WO2001062064A3 - Verfahren zur detektion von cytosin-methylierung in dna proben - Google Patents

Verfahren zur detektion von cytosin-methylierung in dna proben Download PDF

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Publication number
WO2001062064A3
WO2001062064A3 PCT/DE2001/000750 DE0100750W WO0162064A3 WO 2001062064 A3 WO2001062064 A3 WO 2001062064A3 DE 0100750 W DE0100750 W DE 0100750W WO 0162064 A3 WO0162064 A3 WO 0162064A3
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WO
WIPO (PCT)
Prior art keywords
genomic dna
dna samples
cytosine methylation
methylcytosine
amplified
Prior art date
Application number
PCT/DE2001/000750
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English (en)
French (fr)
Other versions
WO2001062064A2 (de
Inventor
Alexander Olek
Kurt Berlin
Original Assignee
Epigenomics Ag
Alexander Olek
Kurt Berlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag, Alexander Olek, Kurt Berlin filed Critical Epigenomics Ag
Priority to AU4228001A priority Critical patent/AU4228001A/xx
Priority to AU2001242280A priority patent/AU2001242280B2/en
Priority to DE50113970T priority patent/DE50113970D1/de
Priority to CA2399281A priority patent/CA2399281C/en
Priority to JP2001561142A priority patent/JP4498657B2/ja
Priority to EP01915054A priority patent/EP1261741B1/de
Priority to US10/220,090 priority patent/US7524629B2/en
Publication of WO2001062064A2 publication Critical patent/WO2001062064A2/de
Publication of WO2001062064A3 publication Critical patent/WO2001062064A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

Beschrieben wird ein Verfahren zum Nachweis von 5-Methylcytosin in genomischen DNA-Proben. Zuerst wird eine genomische DNA aus einer DNA-Probe mit einem Reagenz chemisch umgesetzt, wobei 5-Methylcytosin und Cytosin unterschiedlich reagieren, und anschliessend wird die vorbehandelte DNA unter Verwendung einer Polymerase und mindestens einem Primer amplifiziert. Im nächsten Schritt wird die amplifizierte genomische DNA an mindestens ein Oligonukleotid unter Ausbildung einer Duplex hybridisiert und selbiges um mindestens ein Nukleotid verlängert, wobei das Nukleotid eine nachweisbare Markierung trägt und die Verlängerung vom Methylierungsstatus des jeweiligen Cytosins in der genomischen DNA-Probe abhängt. Im nächsten Schritt werden die verlängerten Oligonukleotide auf das Vorhandensein der Markierung untersucht.
PCT/DE2001/000750 2000-02-25 2001-02-23 Verfahren zur detektion von cytosin-methylierung in dna proben WO2001062064A2 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU4228001A AU4228001A (en) 2000-02-25 2001-02-23 Method for detecting cytosine methylation in dna samples
AU2001242280A AU2001242280B2 (en) 2000-02-25 2001-02-23 Method for detecting cytosine methylation in dna samples
DE50113970T DE50113970D1 (de) 2000-02-25 2001-02-23 Verfahren zur gleichzeitigen detektion von cytosin-methylierung und mutationen in dna proben
CA2399281A CA2399281C (en) 2000-02-25 2001-02-23 Method for detecting cytosine methylation in dna samples
JP2001561142A JP4498657B2 (ja) 2000-02-25 2001-02-23 Ｄｎａサンプル中のシトシン−メチル化とｓｎｐ又は突然変異の同時検出方法
EP01915054A EP1261741B1 (de) 2000-02-25 2001-02-23 Verfahren zur gleichzeitigen detektion von cytosin-methylierung und mutationen in dna proben
US10/220,090 US7524629B2 (en) 2000-02-25 2001-02-23 Method for detecting cytosine methylation in DNA samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10010280A DE10010280B4 (de) 2000-02-25 2000-02-25 Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010280.8 2000-02-25

Publications (2)

