WO2003092846A3 - Plastic microfluidics enabling two-dimensional separations of biological molecules - Google Patents

Plastic microfluidics enabling two-dimensional separations of biological molecules Download PDF

Info

Publication number
WO2003092846A3
WO2003092846A3 PCT/US2003/013580 US0313580W WO03092846A3 WO 2003092846 A3 WO2003092846 A3 WO 2003092846A3 US 0313580 W US0313580 W US 0313580W WO 03092846 A3 WO03092846 A3 WO 03092846A3
Authority
WO
WIPO (PCT)
Prior art keywords
dimension
microfluidics
enabling
plastic
biological molecules
Prior art date
Application number
PCT/US2003/013580
Other languages
French (fr)
Other versions
WO2003092846A2 (en
Inventor
Cheng Sheng Lee
Don Devoe
Original Assignee
Cheng Sheng Lee
Don Devoe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/135,385 external-priority patent/US6929730B2/en
Priority claimed from US10/135,386 external-priority patent/US6974526B2/en
Application filed by Cheng Sheng Lee, Don Devoe filed Critical Cheng Sheng Lee
Priority to AU2003232028A priority Critical patent/AU2003232028A1/en
Publication of WO2003092846A2 publication Critical patent/WO2003092846A2/en
Publication of WO2003092846A3 publication Critical patent/WO2003092846A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/54—Heating or cooling apparatus; Heat insulating devices using spatial temperature gradients

Abstract

One embodiment (Figure 10) of the invention relates to a microfiuidc apparatus for performing two dimensional biomolecular separations. According to one aspect of the invention, after a first dimension separation in a first microchannel, the sample material is electrokinetically and simultaneoulsy transferred to an array of microchannels in the second dimension. Preferably any separation accomplished in the first dimension is completely retained upon transfer to the second dimension. According to another aspect of the invention, the separation in the second dimension is performed using a temperature gradient (e.g., a spatial or temporal temperature gradient).
PCT/US2003/013580 2002-05-01 2003-05-01 Plastic microfluidics enabling two-dimensional separations of biological molecules WO2003092846A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003232028A AU2003232028A1 (en) 2002-05-01 2003-05-01 Plastic microfluidics enabling two-dimensional separations of biological molecules

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10/135,386 2002-05-01
US10/135,385 US6929730B2 (en) 2001-05-01 2002-05-01 Two dimensional microfluidic gene scanner
US10/135,385 2002-05-01
US10/135,386 US6974526B2 (en) 2001-05-01 2002-05-01 Plastic microfluidics enabling two-dimensional protein separations in proteome analysis

Publications (2)

Publication Number Publication Date
WO2003092846A2 WO2003092846A2 (en) 2003-11-13
WO2003092846A3 true WO2003092846A3 (en) 2009-06-18

Family

ID=29406239

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/013580 WO2003092846A2 (en) 2002-05-01 2003-05-01 Plastic microfluidics enabling two-dimensional separations of biological molecules

Country Status (2)

Country Link
AU (1) AU2003232028A1 (en)
WO (1) WO2003092846A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7815783B2 (en) * 2004-03-17 2010-10-19 Bio-Rad Laboratories, Inc. Multi-compartment filter and method of filtering using same
EP1712903B1 (en) * 2005-04-11 2015-07-15 Roche Diagnostics GmbH Method for Integrated 2D gel electrophoresis
EP1801573A1 (en) 2005-12-21 2007-06-27 Boehringer Mannheim Gmbh Method and apparatus for parallel two-dimensional electrophoresis
GB2440749B (en) * 2006-05-26 2011-04-06 Marc Baumann Multi-dimensional analysis
US7744762B2 (en) 2006-08-24 2010-06-29 Virginia Tech Intellectual Properties, Inc. Microfluidic devices and methods facilitating high-throughput, on-chip detection and separation techniques
DE102016211355A1 (en) * 2016-06-24 2017-12-28 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Analysis system and method for performing an analysis

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
US6406604B1 (en) * 1999-11-08 2002-06-18 Norberto A. Guzman Multi-dimensional electrophoresis apparatus
US6540896B1 (en) * 1998-08-05 2003-04-01 Caliper Technologies Corp. Open-Field serial to parallel converter

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
US6540896B1 (en) * 1998-08-05 2003-04-01 Caliper Technologies Corp. Open-Field serial to parallel converter
US6406604B1 (en) * 1999-11-08 2002-06-18 Norberto A. Guzman Multi-dimensional electrophoresis apparatus

Also Published As

Publication number Publication date
AU2003232028A8 (en) 2009-07-30
WO2003092846A2 (en) 2003-11-13
AU2003232028A1 (en) 2003-11-17

Similar Documents

Publication Publication Date Title
Sia et al. Microfluidic devices fabricated in poly (dimethylsiloxane) for biological studies
WO2003077851A3 (en) Compounds and methods for analyzing the proteome
Lee et al. Microfabricated plastic chips by hot embossing methods and their applications for DNA separation and detection
Bousse et al. Electrokinetically controlled microfluidic analysis systems
Cabodi et al. Entropic recoil separation of long DNA molecules
US6613560B1 (en) PCR microreactor for amplifying DNA using microquantities of sample fluid
AU2002359508A8 (en) Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
US20120115189A1 (en) Microfluidic and nanofluidic devices, systems, and applications
AU2003302264A1 (en) Assay apparatus and method using microfluidic arrays
Kricka et al. Micromachined analytical devices: microchips for semen testing
SG120120A1 (en) Microfluidic system for analyzing nucleic acids
ES2185709T3 (en) APPARATUS AND METHOD FOR PERFORMING MICROFLUIDIC HANDLING FOR CHEMICAL ANALYSIS AND SYNTHESIS.
AU2002367839A1 (en) Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
AU2002353107A1 (en) Microfluidic devices and methods for two-dimensional separations
WO1999066318A8 (en) A microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same
WO2005047904A3 (en) High-density amine-functionalized surface
WO2005068628A3 (en) Methods for nucleic acid isolation and kits using solid phase material
Morbioli et al. Rapid and low-cost development of microfluidic devices using wax printing and microwave treatment
Chiu A microfluidics platform for cell fusion
WO2003092846A3 (en) Plastic microfluidics enabling two-dimensional separations of biological molecules
ATE320311T1 (en) METHOD FOR PRODUCING A MICROFLUIDIC DEVICE
DK1476549T3 (en) Method for conformation-dependent separation of non-circular nucleic acids
ATE384117T1 (en) USE OF A MINIATURE DEVICE FOR SEPARATION AND ISOLATION OF BIOLOGICAL OBJECTS AND SEPARATION METHOD
WO2003087773A3 (en) Multiplexed capillary electrophoresis systems
WO2002029396A3 (en) Method and device for carrying out 2d electrophoresis in large gels

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP