WO2001027624A3 - Compositions and methods for detecting protein modification and enzymatic activity - Google Patents

Compositions and methods for detecting protein modification and enzymatic activity Download PDF

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Publication number
WO2001027624A3
WO2001027624A3 PCT/US2000/041062 US0041062W WO0127624A3 WO 2001027624 A3 WO2001027624 A3 WO 2001027624A3 US 0041062 W US0041062 W US 0041062W WO 0127624 A3 WO0127624 A3 WO 0127624A3
Authority
WO
WIPO (PCT)
Prior art keywords
modification
protein
protein modification
target protein
detecting
Prior art date
Application number
PCT/US2000/041062
Other languages
French (fr)
Other versions
WO2001027624A2 (en
Inventor
Li Shen
Debra Hui Cen
Original Assignee
Superarray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Superarray Inc filed Critical Superarray Inc
Priority to EP00978895A priority Critical patent/EP1224463A2/en
Priority to AU16305/01A priority patent/AU1630501A/en
Priority to CA002383945A priority patent/CA2383945A1/en
Publication of WO2001027624A2 publication Critical patent/WO2001027624A2/en
Publication of WO2001027624A3 publication Critical patent/WO2001027624A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Abstract

This invention relates generally to the field of protein modification, e.g., post-translational modification. In particular, the invention provides a method for detecting protein modification profile in a sample, which method comprises: a) contacting a sample containing or suspected of containing a target protein with a capture molecule, or a plurality of capture molecules, immobilized on a solid support, said capture molecule is capable of specifically binding to said target protein, whereby said target protein is immobilized on said solid support; and b) assessing modification status and/or identity of said immobilized target protein. Kits and arrays useful for detecting protein modification are also provided. Arrays, kits and methods useful for detecting enzymatic activities, especially protein modification enzymatic activities, are further provided.
PCT/US2000/041062 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity WO2001027624A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00978895A EP1224463A2 (en) 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity
AU16305/01A AU1630501A (en) 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity
CA002383945A CA2383945A1 (en) 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15856099P 1999-10-08 1999-10-08
US60/158,560 1999-10-08

Publications (2)

Publication Number Publication Date
WO2001027624A2 WO2001027624A2 (en) 2001-04-19
WO2001027624A3 true WO2001027624A3 (en) 2002-02-21

Family

ID=22568698

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/041062 WO2001027624A2 (en) 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity

Country Status (5)

