WO2007016090A3 - Methods and apparatus for reducing protein content in sperm cell extenders - Google Patents

Methods and apparatus for reducing protein content in sperm cell extenders Download PDF

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Publication number
WO2007016090A3
WO2007016090A3 PCT/US2006/028846 US2006028846W WO2007016090A3 WO 2007016090 A3 WO2007016090 A3 WO 2007016090A3 US 2006028846 W US2006028846 W US 2006028846W WO 2007016090 A3 WO2007016090 A3 WO 2007016090A3
Authority
WO
WIPO (PCT)
Prior art keywords
sperm cell
protein content
techniques
reducing protein
extenders
Prior art date
Application number
PCT/US2006/028846
Other languages
French (fr)
Other versions
WO2007016090A2 (en
Inventor
John L Schenk
Original Assignee
Xy Inc
John L Schenk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to NZ566129A priority Critical patent/NZ566129A/en
Application filed by Xy Inc, John L Schenk filed Critical Xy Inc
Priority to AU2006275984A priority patent/AU2006275984B2/en
Priority to DK06800316.9T priority patent/DK1924139T3/en
Priority to JP2008524067A priority patent/JP5394737B2/en
Priority to EP06800316.9A priority patent/EP1924139B1/en
Priority to BRPI0622294-3A priority patent/BRPI0622294B1/en
Priority to ES06800316.9T priority patent/ES2538979T3/en
Priority to CA2619951A priority patent/CA2619951C/en
Priority to BRPI0614912-0A priority patent/BRPI0614912B1/en
Priority to MX2008001276A priority patent/MX2008001276A/en
Publication of WO2007016090A2 publication Critical patent/WO2007016090A2/en
Publication of WO2007016090A3 publication Critical patent/WO2007016090A3/en
Priority to IL189638A priority patent/IL189638A0/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

Abstract

The inventive technology relates to methods and apparatus for reducing protein content in sperm cell extenders and may include one or more of the following features: techniques for reducing protein content in a sperm cell extender; techniques for reducing protein content in a cryoprotectant-containing B fraction of a sperm cell extender; techniques for preparing sperm cell extenders that do not require clarification; techniques for preparing low density gradient sperm cell extenders suitable for centrifugation; techniques for reducing protein content between individual steps in preparing a sperm cell extender, and techniques for establishing novel values of reduced protein content in sperm cell extenders.
PCT/US2006/028846 2005-07-29 2006-07-24 Methods and apparatus for reducing protein content in sperm cell extenders WO2007016090A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
BRPI0622294-3A BRPI0622294B1 (en) 2005-07-29 2006-07-24 MIXING OF INCIPIENTS
AU2006275984A AU2006275984B2 (en) 2005-07-29 2006-07-24 Methods and apparatus for reducing protein content in sperm cell extenders
DK06800316.9T DK1924139T3 (en) 2005-07-29 2006-07-24 Methods and apparatus for reducing the protein content of sædcellestrækmidler
JP2008524067A JP5394737B2 (en) 2005-07-29 2006-07-24 Method and apparatus for reducing protein content in sperm cell extenders
EP06800316.9A EP1924139B1 (en) 2005-07-29 2006-07-24 Methods and apparatus for reducing protein content in sperm cell extenders
NZ566129A NZ566129A (en) 2005-07-29 2006-07-24 Methods for freezing sorted sperm cells comprising a protein containing sperm cell extender and a protein free cryoprotectant before freezing
ES06800316.9T ES2538979T3 (en) 2005-07-29 2006-07-24 Method and apparatus for reducing protein content in sperm cell extenders
MX2008001276A MX2008001276A (en) 2005-07-29 2006-07-24 Methods and apparatus for reducing protein content in sperm cell extenders.
BRPI0614912-0A BRPI0614912B1 (en) 2005-07-29 2006-07-24 METHODS AND APPARATUS FOR REDUCING PROTEIN CONTENT IN SPERM CELL EXTENSORS
CA2619951A CA2619951C (en) 2005-07-29 2006-07-24 Methods and apparatus for reducing protein content in sperm cell extenders
IL189638A IL189638A0 (en) 2005-07-29 2008-02-20 Methods and apparatus for reducing protein content in sperm cell extenders

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US70400105P 2005-07-29 2005-07-29
US60/704,001 2005-07-29
US11/219,607 US7618770B2 (en) 2005-07-29 2005-09-02 Methods and apparatus for reducing protein content in sperm cell extenders
US11/219,607 2005-09-02

Publications (2)

Publication Number Publication Date
WO2007016090A2 WO2007016090A2 (en) 2007-02-08
WO2007016090A3 true WO2007016090A3 (en) 2007-11-29

Family

ID=37694763

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/028846 WO2007016090A2 (en) 2005-07-29 2006-07-24 Methods and apparatus for reducing protein content in sperm cell extenders

Country Status (15)

Country Link
US (2) US7618770B2 (en)
EP (1) EP1924139B1 (en)
JP (2) JP5394737B2 (en)
AR (1) AR054180A1 (en)
AU (1) AU2006275984B2 (en)
BR (2) BRPI0622294B1 (en)
CA (1) CA2619951C (en)
DK (1) DK1924139T3 (en)
ES (1) ES2538979T3 (en)
IL (1) IL189638A0 (en)
MX (1) MX2008001276A (en)
NZ (3) NZ592267A (en)
PT (1) PT1924139E (en)
UY (1) UY29708A1 (en)
WO (1) WO2007016090A2 (en)

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US7208265B1 (en) * 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
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US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
AU3768902A (en) 2000-11-29 2002-06-11 Xy Inc System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
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US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
AU2003265362B2 (en) * 2002-08-01 2009-11-05 Xy, Llc. Low pressure sperm cell separation system
AU2003265471B2 (en) 2002-08-15 2009-08-06 Xy, Llc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2308417T3 (en) 2003-03-28 2016-07-04 Inguran Llc Apparatus and methods for obtaining sorted particles
AU2004242121B2 (en) * 2003-05-15 2010-06-24 Xy, Llc. Efficient haploid cell sorting for flow cytometer systems
EP2801363B1 (en) 2004-03-29 2018-02-21 Inguran, LLC Process for storing sorted spermatozoa
CA2574499C (en) 2004-07-22 2016-11-29 Monsanto Technology Llc Process for enriching a population of sperm cells
US7618770B2 (en) * 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
WO2011143741A1 (en) * 2010-05-15 2011-11-24 Hopital Maisonneuve-Rosemont Extenders for mammalian sperm processing and preservation
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
JP7212886B2 (en) * 2019-03-22 2023-01-26 国立研究開発法人産業技術総合研究所 Method for producing sperm liquid for fertilization and method for producing frozen sperm straw
CN110343615B (en) * 2019-08-27 2023-05-23 浙江大学 Culture dish for sperm-egg in-vitro combined fertilization and use method

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