WO2003095986A8 - System and methods for rapid and automated screening of cells - Google Patents

System and methods for rapid and automated screening of cells

Info

Publication number
WO2003095986A8
WO2003095986A8 PCT/IB2003/001821 IB0301821W WO03095986A8 WO 2003095986 A8 WO2003095986 A8 WO 2003095986A8 IB 0301821 W IB0301821 W IB 0301821W WO 03095986 A8 WO03095986 A8 WO 03095986A8
Authority
WO
WIPO (PCT)
Prior art keywords
methods
automated
implemented
segmentation
systems
Prior art date
Application number
PCT/IB2003/001821
Other languages
French (fr)
Other versions
WO2003095986A1 (en
Inventor
Peter Ramm
Yuriy Alexamdrov
Jerry Cybuch
Paul Donders
Carlos Zarate
Bohdan J Soltys
Original Assignee
Amersham Biosciences Niagara I
Peter Ramm
Yuriy Alexamdrov
Jerry Cybuch
Paul Donders
Carlos Zarate
Bohdan J Soltys
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Niagara I, Peter Ramm, Yuriy Alexamdrov, Jerry Cybuch, Paul Donders, Carlos Zarate, Bohdan J Soltys filed Critical Amersham Biosciences Niagara I
Priority to US10/514,925 priority Critical patent/US7469056B2/en
Priority to AU2003230069A priority patent/AU2003230069B2/en
Priority to JP2004503932A priority patent/JP2005525550A/en
Priority to CA2485602A priority patent/CA2485602C/en
Priority to EP03722911A priority patent/EP1504247A1/en
Publication of WO2003095986A1 publication Critical patent/WO2003095986A1/en
Publication of WO2003095986A8 publication Critical patent/WO2003095986A8/en
Priority to US12/253,275 priority patent/US7764822B2/en
Priority to US12/817,422 priority patent/US9057701B2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G01N15/1433—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5026—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell morphology
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/187—Segmentation; Edge detection involving region growing; involving region merging; involving connected component labelling
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1497—Particle shape
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/4603—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates from fish
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

Abstract

A system for performing automated cell screening in drug discovery includes an automated microscope (100), a fast autofocus device, and a digital imaging system (500). Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.
PCT/IB2003/001821 2002-05-14 2003-05-09 System and methods for rapid and automated screening of cells WO2003095986A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US10/514,925 US7469056B2 (en) 2002-05-14 2003-05-09 System and methods for rapid and automated screening of cells
AU2003230069A AU2003230069B2 (en) 2002-05-14 2003-05-09 System and methods for rapid and automated screening of cells
JP2004503932A JP2005525550A (en) 2002-05-14 2003-05-09 Rapid automated screening system and method for cells
CA2485602A CA2485602C (en) 2002-05-14 2003-05-09 System and methods for rapid and automated screening of cells
EP03722911A EP1504247A1 (en) 2002-05-14 2003-05-09 System and methods for rapid and automated screening of cells
US12/253,275 US7764822B2 (en) 2002-05-14 2008-10-17 System and methods for rapid and automated screening of cells
US12/817,422 US9057701B2 (en) 2002-05-14 2010-06-17 System and methods for rapid and automated screening of cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38082202P 2002-05-14 2002-05-14
US60/380,822 2002-05-14

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US10514925 A-371-Of-International 2003-05-09
US12/253,275 Division US7764822B2 (en) 2002-05-14 2008-10-17 System and methods for rapid and automated screening of cells
US12/253,275 Continuation US7764822B2 (en) 2002-05-14 2008-10-17 System and methods for rapid and automated screening of cells

Publications (2)

Publication Number Publication Date
WO2003095986A1 WO2003095986A1 (en) 2003-11-20
WO2003095986A8 true WO2003095986A8 (en) 2005-05-06

Family

ID=29420631

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2003/001821 WO2003095986A1 (en) 2002-05-14 2003-05-09 System and methods for rapid and automated screening of cells

Country Status (6)