Country Link
US (1) US20030153014A1 (en)
EP (1) EP1224463A2 (en)
AU (1) AU1630501A (en)
CA (1) CA2383945A1 (en)
WO (1) WO2001027624A2 (en)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7198896B2 (en) 1998-09-04 2007-04-03 Cell Signaling Technology, Inc. Immunoaffinity isolation of modified peptides from complex mixtures
US20120244594A9 (en) 1998-09-04 2012-09-27 Cell Signaling Technology, Inc. Immunoaffinity isolation of modified peptides from complex mixtures
WO2001036604A2 (en) 1999-11-18 2001-05-25 Corvas International, Inc. Nucleic acids encoding endotheliases, endotheliases and uses thereof
US7700341B2 (en) 2000-02-03 2010-04-20 Dendreon Corporation Nucleic acid molecules encoding transmembrane serine proteases, the encoded proteins and methods based thereon
AU2001256780A1 (en) * 2000-05-18 2001-11-26 Nihon University, School Juridical Person Method for examining ischemic conditions
DE08002964T1 (en) * 2001-02-27 2009-09-03 Blanchette Rockefeller Neurosciences Institute Alzheimer's diagnosis based on mitogen-activated protein kinase phosphorylation
CA2439404A1 (en) * 2001-03-07 2002-09-12 Bio-Rad Laboratories Inc. Assay system for simultaneous detection and measurement of multiple modified cellular proteins
AU2002305052A1 (en) * 2001-03-13 2002-09-24 Corvas International, Inc. Nucleic acid molecules encoding a transmembrane serine protease 7, the encoded polypeptides and methods based thereon
WO2002077263A2 (en) * 2001-03-22 2002-10-03 Dendreon San Diego Llc Nucleic acid molecules encoding serine protease cvsp14, the encoded polypeptides and methods based thereon
NZ527971A (en) 2001-03-27 2006-03-31 Dendreon Corp Nucleic acid molecules encoding a transmembrane serine protease 9, the encoded polypeptides and methods based thereon
US20040253637A1 (en) * 2001-04-13 2004-12-16 Biosite Incorporated Markers for differential diagnosis and methods of use thereof
US20040126767A1 (en) * 2002-12-27 2004-07-01 Biosite Incorporated Method and system for disease detection using marker combinations
US20040121350A1 (en) * 2002-12-24 2004-06-24 Biosite Incorporated System and method for identifying a panel of indicators
US7713705B2 (en) * 2002-12-24 2010-05-11 Biosite, Inc. Markers for differential diagnosis and methods of use thereof
US20040203083A1 (en) * 2001-04-13 2004-10-14 Biosite, Inc. Use of thrombus precursor protein and monocyte chemoattractant protein as diagnostic and prognostic indicators in vascular diseases
KR20040080940A (en) * 2001-05-14 2004-09-20 덴드레온 코포레이션 Nucleic acid molecules encoding a transmembrane serine protease 10, the encoded polypeptides and methods based thereon
CA2454605C (en) * 2001-06-21 2006-11-07 Cell Signaling Technology, Inc. Immunoaffinity isolation of modified peptides from complex mixtures
US20110111424A1 (en) 2001-06-21 2011-05-12 Cell Signaling Technology, Inc. Analysis of ubiquitinated polypeptides
AU2002315525A1 (en) * 2001-07-03 2003-01-21 Dendreon San Diego Llc Nucleic acid molecules encoding a transmembrane serine protease 20, the encoded polypeptides and methods based thereon
DE60231845D1 (en) * 2001-12-11 2009-05-14 Univ New Jersey Med DIAGNOSTIC PROCEDURE FOR PROTEIN PROFILING
AU2003233576A1 (en) * 2002-05-17 2003-12-02 Sugen, Inc. Novel biomarkers of tyrosine kinase inhibitor exposure and activity in mammals
WO2003104394A2 (en) * 2002-05-21 2003-12-18 Dendreon San Diego Llc Nucleic acid molecules encoding a transmembrane serine protease 12, the encoded polypeptides and methods based thereon
WO2004059293A2 (en) * 2002-12-24 2004-07-15 Biosite Incorporated Markers for differential diagnosis and methods of use thereof
US8168568B1 (en) 2003-03-10 2012-05-01 The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Combinatorial therapy for protein signaling diseases
FR2855529A1 (en) * 2003-05-28 2004-12-03 Jean Philippe Goddard Generating a profile of reactivity for a sample, useful e.g. for diagnosis, comprises simultaneous determination of enzymatic activity against many different substrates
US20040265938A1 (en) * 2003-06-25 2004-12-30 Jose Remacle Method for the determination of cell activation
US20050208597A1 (en) * 2004-01-26 2005-09-22 The Board Of Trustees Of The Leland Stanford Junior University Microarray analysis of post-translational modifications
JP2008520203A (en) * 2004-11-15 2008-06-19 ブランシェット・ロックフェラー・ニューロサイエンスィズ・インスティテュート Abnormalities of phosphatase 2A (PP2A) for diagnosis and treatment of Alzheimer's disease
US20090068162A1 (en) * 2004-11-17 2009-03-12 Robert Sack Stationary Phase Antibody Arrays for Trace Protein Analysis
EP1696235B1 (en) * 2005-02-23 2009-10-28 FUJIFILM Corporation Biosensor
US8632983B2 (en) 2005-04-15 2014-01-21 Van Andel Research Institute Biomarkers for pancreatic cancer and diagnostic methods
US7838634B2 (en) * 2005-04-15 2010-11-23 Van Andel Research Institute Methods for measuring glycan levels of proteins
WO2006124644A2 (en) * 2005-05-12 2006-11-23 Board Of Regents, The University Of Texas System Protein and antibody profiling using small molecule microarrays
US7763418B2 (en) * 2005-07-05 2010-07-27 Cytoskeleton, Inc. Detection of Rho proteins
US20080221042A1 (en) * 2005-10-11 2008-09-11 Tapan Kumar Khan Alzheimer's disease-specific alterations of the ERK1/ERK2 Phosphorylation ratio-Alzheimer's disease-specific molecular biomarkers (ADSMB)
