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Publication numberWO2006064739 A1
Publication typeApplication
Application numberPCT/JP2005/022676
Publication date22 Jun 2006
Filing date9 Dec 2005
Priority date13 Dec 2004
Publication numberPCT/2005/22676, PCT/JP/2005/022676, PCT/JP/2005/22676, PCT/JP/5/022676, PCT/JP/5/22676, PCT/JP2005/022676, PCT/JP2005/22676, PCT/JP2005022676, PCT/JP200522676, PCT/JP5/022676, PCT/JP5/22676, PCT/JP5022676, PCT/JP522676, WO 2006/064739 A1, WO 2006064739 A1, WO 2006064739A1, WO-A1-2006064739, WO2006/064739A1, WO2006064739 A1, WO2006064739A1
InventorsTakeshi Nagasaka, Nagahide Matsubara, Noriaki Tanaka
ApplicantNational University Corporation Okayama University
Export CitationBiBTeX, EndNote, RefMan
External Links: Patentscope, Espacenet
Method of treating biological sample for performing nucleic acid amplification
WO 2006064739 A1
Abstract
It is intended to establish and provide a treatment method for conveniently extracting a nucleic acid in amplifying a target gene-origin nucleic acid likely contained in a biological sample without separating and purifying a nucleic acid such as a DNA from the sample by using complicated procedures as in the existing methods. Namely, a method which comprises: (a) the step of dissolving a biological sample in a buffer solution for dissolving the sample; (b) the step of impregnating a development support with the resultant solution; and (c) the step of bringing the development support into contact with a nucleic acid amplification solution. According to this method, a target gene-origin nucleic acid in a biological sample can be conveniently and efficiently amplified without resorting to complicated procedures for separating and purifying the nucleic acid.
Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
JP2001221721A * Title not available
JP2002306175A * Title not available
JPH08504081A * Title not available
US4483920 *17 May 198220 Nov 1984Hahnemann UniversityImmobilization of message RNA directly from cells onto filter material
Classifications
International ClassificationG01N33/53, C12N15/09, C12Q1/68
Cooperative ClassificationC12Q1/6806
European ClassificationC12Q1/68A4
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