WO2003066851A3 - Irreversible immobilization of diisopropylfluorophophatase into polyurethane coatings - Google Patents

Irreversible immobilization of diisopropylfluorophophatase into polyurethane coatings Download PDF

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Publication number
WO2003066851A3
WO2003066851A3 PCT/US2002/023473 US0223473W WO03066851A3 WO 2003066851 A3 WO2003066851 A3 WO 2003066851A3 US 0223473 W US0223473 W US 0223473W WO 03066851 A3 WO03066851 A3 WO 03066851A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
polyurethane coatings
diisopropylfluorophophatase
ecc
immobilization
Prior art date
Application number
PCT/US2002/023473
Other languages
French (fr)
Other versions
WO2003066851A2 (en
Inventor
Alan J Russell
Geraldine F Drevon
Original Assignee
Univ Pittsburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Pittsburgh filed Critical Univ Pittsburgh
Priority to BR0211402-0A priority Critical patent/BR0211402A/en
Priority to KR10-2004-7001032A priority patent/KR20040023672A/en
Priority to AU2002365908A priority patent/AU2002365908A1/en
Priority to EP02806756A priority patent/EP1409663A2/en
Priority to JP2003566201A priority patent/JP2005516620A/en
Priority to MXPA04000736A priority patent/MXPA04000736A/en
Priority to CA002454826A priority patent/CA2454826A1/en
Publication of WO2003066851A2 publication Critical patent/WO2003066851A2/en
Publication of WO2003066851A3 publication Critical patent/WO2003066851A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres

Landscapes

  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Paints Or Removers (AREA)

Abstract

This invention provides a method of irreversibly immobilizing an enzyme into polyurethane coatings. This invention also provides for an enzyme-containing coating having a degree of immobilization of the enzyme of approximately 100%. The synthesis of waterborne polyurethane coatings in the presence of enzyme has enabled the irreversible attachment of the enzyme to the polymeric matrix. The distribution of immobilized enzyme as well as activity retention are homogeneous within the coating. Decreasing ECC hydrophobicity, via the use of a less hydrophobic polyisocyanate prepolymer during polymerization, significantly enhanced the intrinsic activity of the ECC.
PCT/US2002/023473 2001-07-24 2002-07-24 Irreversible immobilization of diisopropylfluorophophatase into polyurethane coatings WO2003066851A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
BR0211402-0A BR0211402A (en) 2001-07-24 2002-07-24 Irreversibly Immobilization of Diisopropylfluorophosphatase in Polyurethane Coatings
KR10-2004-7001032A KR20040023672A (en) 2001-07-24 2002-07-24 Irreversible immobilization of diisopropylfluorophophatase into polyurethane coatings
AU2002365908A AU2002365908A1 (en) 2001-07-24 2002-07-24 Irreversible immobilization of diisopropylfluorophophatase into polyurethane coatings
EP02806756A EP1409663A2 (en) 2001-07-24 2002-07-24 Irreversible immobilization of diisopropylfluorophophatase into polyurethane coatings
JP2003566201A JP2005516620A (en) 2001-07-24 2002-07-24 Irreversible immobilization of diisopropylfluorophosphatase in polyurethane coatings
MXPA04000736A MXPA04000736A (en) 2001-07-24 2002-07-24 Irreversible immobilization of diisopropylfluorophophatase into polyurethane coatings.
CA002454826A CA2454826A1 (en) 2001-07-24 2002-07-24 Irreversible immobilization of diisopropylfluorophosphatase into polyurethane coatings

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30745001P 2001-07-24 2001-07-24
US60/307,450 2001-07-24

Publications (2)

Publication Number Publication Date
WO2003066851A2 WO2003066851A2 (en) 2003-08-14
WO2003066851A3 true WO2003066851A3 (en) 2003-12-04

Family

ID=27734194

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/023473 WO2003066851A2 (en) 2001-07-24 2002-07-24 Irreversible immobilization of diisopropylfluorophophatase into polyurethane coatings

Country Status (9)

Country Link
US (1) US6905733B2 (en)
EP (1) EP1409663A2 (en)
JP (1) JP2005516620A (en)
KR (1) KR20040023672A (en)
AU (1) AU2002365908A1 (en)
BR (1) BR0211402A (en)
CA (1) CA2454826A1 (en)
MX (1) MXPA04000736A (en)
WO (1) WO2003066851A2 (en)

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Also Published As

Publication number Publication date
EP1409663A2 (en) 2004-04-21
US20030129314A1 (en) 2003-07-10
CA2454826A1 (en) 2003-08-14
MXPA04000736A (en) 2005-02-17
US6905733B2 (en) 2005-06-14
KR20040023672A (en) 2004-03-18
WO2003066851A2 (en) 2003-08-14
AU2002365908A1 (en) 2003-09-02
AU2002365908A8 (en) 2003-09-02
BR0211402A (en) 2005-02-22
JP2005516620A (en) 2005-06-09

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