Country Link
US (3) US7469056B2 (en)
EP (1) EP1504247A1 (en)
JP (1) JP2005525550A (en)
AU (1) AU2003230069B2 (en)
CA (1) CA2485602C (en)
WO (1) WO2003095986A1 (en)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0311185D0 (en) * 2003-05-15 2003-06-18 British Telecomm Image-size dependent facial caricaturing
US8719053B2 (en) 2003-07-17 2014-05-06 Ventana Medical Systems, Inc. Laboratory instrumentation information management and control network
US7860727B2 (en) 2003-07-17 2010-12-28 Ventana Medical Systems, Inc. Laboratory instrumentation information management and control network
JP4509702B2 (en) * 2004-08-31 2010-07-21 オリンパス株式会社 Method for measuring intracellular granular structure
US8084260B2 (en) * 2004-11-24 2011-12-27 Applied Biosystems, Llc Spectral calibration method and system for multiple instruments
JP4728025B2 (en) * 2005-03-29 2011-07-20 オリンパス株式会社 Cell image analyzer
JP4667944B2 (en) * 2005-04-20 2011-04-13 シスメックス株式会社 Image creation device
EP1775573A1 (en) * 2005-10-13 2007-04-18 Evotec Technologies GmbH Method for detection of contours in biological cells
US20090297015A1 (en) * 2005-10-13 2009-12-03 Fritz Jetzek Method for Detecting Contours in Images of Biological Cells
WO2007069414A1 (en) 2005-12-14 2007-06-21 The University Of Tokyo Method of analyzing line-shaped cell, method of analyzing nerve cell and apparatus and program for performing these methods
DE102005061834B4 (en) * 2005-12-23 2007-11-08 Ioss Intelligente Optische Sensoren & Systeme Gmbh Apparatus and method for optically examining a surface
US9275465B2 (en) * 2006-04-18 2016-03-01 Ge Healthcare Bio-Sciences Corp. System for preparing an image for segmentation
JP2007334141A (en) * 2006-06-16 2007-12-27 Sanyo Electric Co Ltd Culture observation system
US7706591B2 (en) 2006-07-13 2010-04-27 Cellomics, Inc. Neuronal profiling
US8073639B2 (en) * 2007-08-31 2011-12-06 Dh Technologies Development Pte. Ltd. Method for identifying a convolved peak
US8557587B2 (en) * 2009-01-07 2013-10-15 Xy, Llc Self-tuning, biologically modeled sorter
US8515143B2 (en) * 2009-01-09 2013-08-20 Dai Nippon Printing Co., Ltd. Embryo quality evaluation assistance system, embryo quality evaluation assistance apparatus and embryo quality evaluation assistance method
JPWO2010098105A1 (en) * 2009-02-26 2012-08-30 国立大学法人名古屋大学 Culture state evaluation apparatus, culture state evaluation method, incubator and program
US8385624B2 (en) * 2009-05-13 2013-02-26 David J. Charlot Automated transient image cytometry
US8310531B2 (en) * 2009-08-03 2012-11-13 Genetix Corporation Methods and apparatuses for processing fluorescence images
WO2011023203A1 (en) * 2009-08-24 2011-03-03 Abb Technology Ag Improved execution of real time applications with an automation controller
WO2011046511A1 (en) * 2009-10-13 2011-04-21 Agency For Science, Technology And Research A method and system for segmenting a liver object in an image
JP5820383B2 (en) * 2009-10-30 2015-11-24 コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． Three-dimensional analysis of lesions represented by image data
US9001200B2 (en) * 2010-01-12 2015-04-07 Bio-Rad Laboratories, Inc. Cell characterization using multiple focus planes
GB2478593B (en) * 2010-03-12 2017-05-31 Inst For Medical Informatics Optimising the initialization and convergence of active contours for segmentation of cell nuclei in histological sections
US8300938B2 (en) * 2010-04-09 2012-10-30 General Electric Company Methods for segmenting objects in images
CA2807144C (en) 2010-07-30 2019-05-28 Fundacao D. Anna Sommer Champalimaud E Dr. Carlos Montez Champalimaud Systems and methods for segmentation and processing of tissue images and feature extraction from same for treating, diagnosing, or predicting medical conditions
WO2012035504A1 (en) * 2010-09-14 2012-03-22 Ramot At Tel-Aviv University Ltd. Cell occupancy measurement
US8849057B2 (en) * 2011-05-19 2014-09-30 Foveon, Inc. Methods for digital image sharpening with noise amplification avoidance
US20130094750A1 (en) * 2011-10-12 2013-04-18 Tolga Tasdizen Methods and systems for segmentation of cells for an automated differential counting system
JP2013101484A (en) * 2011-11-08 2013-05-23 Sony Corp Image processing apparatus, image processing method and program
WO2013102949A1 (en) * 2012-01-04 2013-07-11 株式会社知能情報システム Image processing method, image processing device, image processing program, and memory medium
US9518914B2 (en) * 2012-09-24 2016-12-13 Brigham And Women's Hospital, Inc. Portal and method for management of dialysis therapy
JP5698208B2 (en) * 2012-11-30 2015-04-08 株式会社Ｓｃｒｅｅｎホールディングス Image processing apparatus, image processing method, and image processing program
WO2014093980A1 (en) 2012-12-14 2014-06-19 Vala Sciences, Inc. Analysis of action potentials, transients, and ion flux in excitable cells
CN105378453B (en) 2012-12-19 2018-09-25 皇家飞利浦有限公司 The system and method for classification for the particle in fluid sample
DE102013218437A1 (en) * 2013-09-13 2015-03-19 Siemens Aktiengesellschaft Method for automatic or semi-automatic segmentation and device
JP6195373B2 (en) * 2013-12-19 2017-09-13 株式会社Ｓｃｒｅｅｎホールディングス Imaging apparatus and imaging method
PL232619B1 (en) * 2014-01-08 2019-07-31 Inst Chemicznej Przerobki Wegla Method for determining morphology of cokes and carbonizates
US10311550B2 (en) * 2014-03-27 2019-06-04 Noritsu Precision Co., Ltd. Image processing device for eliminating graininess of image
US10473906B2 (en) * 2014-05-30 2019-11-12 Nikon Corporation Microscope
EP2985719A1 (en) * 2014-08-15 2016-02-17 IMEC vzw A system and method for cell recognition
US9939623B2 (en) * 2015-10-19 2018-04-10 Molecular Devices, Llc Microscope system with transillumination-based autofocusing for photoluminescence imaging
US9971966B2 (en) * 2016-02-26 2018-05-15 Google Llc Processing cell images using neural networks
WO2018111765A1 (en) 2016-12-12 2018-06-21 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
JP7042045B2 (en) * 2017-08-10 2022-03-25 シスメックス株式会社 Sample analyzer
DE102017009804A1 (en) * 2017-10-20 2019-04-25 Vermicon Ag Method for evaluating microscopic samples and apparatus for carrying out this method
EP3729329A1 (en) * 2017-12-22 2020-10-28 Ventana Medical Systems, Inc. System and method for classifying cells in tissue images based on membrane features
EP3811287A2 (en) * 2018-06-19 2021-04-28 MetaSystems Hard & Software GmbH System and method for detection and classification of objects of interest in microscope images by supervised machine learning
CN108961250B (en) * 2018-07-20 2023-02-07 中国科学院深圳先进技术研究院 Target object statistical method, device, terminal and storage medium
US10885631B2 (en) * 2019-02-01 2021-01-05 Essen Instruments, Inc. Label-free cell segmentation using phase contrast and brightfield imaging
US11803963B2 (en) 2019-02-01 2023-10-31 Sartorius Bioanalytical Instruments, Inc. Computational model for analyzing images of a biological specimen
US11164039B2 (en) 2019-10-23 2021-11-02 International Business Machines Corporation Framework for few-shot temporal action localization
CN211857087U (en) * 2020-02-24 2020-11-03 宁波激智科技股份有限公司 Interference reducing collimation film
WO2022023956A1 (en) * 2020-07-31 2022-02-03 The Joan and Irwin Jacobs Technion-Cornell Institute System and method for on-phase microscopy
CN113111926A (en) * 2021-03-31 2021-07-13 南京华晟医学检验实验室有限公司 Abnormal cervical blood cell screening method based on TCT (TCT) slide
CN115165710B (en) * 2022-09-08 2022-11-29 珠海圣美生物诊断技术有限公司 Rapid scanning method and device for cells
CN116698679B (en) * 2023-07-27 2023-12-19 中国食品药品检定研究院 Real-time particle filtering effect measuring device and system

