WO2007027353A3 - Detection of proteases secreted from pathogenic microorganisms - Google Patents

Detection of proteases secreted from pathogenic microorganisms Download PDF

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Publication number
WO2007027353A3
WO2007027353A3 PCT/US2006/030107 US2006030107W WO2007027353A3 WO 2007027353 A3 WO2007027353 A3 WO 2007027353A3 US 2006030107 W US2006030107 W US 2006030107W WO 2007027353 A3 WO2007027353 A3 WO 2007027353A3
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
chromatographic medium
compound
detection
pathogenic microorganisms
Prior art date
Application number
PCT/US2006/030107
Other languages
French (fr)
Other versions
WO2007027353A2 (en
Inventor
Xuedong Song
Original Assignee
Kimberly Clark Co
Xuedong Song
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/217,097 external-priority patent/US7504235B2/en
Application filed by Kimberly Clark Co, Xuedong Song filed Critical Kimberly Clark Co
Priority to EP06789202A priority Critical patent/EP1920253A2/en
Publication of WO2007027353A2 publication Critical patent/WO2007027353A2/en
Publication of WO2007027353A3 publication Critical patent/WO2007027353A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A diagnostic test kit for detecting the presence or absence of a protease (e.g., aminopeptidase) within a test sample is provided. The test kit comprises a substrate that is capable of being cleaved in the presence of the protease to release a compound. The kit also comprises a lateral flow device that comprises a chromatographic medium. The chromatographic medium defines a detection zone within which is contained a first reagent (e.g., diazonium ion) that is capable of reacting with the compound to form a second reagent (e.g., aromatic azo compound). The second reagent exhibits a color that is different than the color of the first reagent. The lateral flow device also includes an absorbent material located adjacent to the chromatographic medium, the absorbent material receiving the test sample after flowing through the chromatographic medium.
PCT/US2006/030107 2005-08-31 2006-08-01 Detection of proteases secreted from pathogenic microorganisms WO2007027353A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06789202A EP1920253A2 (en) 2005-08-31 2006-08-01 Detection of proteases secreted from pathogenic microorganisms

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11/217,097 US7504235B2 (en) 2005-08-31 2005-08-31 Enzyme detection technique
US11/217,097 2005-08-31
US11/364,810 US7575887B2 (en) 2005-08-31 2006-02-28 Detection of proteases secreted from pathogenic microorganisms
US11/364,810 2006-02-28

Publications (2)

Publication Number Publication Date
WO2007027353A2 WO2007027353A2 (en) 2007-03-08
WO2007027353A3 true WO2007027353A3 (en) 2007-06-07

Family

ID=37809345

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/030107 WO2007027353A2 (en) 2005-08-31 2006-08-01 Detection of proteases secreted from pathogenic microorganisms

Country Status (2)

Country Link
EP (1) EP1920253A2 (en)
WO (1) WO2007027353A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7935538B2 (en) 2006-12-15 2011-05-03 Kimberly-Clark Worldwide, Inc. Indicator immobilization on assay devices

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7504235B2 (en) * 2005-08-31 2009-03-17 Kimberly-Clark Worldwide, Inc. Enzyme detection technique
US20080269707A1 (en) * 2007-04-30 2008-10-30 Kimberly-Clark Worldwide, Inc. Lateral Flow Device for Attachment to an Absorbent Article

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4806423A (en) * 1984-04-06 1989-02-21 Miles Inc. Agents and process for the detection of esterolytic and/or proteolytic enzymes
US5571684A (en) * 1994-11-07 1996-11-05 Litmus Concepts, Inc. Assay for proline iminopeptidase and other hydrolytic activities
WO2005057214A1 (en) * 2003-11-21 2005-06-23 Kimberly-Clark Worldwide, Inc. Method for extending the dynamic detection range of assay devices
WO2005098020A1 (en) * 2004-04-01 2005-10-20 Binax, Inc. Assay method for determining mucosal neutrophil counts in neutropenia patients
WO2007027234A2 (en) * 2005-08-31 2007-03-08 Kimberly-Clark Worldwide, Inc. Enzyme detection technique

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306642B1 (en) * 1997-11-24 2001-10-23 Quidel Corporation Enzyme substrate delivery and product registration in one step enzyme immunoassays
US7906276B2 (en) * 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US7521226B2 (en) * 2004-06-30 2009-04-21 Kimberly-Clark Worldwide, Inc. One-step enzymatic and amine detection technique

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4806423A (en) * 1984-04-06 1989-02-21 Miles Inc. Agents and process for the detection of esterolytic and/or proteolytic enzymes
US5571684A (en) * 1994-11-07 1996-11-05 Litmus Concepts, Inc. Assay for proline iminopeptidase and other hydrolytic activities
WO2005057214A1 (en) * 2003-11-21 2005-06-23 Kimberly-Clark Worldwide, Inc. Method for extending the dynamic detection range of assay devices
WO2005098020A1 (en) * 2004-04-01 2005-10-20 Binax, Inc. Assay method for determining mucosal neutrophil counts in neutropenia patients
WO2007027234A2 (en) * 2005-08-31 2007-03-08 Kimberly-Clark Worldwide, Inc. Enzyme detection technique

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1920253A2 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7935538B2 (en) 2006-12-15 2011-05-03 Kimberly-Clark Worldwide, Inc. Indicator immobilization on assay devices

Also Published As

Publication number Publication date
WO2007027353A2 (en) 2007-03-08
EP1920253A2 (en) 2008-05-14

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