WO2001083702A3 - Novel members of the lysyl oxidases family of amine oxidases related applications - Google Patents

Novel members of the lysyl oxidases family of amine oxidases related applications Download PDF

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Publication number
WO2001083702A3
WO2001083702A3 PCT/US2001/014472 US0114472W WO0183702A3 WO 2001083702 A3 WO2001083702 A3 WO 2001083702A3 US 0114472 W US0114472 W US 0114472W WO 0183702 A3 WO0183702 A3 WO 0183702A3
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WO
WIPO (PCT)
Prior art keywords
proteins
lox
family
present
antibodies
Prior art date
Application number
PCT/US2001/014472
Other languages
French (fr)
Other versions
WO2001083702A2 (en
Inventor
Katalin Csiszar
Charles D Boyd
Youngho Kim
Saux Claude Jourdan Le
Sheri F T Fong
Original Assignee
Univ Hawaii
Katalin Csiszar
Charles D Boyd
Youngho Kim
Saux Claude Jourdan Le
Sheri F T Fong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Hawaii, Katalin Csiszar, Charles D Boyd, Youngho Kim, Saux Claude Jourdan Le, Sheri F T Fong filed Critical Univ Hawaii
Priority to AU2001261194A priority Critical patent/AU2001261194A1/en
Publication of WO2001083702A2 publication Critical patent/WO2001083702A2/en
Publication of WO2001083702A3 publication Critical patent/WO2001083702A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12N9/0024—D-Amino acid oxidase (1.4.3.3)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out

Abstract

In accordance with the present invention, there are provided additional LOX-like proteins characterized by their structural homology to LOX, LOXL1 and LOXL2, and containing a copper binding domain and a catalytic domain. Nucleic acid sequences encoding LOXL3 and LOXL4 proteins, antibodies derived threfrom, as well as assays employing these proteins for determining tissue distribution and catalytic activites, are also disclosed. Invention proteins can be employed in a variety of ways, such as, for example, in bioassays, for production of antibodies thereto, in therapeutic compositions containing such proteins and/or antibodies. The present invention also discloses methods and diagnostic systems for determining the levels or activities of proteins (or functional fragments thereof) related to the LOX gene family, methods for producing a transgenic animal model comprising disrupted genes encoding proteins (or fragments thereof) related to LOX gene family, and methods for identifying compound(s) which modulate the activity of proteins (or functional fragments thereof) related to the LOX gene family. The present invention therefore, discloses the gene structure, chromosomal localization, protein domain structure, evolutionary relationship, tissue specific expression and distribution of lysyl oxidase (LOX) and LOX-like proteins including but not limited to LOXL, LOXL2, LOXL3 and LOXL4, that defines a family of proteins present in distinct cellular and tissue locations, each with a related but different function.
PCT/US2001/014472 2000-05-03 2001-05-03 Novel members of the lysyl oxidases family of amine oxidases related applications WO2001083702A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001261194A AU2001261194A1 (en) 2000-05-03 2001-05-03 Novel members of the lysyl oxidases family of amine oxidases related applications

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20158700P 2000-05-03 2000-05-03
US60/201,587 2000-05-03

Publications (2)

Publication Number Publication Date
WO2001083702A2 WO2001083702A2 (en) 2001-11-08
WO2001083702A3 true WO2001083702A3 (en) 2005-03-03

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/014472 WO2001083702A2 (en) 2000-05-03 2001-05-03 Novel members of the lysyl oxidases family of amine oxidases related applications

Country Status (2)

Country Link
AU (1) AU2001261194A1 (en)
WO (1) WO2001083702A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8163494B2 (en) 2002-11-27 2012-04-24 Technion Research & Development Foundation Ltd. Method for assessing metastatic properties of breast cancer
US8461303B2 (en) 2007-08-02 2013-06-11 Gilead Biologics, Inc. LOX and LOXL2 inhibitors and uses thereof
US8927700B2 (en) 2009-08-21 2015-01-06 Gilead Biologics, Inc. Catalytic domains from lysyl oxidase and LOXL2
US9107935B2 (en) 2009-01-06 2015-08-18 Gilead Biologics, Inc. Chemotherapeutic methods and compositions