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4741043B1 (en) 1985-11-04 1994-08-09 Cell Analysis Systems Inc Method of and apparatus for image analyses of biological specimens
US5281517A (en) 1985-11-04 1994-01-25 Cell Analysis Systems, Inc. Methods for immunoploidy analysis
US4881808A (en) 1988-02-10 1989-11-21 Intelligent Surgical Lasers Imaging system for surgical lasers
US4965725B1 (en) 1988-04-08 1996-05-07 Neuromedical Systems Inc Neural network based automated cytological specimen classification system and method
US5072382A (en) 1989-10-02 1991-12-10 Kamentsky Louis A Methods and apparatus for measuring multiple optical properties of biological specimens
US5162641A (en) 1991-02-19 1992-11-10 Phoenix Laser Systems, Inc. System and method for detecting, correcting and measuring depth movement of target tissue in a laser surgical system
US5548661A (en) * 1991-07-12 1996-08-20 Price; Jeffrey H. Operator independent image cytometer
US5733721A (en) 1992-11-20 1998-03-31 The Board Of Regents Of The University Of Oklahoma Cell analysis method using quantitative fluorescence image analysis
US6130475A (en) * 1993-12-07 2000-10-10 International Business Machines Corporation Clock distribution system for synchronous circuit assemblies
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5932872A (en) * 1994-07-01 1999-08-03 Jeffrey H. Price Autofocus system for scanning microscopy having a volume image formation
US5627908A (en) 1994-09-20 1997-05-06 Neopath, Inc. Method for cytological system dynamic normalization
AU3586195A (en) 1994-09-20 1996-04-09 Neopath, Inc. Apparatus for automated identification of cell groupings on a biological specimen
US6252979B1 (en) 1995-06-07 2001-06-26 Tripath Imaging, Inc. Interactive method and apparatus for sorting biological specimens
US6718053B1 (en) * 1996-11-27 2004-04-06 Chromavision Medical Systems, Inc. Method and apparatus for automated image analysis of biological specimens
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US6103479A (en) 1996-05-30 2000-08-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
JPH09325277A (en) * 1996-06-04 1997-12-16 Nikon Corp Focus detector
JP3837165B2 (en) 1996-08-16 2006-10-25 アマーシャム バイオサイエンセズ ナイアガラ，インク． Digital imaging system for testing in well plates, gels and blots
US6307957B1 (en) 1997-02-28 2001-10-23 Electro-Optical Sciences Inc Multispectral imaging and characterization of biological tissue
DE19713362A1 (en) 1997-03-29 1998-10-01 Zeiss Carl Jena Gmbh Confocal microscopic arrangement
US6271036B1 (en) 1997-07-16 2001-08-07 University Of Medicine & Dentistry Of New Jersey Method for evaluating cytopathology specimens
WO2000037984A2 (en) * 1998-12-21 2000-06-29 Evotec Biosystems Ag Positioning of the measuring volume in a scanning microscopic method
US6130745A (en) 1999-01-07 2000-10-10 Biometric Imaging, Inc. Optical autofocus for use with microtiter plates
US6876760B1 (en) * 2000-12-04 2005-04-05 Cytokinetics, Inc. Classifying cells based on information contained in cell images
US6986993B1 (en) * 1999-08-05 2006-01-17 Cellomics, Inc. System for cell-based screening
ATE309528T1 (en) 1999-08-05 2005-11-15 Cellomics Inc OPTICAL SYSTEM ANALYSIS FOR CELLS

