WO2002101358A3 - Multiplexed detection methods - Google Patents

Multiplexed detection methods Download PDF

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Publication number
WO2002101358A3
WO2002101358A3 PCT/US2002/018634 US0218634W WO02101358A3 WO 2002101358 A3 WO2002101358 A3 WO 2002101358A3 US 0218634 W US0218634 W US 0218634W WO 02101358 A3 WO02101358 A3 WO 02101358A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
probes
multiplexed detection
method includes
target molecules
Prior art date
Application number
PCT/US2002/018634
Other languages
French (fr)
Other versions
WO2002101358A9 (en
WO2002101358A2 (en
Inventor
Jian-Bing Fan
John R Stuelpnagel
Mark S Chee
Original Assignee
Illumina Inc
Jian-Bing Fan
John R Stuelpnagel
Mark S Chee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/915,231 external-priority patent/US6890741B2/en
Application filed by Illumina Inc, Jian-Bing Fan, John R Stuelpnagel, Mark S Chee filed Critical Illumina Inc
Priority to EP02744306A priority Critical patent/EP1395805A4/en
Priority to CA002450139A priority patent/CA2450139A1/en
Publication of WO2002101358A2 publication Critical patent/WO2002101358A2/en
Publication of WO2002101358A9 publication Critical patent/WO2002101358A9/en
Publication of WO2002101358A3 publication Critical patent/WO2002101358A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Abstract

The present invention permits highly multiplexed detection of target molecules in a sample. The method includes contacting target analytes with a composition comprising an amplification enzyme and first and second target probes. The first and second target probes comprise first and second bioactive agent respectively, that specifically bind to the first and second target molecules. The probes may also comprise first and second adapter sequences, respectively, such and that the first adapter sequence identifies the first target molecule and the second adapter sequence identifies the second target molecule, and first and section upstream universal priming sequences, respectively. Finally, following amplification the method includes detecting the first and second amplicons, whereby the first and second target molecules, respectively are detected.
PCT/US2002/018634 2001-06-11 2002-06-11 Multiplexed detection methods WO2002101358A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02744306A EP1395805A4 (en) 2001-06-11 2002-06-11 Multiplexed detection methods
CA002450139A CA2450139A1 (en) 2001-06-11 2002-06-11 Multiplexed detection methods

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US29760901P 2001-06-11 2001-06-11
US60/297,609 2001-06-11
US09/915,231 US6890741B2 (en) 2000-02-07 2001-07-24 Multiplexed detection of analytes
US09/915,231 2001-07-24
US31127101P 2001-08-09 2001-08-09
US60/311,271 2001-08-09

Publications (3)

Publication Number Publication Date
WO2002101358A2 WO2002101358A2 (en) 2002-12-19
WO2002101358A9 WO2002101358A9 (en) 2003-03-27
WO2002101358A3 true WO2002101358A3 (en) 2003-12-11

Family

ID=27404500

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/018634 WO2002101358A2 (en) 2001-06-11 2002-06-11 Multiplexed detection methods

Country Status (3)

Country Link
EP (1) EP1395805A4 (en)
CA (1) CA2450139A1 (en)
WO (1) WO2002101358A2 (en)

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US7241629B2 (en) 2001-12-20 2007-07-10 Corning Incorporated Detectable labels, methods of manufacture and use
US7824856B2 (en) 2003-09-10 2010-11-02 Althea Technologies, Inc. Expression profiling using microarrays
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
CN107208158B (en) 2015-02-27 2022-01-28 贝克顿迪金森公司 Spatially addressable molecular barcode
EP3835431B1 (en) 2015-03-30 2022-11-02 Becton, Dickinson and Company Methods for combinatorial barcoding
EP3286326A1 (en) 2015-04-23 2018-02-28 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
JP6940484B2 (en) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド Methods and compositions for library normalization
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP3472359B1 (en) 2016-06-21 2022-03-16 10X Genomics, Inc. Nucleic acid sequencing
CN109791157B (en) 2016-09-26 2022-06-07 贝克顿迪金森公司 Measuring protein expression using reagents with barcoded oligonucleotide sequences
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
CA3103719A1 (en) 2018-10-31 2020-05-07 Illumina, Inc. Polymerases, compositions, and methods of use
JP2022506546A (en) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー Single-cell whole transcriptome analysis using random priming
CA3103739A1 (en) 2018-12-05 2020-06-11 Illumina, Inc. Polymerases, compositions, and methods of use
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
ES2945227T3 (en) 2019-01-23 2023-06-29 Becton Dickinson Co Antibody Associated Oligonucleotides
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
CN115244184A (en) 2020-01-13 2022-10-25 贝克顿迪金森公司 Methods and compositions for quantifying protein and RNA
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins

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CA2370976C (en) * 1999-04-20 2009-10-20 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
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Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027889A (en) * 1996-05-29 2000-02-22 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1395805A4 *

Also Published As

Publication number Publication date
WO2002101358A9 (en) 2003-03-27
WO2002101358A2 (en) 2002-12-19
CA2450139A1 (en) 2002-12-19
EP1395805A4 (en) 2005-03-09
EP1395805A2 (en) 2004-03-10

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