Publication Number Publication Date
WO2001062064A2 WO2001062064A2 (de) 2001-08-30
WO2001062064A3 true WO2001062064A3 (de) 2002-10-03

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PCT/DE2001/000750 WO2001062064A2 (de) 2000-02-25 2001-02-23 Verfahren zur detektion von cytosin-methylierung in dna proben

Country Status (9)

Country Link
US (1) US7524629B2 (de)
EP (1) EP1261741B1 (de)
JP (1) JP4498657B2 (de)
AT (1) ATE395434T1 (de)
AU (2) AU2001242280B2 (de)
CA (1) CA2399281C (de)
DE (2) DE10010280B4 (de)
ES (1) ES2307597T3 (de)
WO (1) WO2001062064A2 (de)

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DE10151055B4 (de) 2001-10-05 2005-05-25 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln
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US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
US8129107B2 (en) 2005-04-01 2012-03-06 Epigenomics Ag Bisulfite conversion of DNA
JP5133238B2 (ja) 2005-04-15 2013-01-30 エピゲノミックス アクチェンゲゼルシャフト 遠隔サンプル由来のｄｎａ断片を提供する方法
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP3095878A1 (de) 2005-11-17 2016-11-23 Epigenomics AG Verfahren zur bestimmung des dna-methylierungsgrads einer cpg-position in identischen zellen in einer gewebeprobe
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US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US8084734B2 (en) * 2006-05-26 2011-12-27 The George Washington University Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
EP2152902A2 (de) 2007-06-08 2010-02-17 Epigenomics AG Verfahren zur methylierungsanalyse
EP2808403B1 (de) 2008-08-15 2016-05-25 Cascade Biosystems, Inc. Nukleinsäureerkennung
EP2340314B8 (de) * 2008-10-22 2015-02-18 Illumina, Inc. Erhaltung von informationen in verbindung mit genomischer dna-methylierung
US8110796B2 (en) 2009-01-17 2012-02-07 The George Washington University Nanophotonic production, modulation and switching of ions by silicon microcolumn arrays
US9490113B2 (en) * 2009-04-07 2016-11-08 The George Washington University Tailored nanopost arrays (NAPA) for laser desorption ionization in mass spectrometry
US9624530B2 (en) 2009-08-03 2017-04-18 Epigenomics Ag Methods for preservation of genomic DNA sequence complexity
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
CA2790123A1 (en) 2010-02-15 2011-08-18 Cascade Biosystems, Inc. Methods and materials for detecting viral or microbial infections
EP2536848B1 (de) 2010-02-15 2017-07-19 Cascade Biosystems, Inc. Verfahren und material zur detektion von genetischen und epigenetischen elementen
WO2011100752A2 (en) 2010-02-15 2011-08-18 Cascade Biosystems, Inc. Methods and materials for assessing rna expression
US8623616B2 (en) 2010-02-15 2014-01-07 Cascade Biosystems, Inc. Methods and materials for detecting contaminated food products
WO2013084075A2 (en) * 2011-12-06 2013-06-13 Mdxhealth Sa Methods of detecting mutations and epigenetic changes
US10640808B2 (en) 2013-03-13 2020-05-05 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
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Also Published As

Publication number Publication date
ATE395434T1 (de) 2008-05-15
US7524629B2 (en) 2009-04-28
CA2399281C (en) 2012-01-24
AU2001242280B2 (en) 2007-03-01
JP2003523211A (ja) 2003-08-05
DE10010280B4 (de) 2006-08-10
DE10010280A1 (de) 2001-09-06
EP1261741A2 (de) 2002-12-04
AU4228001A (en) 2001-09-03
US20030129620A1 (en) 2003-07-10
ES2307597T3 (es) 2008-12-01
JP4498657B2 (ja) 2010-07-07
WO2001062064A2 (de) 2001-08-30
DE50113970D1 (de) 2008-06-26
EP1261741B1 (de) 2008-05-14
CA2399281A1 (en) 2001-08-30

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