WO2000071987A3 - Isolation and culturing of fetal cells - Google Patents

Isolation and culturing of fetal cells Download PDF

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Publication number
WO2000071987A3
WO2000071987A3 PCT/DK2000/000281 DK0000281W WO0071987A3 WO 2000071987 A3 WO2000071987 A3 WO 2000071987A3 DK 0000281 W DK0000281 W DK 0000281W WO 0071987 A3 WO0071987 A3 WO 0071987A3
Authority
WO
WIPO (PCT)
Prior art keywords
fetal cells
cells
fetal
labelling
maternal
Prior art date
Application number
PCT/DK2000/000281
Other languages
French (fr)
Other versions
WO2000071987A2 (en
Inventor
John Philip
Britta Christensen
Original Assignee
John Philip
Britta Christensen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by John Philip, Britta Christensen filed Critical John Philip
Priority to AU47452/00A priority Critical patent/AU4745200A/en
Priority to CA002413835A priority patent/CA2413835A1/en
Publication of WO2000071987A2 publication Critical patent/WO2000071987A2/en
Publication of WO2000071987A3 publication Critical patent/WO2000071987A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Wood Science & Technology (AREA)
  • Ecology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to a method for isolating fetal cells from maternal blood, wherein the blood sample has not been substantially enriched, such as by performing, on a sample of maternal blood from which at the most 50 % of the anucleated material cells thereof and/or at the most 50 % of the nucleated maternal cells thereof have been removed, selective labelling of fetal cells in the maternal blood sample, identifying the selectively labelled fetal cells, and specifically isolating substantially only the selectively labelled fetal cells. The selective labelling may be conducted with antibodies specific for the fetal cells and/or probes hybridising for example with fetal mRNA. Also, the invention relates to a method of diagnosing a disease in a fetus wherein the specifically labelling may then be conducted with a marker, such as a probe, to a gene or a gene mutation specific for the genetic disease or a chromosome abnormality to be diagnosed.
PCT/DK2000/000281 1999-05-25 2000-05-25 Isolation and culturing of fetal cells WO2000071987A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU47452/00A AU4745200A (en) 1999-05-25 2000-05-25 Isolation and culturing of fetal cells
CA002413835A CA2413835A1 (en) 1999-05-25 2000-05-25 Isolation and culturing of fetal cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99201651.9 1999-05-25
EP99201651 1999-05-25

Publications (2)

Publication Number Publication Date
WO2000071987A2 WO2000071987A2 (en) 2000-11-30
WO2000071987A3 true WO2000071987A3 (en) 2001-04-19

Family

ID=8240233

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2000/000281 WO2000071987A2 (en) 1999-05-25 2000-05-25 Isolation and culturing of fetal cells

Country Status (3)

Country Link
AU (1) AU4745200A (en)
CA (1) CA2413835A1 (en)
WO (1) WO2000071987A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9447467B2 (en) 2009-04-21 2016-09-20 Genetic Technologies Limited Methods for obtaining fetal genetic material
EP2516677B1 (en) 2009-12-23 2014-11-26 Genetic Technologies Limited Methods of enriching and detecting fetal nucleic acids
EP4008270A1 (en) 2012-06-22 2022-06-08 Preprogen LLC Method for obtaining fetal cells and fetal cellular components

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995009245A1 (en) * 1993-09-27 1995-04-06 Oncor, Inc. Methods for detecting and analyzing individual rare cells in a population
US5447842A (en) * 1990-03-27 1995-09-05 Genetype A.G. Fetal cell recovery method
WO1996027132A1 (en) * 1995-02-28 1996-09-06 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof
EP0791659A2 (en) * 1989-11-13 1997-08-27 Children's Medical Center Corporation Antigen specific separation of nucleated fetal cells in non-invasive detection of fetal DNA
US5731156A (en) * 1996-10-21 1998-03-24 Applied Imaging, Inc. Use of anti-embryonic hemoglobin antibodies to identify fetal cells
US5750339A (en) * 1994-11-30 1998-05-12 Thomas Jefferson University Methods for identifying fetal cells
WO1998040746A1 (en) * 1997-03-08 1998-09-17 The University Of Dundee Prenatal diagnostic methods
US5858649A (en) * 1992-07-17 1999-01-12 Aprogenex, Inc. Amplification of mRNA for distinguishing fetal cells in maternal blood
WO1999041613A1 (en) * 1998-02-12 1999-08-19 Immunivest Methods and reagents for the rapid and efficient isolation of circulating cancer cells
US5962234A (en) * 1997-10-20 1999-10-05 Applied Imaging Corporation Use of anti-embryonic epsilon hemoglobin antibodies to identify fetal cells

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0791659A2 (en) * 1989-11-13 1997-08-27 Children's Medical Center Corporation Antigen specific separation of nucleated fetal cells in non-invasive detection of fetal DNA
US5447842A (en) * 1990-03-27 1995-09-05 Genetype A.G. Fetal cell recovery method
US5858649A (en) * 1992-07-17 1999-01-12 Aprogenex, Inc. Amplification of mRNA for distinguishing fetal cells in maternal blood
WO1995009245A1 (en) * 1993-09-27 1995-04-06 Oncor, Inc. Methods for detecting and analyzing individual rare cells in a population
US5750339A (en) * 1994-11-30 1998-05-12 Thomas Jefferson University Methods for identifying fetal cells
WO1996027132A1 (en) * 1995-02-28 1996-09-06 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof
US5731156A (en) * 1996-10-21 1998-03-24 Applied Imaging, Inc. Use of anti-embryonic hemoglobin antibodies to identify fetal cells
WO1998040746A1 (en) * 1997-03-08 1998-09-17 The University Of Dundee Prenatal diagnostic methods
US5962234A (en) * 1997-10-20 1999-10-05 Applied Imaging Corporation Use of anti-embryonic epsilon hemoglobin antibodies to identify fetal cells
WO1999041613A1 (en) * 1998-02-12 1999-08-19 Immunivest Methods and reagents for the rapid and efficient isolation of circulating cancer cells

Also Published As

Publication number Publication date
AU4745200A (en) 2000-12-12
CA2413835A1 (en) 2000-11-30
WO2000071987A2 (en) 2000-11-30

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