WO2003044187A3 - Emulsion compositions - Google Patents

Emulsion compositions Download PDF

Info

Publication number
WO2003044187A3
WO2003044187A3 PCT/GB2002/005216 GB0205216W WO03044187A3 WO 2003044187 A3 WO2003044187 A3 WO 2003044187A3 GB 0205216 W GB0205216 W GB 0205216W WO 03044187 A3 WO03044187 A3 WO 03044187A3
Authority
WO
WIPO (PCT)
Prior art keywords
emulsion
emulsion compositions
vitro
eukaryotic
hydrophilic phase
Prior art date
Application number
PCT/GB2002/005216
Other languages
French (fr)
Other versions
WO2003044187A2 (en
Inventor
Philipp Holliger
Farid Ghadessy
Original Assignee
Medical Res Council
Philipp Holliger
Farid Ghadessy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA2467215A priority Critical patent/CA2467215C/en
Priority to EP02777570A priority patent/EP1446504B1/en
Priority to DE60228607T priority patent/DE60228607D1/en
Priority to AU2002339193A priority patent/AU2002339193B2/en
Priority to DK02777570T priority patent/DK1446504T3/en
Priority to JP2003545810A priority patent/JP4503290B2/en
Application filed by Medical Res Council, Philipp Holliger, Farid Ghadessy filed Critical Medical Res Council
Publication of WO2003044187A2 publication Critical patent/WO2003044187A2/en
Publication of WO2003044187A3 publication Critical patent/WO2003044187A3/en
Priority to US10/844,720 priority patent/US7429467B2/en
Priority to US10/866,392 priority patent/US7622280B2/en
Priority to US12/079,834 priority patent/US7655435B2/en
Priority to US12/587,432 priority patent/US8153402B2/en
Priority to US12/694,071 priority patent/US8153364B2/en
Priority to US13/408,071 priority patent/US20120184011A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/24—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/44—Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/167—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction with an outer layer or coating comprising drug; with chemically bound drugs or non-active substances on their surface
    • A61K9/1676—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction with an outer layer or coating comprising drug; with chemically bound drugs or non-active substances on their surface having a drug-free core with discrete complete coating layer containing drug
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Abstract

An emulsion is useful in allowing a wide variety of gene products to be expressed via eukaryotic in vitro expression. The emulsion comprises a silicone based surfactant, a hydrophobic phase and a hydrophilic phase; wherein the hydrophilic phase comprises a plurality of compartments containing a functional in vitro eukaryotic expression system.
PCT/GB2002/005216 2001-11-16 2002-11-18 Emulsion compositions WO2003044187A2 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
JP2003545810A JP4503290B2 (en) 2001-11-16 2002-11-18 Emulsion composition
EP02777570A EP1446504B1 (en) 2001-11-16 2002-11-18 Emulsion compositions
DE60228607T DE60228607D1 (en) 2001-11-16 2002-11-18 EMULSION-BASED COMPOSITIONS
AU2002339193A AU2002339193B2 (en) 2001-11-16 2002-11-18 Emulsion compositions
DK02777570T DK1446504T3 (en) 2001-11-16 2002-11-18 emulsion
CA2467215A CA2467215C (en) 2001-11-16 2002-11-18 Emulsion compositions
US10/844,720 US7429467B2 (en) 2001-11-16 2004-05-13 Emulsion compositions
US10/866,392 US7622280B2 (en) 2001-11-16 2004-06-11 Emulsion compositions
US12/079,834 US7655435B2 (en) 2001-11-16 2008-03-28 Emulsion compositions
US12/587,432 US8153402B2 (en) 2001-11-16 2009-10-06 Emulsion compositions
US12/694,071 US8153364B2 (en) 2001-11-16 2010-01-26 Emulsion compositions
US13/408,071 US20120184011A1 (en) 2001-11-16 2012-02-29 Emulsion Compositions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0127564.3A GB0127564D0 (en) 2001-11-16 2001-11-16 Emulsion compositions
GB0127564.3 2001-11-16

