WO2006102233A3 - Method and apparatus for imaging target components in a biological sample using permanent magnets - Google Patents

Method and apparatus for imaging target components in a biological sample using permanent magnets Download PDF

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Publication number
WO2006102233A3
WO2006102233A3 PCT/US2006/010026 US2006010026W WO2006102233A3 WO 2006102233 A3 WO2006102233 A3 WO 2006102233A3 US 2006010026 W US2006010026 W US 2006010026W WO 2006102233 A3 WO2006102233 A3 WO 2006102233A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
target
labelled
biological sample
sample
Prior art date
Application number
PCT/US2006/010026
Other languages
French (fr)
Other versions
WO2006102233A2 (en
Inventor
Arjan Tibbe
Leon W M M Terstappen
Original Assignee
Immunivest Corp
Arjan Tibbe
Leon W M M Terstappen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunivest Corp, Arjan Tibbe, Leon W M M Terstappen filed Critical Immunivest Corp
Publication of WO2006102233A2 publication Critical patent/WO2006102233A2/en
Publication of WO2006102233A3 publication Critical patent/WO2006102233A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations

Abstract

A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, all cells in a biological sample are fluorescently labelled, but only the target cells are also magnetically labelled. A small, permanent magnet is inserted directly into the chamber containing the labelled sample. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The cells are illuminated and the images of the fluorescent light emitted by the target cells are captured by a CCD camera. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample.
PCT/US2006/010026 2005-03-18 2006-03-20 Method and apparatus for imaging target components in a biological sample using permanent magnets WO2006102233A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59419805P 2005-03-18 2005-03-18
US60/594,198 2005-03-18

Publications (2)

Publication Number Publication Date
WO2006102233A2 WO2006102233A2 (en) 2006-09-28
WO2006102233A3 true WO2006102233A3 (en) 2008-09-04

Family

ID=37024478

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/010026 WO2006102233A2 (en) 2005-03-18 2006-03-20 Method and apparatus for imaging target components in a biological sample using permanent magnets

Country Status (1)

Country Link
WO (1) WO2006102233A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2374686T3 (en) 2007-05-14 2012-02-21 Historx, Inc. SEPARATION IN COMPARTMENTS BY CHARACTERIZATION OF PIXEL USING CLOTHING OF IMAGE DATA.
CA2604317C (en) 2007-08-06 2017-02-28 Historx, Inc. Methods and system for validating sample images for quantitative immunoassays
US8110101B2 (en) 2007-08-30 2012-02-07 Veridex, Llc Method and apparatus for imaging target components in a biological sample using permanent magnets
US7828968B2 (en) * 2007-08-30 2010-11-09 Veridex, Llc Method and apparatus for imaging target components in a biological sample using permanent magnets
CN202011883U (en) 2007-12-12 2011-10-19 里兰斯坦福初级大学理事会 Device for capturing and separating target cell
JP5551702B2 (en) 2008-09-16 2014-07-16 ヒストロックス，インコーポレイテッド． Reproducible quantification of biomarker expression
GB0820300D0 (en) 2008-11-06 2008-12-17 Univ Dundee Apparatus and method for the detection of cells
WO2010091304A1 (en) * 2009-02-05 2010-08-12 Veridex, Llc A filter method for separating ferrofluids in a biological sample
WO2023180465A1 (en) * 2022-03-23 2023-09-28 University Of Twente A magnetic micro-needle to isolate single immunomagnetically labeled cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5374531A (en) * 1993-03-22 1994-12-20 Zynaxis, Inc. Immunoassay for determination of cells
US6409925B1 (en) * 1998-02-06 2002-06-25 Bio-Magnetics Ltd. Device and system for transfer of material
US6500549B1 (en) * 1998-10-13 2002-12-31 Gambro Ab Biocompatible polymer film

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5374531A (en) * 1993-03-22 1994-12-20 Zynaxis, Inc. Immunoassay for determination of cells
US6409925B1 (en) * 1998-02-06 2002-06-25 Bio-Magnetics Ltd. Device and system for transfer of material
US6500549B1 (en) * 1998-10-13 2002-12-31 Gambro Ab Biocompatible polymer film

Also Published As

Publication number Publication date
WO2006102233A2 (en) 2006-09-28

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