WO2004034027A3 - Method for sizing polynucleotides using electrophoresis with non-dna size standards - Google Patents

Method for sizing polynucleotides using electrophoresis with non-dna size standards Download PDF

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Publication number
WO2004034027A3
WO2004034027A3 PCT/US2003/032146 US0332146W WO2004034027A3 WO 2004034027 A3 WO2004034027 A3 WO 2004034027A3 US 0332146 W US0332146 W US 0332146W WO 2004034027 A3 WO2004034027 A3 WO 2004034027A3
Authority
WO
WIPO (PCT)
Prior art keywords
size standards
sample polynucleotide
electrophoresis
size
polynucleotides
Prior art date
Application number
PCT/US2003/032146
Other languages
French (fr)
Other versions
WO2004034027A2 (en
Inventor
Zhaowei Liu
Thomas Kane
Original Assignee
Spectrumedix Llc
Zhaowei Liu
Thomas Kane
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spectrumedix Llc, Zhaowei Liu, Thomas Kane filed Critical Spectrumedix Llc
Priority to AU2003282574A priority Critical patent/AU2003282574A1/en
Priority to EP03774761A priority patent/EP1549927A2/en
Priority to JP2004543653A priority patent/JP2006507485A/en
Publication of WO2004034027A2 publication Critical patent/WO2004034027A2/en
Publication of WO2004034027A3 publication Critical patent/WO2004034027A3/en
Priority to US11/103,356 priority patent/US7435545B2/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The present invention relates to a method of determining a size of a sample polynucleotide. A sample polynucleotide is subjected to electrophoresis in the presence of at least: a fluorescent compound having a first fluorescence spectrum, wherein detection of light of the first fluorescence spectrum is indicative of the presence of the sample polynucleotide, and a plurality of size standards, each of the size standards having a different mobility and wherein each of the size standards is essentially free of polynucleotides. Migration coordinates of the sample polynucleotide and plurality of size standards are determined. A size of the sample polynucleotide is determined using the migration coordinate of the sample polynucleotide and the migration coordinates of the size standards.
PCT/US2003/032146 2002-05-01 2003-10-10 Method for sizing polynucleotides using electrophoresis with non-dna size standards WO2004034027A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2003282574A AU2003282574A1 (en) 2002-10-10 2003-10-10 Method for sizing polynucleotides using electrophoresis with non-dna size standards
EP03774761A EP1549927A2 (en) 2002-10-10 2003-10-10 Method for sizing polynucleotides using electrophoresis with non-dna size standards
JP2004543653A JP2006507485A (en) 2002-10-10 2003-10-10 Method for determining the size of a polynucleotide using electrophoresis with a non-DNA size standard
US11/103,356 US7435545B2 (en) 2002-05-01 2005-04-11 Method for sizing polynucleotides using electrophoresis with non-DNA size standards

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41717302P 2002-10-10 2002-10-10
US60/417,173 2002-10-10

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US10/425,746 Continuation-In-Part US20030207310A1 (en) 2002-05-01 2003-04-30 System and method for determining the sizes and quantity of polynucleotides with capillary array electrophoresis

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/103,356 Continuation-In-Part US7435545B2 (en) 2002-05-01 2005-04-11 Method for sizing polynucleotides using electrophoresis with non-DNA size standards

Publications (2)

Publication Number Publication Date
WO2004034027A2 WO2004034027A2 (en) 2004-04-22
WO2004034027A3 true WO2004034027A3 (en) 2004-11-11

Family

ID=32093980

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/032146 WO2004034027A2 (en) 2002-05-01 2003-10-10 Method for sizing polynucleotides using electrophoresis with non-dna size standards

Country Status (4)

Country Link
EP (1) EP1549927A2 (en)
JP (1) JP2006507485A (en)
AU (1) AU2003282574A1 (en)
WO (1) WO2004034027A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7238486B2 (en) * 2004-09-21 2007-07-03 The Board Of Trustees Of The Leland Stanford Junior University DNA fingerprinting using a branch migration assay
US20230392188A1 (en) * 2022-06-02 2023-12-07 Perkinelmer Health Sciences, Inc. Electrophoresis-mediated characterization of dna content of adeno-associated virus capsids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5162514A (en) * 1990-05-15 1992-11-10 Board Of Regents, University Of Texas High molecular weight dna compositions for use in electrophoresis of large nucleic acids
US5302510A (en) * 1992-07-27 1994-04-12 Life Technologies, Inc. DNA sizing control standards for electrophoretic analyses
US5599664A (en) * 1989-04-05 1997-02-04 New York University Method for characterizing polymer molecules or the like

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5599664A (en) * 1989-04-05 1997-02-04 New York University Method for characterizing polymer molecules or the like
US5162514A (en) * 1990-05-15 1992-11-10 Board Of Regents, University Of Texas High molecular weight dna compositions for use in electrophoresis of large nucleic acids
US5302510A (en) * 1992-07-27 1994-04-12 Life Technologies, Inc. DNA sizing control standards for electrophoretic analyses

Also Published As

Publication number Publication date
EP1549927A2 (en) 2005-07-06
WO2004034027A2 (en) 2004-04-22
AU2003282574A8 (en) 2004-05-04
AU2003282574A1 (en) 2004-05-04
JP2006507485A (en) 2006-03-02

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