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US10844720 A-371-Of-International 2002-11-18
US10/844,720 Continuation US7429467B2 (en) 2001-11-16 2004-05-13 Emulsion compositions
US12/079,834 Division US7655435B2 (en) 2001-11-16 2008-03-28 Emulsion compositions

Publications (2)

Publication Number Publication Date
WO2003044187A2 WO2003044187A2 (en) 2003-05-30
WO2003044187A3 true WO2003044187A3 (en) 2003-09-04

Family

ID=9925928

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/005216 WO2003044187A2 (en) 2001-11-16 2002-11-18 Emulsion compositions

Country Status (11)

Country Link
US (6) US7429467B2 (en)
EP (2) EP1446504B1 (en)
JP (2) JP4503290B2 (en)
AT (2) ATE406460T1 (en)
AU (1) AU2002339193B2 (en)
CA (1) CA2467215C (en)
DE (2) DE60222307T2 (en)
DK (1) DK1446504T3 (en)
ES (2) ES2312630T3 (en)
GB (1) GB0127564D0 (en)
WO (1) WO2003044187A2 (en)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8871444B2 (en) 2004-10-08 2014-10-28 Medical Research Council In vitro evolution in microfluidic systems
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US9228229B2 (en) 2010-02-12 2016-01-05 Raindance Technologies, Inc. Digital analyte analysis
US9273308B2 (en) 2006-05-11 2016-03-01 Raindance Technologies, Inc. Selection of compartmentalized screening method
US9328344B2 (en) 2006-01-11 2016-05-03 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9440232B2 (en) 2007-02-06 2016-09-13 Raindance Technologies, Inc. Manipulation of fluids and reactions in microfluidic systems
US9448172B2 (en) 2003-03-31 2016-09-20 Medical Research Council Selection by compartmentalised screening
US9498759B2 (en) 2004-10-12 2016-11-22 President And Fellows Of Harvard College Compartmentalized screening by microfluidic control
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets

