WO2004055492A3 - Closed-loop control of electrokinetic processes in microfludic devices based on optical readings - Google Patents

Closed-loop control of electrokinetic processes in microfludic devices based on optical readings Download PDF

Info

Publication number
WO2004055492A3
WO2004055492A3 PCT/US2003/039135 US0339135W WO2004055492A3 WO 2004055492 A3 WO2004055492 A3 WO 2004055492A3 US 0339135 W US0339135 W US 0339135W WO 2004055492 A3 WO2004055492 A3 WO 2004055492A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
closed
loop control
electric field
devices based
Prior art date
Application number
PCT/US2003/039135
Other languages
French (fr)
Other versions
WO2004055492A2 (en
Inventor
Ann Wainright
Torleif Bjornson
Original Assignee
Aclara Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc filed Critical Aclara Biosciences Inc
Priority to AU2003294602A priority Critical patent/AU2003294602A1/en
Publication of WO2004055492A2 publication Critical patent/WO2004055492A2/en
Publication of WO2004055492A3 publication Critical patent/WO2004055492A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Abstract

A method for performing an electrophoretic separation on a microfludic device includes applying an electric field to electrokinetically move a sample along a channel towards a location. The sample may be concentrated by application of the electric field. The method further comprises optically monitoring the location for a least a portion of the sample. Once the sample is detected, the electric field is automatically changed to further manipulate the sample. The sample may be spatially separated along a separation channel or channel portion. The closed-loop control of electric fields may be performed using a control unit adapted to apply a voltage potential between electrodes and an optical detector sucha as an LIF detector.
PCT/US2003/039135 2002-12-13 2003-12-10 Closed-loop control of electrokinetic processes in microfludic devices based on optical readings WO2004055492A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003294602A AU2003294602A1 (en) 2002-12-13 2003-12-10 Closed-loop control of electrokinetic processes in microfludic devices based on optical readings

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43318302P 2002-12-13 2002-12-13
US60/433,183 2002-12-13

Publications (2)

Publication Number Publication Date
WO2004055492A2 WO2004055492A2 (en) 2004-07-01
WO2004055492A3 true WO2004055492A3 (en) 2004-12-09

Family

ID=32595131

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/039135 WO2004055492A2 (en) 2002-12-13 2003-12-10 Closed-loop control of electrokinetic processes in microfludic devices based on optical readings

Country Status (3)

Country Link
US (1) US6905583B2 (en)
AU (1) AU2003294602A1 (en)
WO (1) WO2004055492A2 (en)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7655477B1 (en) 2003-02-26 2010-02-02 Science Applications International Corporation System and method for the separation of analytes
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7731906B2 (en) 2003-07-31 2010-06-08 Handylab, Inc. Processing particle-containing samples
AU2005241080B2 (en) 2004-05-03 2011-08-11 Handylab, Inc. Processing polynucleotide-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
JP2006317357A (en) * 2005-05-13 2006-11-24 Shimadzu Corp Microchip electrophoretic method and microchip electrophoretic apparatus
WO2007047606A2 (en) * 2005-10-17 2007-04-26 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Electrokinetic thermal cycler and reactor
EP1957794B1 (en) 2005-11-23 2014-07-02 Eksigent Technologies, LLC Electrokinetic pump designs and drug delivery systems
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP3088083B1 (en) 2006-03-24 2018-08-01 Handylab, Inc. Method of performing pcr with a mult-ilane cartridge
US8414754B1 (en) 2006-05-31 2013-04-09 The Board Of Trustees Of The Leland Stanford Junior University Electrophoretic sample analysis and approach therefor
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
CA2580589C (en) * 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
WO2008119184A1 (en) 2007-04-02 2008-10-09 Fio Corporation System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology
WO2009000084A1 (en) 2007-06-22 2008-12-31 Fio Corporation Systems and methods for manufacturing quantum dot-doped polymer microbeads
WO2009006739A1 (en) * 2007-07-09 2009-01-15 Fio Corporation Systems and methods for enhancing fluorescent detection of target molecules in a test sample
CA2693654C (en) 2007-07-13 2018-02-13 Handylab, Inc. Polynucleotide capture materials, and methods of using same
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
KR101832658B1 (en) * 2007-07-23 2018-02-26 피오 코포레이션 A method and system for collating, storing, analyzing and enabling access to collected and analyzed data associated with biological and environmental test subjects
CN101861203B (en) 2007-10-12 2014-01-22 Fio公司 Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto
CN102132330B (en) 2008-06-25 2015-07-22 Fio公司 Bio-threat alert system
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
CN102165321B (en) 2008-08-29 2016-03-16 Fio公司 For detecting the disposable hand-held diagnostic detection device of biological and environment measuring sample and related system and method
CN102348986B (en) 2009-01-13 2015-05-06 Fio公司 A handheld diagnostic test device and method for use with an electronic device and a test cartridge in a rapid diagnostic test
CN106148512B (en) 2011-04-15 2020-07-10 贝克顿·迪金森公司 Scanning real-time microfluidic thermocycler and method for synchronized thermocycling and scanning optical detection
CN103813814A (en) 2011-05-05 2014-05-21 艾克西根特技术有限公司 Gel coupling for electrokinetic delivery system
DK2761305T3 (en) 2011-09-30 2017-11-20 Becton Dickinson Co United Reagent Strip
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
CN104204812B (en) 2012-02-03 2018-01-05 贝克顿·迪金森公司 The external file that compatibility determines between distributing and test for molecule diagnostic test
CN102886280B (en) * 2012-08-28 2014-06-11 博奥生物有限公司 Microfluidic chip and application thereof
CN107615031B (en) * 2015-03-24 2020-09-01 新加坡国立大学 Resistance type microfluid pressure sensor
EP3916090A1 (en) 2016-01-29 2021-12-01 Purigen Biosystems, Inc. Isotachophoresis for purification of nucleic acids
EP3500847A4 (en) * 2016-08-18 2020-04-15 Shenzhen Genorivision Technology Co., Ltd. An electrophoresis chip with an integrated optical sensor
JP7203106B2 (en) 2017-08-02 2023-01-12 ピュリゲン バイオシステムズ インコーポレイテッド Systems, apparatus and methods for isotachophoresis
CN109030609A (en) * 2018-07-06 2018-12-18 中国科学院合肥物质科学研究院 A kind of minor effect genes system and its detection method synchronizing detection zwitterion

