WO2006113957A8 - Production of recombinant proteins by autoproteolytic cleavage of a fusion protein - Google Patents
Production of recombinant proteins by autoproteolytic cleavage of a fusion proteinInfo
- Publication number
- WO2006113957A8 WO2006113957A8 PCT/AT2006/000165 AT2006000165W WO2006113957A8 WO 2006113957 A8 WO2006113957 A8 WO 2006113957A8 AT 2006000165 W AT2006000165 W AT 2006000165W WO 2006113957 A8 WO2006113957 A8 WO 2006113957A8
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- polypeptide
- interest
- fusion
- inclusion bodies
- heterologous
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
- C07K17/02—Peptides being immobilised on, or in, an organic carrier
- C07K17/06—Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
- C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
Abstract
Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008508019A JP2008538897A (en) | 2005-04-26 | 2006-04-25 | Production of recombinant protein by self-proteolytic cleavage of fusion protein |
ES06721223T ES2399031T3 (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by self-protection cleavage of a fusion protein |
CN200680014330XA CN101198700B (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
US11/919,346 US7829319B2 (en) | 2005-04-26 | 2006-04-25 | Glutamic acid-modified classical swine fever virus autoproteases Npro |
DK06721223.3T DK1874932T3 (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
CA2605140A CA2605140C (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
KR1020077024875A KR101374149B1 (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
EP06721223A EP1874932B1 (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
SI200631498T SI1874932T1 (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
AU2006239721A AU2006239721B2 (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0508434.8 | 2005-04-26 | ||
GBGB0508435.5A GB0508435D0 (en) | 2005-04-26 | 2005-04-26 | Organic compounds |
GBGB0508434.8A GB0508434D0 (en) | 2005-04-26 | 2005-04-26 | Organic compounds |
GB0508435.5 | 2005-04-26 | ||
GB0605379A GB0605379D0 (en) | 2006-03-16 | 2006-03-16 | Organic compounds |
GB0605379.7 | 2006-03-16 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2006113957A2 WO2006113957A2 (en) | 2006-11-02 |
WO2006113957A8 true WO2006113957A8 (en) | 2007-02-22 |
WO2006113957A3 WO2006113957A3 (en) | 2007-06-07 |
Family
ID=36796250
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/AT2006/000165 WO2006113957A2 (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
PCT/AT2006/000168 WO2006113959A2 (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
PCT/AT2006/000167 WO2006113958A2 (en) | 2005-04-26 | 2006-04-25 | Affinity ligands |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/AT2006/000168 WO2006113959A2 (en) | 2005-04-26 | 2006-04-25 | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
PCT/AT2006/000167 WO2006113958A2 (en) | 2005-04-26 | 2006-04-25 | Affinity ligands |
Country Status (15)
Country | Link |
---|---|
US (4) | US7829319B2 (en) |
EP (5) | EP1874932B1 (en) |
JP (5) | JP2008539170A (en) |
KR (3) | KR101374149B1 (en) |
CN (1) | CN103333252A (en) |
AT (1) | ATE461936T1 (en) |
AU (3) | AU2006239721B2 (en) |
CA (3) | CA2605140C (en) |
DE (1) | DE602006013111D1 (en) |
DK (3) | DK1874825T3 (en) |
ES (2) | ES2399031T3 (en) |
PL (1) | PL1874825T3 (en) |
PT (2) | PT1874825E (en) |
SI (2) | SI1874932T1 (en) |
WO (3) | WO2006113957A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102141353B1 (en) | 2012-12-19 | 2020-08-06 | 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 | Method for producing a recombinant protein of interest |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PL1874825T3 (en) * | 2005-04-26 | 2011-04-29 | Boehringer Ingelheim Rcv Gmbh | Affinity ligands |
CA2687170C (en) | 2007-05-17 | 2019-06-25 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Method for producing a recombinant protein on a manufacturing scale |
JP2011502125A (en) * | 2007-10-31 | 2011-01-20 | ディフュージョン・ファーマシューティカルズ・エルエルシー | A new class of treatments that promote small molecule diffusion |