Families Citing this family (287)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
ATE358177T1 (en) 1997-07-07 2007-04-15 Medical Res Council AN IN VITRO SORTING PROCESS
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6770441B2 (en) * 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
ES2342665T3 (en) 2003-01-29 2010-07-12 454 Corporation SEQUENCING FROM TWO EXTREME.
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
JP5183063B2 (en) 2003-07-05 2013-04-17 ザ ジョンズ ホプキンス ユニバーシティ Methods and compositions for detection and enumeration of genetic variations
GB0325653D0 (en) 2003-11-03 2003-12-10 Medical Res Council CST emulsions
AU2004288017B2 (en) * 2003-11-03 2009-10-08 United Kingdom Research And Innovation Polymerase
CA2553833C (en) 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
EP2233583A1 (en) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
EP2230315A1 (en) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
JP2009538123A (en) * 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー Reagents, methods and libraries for gel-free bead-based sequencing
WO2007149432A2 (en) * 2006-06-19 2007-12-27 The Johns Hopkins University Single-molecule pcr on microparticles in water-in-oil emulsions
US7682791B2 (en) * 2006-10-29 2010-03-23 Macevicz Stephen C Method of generating nested sets of double stranded DNA circles
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US7883265B2 (en) * 2007-06-01 2011-02-08 Applied Biosystems, Llc Devices, systems, and methods for preparing emulsions
US20090105959A1 (en) * 2007-06-01 2009-04-23 Braverman Michael S System and method for identification of individual samples from a multiplex mixture
CN101802218A (en) * 2007-06-28 2010-08-11 454生命科学公司 Ystem and method for adaptive reagent control in nucleic acid sequencing
US20100304378A1 (en) * 2007-09-17 2010-12-02 Universite De Strasbourg Method for Detecting or Quantifying a Truncating Mutation
CN101827613A (en) * 2007-09-27 2010-09-08 免疫疫苗技术有限公司 Use of liposomes in a carrier comprising a continuous hydrophobic phase for delivery of polynucleotides in vivo
EP2212434A1 (en) * 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase ligation sequencing
US8685675B2 (en) 2007-11-27 2014-04-01 Human Genetic Signatures Pty. Ltd. Enzymes for amplification and copying bisulphite modified nucleic acids
WO2009100080A1 (en) 2008-02-04 2009-08-13 Life Technologies Corporation Composition and method for sequencing nucleic acid
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP2296696B1 (en) 2008-06-05 2014-08-27 ImmunoVaccine Technologies Inc. Compositions comprising liposomes, an antigen, a polynucleotide and a carrier comprising a continuous phase of a hydrophobic substance
US7888034B2 (en) 2008-07-01 2011-02-15 454 Life Sciences Corporation System and method for detection of HIV tropism variants
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US20100261189A1 (en) 2008-10-03 2010-10-14 Roche Molecular Systems, Inc. System and method for detection of HLA Variants
US20100087325A1 (en) * 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
EP3415235A1 (en) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation of microfluidic droplets
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
WO2010117461A2 (en) * 2009-04-08 2010-10-14 Applied Biosystems, Llc System and method for preparing and using bulk emulsion
EP2940153B1 (en) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US8609339B2 (en) 2009-10-09 2013-12-17 454 Life Sciences Corporation System and method for emulsion breaking and recovery of biological elements
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
WO2011066476A1 (en) 2009-11-25 2011-06-03 Quantalife, Inc. Methods and compositions for detecting genetic material
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US8399198B2 (en) 2010-03-02 2013-03-19 Bio-Rad Laboratories, Inc. Assays with droplets transformed into capsules
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
EP2550351A4 (en) 2010-03-25 2014-07-09 Quantalife Inc Detection system for droplet-based assays
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
CN102858965A (en) 2010-04-30 2013-01-02 霍夫曼-拉罗奇有限公司 System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
US20110287432A1 (en) 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
US20120322665A1 (en) 2010-10-08 2012-12-20 454 Life Sciences Corporation System and method for detection of hiv-1 clades and recombinants of the reverse transcriptase and protease regions
WO2012058647A1 (en) 2010-10-28 2012-05-03 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
CA3140602C (en) 2010-11-01 2023-10-03 Bio-Rad Laboratories, Inc. System for forming emulsions
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
AU2012214312A1 (en) 2011-02-09 2013-08-22 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US20120244523A1 (en) 2011-03-25 2012-09-27 454 Life Sciences Corporation System and Method for Detection of HIV Integrase Variants
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
SI3623481T1 (en) 2011-09-23 2022-01-31 Illumina, Inc. Compositions for nucleic acid sequencing
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
EP3495499A1 (en) 2011-12-30 2019-06-12 Quest Diagnostics Investments Incorporated Nucleic acid analysis using emulsion pcr
JP6196241B2 (en) 2012-02-02 2017-09-13 インベンラ， インコーポレイテッド High-throughput screening of biologically active polypeptides
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
US9193996B2 (en) 2012-04-03 2015-11-24 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
CN104471075B (en) 2012-04-19 2018-06-22 生命技术公司 Nucleic acid amplification
EP3095879B1 (en) 2012-04-19 2018-09-19 Life Technologies Corporation Nucleic acid amplification
US10192024B2 (en) 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9422602B2 (en) 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
