WO2002000938A3 - Methods and compositions for transcription-based nucleic acid amplification - Google Patents

Methods and compositions for transcription-based nucleic acid amplification Download PDF

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Publication number
WO2002000938A3
WO2002000938A3 PCT/US2001/020660 US0120660W WO0200938A3 WO 2002000938 A3 WO2002000938 A3 WO 2002000938A3 US 0120660 W US0120660 W US 0120660W WO 0200938 A3 WO0200938 A3 WO 0200938A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplification
methods
transcription
module
rna transcripts
Prior art date
Application number
PCT/US2001/020660
Other languages
French (fr)
Other versions
WO2002000938A2 (en
WO2002000938A9 (en
Inventor
Nurith Kurn
Original Assignee
Nugen Technologies Inc
Nurith Kurn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies Inc, Nurith Kurn filed Critical Nugen Technologies Inc
Priority to CA002412721A priority Critical patent/CA2412721A1/en
Priority to MXPA02012735A priority patent/MXPA02012735A/en
Priority to EP01950624A priority patent/EP1356094B1/en
Priority to JP2002506251A priority patent/JP2004513617A/en
Priority to AT01950624T priority patent/ATE455186T1/en
Priority to AU2001271595A priority patent/AU2001271595A1/en
Priority to DE60141087T priority patent/DE60141087D1/en
Publication of WO2002000938A2 publication Critical patent/WO2002000938A2/en
Publication of WO2002000938A9 publication Critical patent/WO2002000938A9/en
Publication of WO2002000938A3 publication Critical patent/WO2002000938A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

Methods for isothermal exponential amplification of a target polynucleotide are disclosed. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. In one aspect, the amplification for the first module is composite primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product comprising a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification results.
PCT/US2001/020660 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification WO2002000938A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA002412721A CA2412721A1 (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification
MXPA02012735A MXPA02012735A (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification.
EP01950624A EP1356094B1 (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification
JP2002506251A JP2004513617A (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification
AT01950624T ATE455186T1 (en) 2000-06-26 2001-06-26 METHODS AND COMPOSITIONS FOR TRANSCRIPTION-BASED DUPLICATION OF NUCLEIC ACIDS
AU2001271595A AU2001271595A1 (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification
DE60141087T DE60141087D1 (en) 2000-06-26 2001-06-26 METHODS AND COMPOSITIONS FOR TRANSCRIPTION-REPRODUCTIVE MACHINING OF NUCLEIC ACIDS

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US21390800P 2000-06-26 2000-06-26
US60/213,908 2000-06-26
US27774801P 2001-03-21 2001-03-21
US60/277,748 2001-03-21

Publications (3)

Publication Number Publication Date
WO2002000938A2 WO2002000938A2 (en) 2002-01-03
WO2002000938A9 WO2002000938A9 (en) 2003-07-03
WO2002000938A3 true WO2002000938A3 (en) 2003-08-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/020660 WO2002000938A2 (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification

Country Status (10)

Country Link
US (3) US6686156B2 (en)
EP (1) EP1356094B1 (en)
JP (1) JP2004513617A (en)
CN (1) CN1498274A (en)
AT (1) ATE455186T1 (en)
AU (1) AU2001271595A1 (en)
CA (1) CA2412721A1 (en)
DE (1) DE60141087D1 (en)
MX (1) MXPA02012735A (en)
WO (1) WO2002000938A2 (en)

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US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US8143001B2 (en) 2003-12-29 2012-03-27 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
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