EP2130912A1 (en) * | 2008-06-04 | 2009-12-09 | Institut für Viruskrankeiten und Immunprophylaxe | Pestivirus replicons providing an RNA-based viral vector system |
US20120171184A1 (en) | 2010-12-31 | 2012-07-05 | Lajos Szente | Cellular hydration compositions |
CN104946626A (en) | 2011-01-12 | 2015-09-30 | 积水医疗株式会社 | Eluent for ion-exchange chromatography, and method of analyzing nucleic acid chains |
KR102047650B1 (en) | 2011-01-12 | 2019-11-22 | 세키스이 메디칼 가부시키가이샤 | Method for detecting single nucleotide polymorphisms |
US9339526B2 (en) * | 2011-01-17 | 2016-05-17 | University Of Manitoba | Methods for treating disorders that involve immunoglobulin A |
EP2684951A1 (en) | 2012-07-13 | 2014-01-15 | Sandoz Ag | Method for producing a recombinant protein of interest |
EP2746391A1 (en) | 2012-12-19 | 2014-06-25 | Sandoz Ag | Method for producing a recombinant protein of interest |
UY35874A (en) | 2013-12-12 | 2015-07-31 | Novartis Ag | A PROCESS FOR THE PREPARATION OF A COMPOSITION OF PEGILATED PROTEINS |
ES2871624T3 (en) * | 2014-12-19 | 2021-10-29 | Hoffmann La Roche | Microbial transglutaminases, substrates therefor, and procedures for their use |
US11054425B2 (en) | 2014-12-19 | 2021-07-06 | Roche Sequencing Solutions, Inc. | System and method for identification and characterization of transglutaminase species |
CN107636005B (en) * | 2015-03-13 | 2021-07-16 | 百时美施贵宝公司 | Removal of impurities by alkaline washing in chromatographic processes |
CN104844685B (en) * | 2015-06-12 | 2018-10-16 | 中国科学院植物研究所 | A kind of denatured antigen affinity purification antibody method |
RU2619217C1 (en) * | 2015-12-04 | 2017-05-12 | Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") | Temperaturically intelligent mutant intein for the insoluble expression of the precursor of the target protein |
KR101830792B1 (en) | 2016-01-27 | 2018-02-21 | 건국대학교 산학협력단 | Method for producing antimicrobial peptide using insoluble fusion protein containing antimicrobial peptide |
EP3643781A4 (en) * | 2017-06-23 | 2021-07-14 | Zhuhai Essex Bio-pharmaceutical Co., Ltd. | Method for producing soluble recombinant human-basic fibroblast growth factor (rh-bfgf) |
DE102018200602A1 (en) | 2018-01-15 | 2019-07-18 | Technische Universität München | Biological synthesis of amino acid chains for the production of peptides and proteins |
CN110343183B (en) * | 2018-04-03 | 2023-05-12 | 点斗基因科技(南京)有限公司 | Recombinant expression vector and construction method and application thereof |
CN111019962A (en) * | 2019-12-18 | 2020-04-17 | 南京理工大学 | SOD-ELP fusion protein and preparation method thereof |
EP3904525A1 (en) | 2020-04-27 | 2021-11-03 | Kutzner, Christoph | Fusion polypeptides for target peptide production |
CN114539425B (en) * | 2022-02-25 | 2023-04-28 | 湖南中晟全肽生化有限公司 | Method for improving biological expression of linear polypeptide |
EP4265636A1 (en) | 2022-04-19 | 2023-10-25 | mk2 Biotechnologies GmbH | Preparation of target peptides and proteins |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5649200A (en) * | 1993-01-08 | 1997-07-15 | Atria Software, Inc. | Dynamic rule-based version control system |
AU6171194A (en) | 1993-02-05 | 1994-08-29 | Affymax Technologies N.V. | Receptor-binding antiproliferative peptides |
US5837684A (en) * | 1995-06-07 | 1998-11-17 | Nycomed Imaging As | Peptides |
US5778395A (en) * | 1995-10-23 | 1998-07-07 | Stac, Inc. | System for backing up files from disk volumes on multiple nodes of a computer network |
WO1998015572A1 (en) * | 1996-10-09 | 1998-04-16 | Akzo Nobel N.V. | A composition comprising an immobilised, acylated peptide |
WO1999010483A2 (en) * | 1997-08-22 | 1999-03-04 | Roche Diagnostics Gmbh | Protease precursors that can be autocatalytically activated and their use |
DE19740310A1 (en) * | 1997-09-13 | 1999-04-01 | Octapharma Ag | Peptide with affinity for coagulation factor VIII |
DE19819843A1 (en) | 1998-05-05 | 1999-11-11 | Biotechnolog Forschung Gmbh | Metal chelate binding peptides |
US5985836A (en) * | 1998-07-31 | 1999-11-16 | Bayer Corporation | Alpha-1 proteinase inhibitor binding peptides |
SK288290B6 (en) * | 1999-08-09 | 2015-07-01 | Sandoz Ag | Vector for expression of heterologous proteins in bacterial host cell, bacterial host cell and process for production of desired heterologous polypeptide |