EP2895620B1 (en) 2012-09-11 2017-08-02 Life Technologies Corporation Nucleic acid amplification
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP5878113B2 (en) * 2012-12-28 2016-03-08 シスメックス株式会社 Nucleic acid amplification method and kit for nucleic acid amplification reaction
KR102190198B1 (en) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. Polynucleotide barcode generation
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
EP2971071B1 (en) 2013-03-15 2018-02-28 Illumina, Inc. Enzyme-linked nucleotides
EP2971160B1 (en) 2013-03-15 2018-05-30 Bio-Rad Laboratories, Inc. Digital assays for mutation detection
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
ES2700529T3 (en) 2013-07-01 2019-02-18 Illumina Inc Functionalization of surface without catalyst and polymer graft
ES2719579T3 (en) 2013-07-03 2019-07-11 Illumina Inc System for sequencing by orthogonal synthesis
WO2015013681A1 (en) 2013-07-25 2015-01-29 Bio-Rad Laboratories, Inc. Genetic assays
US9410977B2 (en) 2013-08-08 2016-08-09 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
EP2840148B1 (en) 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Methods for nucleic acid amplification
EP2848698A1 (en) 2013-08-26 2015-03-18 F. Hoffmann-La Roche AG System and method for automated nucleic acid amplification
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
AU2014312043A1 (en) 2013-08-30 2016-02-25 Illumina France Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US10294511B2 (en) 2013-10-17 2019-05-21 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
CA2931533C (en) 2013-12-09 2023-08-08 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
DE202015009494U1 (en) 2014-04-10 2018-02-08 10X Genomics, Inc. Fluidic devices and systems for encapsulating and partitioning reagents, and their applications
KR20170023979A (en) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. Processes and systems for nucleic acid sequence assembly
MX2016016902A (en) 2014-06-26 2017-03-27 10X Genomics Inc Methods of analyzing nucleic acids from individual cells or cell populations.
EP3183577B1 (en) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible surface functionalization
ES2727656T3 (en) 2014-09-15 2019-10-17 Abvitro Llc High performance sequencing of nucleotide banks
EP3194627B1 (en) 2014-09-18 2023-08-16 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
CN107278234A (en) 2014-10-03 2017-10-20 生命科技公司 Gene order verification composition, method and kit
CN107002128A (en) 2014-10-29 2017-08-01 10X 基因组学有限公司 The method and composition being sequenced for target nucleic acid
ES2772127T3 (en) 2014-10-31 2020-07-07 Illumina Cambridge Ltd DNA copolymer polymers and coatings
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
DK3215616T3 (en) 2014-11-05 2020-03-02 Illumina Cambridge Ltd Reducing DNA damage during sample preparation and sequencing using siderophore chelators
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
SG11201705425SA (en) 2015-01-13 2017-08-30 10X Genomics Inc Systems and methods for visualizing structural variation and phasing information
MX2017010142A (en) 2015-02-09 2017-12-11 10X Genomics Inc Systems and methods for determining structural variation and phasing using variant call data.
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP3271477B1 (en) 2015-03-20 2020-06-24 Novozymes A/S Droplet-based selection by injection
EP3530752B1 (en) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
ES2826880T3 (en) 2015-05-11 2021-05-19 Illumina Inc Platform for the discovery and analysis of therapeutic agents
AU2016263457B2 (en) 2015-05-18 2020-08-27 Saga Diagnostics Ab Detection of target nucleic acid and variants
EP3653728B1 (en) 2015-06-09 2023-02-01 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
JP6612539B2 (en) 2015-06-30 2019-11-27 シスメックス株式会社 Sample processing equipment for genetic testing
CN107924121B (en) 2015-07-07 2021-06-08 亿明达股份有限公司 Selective surface patterning via nanoimprinting
CN107835857A (en) 2015-07-17 2018-03-23 亿明达股份有限公司 For the polymer sheet layer of application to be sequenced
JP6712606B2 (en) 2015-07-30 2020-06-24 イラミーナ インコーポレーテッド Orthogonal deblocking of nucleotides
CN116338219A (en) 2015-08-24 2023-06-27 亿明达股份有限公司 In-line accumulator and flow control system for biological and chemical assays
US10906044B2 (en) 2015-09-02 2021-02-02 Illumina Cambridge Limited Methods of improving droplet operations in fluidic systems with a filler fluid including a surface regenerative silane
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
WO2017053903A1 (en) 2015-09-24 2017-03-30 Abvitro Llc Single amplicon activated exclusion pcr
KR20180085717A (en) 2015-09-24 2018-07-27 에이비비트로, 엘엘씨 Affinity-oligonucleotide conjugates and their use
WO2018057051A1 (en) 2016-09-24 2018-03-29 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
CN113774495A (en) 2015-09-25 2021-12-10 阿布维特罗有限责任公司 High throughput method for T cell receptor targeted identification of naturally paired T cell receptor sequences
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
DK3882357T3 (en) 2015-12-04 2022-08-29 10X Genomics Inc Methods and compositions for the analysis of nucleic acids
WO2017123533A1 (en) 2016-01-11 2017-07-20 Illumina, Inc. Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
JP6735348B2 (en) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド Systems, methods and media for de novo assembly of whole genome sequence data
WO2017172798A1 (en) 2016-03-28 2017-10-05 Illumina, Inc. Multi-plane microarrays
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
CN109313396B (en) 2016-05-18 2022-11-22 Illumina公司 Self-assembly patterning using patterned hydrophobic surfaces
WO2017214561A1 (en) 2016-06-10 2017-12-14 Life Technologies Corporation Methods and compositions for nucleic acid amplification
EP3693458A1 (en) 2016-07-01 2020-08-12 Carlsberg A/S Method of identifying a sub-pool comprising a mutant organism
