WO2006062745A3 - Method for isolation of independent, parallel chemical micro-reactions using a porous filter - Google Patents

Method for isolation of independent, parallel chemical micro-reactions using a porous filter Download PDF

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Publication number
WO2006062745A3
WO2006062745A3 PCT/US2005/042522 US2005042522W WO2006062745A3 WO 2006062745 A3 WO2006062745 A3 WO 2006062745A3 US 2005042522 W US2005042522 W US 2005042522W WO 2006062745 A3 WO2006062745 A3 WO 2006062745A3
Authority
WO
WIPO (PCT)
Prior art keywords
independent
reactions
isolation
porous filter
parallel chemical
Prior art date
Application number
PCT/US2005/042522
Other languages
French (fr)
Other versions
WO2006062745A2 (en
Inventor
Said Attiya
Vinod B Makahijani
Ming Lei
Yi-Ju Chen
John Simpson
G Thomas Roth
Chun Heen Ho
Yu Pengguang
Original Assignee
454 Life Sciences Corp
Said Attiya
Vinod B Makahijani
Ming Lei
Yi-Ju Chen
John Simpson
G Thomas Roth
Chun Heen Ho
Yu Pengguang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2004/039208 external-priority patent/WO2005054431A2/en
Priority claimed from US11/217,194 external-priority patent/US20060088857A1/en
Application filed by 454 Life Sciences Corp, Said Attiya, Vinod B Makahijani, Ming Lei, Yi-Ju Chen, John Simpson, G Thomas Roth, Chun Heen Ho, Yu Pengguang filed Critical 454 Life Sciences Corp
Publication of WO2006062745A2 publication Critical patent/WO2006062745A2/en
Publication of WO2006062745A3 publication Critical patent/WO2006062745A3/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00353—Pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00414—Means for dispensing and evacuation of reagents using suction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00423—Means for dispensing and evacuation of reagents using filtration, e.g. through porous frits
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B01J2219/00466—Beads in a slurry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B01J2219/00468—Beads by manipulation of individual beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00704—Processes involving means for analysing and characterising the products integrated with the reactor apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

The present invention relates to methods and apparatuses for conducing densely packed, independent chemical reactions in parallel in fluid-permeable arrays. Accordingly, this invention also focuses on the use of such arrays for applications such as DNA sequencing, most preferably pyrophosphate sequencing, and DNA amplification.
PCT/US2005/042522 2004-11-23 2005-11-22 Method for isolation of independent, parallel chemical micro-reactions using a porous filter WO2006062745A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US11/016,942 2004-11-23
PCT/US2004/039208 WO2005054431A2 (en) 2003-12-01 2004-11-23 Method for isolation of independent, parallel chemical micro-reactions using a porous filter
US11/016,942 US20060019264A1 (en) 2003-12-01 2004-11-23 Method for isolation of independent, parallel chemical micro-reactions using a porous filter
USPCT/US2004/39208 2004-11-23
US11/217,194 2005-09-01
US11/217,194 US20060088857A1 (en) 2003-12-01 2005-09-01 Method for isolation of independent, parallel chemical micro-reactions using a porous filter

Publications (2)

Publication Number Publication Date
WO2006062745A2 WO2006062745A2 (en) 2006-06-15
WO2006062745A3 true WO2006062745A3 (en) 2007-07-26

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ID=36578389

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Application Number Title Priority Date Filing Date
PCT/US2005/042522 WO2006062745A2 (en) 2004-11-23 2005-11-22 Method for isolation of independent, parallel chemical micro-reactions using a porous filter

Country Status (1)

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009039378A2 (en) 2007-09-19 2009-03-26 The Charles Stark Draper Laboratory, Inc. Microfluidic structures for biomedical applications
CA2832494C (en) * 2011-04-06 2019-11-26 Ortho-Clinical Diagnostics, Inc. Assay device having rhombus-shaped projections
US20150191772A1 (en) * 2012-06-29 2015-07-09 Danmarks Tekniske Universitet Method of charging a test carrier and a test carrier

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2237696A (en) * 1939-08-18 1941-04-08 Charles H Biederman Circular window structure
US4853258A (en) * 1986-06-30 1989-08-01 Thomson-Csf Method for the impervious metallic coating of an optic fiber and device
US5610393A (en) * 1995-07-24 1997-03-11 The Aerospace Corporation Diode laser interrogated fiber optic reversible hydrazine-fuel sensor system and method
US5843767A (en) * 1993-10-28 1998-12-01 Houston Advanced Research Center Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions
US5858193A (en) * 1995-06-06 1999-01-12 Sarnoff Corporation Electrokinetic pumping
US5905262A (en) * 1996-02-27 1999-05-18 Spanswick; Keith Albert Radiation measuring apparatus
WO2000039587A1 (en) * 1998-12-28 2000-07-06 Illumina, Inc. Composite arrays utilizing microspheres
US6115528A (en) * 1998-09-21 2000-09-05 The United States Of America As Represented By The United States Department Of Energy Raman fiber optic probe assembly for use in hostile environments
US6184029B1 (en) * 1992-05-01 2001-02-06 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US6252034B1 (en) * 1998-06-04 2001-06-26 Teljin Limited Process for producing a polycarbonate resin
US6472163B1 (en) * 1998-04-20 2002-10-29 Kairos Scientific, Inc. Solid phase enzyme kinetics screening in microcolonies
US20030096268A1 (en) * 2001-07-06 2003-05-22 Michael Weiner Method for isolation of independent, parallel chemical micro-reactions using a porous filter

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2237696A (en) * 1939-08-18 1941-04-08 Charles H Biederman Circular window structure
US4853258A (en) * 1986-06-30 1989-08-01 Thomson-Csf Method for the impervious metallic coating of an optic fiber and device
US6184029B1 (en) * 1992-05-01 2001-02-06 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5843767A (en) * 1993-10-28 1998-12-01 Houston Advanced Research Center Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions
US5858193A (en) * 1995-06-06 1999-01-12 Sarnoff Corporation Electrokinetic pumping
US5610393A (en) * 1995-07-24 1997-03-11 The Aerospace Corporation Diode laser interrogated fiber optic reversible hydrazine-fuel sensor system and method
US5905262A (en) * 1996-02-27 1999-05-18 Spanswick; Keith Albert Radiation measuring apparatus
US6472163B1 (en) * 1998-04-20 2002-10-29 Kairos Scientific, Inc. Solid phase enzyme kinetics screening in microcolonies
US6252034B1 (en) * 1998-06-04 2001-06-26 Teljin Limited Process for producing a polycarbonate resin
US6115528A (en) * 1998-09-21 2000-09-05 The United States Of America As Represented By The United States Department Of Energy Raman fiber optic probe assembly for use in hostile environments
WO2000039587A1 (en) * 1998-12-28 2000-07-06 Illumina, Inc. Composite arrays utilizing microspheres
US20030096268A1 (en) * 2001-07-06 2003-05-22 Michael Weiner Method for isolation of independent, parallel chemical micro-reactions using a porous filter

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