SK287488B6 (en) | 1999-08-09 | 2010-11-08 | Sandoz Ag | Process for the recombinant production of a heterologous polypeptide with the autoproteolytic activity |
JP4202574B2 (en) * | 2000-01-11 | 2008-12-24 | Aspion株式会社 | Peptides with affinity for gp120 |
WO2001081375A2 (en) * | 2000-04-24 | 2001-11-01 | Yale University | Dna & protein binding miniature proteins |
EP1367961B1 (en) * | 2001-02-06 | 2008-12-31 | Massachusetts Institute Of Technology | Peptide scaffold encapsulation of tissue cells and uses thereof |
JP4369662B2 (en) | 2001-04-26 | 2009-11-25 | アビディア インコーポレイテッド | Combinatorial library of monomer domains |
US7043485B2 (en) * | 2002-03-19 | 2006-05-09 | Network Appliance, Inc. | System and method for storage of snapshot metadata in a remote file |
US7185027B2 (en) * | 2002-07-11 | 2007-02-27 | Cisco Technology, Inc. | Evolving entries within persistent stores in a scalable infrastructure environment |
US7794980B2 (en) | 2002-11-12 | 2010-09-14 | Yeda Research And Development Co. Ltd. | Chimeric autoprocessing polypeptides and uses thereof |
US7074615B2 (en) * | 2003-08-15 | 2006-07-11 | Becton, Dickinson And Company | Peptides for enhanced cell attachment and cell growth |
US7467386B2 (en) * | 2004-01-16 | 2008-12-16 | International Business Machines Corporation | Parameter passing of data structures where API and corresponding stored procedure are different versions/releases |
US7448585B2 (en) * | 2004-12-16 | 2008-11-11 | Sunway, Incorporated | Keyboard support assembly |
PL1874825T3 (en) * | 2005-04-26 | 2011-04-29 | Boehringer Ingelheim Rcv Gmbh | Affinity ligands |
EP2197908A2 (en) * | 2007-09-27 | 2010-06-23 | Dako Denmark A/S | Mhc multimers in tuberculosis diagnostics, vaccine and therapeutics |
-
2006
- 2006-04-25 PL PL06721225T patent/PL1874825T3/en unknown
- 2006-04-25 ES ES06721223T patent/ES2399031T3/en active Active
- 2006-04-25 WO PCT/AT2006/000165 patent/WO2006113957A2/en not_active Application Discontinuation
- 2006-04-25 SI SI200631498T patent/SI1874932T1/en unknown
- 2006-04-25 ES ES06721225T patent/ES2342100T3/en active Active
- 2006-04-25 EP EP06721223A patent/EP1874932B1/en not_active Not-in-force
- 2006-04-25 WO PCT/AT2006/000168 patent/WO2006113959A2/en active Application Filing
- 2006-04-25 EP EP06721226.6A patent/EP1874933B1/en not_active Not-in-force
- 2006-04-25 CN CN2013102335999A patent/CN103333252A/en active Pending
- 2006-04-25 KR KR1020077024875A patent/KR101374149B1/en active IP Right Grant
- 2006-04-25 AU AU2006239721A patent/AU2006239721B2/en active Active
- 2006-04-25 EP EP11160999A patent/EP2348053A3/en not_active Withdrawn
- 2006-04-25 PT PT06721225T patent/PT1874825E/en unknown
- 2006-04-25 SI SI200630687T patent/SI1874825T1/en unknown
- 2006-04-25 CA CA2605140A patent/CA2605140C/en active Active
- 2006-04-25 AU AU2006239722A patent/AU2006239722B2/en not_active Ceased
- 2006-04-25 DE DE602006013111T patent/DE602006013111D1/en active Active
- 2006-04-25 KR KR1020077027161A patent/KR101289728B1/en not_active IP Right Cessation
- 2006-04-25 EP EP06721225A patent/EP1874825B1/en not_active Not-in-force
- 2006-04-25 US US11/919,346 patent/US7829319B2/en active Active
- 2006-04-25 US US11/919,257 patent/US8163890B2/en active Active
- 2006-04-25 CA CA002605149A patent/CA2605149A1/en not_active Abandoned
- 2006-04-25 US US11/919,258 patent/US8058410B2/en not_active Expired - Fee Related
- 2006-04-25 DK DK06721225.8T patent/DK1874825T3/en active
- 2006-04-25 PT PT67212233T patent/PT1874932E/en unknown
- 2006-04-25 EP EP11167687.0A patent/EP2366719B1/en active Active
- 2006-04-25 JP JP2008508020A patent/JP2008539170A/en active Pending
- 2006-04-25 CA CA002605145A patent/CA2605145A1/en not_active Abandoned
- 2006-04-25 KR KR1020077024887A patent/KR20080036545A/en not_active Application Discontinuation
- 2006-04-25 DK DK06721226.6T patent/DK1874933T3/en active
- 2006-04-25 DK DK06721223.3T patent/DK1874932T3/en active
- 2006-04-25 AT AT06721225T patent/ATE461936T1/en active
- 2006-04-25 JP JP2008508019A patent/JP2008538897A/en active Pending
- 2006-04-25 AU AU2006239723A patent/AU2006239723B2/en not_active Ceased