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc Cartridge assembly
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
CN106967740B (en) * 2017-02-17 2020-11-13 芜湖天明生物技术有限公司 Escherichia coli fusion expression plectasin, preparation method and application thereof
EP3838268B1 (en) * 2017-02-24 2023-05-10 The Regents of the University of California Particle-drop structures and methods for making and using the same
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CN110870018A (en) 2017-05-19 2020-03-06 10X基因组学有限公司 System and method for analyzing a data set
CN109526228B (en) 2017-05-26 2022-11-25 10X基因组学有限公司 Single cell analysis of transposase accessible chromatin
WO2018218222A1 (en) 2017-05-26 2018-11-29 Goldfless Stephen Jacob High-throughput polynucleotide library sequencing and transcriptome analysis
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
JP2018203724A (en) * 2017-05-31 2018-12-27 キヤノン株式会社 Oily composition, and analysis method using the same
WO2018232282A1 (en) * 2017-06-15 2018-12-20 Board Of Regents, The University Of Texas System Methods and platform for screening and selecting metabolites and their receptors
EP3668651B1 (en) 2017-08-15 2024-05-01 Pacific Biosciences of California, Inc. Methods using a scanning apparatus for sequencing nucleic acids
WO2019051335A1 (en) 2017-09-07 2019-03-14 Juno Therapeutics, Inc. Methods of identifying cellular attributes related to outcomes associated with cell therapy
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019076955A1 (en) 2017-10-18 2019-04-25 Novozymes A/S Immunoassay for bio-active polypeptide stability
WO2019079593A1 (en) 2017-10-19 2019-04-25 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019143622A1 (en) 2018-01-19 2019-07-25 Bio-Rad Laboratories, Inc. Mutant dna polymerases
WO2019144905A1 (en) 2018-01-24 2019-08-01 北京光阱管理咨询合伙企业（有限合伙） Microdroplet container, method for preparing microdroplet container, microdroplet spreading method, microdroplet formation kit, temperature control device, oil phase composition for microdroplet formation and treatment method therefor
CA3188454A1 (en) 2018-01-24 2019-08-01 Sniper (Suzhou) Life Technology Co., Ltd Motion controlling mechanism, liquid discharging nozzle, microdroplet generating device and method, liquid driving mechanism and method, microdroplet generating method, and surface processing method of liquid discharging nozzle
CN110064444A (en) * 2018-01-24 2019-07-30 思纳福(北京)医疗科技有限公司 Microlayer model, which generates, uses oil phase composition and its processing method
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019203986A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
JP2021521820A (en) 2018-04-26 2021-08-30 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． Methods and Compositions for Stabilizing Nucleic Acid-Nucleotide-Polymerase Complex
EP3802874A1 (en) 2018-05-31 2021-04-14 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
AU2019312152A1 (en) 2018-07-24 2021-02-18 Pacific Biosciences Of California, Inc. Serial formation of ternary complex species
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US20240011086A1 (en) 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
CN211311477U (en) 2018-12-04 2020-08-21 欧姆尼欧美公司 Sequencing system
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
EP3894587A1 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Resolving spatial arrays by proximity-based deconvolution
WO2020132103A1 (en) 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN113613787B (en) 2019-02-20 2023-06-13 加利福尼亚太平洋生物科学股份有限公司 Scanning device and method for detecting chemical and biological analytes
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
EP4028407B1 (en) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible modification of nucleotides
IL292003A (en) 2019-10-10 2022-06-01 Carlsberg As Methods for preparing mutant plants
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US20230054204A1 (en) 2020-02-04 2023-02-23 Pacific Biosciences Of California, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4146822A1 (en) 2020-05-05 2023-03-15 Pacific Biosciences of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
CN116034166A (en) 2020-06-25 2023-04-28 10X基因组学有限公司 Spatial analysis of DNA methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
CN112250739B (en) * 2020-09-25 2022-04-08 广东海纳川生物科技股份有限公司 Myceliophthora additive and application thereof
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022155331A1 (en) 2021-01-13 2022-07-21 Pacific Biosciences Of California, Inc. Surface structuring with colloidal assembly
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN117222747A (en) 2021-03-22 2023-12-12 伊鲁米纳剑桥有限公司 Methods for improving clonality of nucleic acid clusters
CN117677435A (en) 2021-06-15 2024-03-08 伊鲁米纳公司 Surface functionalization of non-aqueous gels for sequencing
KR20240031968A (en) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 Intensity extraction with interpolation and adaptation to base calling
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
CN117813391A (en) 2021-07-23 2024-04-02 因美纳有限公司 Method for preparing substrate surface for DNA sequencing
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023049212A2 (en) 2021-09-22 2023-03-30 Illumina, Inc. State-based base calling
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023067110A1 (en) 2021-10-20 2023-04-27 Tataa Biocenter Ab Methods and compositions for detection of mutant nucleic acid sequences
AU2023208743A1 (en) 2022-01-20 2024-01-04 Illumina, Inc. Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
WO2023170272A1 (en) 2022-03-11 2023-09-14 Carlsberg A/S Modulation of protein levels
US20230343414A1 (en) 2022-03-25 2023-10-26 Illumina, Inc. Sequence-to-sequence base calling
WO2023239917A1 (en) 2022-06-09 2023-12-14 Illumina, Inc. Dependence of base calling on flow cell tilt

