WO2003035829A3 - Nanoparticles having oligonucleotides attached thereto and uses therefor - Google Patents

Nanoparticles having oligonucleotides attached thereto and uses therefor Download PDF

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Publication number
WO2003035829A3
WO2003035829A3 PCT/US2002/032088 US0232088W WO03035829A3 WO 2003035829 A3 WO2003035829 A3 WO 2003035829A3 US 0232088 W US0232088 W US 0232088W WO 03035829 A3 WO03035829 A3 WO 03035829A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
methods
nucleic acid
provides
oligonucleotides
Prior art date
Application number
PCT/US2002/032088
Other languages
French (fr)
Other versions
WO2003035829A2 (en
Inventor
So-Jung Park
Thomas A Taton
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Viswanadham Garimella
Zhi Li
Gang Lu
Original Assignee
Nanosphere Inc
So-Jung Park
Thomas A Taton
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Viswanadham Garimella
Zhi Li
Gang Lu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/008,978 external-priority patent/US6984491B2/en
Application filed by Nanosphere Inc, So-Jung Park, Thomas A Taton, Chad A Mirkin, Robert L Letsinger, Robert C Mucic, James J Storhoff, Robert Elghanian, Viswanadham Garimella, Zhi Li, Gang Lu filed Critical Nanosphere Inc
Priority to JP2003538330A priority Critical patent/JP4347049B2/en
Priority to EP02799155A priority patent/EP1478774A4/en
Priority to AU2002363062A priority patent/AU2002363062B2/en
Priority to CA2463323A priority patent/CA2463323C/en
Publication of WO2003035829A2 publication Critical patent/WO2003035829A2/en
Publication of WO2003035829A3 publication Critical patent/WO2003035829A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Abstract

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
PCT/US2002/032088 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor WO2003035829A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003538330A JP4347049B2 (en) 2001-10-09 2002-10-08 Nanoparticles with oligonucleotides attached and methods of use
EP02799155A EP1478774A4 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor
AU2002363062A AU2002363062B2 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor
CA2463323A CA2463323C (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32786401P 2001-10-09 2001-10-09
US60/327,864 2001-10-09
US10/008,978 US6984491B2 (en) 1996-07-29 2001-12-07 Nanoparticles having oligonucleotides attached thereto and uses therefor
US10/008,978 2001-12-07

Publications (2)

Publication Number Publication Date
WO2003035829A2 WO2003035829A2 (en) 2003-05-01
WO2003035829A3 true WO2003035829A3 (en) 2004-08-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/032088 WO2003035829A2 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor

Country Status (5)

Country Link
EP (1) EP1478774A4 (en)
JP (1) JP4347049B2 (en)
AU (1) AU2002363062B2 (en)
CA (1) CA2463323C (en)
WO (1) WO2003035829A2 (en)

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US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US8323888B2 (en) 1996-07-29 2012-12-04 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries
US11970737B2 (en) 2019-08-26 2024-04-30 Becton, Dickinson And Company Digital counting of individual molecules by stochastic attachment of diverse labels

