WO2006103450A3 - Electrochemical assay - Google Patents

Electrochemical assay Download PDF

Info

Publication number
WO2006103450A3
WO2006103450A3 PCT/GB2006/001177 GB2006001177W WO2006103450A3 WO 2006103450 A3 WO2006103450 A3 WO 2006103450A3 GB 2006001177 W GB2006001177 W GB 2006001177W WO 2006103450 A3 WO2006103450 A3 WO 2006103450A3
Authority
WO
WIPO (PCT)
Prior art keywords
species
binding reagent
electrochemical assay
cleaved
fluid sample
Prior art date
Application number
PCT/GB2006/001177
Other languages
French (fr)
Other versions
WO2006103450A2 (en
Inventor
David Edward Williams
Philip Lowe
Christopher John Slevin
Anne-Cecile Herve
Steven John Carlisle
Alan Thomson
Original Assignee
Inverness Medical Switzerland
David Edward Williams
Philip Lowe
Christopher John Slevin
Anne-Cecile Herve
Steven John Carlisle
Alan Thomson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inverness Medical Switzerland, David Edward Williams, Philip Lowe, Christopher John Slevin, Anne-Cecile Herve, Steven John Carlisle, Alan Thomson filed Critical Inverness Medical Switzerland
Priority to US11/887,393 priority Critical patent/US20090035876A1/en
Priority to EP06726583A priority patent/EP1866645A2/en
Publication of WO2006103450A2 publication Critical patent/WO2006103450A2/en
Publication of WO2006103450A3 publication Critical patent/WO2006103450A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

Abstract

A method of determining the presence or amount of analyte in a fluid sample, which comprises: (a) contacting a fluid sample with a binding reagent that comprises a plurality of cleavable species and wherein said species, when cleaved, are detectable using electrochemical means; (b) separating any binding reagent-analyte complex that forms from the unbound binding reagent; (c) cleaving the cleavable species from the immobilized binding reagent- analyte complex; and (d) detecting the cleaved species using electrochemical means.
PCT/GB2006/001177 2005-03-31 2006-03-31 Electrochemical assay WO2006103450A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US11/887,393 US20090035876A1 (en) 2005-03-31 2006-03-31 Electrochemical Assay
EP06726583A EP1866645A2 (en) 2005-03-31 2006-03-31 Electrochemical assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US66656205P 2005-03-31 2005-03-31
US60/666,562 2005-03-31

Publications (2)

Publication Number Publication Date
WO2006103450A2 WO2006103450A2 (en) 2006-10-05
WO2006103450A3 true WO2006103450A3 (en) 2006-12-14

Family

ID=36951244

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2006/001177 WO2006103450A2 (en) 2005-03-31 2006-03-31 Electrochemical assay

Country Status (4)

Country Link
US (1) US20090035876A1 (en)
EP (1) EP1866645A2 (en)
CN (1) CN101184996A (en)
WO (1) WO2006103450A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101470098B (en) * 2007-12-27 2013-01-09 中国石油化工股份有限公司 Method for measuring content of ferrocene and derivatives thereof in oil products
EP2390664B1 (en) * 2010-05-25 2013-04-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method for electrochemical detection of binding reactions
US11529610B2 (en) 2012-09-17 2022-12-20 W.R. Grace & Co.-Conn. Functionalized particulate support material and methods of making and using the same
RU2015114330A (en) 2012-09-17 2016-11-10 У.Р. Грейс Энд Ко.-Конн. CHROMATOGRAPHIC MEDIA AND DEVICES
ES2887110T3 (en) 2014-01-16 2021-12-21 Grace W R & Co Affinity Chromatography Media and Chromatography Devices
ES2929099T3 (en) 2014-05-02 2022-11-24 Grace W R & Co Functionalized support material and methods of manufacturing and use of functionalized support material
WO2015184442A1 (en) * 2014-05-30 2015-12-03 Georgia State University Research Foundation Electrochemical methods and compounds for the detection of enzymes
US9678070B2 (en) 2015-04-29 2017-06-13 Church & Dwight Co., Inc. Method and apparatus for electrochemical detection
JP2018517559A (en) 2015-06-05 2018-07-05 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ Adsorbing bioprocess clarifier and method for producing and using the same

Citations (2)

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Publication number Priority date Publication date Assignee Title
US4945045A (en) * 1984-07-06 1990-07-31 Serono Diagnostics Ltd. Electrochemical methods of assay
WO2003076649A1 (en) * 2002-03-05 2003-09-18 Aclara Biosciences, Inc. Multiplex analysis using membrane-bound sensitizers

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EP0078636B2 (en) * 1981-10-23 1997-04-02 MediSense, Inc. Sensor for components of a liquid mixture
US5427912A (en) * 1993-08-27 1995-06-27 Boehringer Mannheim Corporation Electrochemical enzymatic complementation immunoassay
US6589736B1 (en) * 1994-11-22 2003-07-08 The Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US6312893B1 (en) * 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US7037654B2 (en) * 1999-04-30 2006-05-02 Aclara Biosciences, Inc. Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
AU2002239780A1 (en) * 2000-10-25 2002-06-03 Tufts University Polymeric microspheres
US7045367B2 (en) * 2001-03-23 2006-05-16 Michigan Molecular Institute Nano-scaled dendrimer-based colorimetric biosensors
AU2002365115A1 (en) * 2001-07-20 2003-09-02 North Carolina State University Light addressable electrochemical detection of duplex structures
GB0119001D0 (en) * 2001-08-03 2001-09-26 Amersham Pharm Biotech Uk Ltd Use of dendrimers and poly-branched molecules to enhance signal in fluorescent assay systems
US20030198960A1 (en) * 2002-04-01 2003-10-23 Wenhong Fan Signal amplifying targeted reporters for biological and chemical sensor applications
US20040018515A1 (en) * 2002-04-03 2004-01-29 Diener John L. Compositions selective for adenosine diphosphate and methods of using same
JP4101801B2 (en) * 2002-06-24 2008-06-18 シーメンス アクチエンゲゼルシャフト Biosensor array and method of use thereof
US7473535B2 (en) * 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
US7138462B2 (en) * 2003-08-22 2006-11-21 Los Alamos National Security, Llc Functionalized polymers for binding to solutes in aqueous solutions
WO2005085850A1 (en) * 2004-02-27 2005-09-15 Tianxin Wang Methods for multiplexed analyte detection

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945045A (en) * 1984-07-06 1990-07-31 Serono Diagnostics Ltd. Electrochemical methods of assay
WO2003076649A1 (en) * 2002-03-05 2003-09-18 Aclara Biosciences, Inc. Multiplex analysis using membrane-bound sensitizers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHOI M J ET AL: "LABELING DIGOXIN ANTIBODY WITH COLLOIDAL GOLD AND FERROCENE FOR ITSUSE IN A MEMBRANE IMMUNOSTRIP AND IMMUNOSENSOR", MICROCHEMICAL JOURNAL, NEW YORK, NY, US, vol. 63, September 1999 (1999-09-01), pages 92 - 99, XP000924842, ISSN: 0026-265X *

Also Published As

Publication number Publication date
US20090035876A1 (en) 2009-02-05
EP1866645A2 (en) 2007-12-19
WO2006103450A2 (en) 2006-10-05
CN101184996A (en) 2008-05-21

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