WO2004031399A3 - Parallel loading of arrays - Google Patents

Parallel loading of arrays Download PDF

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Publication number
WO2004031399A3
WO2004031399A3 PCT/US2003/030841 US0330841W WO2004031399A3 WO 2004031399 A3 WO2004031399 A3 WO 2004031399A3 US 0330841 W US0330841 W US 0330841W WO 2004031399 A3 WO2004031399 A3 WO 2004031399A3
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WO
WIPO (PCT)
Prior art keywords
arrays
parallel loading
microarray
loading
sub
Prior art date
Application number
PCT/US2003/030841
Other languages
French (fr)
Other versions
WO2004031399A2 (en
Inventor
Roland D Green
Mark Mccormick
Gary Barrett
Original Assignee
Nimblegen Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nimblegen Systems Inc filed Critical Nimblegen Systems Inc
Priority to AU2003282885A priority Critical patent/AU2003282885A1/en
Priority to CA2500936A priority patent/CA2500936C/en
Priority to DE10393406T priority patent/DE10393406T5/en
Priority to GB0508821A priority patent/GB2409455B/en
Priority to JP2005500338A priority patent/JP4471927B2/en
Publication of WO2004031399A2 publication Critical patent/WO2004031399A2/en
Publication of WO2004031399A3 publication Critical patent/WO2004031399A3/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
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    • C40—COMBINATORIAL TECHNOLOGY
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices
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    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B01J2219/00619—Delimitation of the attachment areas by chemical means using hydrophilic or hydrophobic regions
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
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    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00691—Automatic using robots
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    • B01J2219/00718—Type of compounds synthesised
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    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
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    • B01L2400/04—Moving fluids with specific forces or mechanical means
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    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Abstract

The present inventions provides an apparatus (20) and method for the parallel loading of multiple samples in a microarray (10) containing a plurality of sub-arrays (14). The method makes use of a microarray (10) containing multiple sub-arrays (14), a loading channel array (20), and a fluid handling robot or an assembly robot or machine.
PCT/US2003/030841 2002-09-30 2003-09-30 Parallel loading of arrays WO2004031399A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2003282885A AU2003282885A1 (en) 2002-09-30 2003-09-30 Parallel loading of arrays
CA2500936A CA2500936C (en) 2002-09-30 2003-09-30 Parallel loading of arrays
DE10393406T DE10393406T5 (en) 2002-09-30 2003-09-30 Parallel loading of arrays
GB0508821A GB2409455B (en) 2002-09-30 2003-09-30 Parallel loading of arrays
JP2005500338A JP4471927B2 (en) 2002-09-30 2003-09-30 Array parallel loading method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41511802P 2002-09-30 2002-09-30
US60/415,118 2002-09-30
US41509002P 2002-10-01 2002-10-01
US60/415,090 2002-10-01

Publications (2)

Publication Number Publication Date
WO2004031399A2 WO2004031399A2 (en) 2004-04-15
WO2004031399A3 true WO2004031399A3 (en) 2005-02-24

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/030841 WO2004031399A2 (en) 2002-09-30 2003-09-30 Parallel loading of arrays

Country Status (7)

Country Link
US (2) US7482170B2 (en)
JP (1) JP4471927B2 (en)
AU (1) AU2003282885A1 (en)
CA (1) CA2500936C (en)
DE (1) DE10393406T5 (en)
GB (1) GB2409455B (en)
WO (1) WO2004031399A2 (en)

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US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

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US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7498176B2 (en) * 2002-09-27 2009-03-03 Roche Nimblegen, Inc. Microarray with hydrophobic barriers
WO2005082098A2 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20070196834A1 (en) * 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
DE102008021364A1 (en) * 2008-04-29 2009-06-25 Siemens Aktiengesellschaft Charging dry reagents into analysis unit used e.g. in biotechnology and genetic engineering, applies dry reagents to transfer reservoir to be brought into contact with analysis unit
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US20100161607A1 (en) * 2008-12-22 2010-06-24 Jasjit Singh System and method for analyzing genome data
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
ES2574055T3 (en) 2009-11-25 2016-06-14 Gen9, Inc. Methods and apparatus for error reduction in chip-based DNA
EP2506973A2 (en) * 2009-12-02 2012-10-10 Roche Diagniostics GmbH Multiplexed microarray and method of fabricating thereof
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US10240194B2 (en) 2010-05-13 2019-03-26 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
WO2011150168A1 (en) 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
KR101251223B1 (en) * 2010-08-25 2013-04-08 서울대학교산학협력단 A Method for Manufacturing liquid Droplet Microarrays, Microarrays Prepared by Using the Same, a Device for Delivering Materials and a Method for Delivering Materials by Using a Device for Delivering Materials Comprising the Same
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
US8753873B2 (en) 2011-04-15 2014-06-17 Roche Nimblegen, Inc. Multiplexed microarray assembly and method for fabricating a multiplexed microarray
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
EP3517611A3 (en) 2011-06-15 2019-09-04 Gen9, Inc. Methods for preparative in vitro cloning
AU2012300401B2 (en) 2011-08-26 2018-02-08 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
WO2013163263A2 (en) 2012-04-24 2013-10-31 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
CN104685116A (en) 2012-06-25 2015-06-03 Gen9股份有限公司 Methods for nucleic acid assembly and high throughput sequencing
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