WO2006087603A3 - Isoelectric particles and uses as electrophoretic tags for multiple analyte detection using capillary isoelectric focusing - Google Patents

Isoelectric particles and uses as electrophoretic tags for multiple analyte detection using capillary isoelectric focusing Download PDF

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Publication number
WO2006087603A3
WO2006087603A3 PCT/IB2005/004090 IB2005004090W WO2006087603A3 WO 2006087603 A3 WO2006087603 A3 WO 2006087603A3 IB 2005004090 W IB2005004090 W IB 2005004090W WO 2006087603 A3 WO2006087603 A3 WO 2006087603A3
Authority
WO
WIPO (PCT)
Prior art keywords
isoelectric
particles
analyte detection
multiple analyte
electrophoretic tags
Prior art date
Application number
PCT/IB2005/004090
Other languages
French (fr)
Other versions
WO2006087603A2 (en
Inventor
Branko Kozulic
Original Assignee
Gilman Michael G
Branko Kozulic
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gilman Michael G, Branko Kozulic filed Critical Gilman Michael G
Priority to GBGB0624182.2A priority Critical patent/GB0624182D0/en
Publication of WO2006087603A2 publication Critical patent/WO2006087603A2/en
Priority to HR20060424A priority patent/HRP20060424A2/en
Publication of WO2006087603A3 publication Critical patent/WO2006087603A3/en
Priority to US12/220,042 priority patent/US8076163B2/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis

Abstract

A plurality of particles of from about 5 gun to 100 µ:m possessing predetermined isoelectric points in the pH range from about 2.5 to i l is used in a method of detection of a plurality of analytes, wherein the isoelectric particles of each isoelectric point further contain a label and a member of a binding pair capable of interacting with a selected analyte. The particles that formed specific binding pairs are recovered and separated by isoelectric focusing, followed by the detection of the labels associated with the particles. A flow cytometer may be used as a detector of the isoelectric particles.
PCT/IB2005/004090 2004-05-07 2005-05-03 Isoelectric particles and uses as electrophoretic tags for multiple analyte detection using capillary isoelectric focusing WO2006087603A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
GBGB0624182.2A GB0624182D0 (en) 2004-05-07 2005-05-03 Isoelectric particles and uses thereof
HR20060424A HRP20060424A2 (en) 2004-05-07 2006-12-06 Isoelectric particles and uses thereof
US12/220,042 US8076163B2 (en) 2004-05-07 2008-07-21 Isoelectric particles and uses therefore

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/840,347 US7407816B2 (en) 2004-05-07 2004-05-07 Isoelectric particles and uses thereof
US10/840,347 2004-05-07

Publications (2)

Publication Number Publication Date
WO2006087603A2 WO2006087603A2 (en) 2006-08-24
WO2006087603A3 true WO2006087603A3 (en) 2007-04-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2005/004090 WO2006087603A2 (en) 2004-05-07 2005-05-03 Isoelectric particles and uses as electrophoretic tags for multiple analyte detection using capillary isoelectric focusing

Country Status (4)

Country Link
US (2) US7407816B2 (en)
GB (1) GB0624182D0 (en)
HR (1) HRP20060424A2 (en)
WO (1) WO2006087603A2 (en)

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WO2009144621A1 (en) * 2008-05-27 2009-12-03 Koninklijke Philips Electronics N. V. Biochip for fractionating and detecting analytes
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KR102403179B1 (en) * 2018-08-30 2022-05-26 주식회사 엘지화학 A disc type device incluing microbeads capable of controlling pH of a sample
WO2020046027A1 (en) * 2018-08-30 2020-03-05 주식회사 엘지화학 Device comprising microbeads capable of adjusting ph of sample
KR102431463B1 (en) * 2018-08-30 2022-08-12 주식회사 엘지화학 A device incluing microbeads capable of controlling pH of a sample
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Also Published As

Publication number Publication date
US20110210000A1 (en) 2011-09-01
US8076163B2 (en) 2011-12-13
WO2006087603A2 (en) 2006-08-24
GB0624182D0 (en) 2007-01-10
US7407816B2 (en) 2008-08-05
HRP20060424A2 (en) 2009-08-31
US20050250220A1 (en) 2005-11-10

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