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2378334A (en) * 1943-09-29 1945-06-12 Sirvent Philip Botaya Comparator gauge
WO1992013229A2 (en) * 1991-01-08 1992-08-06 Europhor S.A. Analysis method by capillary electrophoresis with fluorescence detection
EP1162455A1 (en) * 1994-08-01 2001-12-12 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipultions for chemical analysis and synthesis
US20020079223A1 (en) * 2000-02-11 2002-06-27 Williams Stephen J. Tandem isotachophoresis/zone electrophoresis method and system
US20020189946A1 (en) * 2000-02-11 2002-12-19 Aclara Biosciences, Inc. Microfluidic injection and separation system and method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5378334A (en) * 1988-08-24 1995-01-03 The Board Of Trustees Of The Leland Stanford Junior University System for measuring and controlling electroosmosis in separation techniques
EP0653631B1 (en) * 1993-11-11 2003-05-14 Aclara BioSciences, Inc. Apparatus and method for the electrophoretical separation of mixtures of fluid substances
US6391622B1 (en) * 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
ATE445155T1 (en) * 2000-02-11 2009-10-15 Aclara Biosciences Inc MICROFLUIDIC DEVICE HAVING A LIQUID SAMPLE INJECTION DEVICE AND METHOD OF USE

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2378334A (en) * 1943-09-29 1945-06-12 Sirvent Philip Botaya Comparator gauge
WO1992013229A2 (en) * 1991-01-08 1992-08-06 Europhor S.A. Analysis method by capillary electrophoresis with fluorescence detection
EP1162455A1 (en) * 1994-08-01 2001-12-12 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipultions for chemical analysis and synthesis
US20020079223A1 (en) * 2000-02-11 2002-06-27 Williams Stephen J. Tandem isotachophoresis/zone electrophoresis method and system
US20020189946A1 (en) * 2000-02-11 2002-12-19 Aclara Biosciences, Inc. Microfluidic injection and separation system and method

Also Published As

Publication number Publication date
US6905583B2 (en) 2005-06-14
WO2004055492A2 (en) 2004-07-01
AU2003294602A8 (en) 2004-07-09
AU2003294602A1 (en) 2004-07-09
US20040118684A1 (en) 2004-06-24

Similar Documents

Publication Publication Date Title
WO2004055492A3 (en) Closed-loop control of electrokinetic processes in microfludic devices based on optical readings
TW345683B (en) Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
WO2010028140A3 (en) Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
EP1486298A4 (en) Robot device, legged locomotion robot operation control device and operation control method, legged locomotion robot sensor system, and locomotion device
AU4773400A (en) Methods and apparatus for nonlinear mobility electrophoresis separation
WO2004090633A3 (en) An electro-osmotic element for an immersion lithography apparatus
DE60328131D1 (en) VARACTORY DEVICES AND METHOD
JP2008529677A5 (en)
AU2003256310A1 (en) An apparatus and method for point cloud assembly
DE60104636D1 (en) System for information about methods for setting control parameters of an electric power steering control device
WO2004031353A3 (en) Apparatus and method for streaming electroporation
JP2016508616A5 (en)
CA2176317A1 (en) Fast sampling device and sampling method for capillary electrophoresis
EP3731190A4 (en) Human-machine interaction method and apparatus based on high-precision map
ATE282201T1 (en) CONDUCTIVITY MEASUREMENT WITH A SINGLE ELECTRODE
WO2005054263A3 (en) Analyte identification in transformed electropherograms
ATE528643T1 (en) ELECTROPHORESIS METHOD AND DEVICE FOR SEPARATING OBJECTS
WO2004012231A3 (en) Method and apparatus for control of mobility-based ion species identification
FI20020924A (en) Method and apparatus for controlling the separation of liquid-liquid extraction dispersion
AU4128500A (en) Electrophoresis method and apparatus with time- or space-modulated sample injection
DK1274511T3 (en) Method and apparatus for microdosing the smallest amounts of liquid for biopolymer arrays
WO2008090925A1 (en) Control liquid judging method and analysis device
DE59905566D1 (en) DEVICE AND METHOD FOR THE MINIATURIZED, HIGH-PARALLEL ELECTROPHORETIC SEPARATION
WO2009152198A3 (en) Analytical instrument
WO2007046484A8 (en) Electrophoretic chip, electrophoretic device, and electrophoretic system

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP