WO2004042403A3 - Methods, device and instrument for detection of analytes - Google Patents

Methods, device and instrument for detection of analytes Download PDF

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Publication number
WO2004042403A3
WO2004042403A3 PCT/EP2003/012234 EP0312234W WO2004042403A3 WO 2004042403 A3 WO2004042403 A3 WO 2004042403A3 EP 0312234 W EP0312234 W EP 0312234W WO 2004042403 A3 WO2004042403 A3 WO 2004042403A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
particle structure
sample
particle
instrument
Prior art date
Application number
PCT/EP2003/012234
Other languages
French (fr)
Other versions
WO2004042403A2 (en
Inventor
Jochen Feldmann
Thomas Franzl
Thomas Klar
Gunnar Raschke
Carsten Soennichsen
Stefan Kowarik
Alfons Nichtl
Konrad Kuerzinger
Original Assignee
Ludwig Maximilian Uni Versitae
Roche Diagnostics Gmbh
Jochen Feldmann
Thomas Franzl
Thomas Klar
Gunnar Raschke
Carsten Soennichsen
Stefan Kowarik
Alfons Nichtl
Konrad Kuerzinger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ludwig Maximilian Uni Versitae, Roche Diagnostics Gmbh, Jochen Feldmann, Thomas Franzl, Thomas Klar, Gunnar Raschke, Carsten Soennichsen, Stefan Kowarik, Alfons Nichtl, Konrad Kuerzinger filed Critical Ludwig Maximilian Uni Versitae
Priority to AU2003289853A priority Critical patent/AU2003289853A1/en
Publication of WO2004042403A2 publication Critical patent/WO2004042403A2/en
Publication of WO2004042403A3 publication Critical patent/WO2004042403A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons
    • G01N21/554—Attenuated total reflection and using surface plasmons detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle

Abstract

The present invention relates to a method, a device and an instrument for the detection of an analyte (4) in a sample(6). Such method, device and instrument is used in clinical diagnostics, pharmacology, e.g. drug screening, environmental analysis as well as in other fields of analysis like chemistry or biochemistry. According to the present invention, the method for detection of an analyte (4) in a sample comprises the following steps of a) providing at least one particle structure (1) detectable by light scattering and coated with binding entities (2) for said analyte (4); b) contacting the at least one particle structure (1) with the sample (6), c) illuminating the at least one particle structure (1) and measuring the scattered light emitted by individual particle structures (1) at least twice and d) determining a change in the spectral signature of the scattered light as a measure of the presence and/or quantity of the analyte (4) in the sample (6). In order to detect more than one analyte (4) simultaneously particle structures (1) of different classes of particle structures maybe coated with different binding entities. Further, instead of coatig the particle structure (1) with binding entities specific for the analyte (4), the particle structure (1) may be coated with substances (21) competing with said analyte (4) for binding to specific binding entities (2). Any competitive and non-competitive assay format can be put into practice.
PCT/EP2003/012234 2002-11-04 2003-11-03 Methods, device and instrument for detection of analytes WO2004042403A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003289853A AU2003289853A1 (en) 2002-11-04 2003-11-03 Methods, device and instrument for detection of analytes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP02024379 2002-11-04
EP02024379.6 2002-11-04
EP03005005 2003-03-05
EP03005005.8 2003-03-05

Publications (2)

Publication Number Publication Date
WO2004042403A2 WO2004042403A2 (en) 2004-05-21
WO2004042403A3 true WO2004042403A3 (en) 2004-07-29

Family

ID=32313827

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/012234 WO2004042403A2 (en) 2002-11-04 2003-11-03 Methods, device and instrument for detection of analytes

Country Status (2)

Country Link
AU (1) AU2003289853A1 (en)
WO (1) WO2004042403A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
JP7208226B2 (en) 2017-09-22 2023-01-18 ノキア テクノロジーズ オーユー functional particles

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ528323A (en) * 2003-09-18 2006-05-26 Horticulture & Food Res Inst Immunoassay
US20070134815A1 (en) * 2005-07-11 2007-06-14 Danielle Chamberlin Sensitivity enhancement of POCT devices using gold and silver nanoparticles on substrates containing nanostructures or nanoparticles that interact with labeling particles
US8865454B2 (en) 2007-03-22 2014-10-21 Scandinavian Micro Biodevices Aps Flow through system, flow through device and a method of performing a test
WO2011148870A1 (en) * 2010-05-28 2011-12-01 国立大学法人東京工業大学 Metal microparticle composite and method for producing the same
US9772305B2 (en) 2011-09-15 2017-09-26 Arizona Board Of Regents On Behalf Of Arizona State University System and method for small molecule detection
EP2587252A1 (en) * 2011-10-26 2013-05-01 Carsten Sönnichsen Individual molecule detection by plasmonic nanoparticles
CN102565395B (en) * 2012-02-14 2014-04-16 北京大学 Method for detecting bacteria amount of gold nanoparticles by using coated antibody
WO2014192937A1 (en) 2013-05-30 2014-12-04 公立大学法人大阪府立大学 Target-substance detection apparatus and method
US20150369801A1 (en) * 2014-06-20 2015-12-24 Carsten Sönnichsen Method for Detecting and/or Quantifying the Binding Affinities of a Target Molecule to a Plurality of Different Binding Partners by Plasmon Resonance of Nanoparticles and a Position-Encoded Sensor Therefor
JPWO2016039149A1 (en) * 2014-09-10 2017-06-22 コニカミノルタ株式会社 Detection method and detection apparatus
WO2016170183A1 (en) 2015-04-24 2016-10-27 Pharmadiagnostics Nv Improved method of measuring an interaction between molecules
US11557042B2 (en) * 2018-06-12 2023-01-17 King Abdullah University Of Science And Technology Single-camera particle tracking system and method
DE102020216450A1 (en) * 2020-12-22 2022-06-23 CMO-SYS GmbH Device and method for detecting at least one substance

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4446239A (en) * 1981-04-24 1984-05-01 Chugai Seiyaku Kabushiki Kaisha Light scattering immunoassay involving particles with selective frequency band apparatus
US6180415B1 (en) * 1997-02-20 2001-01-30 The Regents Of The University Of California Plasmon resonant particles, methods and apparatus
US6214560B1 (en) * 1996-04-25 2001-04-10 Genicon Sciences Corporation Analyte assay using particulate labels

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4446239A (en) * 1981-04-24 1984-05-01 Chugai Seiyaku Kabushiki Kaisha Light scattering immunoassay involving particles with selective frequency band apparatus
US6214560B1 (en) * 1996-04-25 2001-04-10 Genicon Sciences Corporation Analyte assay using particulate labels
US6180415B1 (en) * 1997-02-20 2001-01-30 The Regents Of The University Of California Plasmon resonant particles, methods and apparatus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HAES A J ET AL: "A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 124, no. 35, 2002, pages 10596 - 10604, XP002237025, ISSN: 0002-7863 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
JP7208226B2 (en) 2017-09-22 2023-01-18 ノキア テクノロジーズ オーユー functional particles

Also Published As

Publication number Publication date
AU2003289853A1 (en) 2004-06-07
WO2004042403A2 (en) 2004-05-21
AU2003289853A8 (en) 2004-06-07

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