US7595167B2 (en) * 2005-10-11 2009-09-29 Blanchette Rockefeller Neurosciences Institute Alzheimer's disease-specific alterations of the Erk1/Erk2 phosphorylation ratio
US20090029873A1 (en) * 2005-10-11 2009-01-29 Blanchette Rockefeller Neurosciences Institute Alzheimer's Disease-Specific Alterations of the Erk1/Erk2 Phosphorylation Ratio-Alzheimer's Disease-Specific Molecular Biomarkers (Adsmb)
CN101421625A (en) * 2005-10-18 2009-04-29 乔治梅森知识产权公司 mTOR pathway theranostic
DE102006001793A1 (en) * 2006-01-12 2007-07-26 Forschungsgesellschaft für Arbeitsphysiologie und Arbeitsschutz e.V. Method and diagnostic kit for the simultaneous identification and quantitation of single or mixed biomolecules and their modifications
US20150232540A1 (en) 2006-07-11 2015-08-20 Cell Signaling Technology, Inc. Analysis of ubiquitnated polypeptides
BRPI0717416A2 (en) 2006-09-21 2013-11-12 Prometheus Lab Inc METHOD FOR PERFORMING A HIGH PRODUCTIVITY COMPLEX IMMUNOASON, AND
EP2179291B1 (en) * 2007-07-13 2014-10-22 Nestec S.A. Drug selection for lung cancer therapy using antibody-based arrays
GB2452076A (en) 2007-08-23 2009-02-25 Mologic Ltd Detection of enzymes by detecting binding of substrate recognition molecules to modified substrates
EP2073012A1 (en) * 2007-12-17 2009-06-24 Fondazione Telethon Method for the isolation and identification of ADP-ribosylated molecules
ES2398618T3 (en) * 2008-02-25 2013-03-20 Nestec S.A. Drug selection for breast cancer therapy using antibody matrices
EP2669386A1 (en) * 2008-07-28 2013-12-04 Blanchette Rockefeller Neurosciences Institute Stimulus-elicited genomic profile markers of alzheimer's disease
EP3403650A3 (en) 2008-07-28 2019-02-13 Blanchette Rockefeller Neurosciences, Institute Pkc-activating compounds for the treatment of neurodegenerative diseases
WO2010044892A1 (en) * 2008-10-17 2010-04-22 President And Fellows Of Harvard College Diagnostic method based on large scale identification of post-translational modification of proteins
US20100303835A1 (en) * 2009-05-29 2010-12-02 The Board Of Regents Of The University Of Texas System Peptoid ligands for isolation and treatment of autoimmune t-cells
JP5828837B2 (en) * 2009-06-02 2015-12-09 ザ ボード オブ リージェンツ オブ ザ ユニバーシティー オブ テキサス システム Identification of small molecules recognized by antibodies in subjects with neurodegenerative diseases
JP5795311B2 (en) 2009-07-15 2015-10-14 ネステク ソシエテ アノニム Drug selection for gastric cancer therapy using antibody-based arrays
JP6058395B2 (en) * 2009-10-02 2017-01-11 ブランシェット・ロックフェラー・ニューロサイエンスィズ・インスティテュート Fibroblast growth patterns for the diagnosis of Alzheimer's disease
BR112012007557A2 (en) * 2009-10-02 2017-05-02 Blanchette Rockefeller Neurosciences Inst abnormal changes in pkc isozyme processing in peripheral cells of alzheimer's disease
US8759259B2 (en) * 2009-10-16 2014-06-24 The Board Of Regents Of The University Of Texas System Compositions and methods for producing cyclic peptoid libraries
US9719995B2 (en) 2011-02-03 2017-08-01 Pierian Holdings, Inc. Drug selection for colorectal cancer therapy using receptor tyrosine kinase profiling
EP2751562B1 (en) 2011-09-02 2015-09-16 Nestec S.A. Profiling of signal pathway proteins to determine therapeutic efficacy
CA2856235A1 (en) 2011-11-13 2013-05-16 Blanchette Rockefeller Neurosciences Institute Esters of dcpla for the treatment of neurodegenerative disorders
US20180371550A1 (en) * 2015-07-08 2018-12-27 Children's Hospital Medical Center Loss of transcriptional fidelity leads to immunotherapy resistance in cancers
US10753936B2 (en) 2016-07-22 2020-08-25 Van Andel Research Institute Method of detecting the level of a glycan
CN107657146B (en) * 2017-09-20 2020-05-05 广州市爱菩新医药科技有限公司 Drug molecule comparison method based on three-dimensional substructure
WO2021144302A1 (en) 2020-01-13 2021-07-22 Inthera Bioscience AG Biomarkers for determining susceptibility to oxopiperazine derivatives and methods of using same
EP4168014A1 (en) 2020-06-19 2023-04-26 Inthera Bioscience AG Oxopiperazine derivatives for the treatment of cancer
CN112415196A (en) * 2020-11-25 2021-02-26 刘世杰 System and application for analyzing molecular activity

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0818467A2 (en) * 1996-07-12 1998-01-14 Nec Corporation Aligned peptide array and a rational and rapid method for the detection of a binding or interaction site of a protein by using the same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136596A (en) * 1995-05-19 2000-10-24 University Of Massachusetts Cytokine-, stress-, and oncoprotein-activated human protein kinase kinases
US5736381A (en) * 1995-05-19 1998-04-07 Davis; Roger J. Cytokine-, stress-, and oncoprotein-activated human protein kinase kinases
US6235873B1 (en) * 1999-07-31 2001-05-22 The Rockefeller University Constitutively active transcription factors and their uses for identifying modulators of activity including dysproliferative cellular changes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0818467A2 (en) * 1996-07-12 1998-01-14 Nec Corporation Aligned peptide array and a rational and rapid method for the detection of a binding or interaction site of a protein by using the same

Also Published As

Publication number Publication date
EP1224463A2 (en) 2002-07-24
CA2383945A1 (en) 2001-04-19
US20030153014A1 (en) 2003-08-14
WO2001027624A2 (en) 2001-04-19
AU1630501A (en) 2001-04-23

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