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1315519T3 (en) * 2000-08-08 2011-04-11 Technion Res & Dev Foundation Pharmaceutical compositions and methods that can be used to treat cancer or liver fibrosis
IL154036A0 (en) 2000-08-08 2003-07-31 Wyeth Corp A novel member of the lysyl oxidase gene family
GB2438999B (en) * 2003-06-13 2008-01-16 Engelhard Lyon Stimulation of the synthesis and of the activity of an isoform of lysyl oxidase-like LOXL for stimulating the formation of elastic fibres
FR2855968B1 (en) 2003-06-13 2012-11-30 Coletica STIMULATION OF THE SYNTHESIS AND ACTIVITY OF A LYSYL OXIDASE-LIKE LOXL ISOFORM TO STIMULATE THE FORMATION OF ELASTIC FIBERS
FR2855969B1 (en) * 2003-06-13 2012-11-30 Coletica STIMULATION OF THE ACTIVITY OF A LYSYL OXIDASE ISOFORM TO FIGHT CERTAIN PATHOLOGIES DUE TO AN INCOMPLETE, ABSENT OR DISORGANIZED ELASTOGENESIS
JP2007526247A (en) * 2004-01-23 2007-09-13 マサチューセッツ・アイ・アンド・イア・インファーマリー Lysyl oxidase-like 1 (LOXL1) and elastic fiber formation
FR2892635B1 (en) 2005-10-28 2012-11-09 Engelhard Lyon SUBSTANCE FOR RESTORING NORMAL CO-EXPRESSION AND INTERACTION BETWEEN LOX AND NRAGE PROTEINS
IL184627A0 (en) 2007-07-15 2008-12-29 Technion Res & Dev Foundation Agents for diagnosing and modulating metastasis and fibrosis as well as inflammation in a mammalian tissue
JP6059859B2 (en) * 2009-11-16 2017-01-11 ビーエーエスエフ ビューティ ケア ソリューションズ フランス エスエーエスＢＡＳＦ Ｂｅａｕｔｙ Ｃａｒｅ Ｓｏｌｕｔｉｏｎｓ Ｆｒａｎｃｅ Ｓ．Ａ．Ｓ． Stimulation of synthesis and activity of LOXL (lysyl oxidase-like) isoforms to stimulate the formation of elastic fibers
CN105622757A (en) * 2010-02-04 2016-06-01 吉联亚生物科技有限公司 Antibodies that bind to lysyl oxidase-like 2(LOXL2) and methods of use therefor
EP2694554A1 (en) 2011-04-08 2014-02-12 Gilead Biologics, Inc. Methods and compositions for normalization of tumor vasculature by inhibition of loxl2
EA201692202A1 (en) 2014-06-11 2017-06-30 Джилид Сайэнс, Инк. METHODS OF TREATING CARDIOVASCULAR DISEASES
EP2990480A1 (en) * 2014-08-29 2016-03-02 Christian-Albrechts-Universität zu Kiel Anti-LOXL4 antibodies and derivatives there
JP2015147798A (en) * 2015-04-20 2015-08-20 ビーエーエスエフ ビューティ ケア ソリューションズ フランス エスエーエス Stimulation of synthesis and of activity of isoform of loxl (lysyl oxidase-like) for stimulating formation of elastic fiber

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6140056A (en) * 1999-01-27 2000-10-31 Millennium Pharmaceuticals, Inc. MSP-18 protein and nucleic acid molecules and uses therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6140056A (en) * 1999-01-27 2000-10-31 Millennium Pharmaceuticals, Inc. MSP-18 protein and nucleic acid molecules and uses therefor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ITO H. ET AL.: "Molecular cloning and biological activity of a novel lysyl oxidase gene expressed in cartillage", J. BIOL. CHEM., vol. 276, no. 2, 5 April 2001 (2001-04-05), pages 24023 - 24029, XP002199860 *
MAKI J.M.: "Cloning and characterization of a four human lysyl oxidase isoenzyme", BIOCHEM. J., vol. 355, 15 April 2001 (2001-04-15), pages 381 - 387, XP002983391 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8163494B2 (en) 2002-11-27 2012-04-24 Technion Research & Development Foundation Ltd. Method for assessing metastatic properties of breast cancer
US8461303B2 (en) 2007-08-02 2013-06-11 Gilead Biologics, Inc. LOX and LOXL2 inhibitors and uses thereof
US8679485B2 (en) 2007-08-02 2014-03-25 Gilead Biologics, Inc. Methods and compositions for treatment and diagnosis of fibrosis, tumor invasion, angiogenesis, and metastasis
US9176139B2 (en) 2007-08-02 2015-11-03 Gilead Biologics, Inc. LOX and LOXL2 inhibitors and uses thereof
US9107935B2 (en) 2009-01-06 2015-08-18 Gilead Biologics, Inc. Chemotherapeutic methods and compositions
US9289447B2 (en) 2009-01-06 2016-03-22 Gilead Biologics, Inc. Chemotherapeutic methods and compositions
US8927700B2 (en) 2009-08-21 2015-01-06 Gilead Biologics, Inc. Catalytic domains from lysyl oxidase and LOXL2

Also Published As

Publication number Publication date
AU2001261194A1 (en) 2001-11-12
WO2001083702A2 (en) 2001-11-08

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