Also Published As

Publication number Publication date
AU2003230069A1 (en) 2003-11-11
US7469056B2 (en) 2008-12-23
US20090074283A1 (en) 2009-03-19
US7764822B2 (en) 2010-07-27
CA2485602C (en) 2013-10-01
US9057701B2 (en) 2015-06-16
CA2485602A1 (en) 2003-11-20
AU2003230069B2 (en) 2008-11-13
US20060257013A1 (en) 2006-11-16
EP1504247A1 (en) 2005-02-09
WO2003095986A1 (en) 2003-11-20
JP2005525550A (en) 2005-08-25
US20100254590A1 (en) 2010-10-07

Similar Documents

Publication Publication Date Title
WO2003095986A8 (en) System and methods for rapid and automated screening of cells
US10281386B2 (en) Automated testing apparatus
CA2253850A1 (en) Method and apparatus for automatically detecting malignancy-associated changes
CA2299707A1 (en) System and method for automatically detecting malignant cells and cells having malignancy-associated changes
WO2011028818A3 (en) High throughput multichannel reader and uses thereof
Claude et al. DESI mass spectrometry imaging (MSI)
WO1995010036A3 (en) Cytological screening method
WO2006074290A3 (en) Optical flow based tilt sensor
SG142124A1 (en) Image texture retrieving method and apparatus thereof
WO2003106157A3 (en) Automated slide staining apparatus
GB0312481D0 (en) Antibodies
EP1447699A3 (en) Microscope system and method
DK1588317T3 (en) Maintaining video scene background using change detection and classification
JPS5661650A (en) Analyzing device of cell
WO2004103007A3 (en) Apparatus and method for providing location information
WO2007015374A3 (en) Image processing apparatus and image processing program
EP1739719A2 (en) Increased depth of field for high resolution imaging for a matrix-based ion source
WO2004025569A3 (en) Tissue image analysis for cell classification and laser capture microdissection
WO2004061742A3 (en) Medical data analysis method and apparatus incorporating in vitro test data
WO2006132708A3 (en) Automated position control of a surface array relative to a liquid microjunction surface sampler
EP1260807B1 (en) Consumable for laser capture microdissection
WO2004006985A3 (en) Assaying and imaging system identifying traits of biological specimens
JP2004126574A5 (en)
CN110806407A (en) Labview-based two-dimensional material scanning and vision processing system and method
WO2000072258A3 (en) Method and system for general purpose analysis of experimental data

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2003230069

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2485602

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2006257013

Country of ref document: US

Ref document number: 2004503932

Country of ref document: JP

Ref document number: 10514925

Country of ref document: US

REEP Request for entry into the european phase

Ref document number: 2003722911

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2003722911

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003722911

Country of ref document: EP

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: IN PCT GAZETTE 47/2003 UNDER (72, 75) ADD "SOLTYS, BOHDAN, J. ¢CA/CA!; 485 BOHEMIA CRESCENT, OAKVILLE, ONTARIO L6J 2K7 (CA)."

WWP Wipo information: published in national office

Ref document number: 10514925

Country of ref document: US