04-05

Similar Documents

Publication Publication Date Title
WO2007035684A3 (en) Method for quantitative detection of short rna molecules
WO2007024798A3 (en) Apparatus, system, and method using immiscible-fluid-discrete-volumes
WO2010026488A3 (en) Methods and kits for nucleic acid sequencing
WO2003062462A3 (en) Method for isolating nucleic acids and protein from a single sample
WO2010083250A3 (en) Single-cell nucleic acid analysis
WO2010062913A3 (en) Methods and systems for analysis of sequencing data
EP1994142A4 (en) Methods and compositions for the rapid isolation of small rna molecules
WO2008008515A3 (en) Methods and compositions for detecting rare cells from a biological sample
WO2007146819A3 (en) Methods for identifying and using snp panels
WO2009152928A3 (en) Method for the quantitative analysis of nucleic acids, markers therefor and their use
WO2009076485A3 (en) Sequencing of nucleic acids
WO2009086608A3 (en) Method and kit to perform a pcr amplification and micro- array detection in the same medium and/or same chamber
WO2007044974A3 (en) Absolute pcr quantification
WO2009060847A1 (en) Method and kit for preparation of sample for use in nucleic acid amplification
WO2008076842A3 (en) Sequencing methods
WO2009092035A3 (en) Methods and compositions for the analysis of biological molecules
WO2008005321A3 (en) Systems and methods for real-time pcr
WO2007098427A3 (en) Massively multiplexed sequencing
WO2006076567A3 (en) Microfluidic rare cell detection device
WO2007039319A3 (en) Dna microarray for rapid identification of candida albicans in blood cultures
WO2007065025A3 (en) Method of dna analysis using micro/nanochannel
WO2003064605A3 (en) Crude biological derivatives competent for nucleic acid detection
WO2006050499A3 (en) Single step detection assay
WO2004053105A3 (en) Direct SNP detection with unamplified DNA
WO2004081228A3 (en) Real time gene expression profiling

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 06824999

Country of ref document: EP

Kind code of ref document: A2