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0579347A1 (en) * 1992-07-10 1994-01-19 FERRING ARZNEIMITTEL GmbH Microencapsulation of water-soluble drugs
WO1994023738A1 (en) * 1993-04-19 1994-10-27 Medisorb Technologies International L.P. Encapsulation of nucleic acids with conjugates that facilitate and target cellular uptake and gene expression
WO1995024929A2 (en) * 1994-03-15 1995-09-21 Brown University Research Foundation Polymeric gene delivery system
WO1999002671A1 (en) * 1997-07-07 1999-01-21 Medical Research Council In vitro sorting method

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4801529A (en) * 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
US4989892A (en) * 1987-01-23 1991-02-05 Kerins George C Trailer hitch
WO1991005058A1 (en) 1989-10-05 1991-04-18 Glenn Kawasaki Cell-free synthesis and isolation of novel genes and polypeptides
GB9107628D0 (en) 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
JPH06509473A (en) 1991-08-10 1994-10-27 メディカル・リサーチ・カウンシル Processing of cell populations
US5516635A (en) * 1991-10-15 1996-05-14 Ekins; Roger P. Binding assay employing labelled reagent
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5413924A (en) * 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
IL104384A (en) 1993-01-13 1996-11-14 Yeda Res & Dev Method for screening catalytic non-enzyme polypeptides and proteins
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
EP0695368A4 (en) 1993-04-19 1996-05-08 Stuart A Kauffman Random chemistry for the generation of new compounds
AU8124694A (en) 1993-10-29 1995-05-22 Affymax Technologies N.V. In vitro peptide and antibody display libraries
JPH11504218A (en) 1995-04-24 1999-04-20 クロマゾーム コーポレーション Methods for generating and screening new metabolic pathways
US5910408A (en) 1995-06-07 1999-06-08 The General Hospital Corporation Catalytic DNA having ligase activity
EP0748860B1 (en) 1995-06-14 2001-08-29 Tonen Corporation Demulsification by microorganisms
DE19646372C1 (en) 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Conjugates of polypeptide and encoding nucleic acid
GB9608540D0 (en) * 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US6083693A (en) 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
AU4596597A (en) 1996-09-27 1998-04-17 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US6310354B1 (en) * 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
DK0971946T3 (en) 1997-01-21 2006-10-30 Gen Hospital Corp Selection of proteins using RNA-protein fusions
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6023540A (en) * 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
EP0975969A4 (en) 1997-03-18 2002-07-31 Chromaxome Corp Methods for screening compounds using encapsulated cells
AU1409699A (en) 1997-11-13 1999-06-07 Mayo Foundation For Medical Education And Research Detection of pr3-specific anti-neutrophil cytoplasmic autoantibodies in human serum
JP2981547B1 (en) * 1998-07-02 1999-11-22 農林水産省食品総合研究所長 Cross-flow type microchannel device and method for producing or separating emulsion using the device
EP1100889A4 (en) 1998-07-17 2002-03-06 Mirus Corp Micellar systems
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
JP3762224B2 (en) * 1999-04-07 2006-04-05 株式会社東芝 Storage medium for digital information including audio information, recording method and reproducing method using the medium, and recording apparatus and reproducing apparatus using the medium
GB9921155D0 (en) 1999-09-08 1999-11-10 Medical Res Council Selection system
CN1189159C (en) * 2000-05-05 2005-02-16 欧莱雅 Micro-capsule contg. water soluble beauty-care activity component water nuclear, and composition contg. same
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US6936264B2 (en) * 2001-03-05 2005-08-30 The Procter & Gamble Company Delivery of reactive agents via multiple emulsions for use in shelf stable products
GB0114856D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selection by avidity capture
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
JP4395133B2 (en) 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Single molecule amplification and detection of DNA
ES2342665T3 (en) 2003-01-29 2010-07-12 454 Corporation SEQUENCING FROM TWO EXTREME.

