WO2006089192A3 - Devices and methods for identifying genomic dna of organisms - Google Patents

Devices and methods for identifying genomic dna of organisms Download PDF

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Publication number
WO2006089192A3
WO2006089192A3 PCT/US2006/005780 US2006005780W WO2006089192A3 WO 2006089192 A3 WO2006089192 A3 WO 2006089192A3 US 2006005780 W US2006005780 W US 2006005780W WO 2006089192 A3 WO2006089192 A3 WO 2006089192A3
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WO
WIPO (PCT)
Prior art keywords
methods
genomic material
organism
identifying
organisms
Prior art date
Application number
PCT/US2006/005780
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French (fr)
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WO2006089192A2 (en
Inventor
Toru Takahashi
Hiroshi Inoue
Original Assignee
Canon Us Life Sciences Inc
Toru Takahashi
Hiroshi Inoue
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Canon Us Life Sciences Inc, Toru Takahashi, Hiroshi Inoue filed Critical Canon Us Life Sciences Inc
Priority to CN200680013055XA priority Critical patent/CN101163800B/en
Priority to AU2006214085A priority patent/AU2006214085A1/en
Priority to JP2007556351A priority patent/JP4982387B2/en
Priority to AT06735442T priority patent/ATE538213T1/en
Priority to CA002598520A priority patent/CA2598520A1/en
Priority to EP06735442A priority patent/EP1871903B1/en
Publication of WO2006089192A2 publication Critical patent/WO2006089192A2/en
Publication of WO2006089192A3 publication Critical patent/WO2006089192A3/en

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    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
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    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
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    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/139—Random amplification polymorphism detection [RAPD]
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/173—Nucleic acid detection characterized by the use of physical, structural and functional properties staining/intercalating agent, e.g. ethidium bromide
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
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Abstract

The invention provides an apparatus that can be used in methods of preparing, amplifying, detecting, and/or optionally selecting for further analysis the genomic material from an organism for the rapid detection and/or classification of an organism in a sample (e.g., screening for, identifying, quantifying, and/or optionally further analyzing, e.g., sequencing, the genomic material of the organism). The invention further provides methods of using the apparatus, e.g., in combination with novel SGP primers for improved use in waveform-profiling methods of DNA amplification. It is an object of the invention to provide an apparatus for fully automated analysis of genomic material, and multiple methods of using the apparatus that are beneficial to society, e.g., the apparatus may be used in methods of screening for, identifying, quantifying, and/or selecting genomic material for further analysis (e.g., sequencing) in relation to monitoring a source for the presence of contaminating organisms.
PCT/US2006/005780 2005-02-18 2006-02-17 Devices and methods for identifying genomic dna of organisms WO2006089192A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN200680013055XA CN101163800B (en) 2005-02-18 2006-02-17 Devices and methods for monitoring genomic DNA of organisms
AU2006214085A AU2006214085A1 (en) 2005-02-18 2006-02-17 Devices and methods for identifying genomic DNA of organisms
JP2007556351A JP4982387B2 (en) 2005-02-18 2006-02-17 Device and method for identifying genomic DNA of microorganisms
AT06735442T ATE538213T1 (en) 2005-02-18 2006-02-17 DEVICE AND METHOD FOR IDENTIFYING GENOMIC DNA OF ORGANISMS
CA002598520A CA2598520A1 (en) 2005-02-18 2006-02-17 Devices and methods for identifying genomic dna of organisms
EP06735442A EP1871903B1 (en) 2005-02-18 2006-02-17 Devices and methods for identifying genomic dna of organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65397805P 2005-02-18 2005-02-18
US60/653,978 2005-02-18

Publications (2)

Publication Number Publication Date
WO2006089192A2 WO2006089192A2 (en) 2006-08-24
WO2006089192A3 true WO2006089192A3 (en) 2006-10-12

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PCT/US2006/005780 WO2006089192A2 (en) 2005-02-18 2006-02-17 Devices and methods for identifying genomic dna of organisms

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US (2) US7604938B2 (en)
EP (1) EP1871903B1 (en)
JP (1) JP4982387B2 (en)
KR (1) KR101544351B1 (en)
CN (1) CN101163800B (en)
AT (1) ATE538213T1 (en)
AU (1) AU2006214085A1 (en)
CA (1) CA2598520A1 (en)
WO (1) WO2006089192A2 (en)

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