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US7396677B2 (en) * 2003-11-07 2008-07-08 Nanosphere, Inc. Method of preparing nucleic acids for detection
JP4619012B2 (en) * 2004-02-19 2011-01-26 トヨタ自動車株式会社 Construction method of 3D DNA network
ES2242528B1 (en) 2004-03-25 2006-12-01 Consejo Sup. Investig. Cientificas MAGNETIC NANOPARTICLES OF NOBLE METALS.
MX300005B (en) * 2004-04-23 2012-06-07 Oxonica Inc Surface enhanced spectroscopy-active composite nanoparticles.
JP5398982B2 (en) * 2004-05-24 2014-01-29 ミダテック リミテッド Nanoparticles containing RNA ligands
JP4822753B2 (en) * 2005-07-11 2011-11-24 一般社団法人オンチップ・セロミクス・コンソーシアム Cell component sorting chip, cell component analysis system, and cell component analysis method using them
KR100816419B1 (en) * 2005-10-14 2008-03-27 래플진(주) Method for Isothermal Amplification of Nucleic Acids and Method for Detecting Nucleic Acids Using Simultaneous Isothermal Amplification of Nucleic Acids and Signal Probe
EP1801593A1 (en) * 2005-12-22 2007-06-27 Koninklijke Philips Electronics N.V. A method of imaging biological specimens using inorganic nanoparticles as label agents
EP1981994B1 (en) * 2006-02-08 2014-06-04 Becton, Dickinson and Company Sers nanotag assays
JP5391744B2 (en) * 2009-03-09 2014-01-15 住友ベークライト株式会社 Array substrate
JP5200069B2 (en) * 2010-07-27 2013-05-15 トヨタ自動車株式会社 3D DNA network
KR101792262B1 (en) * 2011-10-10 2017-11-02 삼성전기주식회사 Conductive paste, fabricating method thereof and multi-layer ceramic electronic parts fabricated by using the same
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
JP6375230B2 (en) 2012-02-27 2018-08-15 セルラー リサーチ， インコーポレイテッド Compositions and kits for molecular counting
KR20230074639A (en) 2013-08-28 2023-05-30 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
CN106062210A (en) 2013-12-23 2016-10-26 昆士兰大学 Nucleic acid detection method and kit
JPWO2015159535A1 (en) * 2014-04-16 2017-04-13 富士フイルム株式会社 Detection solution and its preparation method, biosensing method
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
US11535882B2 (en) 2015-03-30 2022-12-27 Becton, Dickinson And Company Methods and compositions for combinatorial barcoding
CN107580632B (en) 2015-04-23 2021-12-28 贝克顿迪金森公司 Methods and compositions for whole transcriptome amplification
CN107636162B (en) 2015-06-01 2021-12-10 加利福尼亚技术学院 Compositions and methods for screening T cells with antigens directed to specific populations
JP6940484B2 (en) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド Methods and compositions for library normalization
AU2017268369B2 (en) * 2016-05-18 2022-09-01 Integrated Nano-Technologies, Inc. Method for detection of a PCR product
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
KR102363716B1 (en) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. Determination of protein expression using reagents having barcoded oligonucleotide sequences
WO2018119128A1 (en) * 2016-12-22 2018-06-28 Burris Robert Barton Methods for non-enzymatic amplification of a signal and uses thereof to detect and quantify a target analyte
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US10852274B2 (en) 2017-03-09 2020-12-01 Auburn University Differential circuit for background correction in electrochemical measurements
EP4345172A2 (en) 2017-06-05 2024-04-03 Becton, Dickinson and Company Sample indexing for single cells
US11505799B2 (en) 2017-07-07 2022-11-22 Innamed, Inc. Aptamers for measuring lipoprotein levels
KR20190011117A (en) * 2017-07-24 2019-02-01 사회복지법인 삼성생명공익재단 Method for detection of genetic mutation by using aggregation of gold nanoparticles
CN112272710A (en) 2018-05-03 2021-01-26 贝克顿迪金森公司 High throughput omics sample analysis
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
WO2020014270A1 (en) * 2018-07-10 2020-01-16 The Regents Of The University Of California Biomolecule coated particles and films and uses thereof
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
CN114729350A (en) 2019-11-08 2022-07-08 贝克顿迪金森公司 Obtaining full-length V (D) J information for immunohistorian sequencing using random priming
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN113088517B (en) * 2021-03-15 2023-10-03 华南师范大学 Construction method of nano probe
CN115178746B (en) * 2022-07-13 2024-03-01 宁波大学 Preparation method and application of gold nanoparticles for detecting mercury ions in cosmetic water
CN115369153B (en) * 2022-10-25 2023-03-10 中国医学科学院北京协和医院 Smad4 gene detection biosensor and preparation method thereof

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Patent Citations (2)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8323888B2 (en) 1996-07-29 2012-12-04 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries
US11970737B2 (en) 2019-08-26 2024-04-30 Becton, Dickinson And Company Digital counting of individual molecules by stochastic attachment of diverse labels

Also Published As

Publication number Publication date
JP4347049B2 (en) 2009-10-21
CA2463323C (en) 2010-12-21
CA2463323A1 (en) 2003-05-01
AU2002363062B2 (en) 2007-03-22
EP1478774A2 (en) 2004-11-24
WO2003035829A2 (en) 2003-05-01
EP1478774A4 (en) 2006-11-29
JP2005525084A (en) 2005-08-25

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