WO2004109360A3 - Scanning microscope with a device for fluorescence correlation spectroscopy - Google Patents

Scanning microscope with a device for fluorescence correlation spectroscopy Download PDF

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Publication number
WO2004109360A3
WO2004109360A3 PCT/EP2004/050984 EP2004050984W WO2004109360A3 WO 2004109360 A3 WO2004109360 A3 WO 2004109360A3 EP 2004050984 W EP2004050984 W EP 2004050984W WO 2004109360 A3 WO2004109360 A3 WO 2004109360A3
Authority
WO
WIPO (PCT)
Prior art keywords
scanning microscope
correlation spectroscopy
fluorescence correlation
fluorescence
intensity
Prior art date
Application number
PCT/EP2004/050984
Other languages
German (de)
French (fr)
Other versions
WO2004109360A2 (en
Inventor
Joerg Bewersdorf
Original Assignee
Leica Microsystems
Joerg Bewersdorf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10329756A external-priority patent/DE10329756B4/en
Application filed by Leica Microsystems, Joerg Bewersdorf filed Critical Leica Microsystems
Priority to JP2006508311A priority Critical patent/JP2006526798A/en
Publication of WO2004109360A2 publication Critical patent/WO2004109360A2/en
Publication of WO2004109360A3 publication Critical patent/WO2004109360A3/en

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders

Abstract

The invention relates to a scanning microscope with a device for fluorescence correlation spectroscopy. The scanning microscope is characterized in that the intensity of the illumination light and/or the detection sensitivity is spatially modulated within the measuring volume.
PCT/EP2004/050984 2003-06-04 2004-06-02 Scanning microscope with a device for fluorescence correlation spectroscopy WO2004109360A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2006508311A JP2006526798A (en) 2003-06-04 2004-06-02 Scanning microscope with apparatus for fluorescence correlation spectroscopy

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10325506 2003-06-04
DE10325506.0 2003-06-04
DE10329756A DE10329756B4 (en) 2003-06-04 2003-07-02 Scanning microscope with a device for fluorescence correlation spectroscopy
DE10329756.1 2003-07-02

Publications (2)

Publication Number Publication Date
WO2004109360A2 WO2004109360A2 (en) 2004-12-16
WO2004109360A3 true WO2004109360A3 (en) 2005-02-10

Family

ID=33512382

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/050984 WO2004109360A2 (en) 2003-06-04 2004-06-02 Scanning microscope with a device for fluorescence correlation spectroscopy

Country Status (2)

Country Link
JP (1) JP2006526798A (en)
WO (1) WO2004109360A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0491289A1 (en) * 1990-12-18 1992-06-24 Stefan Dr. Hell Double-confocal scanning microscope
US6262423B1 (en) * 1996-12-22 2001-07-17 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E. V. Scanning microscope in which a sample is simultaneously and optically excited at various points

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0491289A1 (en) * 1990-12-18 1992-06-24 Stefan Dr. Hell Double-confocal scanning microscope
US6262423B1 (en) * 1996-12-22 2001-07-17 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E. V. Scanning microscope in which a sample is simultaneously and optically excited at various points

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DIASPRO A ET AL: "TWO-PHOTON MICROSCOPY AND SPECTROSCOPY BASED ON A COMPACT CONFOCAL SCANNING HEAD", JOURNAL OF BIOMEDICAL OPTICS, vol. 6, no. 3, July 2001 (2001-07-01), pages 300 - 310, XP002306388 *
SCHRADER M ET AL: "OPTICAL TRANSFER FUNCTIONS OF 4PI CONFOCAL MICROSCOPES: THEORY AND EXPERIMENT", OPTICS LETTERS, vol. 22, no. 7, 1 April 1997 (1997-04-01), USA, pages 436 - 438, XP002306389 *
SOINI J T ET AL: "IMAGE FORMATION AND DATA ACQUISITION IN A STAGE SCANNING API CONFOCAL FLUORESCENCE MICROSCOPE", APPLIED OPTICS, OPTICAL SOCIETY OF AMERICA,WASHINGTON, US, vol. 36, no. 34, 1 December 1997 (1997-12-01), pages 8929 - 8934, XP000728467, ISSN: 0003-6935 *
WINFRIED WIEGRÄBE: "FLUORESCENCE CORRELATION SPECTROSCOPY: PROBING MOLECULAR INTERACTIONS INSIDE LIVING CELLS", AMERICAN LABORATORY, September 2000 (2000-09-01), pages 44 - 47, XP002306387 *

Also Published As

Publication number Publication date
WO2004109360A2 (en) 2004-12-16
JP2006526798A (en) 2006-11-24

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