WO2004015416A3 - Methods for the identification of agents with anti-microbial action - Google Patents

Methods for the identification of agents with anti-microbial action Download PDF

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Publication number
WO2004015416A3
WO2004015416A3 PCT/EP2003/008145 EP0308145W WO2004015416A3 WO 2004015416 A3 WO2004015416 A3 WO 2004015416A3 EP 0308145 W EP0308145 W EP 0308145W WO 2004015416 A3 WO2004015416 A3 WO 2004015416A3
Authority
WO
WIPO (PCT)
Prior art keywords
agents
identification
methods
microbial action
eukaryotic cells
Prior art date
Application number
PCT/EP2003/008145
Other languages
German (de)
French (fr)
Other versions
WO2004015416A2 (en
Inventor
Gerald Kleymann
Hans-Otto Werling
Original Assignee
Bayer Healthcare Ag
Gerald Kleymann
Hans-Otto Werling
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Healthcare Ag, Gerald Kleymann, Hans-Otto Werling filed Critical Bayer Healthcare Ag
Priority to AU2003255282A priority Critical patent/AU2003255282A1/en
Publication of WO2004015416A2 publication Critical patent/WO2004015416A2/en
Publication of WO2004015416A3 publication Critical patent/WO2004015416A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a novel method and the use thereof for the discovery of novel anti-microbial agents, characterised in that potential agents are simultaneously incubated with eukaryotic cells and non-viral microbes in a test composition, the viability of the eukaryotic cells is determined and subsequently those agents are selected for which the signal for the corresponding test composition is significantly larger than the mean value of the infection control at at least one concentration of the agent.
PCT/EP2003/008145 2002-08-06 2003-07-24 Methods for the identification of agents with anti-microbial action WO2004015416A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003255282A AU2003255282A1 (en) 2002-08-06 2003-07-24 Methods for the identification of agents with anti-microbial action

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10235967.9 2002-08-06
DE2002135967 DE10235967B4 (en) 2002-08-06 2002-08-06 Method for identifying substances with antimicrobial activity

Publications (2)

Publication Number Publication Date
WO2004015416A2 WO2004015416A2 (en) 2004-02-19
WO2004015416A3 true WO2004015416A3 (en) 2004-05-06

Family

ID=30775043

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/008145 WO2004015416A2 (en) 2002-08-06 2003-07-24 Methods for the identification of agents with anti-microbial action

Country Status (3)

Country Link
AU (1) AU2003255282A1 (en)
DE (1) DE10235967B4 (en)
WO (1) WO2004015416A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0822260D0 (en) * 2008-12-05 2009-01-14 Merten Christoph Assay
AR108175A1 (en) 2016-04-06 2018-07-25 Innovative Molecules Gmbh TIAZOL ACETAMIDA ANTIVÍRICOS COMPOUNDS
CA3077397A1 (en) 2017-10-05 2019-04-11 Innovative Molecules Gmbh Enantiomers of substituted thiazoles as antiviral compounds

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5366738A (en) * 1982-07-29 1994-11-22 Merck & Co., Inc. Controlled release drug dispersion delivery device
US5369095A (en) * 1990-02-14 1994-11-29 Alcon Laboratories, Inc. Compositions and method comprising substituted glycosides as mucus membrane permeation enhancers
US5501959A (en) * 1989-01-17 1996-03-26 Alamar Biosciences Laboratory, Inc. Antibiotic and cytotoxic drug susceptibility assays using resazurin and poising agents
US6046021A (en) * 1995-04-12 2000-04-04 Biolog, Inc. Comparative phenotype analysis of two or more microorganisms using a plurality of substrates within a multiwell testing device
WO2001000209A1 (en) * 1999-06-29 2001-01-04 Smithkline Beecham Corporation Methods of use of fluoroquinolone compounds against bacteria
WO2001081920A2 (en) * 2000-04-20 2001-11-01 Biolog, Inc. Comparative phenotype analysis for assessment of biologically active compounds

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU740745B2 (en) * 1997-12-11 2001-11-15 Biochem Pharma Inc. Antiviral compounds
GB9908195D0 (en) * 1999-04-09 1999-06-02 Microbiological Res Authority Treatment of intracellular infection

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5366738A (en) * 1982-07-29 1994-11-22 Merck & Co., Inc. Controlled release drug dispersion delivery device
US5501959A (en) * 1989-01-17 1996-03-26 Alamar Biosciences Laboratory, Inc. Antibiotic and cytotoxic drug susceptibility assays using resazurin and poising agents
US5369095A (en) * 1990-02-14 1994-11-29 Alcon Laboratories, Inc. Compositions and method comprising substituted glycosides as mucus membrane permeation enhancers
US6046021A (en) * 1995-04-12 2000-04-04 Biolog, Inc. Comparative phenotype analysis of two or more microorganisms using a plurality of substrates within a multiwell testing device
WO2001000209A1 (en) * 1999-06-29 2001-01-04 Smithkline Beecham Corporation Methods of use of fluoroquinolone compounds against bacteria
WO2001081920A2 (en) * 2000-04-20 2001-11-01 Biolog, Inc. Comparative phenotype analysis for assessment of biologically active compounds

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
AUBEL DOMINIQUE ET AL: "Design of a novel mammalian screening system for the detection of bioavailable, non-cytotoxic streptogramin antibiotics", JOURNAL OF ANTIBIOTICS (TOKYO), vol. 54, no. 1, January 2001 (2001-01-01), pages 44 - 55, XP001160773, ISSN: 0021-8820 *
FISCHER AB: "Gentamycin as a bacteriocidal antibiotic in tissue culture", MED. MICROBIOL. IMMUNOL. (BERL.), vol. 161, no. 1, 1975, pages 23 - 39, XP008027169 *
SIMALA-GRANT J L ET AL: "Antibiotic susceptibility of attached and free-floating Helicobacter pylori.", THE JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. ENGLAND MAY 2001, vol. 47, no. 5, May 2001 (2001-05-01), pages 555 - 563, XP002268772, ISSN: 0305-7453 *

Also Published As

Publication number Publication date
DE10235967B4 (en) 2005-09-08
WO2004015416A2 (en) 2004-02-19
AU2003255282A1 (en) 2004-02-25
DE10235967A1 (en) 2004-02-26

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