WO2001069199A3 - Methods of detecting cancer using cellular autofluorescence - Google Patents

Methods of detecting cancer using cellular autofluorescence Download PDF

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Publication number
WO2001069199A3
WO2001069199A3 PCT/US2001/007589 US0107589W WO0169199A3 WO 2001069199 A3 WO2001069199 A3 WO 2001069199A3 US 0107589 W US0107589 W US 0107589W WO 0169199 A3 WO0169199 A3 WO 0169199A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cells
autofluorescence
tryptophan
detecting cancer
Prior art date
Application number
PCT/US2001/007589
Other languages
French (fr)
Other versions
WO2001069199A2 (en
Inventor
Bhaskar Banerjee
Original Assignee
Bhaskar Banerjee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bhaskar Banerjee filed Critical Bhaskar Banerjee
Priority to US10/221,324 priority Critical patent/US20040038320A1/en
Priority to EP01922318A priority patent/EP1261280A2/en
Priority to CA002402233A priority patent/CA2402233A1/en
Priority to AU2001249134A priority patent/AU2001249134A1/en
Priority to JP2001568033A priority patent/JP2003533674A/en
Publication of WO2001069199A2 publication Critical patent/WO2001069199A2/en
Publication of WO2001069199A3 publication Critical patent/WO2001069199A3/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/42—Detecting, measuring or recording for evaluating the gastrointestinal, the endocrine or the exocrine systems
    • A61B5/4222—Evaluating particular parts, e.g. particular organs
    • A61B5/4233—Evaluating particular parts, e.g. particular organs oesophagus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/43—Detecting, measuring or recording for evaluating the reproductive systems
    • A61B5/4306—Detecting, measuring or recording for evaluating the reproductive systems for evaluating the female reproductive systems, e.g. gynaecological evaluations
    • A61B5/4318—Evaluation of the lower reproductive system
    • A61B5/4331—Evaluation of the lower reproductive system of the cervix
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/43—Detecting, measuring or recording for evaluating the reproductive systems
    • A61B5/4375—Detecting, measuring or recording for evaluating the reproductive systems for evaluating the male reproductive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/44—Detecting, measuring or recording for evaluating the integumentary system, e.g. skin, hair or nails
    • A61B5/441—Skin evaluation, e.g. for skin disorder diagnosis

Abstract

Methods useful in detecting abnormal cells and tissues based upon measurement of tryptophan-associated autofluorescence are disclosed. The methods involve exposing cells to ultraviolet light; measuring autofluorescence at a wavelength indicative of tryptophan emission; and determining that the cells constitute abnormal tissue of emission intensity exceeds that of normal cells. The methods can detect and distinguish abnormal cell development in metaplasia, hyperplasia, dysplasia and cancer. The method also detects the presence of inflammation.
PCT/US2001/007589 2000-03-10 2001-03-09 Methods of detecting cancer using cellular autofluorescence WO2001069199A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/221,324 US20040038320A1 (en) 2001-03-09 2001-03-09 Methods of detecting cancer using cellular autofluorescence
EP01922318A EP1261280A2 (en) 2000-03-10 2001-03-09 Methods of detecting cancer using cellular autofluorescence
CA002402233A CA2402233A1 (en) 2000-03-10 2001-03-09 Methods of detecting cancer using cellular autofluorescence
AU2001249134A AU2001249134A1 (en) 2000-03-10 2001-03-09 Methods of detecting cancer using cellular autofluorescence
JP2001568033A JP2003533674A (en) 2000-03-10 2001-03-09 Method for detecting cancer using cell autofluorescence

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/522,557 US6405070B1 (en) 1998-06-16 2000-03-10 Detection of cancer using cellular autofluorescence
US09/522,557 2000-03-10

Publications (2)

Publication Number Publication Date
WO2001069199A2 WO2001069199A2 (en) 2001-09-20
WO2001069199A3 true WO2001069199A3 (en) 2002-03-28

Family

ID=24081338

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/007589 WO2001069199A2 (en) 2000-03-10 2001-03-09 Methods of detecting cancer using cellular autofluorescence

Country Status (6)

Country Link
US (2) US6405070B1 (en)
EP (1) EP1261280A2 (en)
JP (1) JP2003533674A (en)
AU (1) AU2001249134A1 (en)
CA (1) CA2402233A1 (en)
WO (1) WO2001069199A2 (en)

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Also Published As

Publication number Publication date
AU2001249134A1 (en) 2001-09-24
JP2003533674A (en) 2003-11-11
WO2001069199A2 (en) 2001-09-20
CA2402233A1 (en) 2001-09-20
US20030055341A1 (en) 2003-03-20
US6405070B1 (en) 2002-06-11
EP1261280A2 (en) 2002-12-04

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