WO2003004686A3 - Fluorescence-based assay for the detection of specific nucleic acids using photon counting - Google Patents

Fluorescence-based assay for the detection of specific nucleic acids using photon counting Download PDF

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Publication number
WO2003004686A3
WO2003004686A3 PCT/US2002/021353 US0221353W WO03004686A3 WO 2003004686 A3 WO2003004686 A3 WO 2003004686A3 US 0221353 W US0221353 W US 0221353W WO 03004686 A3 WO03004686 A3 WO 03004686A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
nucleic acids
fluorescent
labeled
assay
Prior art date
Application number
PCT/US2002/021353
Other languages
French (fr)
Other versions
WO2003004686A2 (en
Inventor
Robert M Dowben
Alford L Marlin
Original Assignee
Advanced Dna Technologies Inc
Robert M Dowben
Alford L Marlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Dna Technologies Inc, Robert M Dowben, Alford L Marlin filed Critical Advanced Dna Technologies Inc
Priority to AU2002354805A priority Critical patent/AU2002354805A1/en
Publication of WO2003004686A2 publication Critical patent/WO2003004686A2/en
Publication of WO2003004686A3 publication Critical patent/WO2003004686A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Abstract

The present assay is directed to a sensitive assay for detecting the presence of small amounts of a specific nThe present assay is directed to a sensitive assay for detecting the presence of small amounts of a specific nucleic acid sequence or sequences in a mixture of sample nucleic acids using photon counting, wherein the sample nucleic acids have not been amplified by polymerase chain reaction prior to assay or further separated from the mixture of sample nucleic acids once the mixture has been isolated from its original source. In one aspect, the present assay comprises binding a mixture of sample nucleic acids to a solid matrix; contacting the matrix-bound sample nucleic acids with a fluorescent-labeled nucleic acid probe; washing the matrix-bound sample nucleic acids under stringent conditions to remove any free or non-specifically bound fluorescent-labeled nucleic acid probe; removing the specifically bound fluorescent-labeled nucleic acid probe; and detecting the presence of the specifically bound fluorescent-labeled nucleic acid probe using photon counting, wherein the presence of fluorescent-labeled probe is indicative of the presence of a target nucleic acid or nucleic acids in the biological sample. In another aspect, the assay includes a polypeptide-tagged nucleic acid probe and a fluorescent-labeled antibody that is specific for the polypeptide tag of the nucleic acid probe, wherein the fluorescent-labeled antibody is detected using photon counting and indicative of the presence of a target nucleic acid or nucleic acids in the biological sample.
PCT/US2002/021353 2001-07-03 2002-07-03 Fluorescence-based assay for the detection of specific nucleic acids using photon counting WO2003004686A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002354805A AU2002354805A1 (en) 2001-07-03 2002-07-03 Fluorescence-based assay for the detection of specific nucleic acids using photon counting

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30289601P 2001-07-03 2001-07-03
US60/302,896 2001-07-03

Publications (2)

Publication Number Publication Date
WO2003004686A2 WO2003004686A2 (en) 2003-01-16
WO2003004686A3 true WO2003004686A3 (en) 2003-08-21

Family

ID=23169671

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/021353 WO2003004686A2 (en) 2001-07-03 2002-07-03 Fluorescence-based assay for the detection of specific nucleic acids using photon counting

Country Status (2)

Country Link
AU (1) AU2002354805A1 (en)
WO (1) WO2003004686A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080044919A1 (en) * 2004-08-13 2008-02-21 Dowben Robert M Analyte Detection Using Time-Resolved Photon Counting Fluorescence
WO2017126479A1 (en) * 2016-01-22 2017-07-27 アークレイ株式会社 Target analysis method and target analysis chip

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4824776A (en) * 1985-07-25 1989-04-25 Molecular Biosystems, Inc. Method for increasing the sensitivity of nucleic acid hybridization assays
US5262526A (en) * 1990-12-21 1993-11-16 Dojindo Laboratories Fluorescent compound, complex, reagent, and specific binding assay employing said reagent
US5714331A (en) * 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5914228A (en) * 1991-09-12 1999-06-22 Cedars-Sinai Medical Center Direct detection of hepatitis C virus RNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4824776A (en) * 1985-07-25 1989-04-25 Molecular Biosystems, Inc. Method for increasing the sensitivity of nucleic acid hybridization assays
US5262526A (en) * 1990-12-21 1993-11-16 Dojindo Laboratories Fluorescent compound, complex, reagent, and specific binding assay employing said reagent
US5714331A (en) * 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5914228A (en) * 1991-09-12 1999-06-22 Cedars-Sinai Medical Center Direct detection of hepatitis C virus RNA

Also Published As

Publication number Publication date
WO2003004686A2 (en) 2003-01-16
AU2002354805A1 (en) 2003-01-21

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