- 2006-04-25 JP JP2008508021A patent/JP5101492B2/en not_active Expired - Fee Related
- 2006-04-25 WO PCT/AT2006/000167 patent/WO2006113958A2/en not_active Application Discontinuation
-
2012
- 2012-01-11 US US13/348,607 patent/US8372959B2/en not_active Expired - Fee Related
- 2012-10-05 JP JP2012223547A patent/JP5909172B2/en active Active
-
2015
- 2015-08-20 JP JP2015163036A patent/JP2015226547A/en not_active Withdrawn
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102141353B1 (en) | 2012-12-19 | 2020-08-06 | 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 | Method for producing a recombinant protein of interest |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2006113957A3 (en) | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein | |
TW200732472A (en) | Method for the recombinant expression of a polypeptide | |
Alanen et al. | Efficient export of human growth hormone, interferon α2b and antibody fragments to the periplasm by the Escherichia coli Tat pathway in the absence of prior disulfide bond formation | |
EP1422237A3 (en) | Methods for the recombinant production of antifusogenic peptides | |
WO2002090495A8 (en) | Rapidly cleavable sumo fusion protein expression system for difficult to express proteins | |
KR20170085129A (en) | Fusion partners for peptide production | |
CA2472477A1 (en) | Methods and compositions for protein expression and purification | |
NO20090451L (en) | Process for Preparation of Conjugates of Insulin-Like Growth Factor-1 and Poly (Ethylene Glucole) | |
WO1994018332A3 (en) | Anthrax toxin fusion proteins and uses thereof | |
WO2003100021A3 (en) | Methods and dna constructs for high yield production of polypeptides | |
NZ508863A (en) | Generation of multiple gene products from chimeric fusion proteins by cleavage with ubiquitous endoproteases | |
MXPA02001424A (en) | Production of proteins. | |
CN102533839A (en) | Method for performing fusion expression on protein containing disulfide bond | |
Kaar et al. | Refolding of Npro fusion proteins | |
Fine et al. | Cloning, expression, and purification of functional Sec11a and Sec11b, type I signal peptidases of the archaeon Haloferax volcanii | |
JP2003506093A5 (en) | ||
NZ589806A (en) | Variant hedgehog-interacting protein (hhip1) and methods and uses thereof | |
HUP0203051A2 (en) | Production of proteins by autoproteolytic cleavage | |
WO2010013789A1 (en) | Protein production method, fusion protein, and antiserum | |
JP2016518855A (en) | Fusion protease | |
WO2003100022A3 (en) | Methods and dna constructs for high yield production of polypeptides | |
WO2005001099A8 (en) | Protein expression system | |
Fang et al. | Expression of recombinant Chinese bovine enterokinase catalytic subunit in P. pastoris and its purification and characterization | |
KR20130020208A (en) | Method for producing soluble foreign protein using specific intracellular cleavage system | |
Reyes-Ruiz et al. | Proteins in a DNA world: expression systems for their study |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2006239721 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2605140 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 7911/DELNP/2007 Country of ref document: IN |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2008508019 Country of ref document: JP Ref document number: 2006721223 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 11919346 Country of ref document: US Ref document number: 200680014330.X Country of ref document: CN Ref document number: 1020077024875 Country of ref document: KR |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: DE |
|
ENP | Entry into the national phase |
Ref document number: 2006239721 Country of ref document: AU Date of ref document: 20060425 Kind code of ref document: A |
|
WWP | Wipo information: published in national office |
Ref document number: 2006239721 Country of ref document: AU |
|
NENP | Non-entry into the national phase |
Ref country code: RU |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: RU |
|
WWP | Wipo information: published in national office |
Ref document number: 2006721223 Country of ref document: EP |