WO2004058989A3 - Target-dependent transcription - Google Patents

Target-dependent transcription Download PDF

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Publication number
WO2004058989A3
WO2004058989A3 PCT/US2003/040912 US0340912W WO2004058989A3 WO 2004058989 A3 WO2004058989 A3 WO 2004058989A3 US 0340912 W US0340912 W US 0340912W WO 2004058989 A3 WO2004058989 A3 WO 2004058989A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
transcription
kits
compositions
nucleic acid
Prior art date
Application number
PCT/US2003/040912
Other languages
French (fr)
Other versions
WO2004058989A2 (en
Original Assignee
Epict Technologies
Dahl Gary A
Jendrisak Jerome J
Meis Judy E
Vaidyanathan Ramesh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epict Technologies, Dahl Gary A, Jendrisak Jerome J, Meis Judy E, Vaidyanathan Ramesh filed Critical Epict Technologies
Priority to AU2003301219A priority Critical patent/AU2003301219A1/en
Priority to EP03814301A priority patent/EP1583840A4/en
Publication of WO2004058989A2 publication Critical patent/WO2004058989A2/en
Publication of WO2004058989A3 publication Critical patent/WO2004058989A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

The present invention comprises novel methods, compositions and kits comprising monopartite or bipartite target probes and an RNA polymerase to detect and quantify analytes comprising one or multiple target nucleic acid sequences, including target sequences that differ by as little as one nucleotide, or to detect and quantify non-nucleic acid analytes by detecting a target sequence tag that is joined to an analyte-binding substance. The method, called 'target­dependent transcription,' consists of an annealing process, a DNA ligation process, a transcription process, and a detection process. The invention also comprises novel methods, compositions and kits for amplifying RNA, including strand-displacement reverse transcription and rolling circle reverse transcription.
PCT/US2003/040912 2002-12-23 2003-12-23 Target-dependent transcription WO2004058989A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003301219A AU2003301219A1 (en) 2002-12-23 2003-12-23 Target-dependent transcription
EP03814301A EP1583840A4 (en) 2002-12-23 2003-12-23 Target-dependent transcription

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43606202P 2002-12-23 2002-12-23
US60/436,062 2002-12-23

Publications (2)

Publication Number Publication Date
WO2004058989A2 WO2004058989A2 (en) 2004-07-15
WO2004058989A3 true WO2004058989A3 (en) 2007-10-18

Family

ID=32682331

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/040912 WO2004058989A2 (en) 2002-12-23 2003-12-23 Target-dependent transcription

Country Status (3)

Country Link
EP (1) EP1583840A4 (en)
AU (1) AU2003301219A1 (en)
WO (1) WO2004058989A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101248191A (en) * 2005-07-06 2008-08-20 生物芯片创新控股有限公司 Method and kit for analyzing a target nucleic acid sequence
CA2848304A1 (en) 2011-09-09 2013-03-14 The Board Of Trustees Of The Leland Stanford Junior University Methods for sequencing a polynucleotide
CN106801050B (en) * 2017-02-20 2020-01-14 广州永诺生物科技有限公司 Construction method of circular RNA high-throughput sequencing library and kit thereof
GB201904081D0 (en) * 2019-03-25 2019-05-08 Feyrer Hannes Method and products for producing RNA molecules

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5683888A (en) * 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5700667A (en) * 1993-09-20 1997-12-23 Regents Of The University Of Colorado, The Strategy for the production of RNA from immobilized templates
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6593086B2 (en) * 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5683888A (en) * 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5700667A (en) * 1993-09-20 1997-12-23 Regents Of The University Of Colorado, The Strategy for the production of RNA from immobilized templates
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of EP1583840A4 *
TYAGI S. ET AL.: "Extremely sensitive, background-free gene detection using binary probes and Qb replicase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 93, no. 11, May 1996 (1996-05-01), pages 5395 - 5400, XP000600188 *

Also Published As

Publication number Publication date
EP1583840A4 (en) 2009-03-18
EP1583840A2 (en) 2005-10-12
AU2003301219A8 (en) 2004-07-22
AU2003301219A1 (en) 2004-07-22
WO2004058989A2 (en) 2004-07-15

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