WO2003046512A3 - Method for amplification of molecular bio-assay signals - Google Patents

Method for amplification of molecular bio-assay signals Download PDF

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Publication number
WO2003046512A3
WO2003046512A3 PCT/US2002/038104 US0238104W WO03046512A3 WO 2003046512 A3 WO2003046512 A3 WO 2003046512A3 US 0238104 W US0238104 W US 0238104W WO 03046512 A3 WO03046512 A3 WO 03046512A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplification
bioassays
molecules
assay signals
molecule
Prior art date
Application number
PCT/US2002/038104
Other languages
French (fr)
Other versions
WO2003046512A2 (en
Inventor
Fengchun Ye
Original Assignee
Royce Technologies Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002363703A external-priority patent/CA2363703A1/en
Application filed by Royce Technologies Llc filed Critical Royce Technologies Llc
Priority to AU2002363953A priority Critical patent/AU2002363953A1/en
Publication of WO2003046512A2 publication Critical patent/WO2003046512A2/en
Publication of WO2003046512A3 publication Critical patent/WO2003046512A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

Disclosed are reagents and a method for effective in vitro amplification of bio-assay signals. The method makes use of a pair of 'end-to-end' complementary oligonucleotide primers to continuously form a double-stranded and highly repetitive hybrid molecule. Since this hybrid molecule is covalently linked and added to the probing molecule in the bioassays, and is also compatibly labeled, it can amplify the detection signals hundreds of times within a short period. The method can have very broad applications in bioassays using nucleic acid hybridization, immunochemical detection, and other specific interactions between two molecules or between molecules and cells.
PCT/US2002/038104 2001-11-23 2002-11-25 Method for amplification of molecular bio-assay signals WO2003046512A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002363953A AU2002363953A1 (en) 2001-11-23 2002-11-25 Method for amplification of molecular bio-assay signals

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA002363703A CA2363703A1 (en) 2001-11-23 2001-11-23 Method for amplification of molecular bio-assay signals
CA2,363,703 2001-11-23
US10/299,066 2002-11-18
US10/299,066 US20030124604A1 (en) 2001-11-23 2002-11-18 Method for amplification of molecular bio-assay signals

Publications (2)

Publication Number Publication Date
WO2003046512A2 WO2003046512A2 (en) 2003-06-05
WO2003046512A3 true WO2003046512A3 (en) 2004-02-05

Family

ID=25682800

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/038104 WO2003046512A2 (en) 2001-11-23 2002-11-25 Method for amplification of molecular bio-assay signals

Country Status (2)

Country Link
AU (1) AU2002363953A1 (en)
WO (1) WO2003046512A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009026651A1 (en) * 2007-08-30 2009-03-05 Commonwealth Scientific And Industrial Research Organisation A method of detecting a nucleic acid

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LIZARDI ET AL.: "Mutation detection and single-molecul counting using isothermal rolling-circle amplification", NATURE GENETICS, vol. 19, July 1998 (1998-07-01), pages 225 - 232, XP002922815 *
SCHWEITZER ET AL.: "Immunoassays with rolling circle DNA amplification: A versatile platform for ultra sensitive antigen detection", PROC. NATL. ACAD. SCI. USA, vol. 97, no. 18, 29 August 2000 (2000-08-29), pages 10113 - 10119, XP002207138 *

Also Published As

Publication number Publication date
AU2002363953A1 (en) 2003-06-10
AU2002363953A8 (en) 2003-06-10
WO2003046512A2 (en) 2003-06-05

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