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0579347A1 (en) * 1992-07-10 1994-01-19 FERRING ARZNEIMITTEL GmbH Microencapsulation of water-soluble drugs
WO1994023738A1 (en) * 1993-04-19 1994-10-27 Medisorb Technologies International L.P. Encapsulation of nucleic acids with conjugates that facilitate and target cellular uptake and gene expression
WO1995024929A2 (en) * 1994-03-15 1995-09-21 Brown University Research Foundation Polymeric gene delivery system
US20010020011A1 (en) * 1994-03-15 2001-09-06 Edith Mathiowitz Polymeric gene delivery system
WO1999002671A1 (en) * 1997-07-07 1999-01-21 Medical Research Council In vitro sorting method

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9448172B2 (en) 2003-03-31 2016-09-20 Medical Research Council Selection by compartmentalised screening
US8871444B2 (en) 2004-10-08 2014-10-28 Medical Research Council In vitro evolution in microfluidic systems
US9186643B2 (en) 2004-10-08 2015-11-17 Medical Research Council In vitro evolution in microfluidic systems
US9498759B2 (en) 2004-10-12 2016-11-22 President And Fellows Of Harvard College Compartmentalized screening by microfluidic control
US9534216B2 (en) 2006-01-11 2017-01-03 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9328344B2 (en) 2006-01-11 2016-05-03 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9410151B2 (en) 2006-01-11 2016-08-09 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US9273308B2 (en) 2006-05-11 2016-03-01 Raindance Technologies, Inc. Selection of compartmentalized screening method
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US9498761B2 (en) 2006-08-07 2016-11-22 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US9440232B2 (en) 2007-02-06 2016-09-13 Raindance Technologies, Inc. Manipulation of fluids and reactions in microfluidic systems
US9228229B2 (en) 2010-02-12 2016-01-05 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US11965877B2 (en) 2011-02-18 2024-04-23 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing

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